Q86Y26 (NUT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NUT

Alternative name(s):
Nuclear protein in testis

Gene names

Name:	NUT
Synonyms:	C15orf55

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1132 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm. Nucleus. Note: Shuttles between nucleus and cytoplasm. Ref.7
Tissue specificity	Specifically expressed in testis. Ref.1
Post-translational modification	Phosphorylation on Ser-1026, Ser-1029 or Ser-1031 is important for cytoplasmic export.
Involvement in disease	A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with BRD4 which produces a BRD4-NUT fusion protein. Ref.1 A chromosomal aberration involving NUT is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with BRD3 which produces a BRD3-NUT fusion protein.
Sequence similarities	Belongs to the FAM22 family.
Sequence caution	The sequence AAO22237.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Chromosomal rearrangement Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86Y26-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86Y26-2)

The sequence of this isoform differs from the canonical sequence as follows:
     235-365: Missing.
     565-573: LQPELAAPQ → PPTNGESQC
     574-1132: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1132	1132	Protein NUT		PRO_0000311394
Regions
	Compositional bias	10 – 182	173	Pro-rich
Sites
	Site	5 – 6	2	Breakpoint for translocation to form BRD4-NUT and BRD3-NUT fusion proteins
Amino acid modifications
	Modified residue	1026	1	Phosphoserine Probable
	Modified residue	1029	1	Phosphoserine Probable
	Modified residue	1031	1	Phosphoserine Probable
Natural variations
	Alternative sequence	235 – 365	131	Missing in isoform 2.		VSP_029559
	Alternative sequence	565 – 573	9	LQPELAAPQ → PPTNGESQC in isoform 2.		VSP_029560
	Alternative sequence	574 – 1132	559	Missing in isoform 2.		VSP_029561
	Natural variant	22	1	P → L. Ref.1 Ref.2 Ref.3 Ref.5 Corresponds to variant rs374230 [ dbSNP | Ensembl ].		VAR_037239
	Natural variant	781	1	T → M. Corresponds to variant rs16959028 [ dbSNP | Ensembl ].		VAR_037240
	Natural variant	785	1	V → E. Corresponds to variant rs17236868 [ dbSNP | Ensembl ].		VAR_037241
	Natural variant	973	1	T → N. Corresponds to variant rs2279683 [ dbSNP | Ensembl ].		VAR_037242
	Natural variant	985	1	P → R. Corresponds to variant rs2279684 [ dbSNP | Ensembl ].		VAR_037243
	Natural variant	1113	1	R → H. Corresponds to variant rs2279685 [ dbSNP | Ensembl ].		VAR_037244

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: 5CB58C1B030399F4	FASTA	1,132	120,314
	10 20 30 40 50 60 MASDGASALP GPDMSMKPSA APSPSPALPF LPPTSDPPDH PPREPPPQPI MPSVFSPDNP 70 80 90 100 110 120 LMLSAFPSSL LVTGDGGPCL SGAGAGKVIV KVKTEGGSAE PSQTQNFILT QTALNSTAPG 130 140 150 160 170 180 TPCGGLEGPA PPFVTASNVK TILPSKAVGV SQEGPPGLPP QPPPPVAQLV PIVPLEKAWP 190 200 210 220 230 240 GPHGTTGEGG PVATLSKPSL GDRSKISKDV YENFRQWQRY KALARRHLSQ SPDTEALSCF 250 260 270 280 290 300 LIPVLRSLAR LKPTMTLEEG LPLAVQEWEH TSNFDRMIFY EMAERFMEFE AEEMQIQNTQ 310 320 330 340 350 360 LMNGSQGLSP ATPLKLDPLG PLASEVCQQP VYIPKKAASK TRAPRRRQRK AQRPPAPEAP 370 380 390 400 410 420 KEIPPEAVKE YVDIMEWLVG THLATGESDG KQEEEGQQQE EEGMYPDPGL LSYINELCSQ 430 440 450 460 470 480 KVFVSKVEAV IHPQFLADLL SPEKQRDPLA LIEELEQEEG LTLAQLVQKR LMALEEEEDA 490 500 510 520 530 540 EAPPSFSGAQ LDSSPSGSVE DEDGDGRLRP SPGLQGAGGA ACLGKVSSSG KRAREVHGGQ 550 560 570 580 590 600 EQALDSPRGM HRDGNTLPSP SSWDLQPELA APQGTPGPLG VERRGSGKVI NQVSLHQDGH 610 620 630 640 650 660 LGGAGPPGHC LVADRTSEAL PLCWQGGFQP ESTPSLDAGL AELAPLQGQG LEKQVLGLQK 670 680 690 700 710 720 GQQTGGRGVL PQGKEPLAVP WEGSSGAMWG DDRGTPMAQS YDQNPSPRAA GERDDVCLSP 730 740 750 760 770 780 GVWLSSEMDA VGLELPVQIE EVIESFQVEK CVTEYQEGCQ GLGSRGNISL GPGETLVPGD 790 800 810 820 830 840 TESSVIPCGG TVAAAALEKR NYCSLPGPLR ANSPPLRSKE NQEQSCETVG HPSDLWAEGC 850 860 870 880 890 900 FPLLESGDST LGSSKETLPP TCQGNLLIMG TEDASSLPEA SQEAGSRGNS FSPLLETIEP 910 920 930 940 950 960 VNILDVKDDC GLQLRVSEDT CPLNVHSYDP QGEGRVDPDL SKPKNLAPLQ ESQESYTTGT 970 980 990 1000 1010 1020 PKATSSHQGL GSTLPRRGTR NAIVPRETSV SKTHRSADRA KGKEKKKKEA EEEDEELSNF 1030 1040 1050 1060 1070 1080 AYLLASKLSL SPREHPLSPH HASGGQGSQR ASHLLPAGAK GPSKLPYPVA KSGKRALAGG 1090 1100 1110 1120 1130 PAPTEKTPHS GAQLGVPREK PLALGVVRPS QPRKRRCDSF VTGRRKKRRR SQ
	Isoform 2 [UniParc]. Checksum: 95BABF1664CEE540	FASTA	442	46,352
	10 20 30 40 50 60 MASDGASALP GPDMSMKPSA APSPSPALPF LPPTSDPPDH PPREPPPQPI MPSVFSPDNP 70 80 90 100 110 120 LMLSAFPSSL LVTGDGGPCL SGAGAGKVIV KVKTEGGSAE PSQTQNFILT QTALNSTAPG 130 140 150 160 170 180 TPCGGLEGPA PPFVTASNVK TILPSKAVGV SQEGPPGLPP QPPPPVAQLV PIVPLEKAWP 190 200 210 220 230 240 GPHGTTGEGG PVATLSKPSL GDRSKISKDV YENFRQWQRY KALARRHLSQ SPDTEAVKEY 250 260 270 280 290 300 VDIMEWLVGT HLATGESDGK QEEEGQQQEE EGMYPDPGLL SYINELCSQK VFVSKVEAVI 310 320 330 340 350 360 HPQFLADLLS PEKQRDPLAL IEELEQEEGL TLAQLVQKRL MALEEEEDAE APPSFSGAQL 370 380 390 400 410 420 DSSPSGSVED EDGDGRLRPS PGLQGAGGAA CLGKVSSSGK RAREVHGGQE QALDSPRGMH 430 440 RDGNTLPSPS SWDPPTNGES QC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"BRD4-NUT fusion oncogene: a novel mechanism in aggressive carcinoma." French C.A., Miyoshi I., Kubonishi I., Grier H.E., Perez-Atayde A.R., Fletcher J.A. Cancer Res. 63:304-307(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DISEASE, CHROMOSOMAL TRANSLOCATION WITH BRD4, TISSUE SPECIFICITY, VARIANT LEU-22. Tissue: Carcinoma and Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-22. Tissue: Testis.
[3]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT LEU-22.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT LEU-22.
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 890-1132 (ISOFORM 1). Tissue: Testis.
[7]	"BRD-NUT oncoproteins: a family of closely related nuclear proteins that block epithelial differentiation and maintain the growth of carcinoma cells." French C.A., Ramirez C.L., Kolmakova J., Hickman T.T., Cameron M.J., Thyne M.E., Kutok J.L., Toretsky J.A., Tadavarthy A.K., Kees U.R., Fletcher J.A., Aster J.C. Oncogene 27:2237-2242(2008) [PubMed] [Europe PMC] [Abstract] Cited for: CHROMOSOMAL TRANSLOCATION WITH BRD3, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-1026; SER-1029 AND SER-1031.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF482429 mRNA. Translation: AAO39268.1. AY166680 mRNA. Translation: AAO22237.1. Different initiation. AK098568 mRNA. Translation: BAC05337.1. AC021822 Genomic DNA. No translation available. AC025678 Genomic DNA. No translation available. CH471125 Genomic DNA. Translation: EAW92304.1. BC114518 mRNA. Translation: AAI14519.1. BC114567 mRNA. Translation: AAI14568.1. AL137416 mRNA. Translation: CAB70729.1.
IPI	IPI00167192. IPI01010333.
PIR	T46461.
RefSeq	NP_786883.1. NM_175741.1.
UniGene	Hs.525769.
3D structure databases
ProteinModelPortal	Q86Y26.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000329448.
PTM databases
PhosphoSite	Q86Y26.
Polymorphism databases
DMDM	311033405.
Proteomic databases
PaxDb	Q86Y26.
PRIDE	Q86Y26.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333756; ENSP00000329448; ENSG00000184507.
GeneID	256646.
KEGG	hsa:256646.
Organism-specific databases
CTD	256646.
GeneCards	GC15P034635.
HGNC	HGNC:29919. C15orf55.
HPA	HPA040421.
MIM	608963. gene.
neXtProt	NX_Q86Y26.
PharmGKB	PA162378206.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39838.
HOVERGEN	HBG107898.
InParanoid	Q86Y26.
PhylomeDB	Q86Y26.
Gene expression databases
ArrayExpress	Q86Y26.
Bgee	Q86Y26.
CleanEx	HS_C15orf55.
Genevestigator	Q86Y26.
Family and domain databases
InterPro	IPR024310. NUT/FAM22. IPR024313. NUT_C. IPR024309. NUT_N. [Graphical view]
PANTHER	PTHR22879. PTHR22879. 1 hit.
Pfam	PF12882. NUT_C. 1 hit. PF12881. NUT_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	256646.
NextBio	92844.
SOURCE	Search...

Entry information

Entry name

NUT_HUMAN

Accession

Primary (citable) accession number: Q86Y26
Secondary accession number(s): Q86YS8, Q8N7F2, Q9NTB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 2, 2010
Last modified:	April 3, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.