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Protein

E3 ubiquitin-protein ligase DZIP3

Gene

DZIP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1148 – 1188RING-type; atypicalPROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphatase binding Source: UniProtKB
  • polyubiquitin binding Source: UniProtKB
  • RNA binding Source: UniProtKB
  • ubiquitin protein ligase activity involved in ERAD pathway Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB
  • ubiquitin-specific protease binding Source: GO_Central
  • unfolded protein binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DZIP3 (EC:2.3.2.271 Publication)
Alternative name(s):
DAZ-interacting protein 3
RING-type E3 ubiquitin transferase DZIP3Curated
RNA-binding ubiquitin ligase of 138 kDa
Short name:
hRUL138
Gene namesi
Name:DZIP3
Synonyms:KIAA0675
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:30938. DZIP3.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi662 – 666KKKTK → SGSTA: Strongly decreases RNA-binding activity. 1 Publication5
Mutagenesisi1187C → S: Abolishes ubiquitin ligase activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000198919.
PharmGKBiPA162384137.

Polymorphism and mutation databases

BioMutaiDZIP3.
DMDMi50400482.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558981 – 1208E3 ubiquitin-protein ligase DZIP3Add BLAST1208

Proteomic databases

EPDiQ86Y13.
MaxQBiQ86Y13.
PaxDbiQ86Y13.
PeptideAtlasiQ86Y13.
PRIDEiQ86Y13.

PTM databases

iPTMnetiQ86Y13.
PhosphoSitePlusiQ86Y13.

Expressioni

Tissue specificityi

Widely expressed at low level. Highly expressed in skeletal muscle, kidney and heart. Expressed at low level in placenta, lung, brain, liver and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000198919.
ExpressionAtlasiQ86Y13. baseline and differential.
GenevisibleiQ86Y13. HS.

Organism-specific databases

HPAiHPA035066.
HPA049232.

Interactioni

Subunit structurei

Interacts with DAZ proteins.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • phosphatase binding Source: UniProtKB
  • polyubiquitin binding Source: UniProtKB
  • ubiquitin-specific protease binding Source: GO_Central
  • unfolded protein binding Source: GO_Central

Protein-protein interaction databases

BioGridi115021. 52 interactors.
IntActiQ86Y13. 47 interactors.
MINTiMINT-2867156.
STRINGi9606.ENSP00000355028.

Structurei

3D structure databases

ProteinModelPortaliQ86Y13.
SMRiQ86Y13.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili14 – 43Sequence analysisAdd BLAST30
Coiled coili647 – 676Sequence analysisAdd BLAST30
Coiled coili792 – 853Sequence analysisAdd BLAST62
Coiled coili904 – 939Sequence analysisAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi607 – 610Poly-Glu4
Compositional biasi661 – 671Poly-LysAdd BLAST11
Compositional biasi952 – 958Poly-Pro7
Compositional biasi1140 – 1146Poly-Glu7

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1148 – 1188RING-type; atypicalPROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IFEY. Eukaryota.
ENOG410Y41H. LUCA.
GeneTreeiENSGT00530000063254.
HOVERGENiHBG051428.
InParanoidiQ86Y13.
KOiK10642.
OMAiRLCKYRD.
OrthoDBiEOG091G0YWM.
PhylomeDBiQ86Y13.
TreeFamiTF333981.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR033103. DZIP3.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR22763:SF121. PTHR22763:SF121. 1 hit.
PfamiView protein in Pfam
PF13639. zf-RING_2. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86Y13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLPDEFFV RHPAVEDQRK EETENKLEKS SGQLNKQEND IPTDLVPVNL
60 70 80 90 100
LLEVKKLLNA INTLPKGVVP HIKKFLQEDF SFQTMQREVA ANSQNGEEIV
110 120 130 140 150
PALTLRFLIT QLEAALRNIQ AGNYTAHQIN IGYYLTLLFL YGVALTERGK
160 170 180 190 200
KEDYTEAENK FLVMKMMIQE NEICENFMSL VYFGRGLLRC AQKRYNGGLL
210 220 230 240 250
EFHKSLQEIG DKNDHWFDID PTEDEDLPTT FKDLLNNFIK TTESNIMKQT
260 270 280 290 300
ICSYLDCERS CEADILKNTS YKGFFQLMCS KSCCVYFHKI CWKKFKNLKY
310 320 330 340 350
PGENDQSFSG KKCLKEGCTG DMVRMLQCDV PGIVKILFEV VRKDEYITIE
360 370 380 390 400
NLGASYRKLI SLKITDTDIR PKISLKFNTK DEMPIFKLDY NYFYHLLHII
410 420 430 440 450
IISGTDIVRQ IFDEAMPPPL LKKELLIHKN VLESYYNHLW TNHPLGGSWH
460 470 480 490 500
LLYPPNKELP QSKQFDLCLL LALIKHLNVF PAPKKGWNME PPSSDISKSA
510 520 530 540 550
DILRLCKYRD ILLSEILMNG LTESQFNSIW KKVSDILLRL GMMQEDIDKV
560 570 580 590 600
KENPIENISL DYHQLSVYLG IPVPEIIQRM LSCYQQGIAL QSITGSQRIE
610 620 630 640 650
IEELQNEEEE LSPPLMEYNI NVKSHPEIQF AEINKDGTSI PSESSTESLK
660 670 680 690 700
DLQEVKSKQR KKKKTKNKKN KDSKEDQVPY VVEKEEQLRK EQANPHSVSR
710 720 730 740 750
LIKDDASDVQ EDSAMEDKFY SLDELHILDM IEQGSAGKVT TDYGETEKER
760 770 780 790 800
LARQRQLYKL HYQCEDFKRQ LRTVTFRWQE NQMQIKKKDK IIASLNQQVA
810 820 830 840 850
FGINKVSKLQ RQIHAKDNEI KNLKEQLSMK RSQWEMEKHN LESTMKTYVS
860 870 880 890 900
KLNAETSRAL TAEVYFLQCR RDFGLLHLEQ TEKECLNQLA RVTHMAASNL
910 920 930 940 950
ESLQLKAAVD SWNAIVADVR NKIAFLRTQY NEQINKVKQG FALSTLPPVQ
960 970 980 990 1000
LPPPPPSPEI LMQQFLGRPL VKESFFRPIL TVPQMPAVCP GVVSATGQPR
1010 1020 1030 1040 1050
APLMTGIAWA LPAPVGDAVP PSAGLRSDPS IMNWERITDR LKTAFPQQTR
1060 1070 1080 1090 1100
KELTDFLRKL KDAYGKSLSE LTFDEIVCKI SQFIDPKKSQ SQGKSVSNVN
1110 1120 1130 1140 1150
CVSPSHSPSQ PDAAQPPKPA WRPLTSQGPA TWEGASNPDE EEEEEEPCVI
1160 1170 1180 1190 1200
CHENLSPENL SVLPCAHKFH AQCIRPWLMQ QGTCPTCRLH VLLPEEFPGH

PSRQLPKI
Length:1,208
Mass (Da):138,604
Last modified:July 19, 2004 - v2
Checksum:i76945A63AF85207E
GO
Isoform 2 (identifier: Q86Y13-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: GE → EF
     304-1208: Missing.

Show »
Length:303
Mass (Da):35,240
Checksum:iAACC1AF978138456
GO

Sequence cautioni

The sequence AAH39018 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH56674 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAK69484 differs from that shown. Reason: Frameshift at position 1127.Curated
The sequence BAA31650 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti368D → G in AAH56674 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010971302 – 303GE → EF in isoform 2. Curated2
Alternative sequenceiVSP_010972304 – 1208Missing in isoform 2. CuratedAdd BLAST905

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY227651 mRNA. Translation: AAO72967.1.
AY227652 mRNA. Translation: AAO72968.1.
AY227653 Transcribed RNA. Translation: AAO72969.1.
AY227654 mRNA. Translation: AAO72970.1.
AB014575 mRNA. Translation: BAA31650.2. Different initiation.
AK023138 mRNA. Translation: BAG51163.1.
BC039018 mRNA. Translation: AAH39018.1. Sequence problems.
BC056674 mRNA. Translation: AAH56674.1. Sequence problems.
BC063882 mRNA. Translation: AAH63882.1.
AF279370 mRNA. Translation: AAK69484.1. Frameshift.
CCDSiCCDS2952.1. [Q86Y13-1]
PIRiT00362.
RefSeqiNP_055463.1. NM_014648.3. [Q86Y13-1]
XP_005247971.1. XM_005247914.3. [Q86Y13-1]
XP_005247972.1. XM_005247915.3. [Q86Y13-1]
XP_005247973.1. XM_005247916.3. [Q86Y13-1]
XP_005247974.1. XM_005247917.3. [Q86Y13-1]
XP_005247975.1. XM_005247918.3. [Q86Y13-1]
XP_016863029.1. XM_017007540.1. [Q86Y13-1]
UniGeneiHs.409210.

Genome annotation databases

EnsembliENST00000361582; ENSP00000355028; ENSG00000198919. [Q86Y13-1]
ENST00000463306; ENSP00000419981; ENSG00000198919. [Q86Y13-1]
ENST00000495008; ENSP00000418871; ENSG00000198919. [Q86Y13-2]
GeneIDi9666.
KEGGihsa:9666.
UCSCiuc003dxd.4. human. [Q86Y13-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDZIP3_HUMAN
AccessioniPrimary (citable) accession number: Q86Y13
Secondary accession number(s): B3KN01
, O75162, Q6P3R9, Q6PH82, Q86Y14, Q86Y15, Q86Y16, Q8IWI0, Q96RS9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: August 30, 2017
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways