Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q86Y82

- STX12_HUMAN

UniProt

Q86Y82 - STX12_HUMAN

Protein

Syntaxin-12

Gene

STX12

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro) By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. SNAP receptor activity Source: RefGenome
    3. SNARE binding Source: RefGenome

    GO - Biological processi

    1. cholesterol efflux Source: BHF-UCL
    2. intracellular protein transport Source: RefGenome
    3. protein stabilization Source: BHF-UCL
    4. synaptic vesicle exocytosis Source: RefGenome

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-12
    Gene namesi
    Name:STX12
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:11430. STX12.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. extracellular vesicular exosome Source: UniProt
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. membrane raft Source: BHF-UCL
    6. phagocytic vesicle Source: BHF-UCL
    7. SNARE complex Source: UniProtKB

    Keywords - Cellular componenti

    Endosome, Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36230.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 276275Syntaxin-12PRO_0000210223Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications
    Modified residuei139 – 1391PhosphoserineBy similarity
    Modified residuei142 – 1421Phosphoserine1 Publication
    Modified residuei218 – 2181PhosphoserineBy similarity
    Modified residuei225 – 2251PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ86Y82.
    PaxDbiQ86Y82.
    PRIDEiQ86Y82.

    PTM databases

    PhosphoSiteiQ86Y82.

    Miscellaneous databases

    PMAP-CutDBQ86Y82.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86Y82.
    BgeeiQ86Y82.
    CleanExiHS_STX12.
    GenevestigatoriQ86Y82.

    Organism-specific databases

    HPAiHPA041038.

    Interactioni

    Subunit structurei

    Interacts with NAPA and SNAP23. Identified in a complex containing STX6, STX12, VAMP4 and VTI1A By similarity. Associates with the BLOC-1 complex. Interacts with BLOC1S6.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi117191. 16 interactions.
    IntActiQ86Y82. 6 interactions.
    MINTiMINT-3036147.
    STRINGi9606.ENSP00000363054.

    Structurei

    Secondary structure

    1
    276
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi22 – 5029
    Beta strandi52 – 543
    Helixi57 – 8630
    Helixi93 – 13240

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DNXNMR-A18-134[»]
    ProteinModelPortaliQ86Y82.
    SMRiQ86Y82. Positions 15-139, 184-248.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ86Y82.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 248247CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini270 – 2767VesicularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei249 – 26921Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini178 – 24063t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili33 – 13199Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the syntaxin family.Curated
    Contains 1 t-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5325.
    HOGENOMiHOG000188453.
    HOVERGENiHBG053083.
    KOiK13813.
    OMAiDGHEEWN.
    OrthoDBiEOG7B31PS.
    PhylomeDBiQ86Y82.
    TreeFamiTF315607.

    Family and domain databases

    InterProiIPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view]
    PfamiPF05739. SNARE. 1 hit.
    [Graphical view]
    SMARTiSM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view]
    SUPFAMiSSF47661. SSF47661. 1 hit.
    PROSITEiPS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q86Y82-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYGPLDMYR NPGPSGPQLR DFSSIIQTCS GNIQRISQAT AQIKNLMSQL    50
    GTKQDSSKLQ ENLQQLQHST NQLAKETNEL LKELGSLPLP LSTSEQRQQR 100
    LQKERLMNDF SAALNNFQAV QRRVSEKEKE SIARARAGSR LSAEERQREE 150
    QLVSFDSHEE WNQMQSQEDE VAITEQDLEL IKERETAIRQ LEADILDVNQ 200
    IFKDLAMMIH DQGDLIDSIE ANVESSEVHV ERATEQLQRA AYYQKKSRKK 250
    MCILVLVLSV IILILGLIIW LVYKTK 276
    Length:276
    Mass (Da):31,642
    Last modified:June 1, 2003 - v1
    Checksum:i2338061572ED7DA1
    GO

    Sequence cautioni

    The sequence CAA22911.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti88 – 881P → R in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035643

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF123769 mRNA. Translation: AAP97248.1.
    AL035306 mRNA. Translation: CAA22911.1. Different initiation.
    AK297585 mRNA. Translation: BAG59973.1.
    AL020997 Genomic DNA. Translation: CAI20575.1.
    CH471059 Genomic DNA. Translation: EAX07738.1.
    CH471059 Genomic DNA. Translation: EAX07739.1.
    BC046999 mRNA. Translation: AAH46999.1.
    CCDSiCCDS310.1.
    RefSeqiNP_803173.1. NM_177424.2.
    UniGeneiHs.523855.

    Genome annotation databases

    EnsembliENST00000373943; ENSP00000363054; ENSG00000117758.
    GeneIDi23673.
    KEGGihsa:23673.
    UCSCiuc001bou.4. human.

    Polymorphism databases

    DMDMi47117211.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF123769 mRNA. Translation: AAP97248.1 .
    AL035306 mRNA. Translation: CAA22911.1 . Different initiation.
    AK297585 mRNA. Translation: BAG59973.1 .
    AL020997 Genomic DNA. Translation: CAI20575.1 .
    CH471059 Genomic DNA. Translation: EAX07738.1 .
    CH471059 Genomic DNA. Translation: EAX07739.1 .
    BC046999 mRNA. Translation: AAH46999.1 .
    CCDSi CCDS310.1.
    RefSeqi NP_803173.1. NM_177424.2.
    UniGenei Hs.523855.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DNX NMR - A 18-134 [» ]
    ProteinModelPortali Q86Y82.
    SMRi Q86Y82. Positions 15-139, 184-248.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117191. 16 interactions.
    IntActi Q86Y82. 6 interactions.
    MINTi MINT-3036147.
    STRINGi 9606.ENSP00000363054.

    PTM databases

    PhosphoSitei Q86Y82.

    Polymorphism databases

    DMDMi 47117211.

    Proteomic databases

    MaxQBi Q86Y82.
    PaxDbi Q86Y82.
    PRIDEi Q86Y82.

    Protocols and materials databases

    DNASUi 23673.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000373943 ; ENSP00000363054 ; ENSG00000117758 .
    GeneIDi 23673.
    KEGGi hsa:23673.
    UCSCi uc001bou.4. human.

    Organism-specific databases

    CTDi 23673.
    GeneCardsi GC01P028099.
    HGNCi HGNC:11430. STX12.
    HPAi HPA041038.
    MIMi 606892. gene.
    neXtProti NX_Q86Y82.
    PharmGKBi PA36230.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5325.
    HOGENOMi HOG000188453.
    HOVERGENi HBG053083.
    KOi K13813.
    OMAi DGHEEWN.
    OrthoDBi EOG7B31PS.
    PhylomeDBi Q86Y82.
    TreeFami TF315607.

    Miscellaneous databases

    ChiTaRSi STX12. human.
    EvolutionaryTracei Q86Y82.
    GeneWikii STX12.
    GenomeRNAii 23673.
    NextBioi 46531.
    PMAP-CutDB Q86Y82.
    PROi Q86Y82.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86Y82.
    Bgeei Q86Y82.
    CleanExi HS_STX12.
    Genevestigatori Q86Y82.

    Family and domain databases

    InterProi IPR006012. Syntaxin/epimorphin_CS.
    IPR006011. Syntaxin_N.
    IPR010989. t-SNARE.
    IPR000727. T_SNARE_dom.
    [Graphical view ]
    Pfami PF05739. SNARE. 1 hit.
    [Graphical view ]
    SMARTi SM00503. SynN. 1 hit.
    SM00397. t_SNARE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47661. SSF47661. 1 hit.
    PROSITEi PS00914. SYNTAXIN. 1 hit.
    PS50192. T_SNARE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning a new human cDNA homologous to R.norvegicus syntaxin 12 mRNA."
      Yang J., Yu L., Zhao S.Y.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Rhodes S.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth."
      Ghiani C.A., Starcevic M., Rodriguez-Fernandez I.A., Nazarian R., Cheli V.T., Chan L.N., Malvar J.S., de Vellis J., Sabatti C., Dell'Angelica E.C.
      Mol. Psychiatry 15:204-215(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE BLOC-1 COMPLEX, INTERACTION WITH BLOC1S6.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Solution structure of RSGI RUH-063, an N-terminal domain of syntaxin 12 from human cDNA."
      RIKEN structural genomics initiative (RSGI)
      Submitted (OCT-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 18-134.
    14. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-88.

    Entry informationi

    Entry nameiSTX12_HUMAN
    AccessioniPrimary (citable) accession number: Q86Y82
    Secondary accession number(s): B1AJQ7, O95564
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3