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Protein

Syntaxin-12

Gene

STX12

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • autophagosome assembly Source: ParkinsonsUK-UCL
  • cholesterol efflux Source: BHF-UCL
  • intracellular protein transport Source: GO_Central
  • protein stabilization Source: BHF-UCL
  • vesicle docking Source: GO_Central
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Syntaxin-12
Gene namesi
Name:STX12
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:11430. STX12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 248247CytoplasmicSequence analysisAdd
BLAST
Transmembranei249 – 26921Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini270 – 2767VesicularSequence analysis

GO - Cellular componenti

  • endomembrane system Source: GO_Central
  • endosome membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: GO_Central
  • membrane raft Source: BHF-UCL
  • phagocytic vesicle Source: BHF-UCL
  • pre-autophagosomal structure Source: ParkinsonsUK-UCL
  • SNARE complex Source: UniProtKB
  • vesicle Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA36230.

Polymorphism and mutation databases

BioMutaiSTX12.
DMDMi47117211.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 276275Syntaxin-12PRO_0000210223Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei139 – 1391PhosphoserineBy similarity
Modified residuei142 – 1421PhosphoserineCombined sources
Modified residuei218 – 2181PhosphoserineBy similarity
Modified residuei225 – 2251PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ86Y82.
MaxQBiQ86Y82.
PaxDbiQ86Y82.
PeptideAtlasiQ86Y82.
PRIDEiQ86Y82.

PTM databases

iPTMnetiQ86Y82.
PhosphoSiteiQ86Y82.
SwissPalmiQ86Y82.

Miscellaneous databases

PMAP-CutDBQ86Y82.

Expressioni

Gene expression databases

BgeeiENSG00000117758.
CleanExiHS_STX12.
ExpressionAtlasiQ86Y82. baseline and differential.
GenevisibleiQ86Y82. HS.

Organism-specific databases

HPAiHPA041038.
HPA055300.

Interactioni

Subunit structurei

Interacts with NAPA and SNAP23. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity). Associates with the BLOC-1 complex. Interacts with BLOC1S6.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ABCA1O954779EBI-2691717,EBI-784112
DTX2Q4ZH493EBI-2691717,EBI-10192429
SNAP29O957214EBI-2691717,EBI-490676
STX4Q128463EBI-2691717,EBI-744942

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117191. 92 interactions.
DIPiDIP-44225N.
IntActiQ86Y82. 16 interactions.
MINTiMINT-3036147.
STRINGi9606.ENSP00000363054.

Structurei

Secondary structure

1
276
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi22 – 5029Combined sources
Beta strandi52 – 543Combined sources
Helixi57 – 8630Combined sources
Helixi93 – 13240Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNXNMR-A18-134[»]
ProteinModelPortaliQ86Y82.
SMRiQ86Y82. Positions 15-139, 184-248.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86Y82.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 24063t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili33 – 13199Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the syntaxin family.Curated
Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00450000040239.
HOGENOMiHOG000188453.
HOVERGENiHBG053083.
InParanoidiQ86Y82.
KOiK13813.
OMAiQMQSQEE.
OrthoDBiEOG091G0HKZ.
PhylomeDBiQ86Y82.
TreeFamiTF315607.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86Y82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYGPLDMYR NPGPSGPQLR DFSSIIQTCS GNIQRISQAT AQIKNLMSQL
60 70 80 90 100
GTKQDSSKLQ ENLQQLQHST NQLAKETNEL LKELGSLPLP LSTSEQRQQR
110 120 130 140 150
LQKERLMNDF SAALNNFQAV QRRVSEKEKE SIARARAGSR LSAEERQREE
160 170 180 190 200
QLVSFDSHEE WNQMQSQEDE VAITEQDLEL IKERETAIRQ LEADILDVNQ
210 220 230 240 250
IFKDLAMMIH DQGDLIDSIE ANVESSEVHV ERATEQLQRA AYYQKKSRKK
260 270
MCILVLVLSV IILILGLIIW LVYKTK
Length:276
Mass (Da):31,642
Last modified:June 1, 2003 - v1
Checksum:i2338061572ED7DA1
GO

Sequence cautioni

The sequence CAA22911 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti88 – 881P → R in a breast cancer sample; somatic mutation. 1 Publication
VAR_035643

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123769 mRNA. Translation: AAP97248.1.
AL035306 mRNA. Translation: CAA22911.1. Different initiation.
AK297585 mRNA. Translation: BAG59973.1.
AL020997 Genomic DNA. Translation: CAI20575.1.
CH471059 Genomic DNA. Translation: EAX07738.1.
CH471059 Genomic DNA. Translation: EAX07739.1.
BC046999 mRNA. Translation: AAH46999.1.
CCDSiCCDS310.1.
RefSeqiNP_803173.1. NM_177424.2.
UniGeneiHs.523855.

Genome annotation databases

EnsembliENST00000373943; ENSP00000363054; ENSG00000117758.
GeneIDi23673.
KEGGihsa:23673.
UCSCiuc001bou.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123769 mRNA. Translation: AAP97248.1.
AL035306 mRNA. Translation: CAA22911.1. Different initiation.
AK297585 mRNA. Translation: BAG59973.1.
AL020997 Genomic DNA. Translation: CAI20575.1.
CH471059 Genomic DNA. Translation: EAX07738.1.
CH471059 Genomic DNA. Translation: EAX07739.1.
BC046999 mRNA. Translation: AAH46999.1.
CCDSiCCDS310.1.
RefSeqiNP_803173.1. NM_177424.2.
UniGeneiHs.523855.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNXNMR-A18-134[»]
ProteinModelPortaliQ86Y82.
SMRiQ86Y82. Positions 15-139, 184-248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117191. 92 interactions.
DIPiDIP-44225N.
IntActiQ86Y82. 16 interactions.
MINTiMINT-3036147.
STRINGi9606.ENSP00000363054.

PTM databases

iPTMnetiQ86Y82.
PhosphoSiteiQ86Y82.
SwissPalmiQ86Y82.

Polymorphism and mutation databases

BioMutaiSTX12.
DMDMi47117211.

Proteomic databases

EPDiQ86Y82.
MaxQBiQ86Y82.
PaxDbiQ86Y82.
PeptideAtlasiQ86Y82.
PRIDEiQ86Y82.

Protocols and materials databases

DNASUi23673.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000373943; ENSP00000363054; ENSG00000117758.
GeneIDi23673.
KEGGihsa:23673.
UCSCiuc001bou.4. human.

Organism-specific databases

CTDi23673.
GeneCardsiSTX12.
HGNCiHGNC:11430. STX12.
HPAiHPA041038.
HPA055300.
MIMi606892. gene.
neXtProtiNX_Q86Y82.
PharmGKBiPA36230.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0811. Eukaryota.
COG5325. LUCA.
GeneTreeiENSGT00450000040239.
HOGENOMiHOG000188453.
HOVERGENiHBG053083.
InParanoidiQ86Y82.
KOiK13813.
OMAiQMQSQEE.
OrthoDBiEOG091G0HKZ.
PhylomeDBiQ86Y82.
TreeFamiTF315607.

Miscellaneous databases

ChiTaRSiSTX12. human.
EvolutionaryTraceiQ86Y82.
GeneWikiiSTX12.
GenomeRNAii23673.
PMAP-CutDBQ86Y82.
PROiQ86Y82.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000117758.
CleanExiHS_STX12.
ExpressionAtlasiQ86Y82. baseline and differential.
GenevisibleiQ86Y82. HS.

Family and domain databases

InterProiIPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF05739. SNARE. 1 hit.
[Graphical view]
SMARTiSM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]
SUPFAMiSSF47661. SSF47661. 1 hit.
PROSITEiPS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTX12_HUMAN
AccessioniPrimary (citable) accession number: Q86Y82
Secondary accession number(s): B1AJQ7, O95564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.