Q86Y13 (DZIP3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase DZIP3
EC=6.3.2.-

Alternative name(s):
DAZ-interacting protein 3
RNA-binding ubiquitin ligase of 138 kDa
Short name=hRUL138

Gene names

Name:	DZIP3
Synonyms:	KIAA0675

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DAZ proteins. Ref.5
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Widely expressed at low level. Highly expressed in skeletal muscle, kidney and heart. Expressed at low level in placenta, lung, brain, liver and pancreas. Ref.1
Sequence similarities	Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAH39018.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH56674.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAK69484.1 differs from that shown. Reason: Frameshift at position 1127. The sequence BAA31650.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein polyubiquitination Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	RNA binding Inferred from direct assay Ref.1. Source: UniProtKB phosphatase binding Inferred from direct assay. Source: UniProtKB polyubiquitin binding Inferred from direct assay Ref.1. Source: UniProtKB ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86Y13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86Y13-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: GE → EF
     304-1208: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1208	1208	E3 ubiquitin-protein ligase DZIP3		PRO_0000055898
Regions
	Zinc finger	1148 – 1188	41	RING-type; atypical
	Coiled coil	14 – 43	30	Potential
	Coiled coil	647 – 676	30	Potential
	Coiled coil	792 – 853	62	Potential
	Coiled coil	904 – 939	36	Potential
	Compositional bias	607 – 610	4	Poly-Glu
	Compositional bias	661 – 671	11	Poly-Lys
	Compositional bias	952 – 958	7	Poly-Pro
	Compositional bias	1140 – 1146	7	Poly-Glu
Natural variations
	Alternative sequence	302 – 303	2	GE → EF in isoform 2.		VSP_010971
	Alternative sequence	304 – 1208	905	Missing in isoform 2.		VSP_010972
Experimental info
	Mutagenesis	662 – 666	5	KKKTK → SGSTA: Strongly decreases RNA-binding activity. Ref.1
	Mutagenesis	1187	1	C → S: Abolishes ubiquitin ligase activity. Ref.1
	Sequence conflict	368	1	D → G in AAH56674. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 76945A63AF85207E	FASTA	1,208	138,604
	10 20 30 40 50 60 MDSLPDEFFV RHPAVEDQRK EETENKLEKS SGQLNKQEND IPTDLVPVNL LLEVKKLLNA 70 80 90 100 110 120 INTLPKGVVP HIKKFLQEDF SFQTMQREVA ANSQNGEEIV PALTLRFLIT QLEAALRNIQ 130 140 150 160 170 180 AGNYTAHQIN IGYYLTLLFL YGVALTERGK KEDYTEAENK FLVMKMMIQE NEICENFMSL 190 200 210 220 230 240 VYFGRGLLRC AQKRYNGGLL EFHKSLQEIG DKNDHWFDID PTEDEDLPTT FKDLLNNFIK 250 260 270 280 290 300 TTESNIMKQT ICSYLDCERS CEADILKNTS YKGFFQLMCS KSCCVYFHKI CWKKFKNLKY 310 320 330 340 350 360 PGENDQSFSG KKCLKEGCTG DMVRMLQCDV PGIVKILFEV VRKDEYITIE NLGASYRKLI 370 380 390 400 410 420 SLKITDTDIR PKISLKFNTK DEMPIFKLDY NYFYHLLHII IISGTDIVRQ IFDEAMPPPL 430 440 450 460 470 480 LKKELLIHKN VLESYYNHLW TNHPLGGSWH LLYPPNKELP QSKQFDLCLL LALIKHLNVF 490 500 510 520 530 540 PAPKKGWNME PPSSDISKSA DILRLCKYRD ILLSEILMNG LTESQFNSIW KKVSDILLRL 550 560 570 580 590 600 GMMQEDIDKV KENPIENISL DYHQLSVYLG IPVPEIIQRM LSCYQQGIAL QSITGSQRIE 610 620 630 640 650 660 IEELQNEEEE LSPPLMEYNI NVKSHPEIQF AEINKDGTSI PSESSTESLK DLQEVKSKQR 670 680 690 700 710 720 KKKKTKNKKN KDSKEDQVPY VVEKEEQLRK EQANPHSVSR LIKDDASDVQ EDSAMEDKFY 730 740 750 760 770 780 SLDELHILDM IEQGSAGKVT TDYGETEKER LARQRQLYKL HYQCEDFKRQ LRTVTFRWQE 790 800 810 820 830 840 NQMQIKKKDK IIASLNQQVA FGINKVSKLQ RQIHAKDNEI KNLKEQLSMK RSQWEMEKHN 850 860 870 880 890 900 LESTMKTYVS KLNAETSRAL TAEVYFLQCR RDFGLLHLEQ TEKECLNQLA RVTHMAASNL 910 920 930 940 950 960 ESLQLKAAVD SWNAIVADVR NKIAFLRTQY NEQINKVKQG FALSTLPPVQ LPPPPPSPEI 970 980 990 1000 1010 1020 LMQQFLGRPL VKESFFRPIL TVPQMPAVCP GVVSATGQPR APLMTGIAWA LPAPVGDAVP 1030 1040 1050 1060 1070 1080 PSAGLRSDPS IMNWERITDR LKTAFPQQTR KELTDFLRKL KDAYGKSLSE LTFDEIVCKI 1090 1100 1110 1120 1130 1140 SQFIDPKKSQ SQGKSVSNVN CVSPSHSPSQ PDAAQPPKPA WRPLTSQGPA TWEGASNPDE 1150 1160 1170 1180 1190 1200 EEEEEEPCVI CHENLSPENL SVLPCAHKFH AQCIRPWLMQ QGTCPTCRLH VLLPEEFPGH PSRQLPKI
	Isoform 2 (Short) [UniParc]. Checksum: AACC1AF978138456	FASTA	303	35,240
	10 20 30 40 50 60 MDSLPDEFFV RHPAVEDQRK EETENKLEKS SGQLNKQEND IPTDLVPVNL LLEVKKLLNA 70 80 90 100 110 120 INTLPKGVVP HIKKFLQEDF SFQTMQREVA ANSQNGEEIV PALTLRFLIT QLEAALRNIQ 130 140 150 160 170 180 AGNYTAHQIN IGYYLTLLFL YGVALTERGK KEDYTEAENK FLVMKMMIQE NEICENFMSL 190 200 210 220 230 240 VYFGRGLLRC AQKRYNGGLL EFHKSLQEIG DKNDHWFDID PTEDEDLPTT FKDLLNNFIK 250 260 270 280 290 300 TTESNIMKQT ICSYLDCERS CEADILKNTS YKGFFQLMCS KSCCVYFHKI CWKKFKNLKY PEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"hRUL138, a novel human RNA-binding RING-H2 ubiquitin-protein ligase." Kreft S.G., Nassal M. J. Cell Sci. 116:605-616(2003) [PubMed: 12538761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, TISSUE SPECIFICITY, MUTAGENESIS OF 662-LYS--LYS-666 AND CYS-1187. Tissue: Liver and Uterus.
[2]	"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro." Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin and Testis.
[5]	"Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins." Moore F.L., Jaruzelska J., Fox M.S., Urano J., Firpo M.T., Turek P.J., Dorfman D.M., Reijo Pera R.A. Proc. Natl. Acad. Sci. U.S.A. 100:538-543(2003) [PubMed: 12511597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 641-1208, INTERACTION WITH DAZ.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY227651 mRNA. Translation: AAO72967.1. AY227652 mRNA. Translation: AAO72968.1. AY227653 Transcribed RNA. Translation: AAO72969.1. AY227654 mRNA. Translation: AAO72970.1. AB014575 mRNA. Translation: BAA31650.2. Different initiation. AK023138 mRNA. Translation: BAG51163.1. BC039018 mRNA. Translation: AAH39018.1. Sequence problems. BC056674 mRNA. Translation: AAH56674.1. Sequence problems. BC063882 mRNA. Translation: AAH63882.1. AF279370 mRNA. Translation: AAK69484.1. Frameshift.
IPI	IPI00452463. IPI00945954.
PIR	T00362.
RefSeq	NP_055463.1. NM_014648.3.
UniGene	Hs.409210.
3D structure databases
ProteinModelPortal	Q86Y13.
SMR	Q86Y13. Positions 1143-1192.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86Y13. 22 interactions.
MINT	MINT-2867156.
STRING	Q86Y13.
PTM databases
PhosphoSite	Q86Y13.
Polymorphism databases
DMDM	50400482.
Proteomic databases
PRIDE	Q86Y13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361582; ENSP00000355028; ENSG00000198919. ENST00000463306; ENSP00000419981; ENSG00000198919.
GeneID	9666.
KEGG	hsa:9666.
UCSC	uc003dxd.1. human.
Organism-specific databases
CTD	9666.
GeneCards	GC03P108308.
H-InvDB	HIX0003531.
HGNC	HGNC:30938. DZIP3.
HPA	HPA035066.
MIM	608672. gene.
neXtProt	NX_Q86Y13.
PharmGKB	PA162384137.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG09840.
GeneTree	ENSGT00530000063254.
HOVERGEN	HBG051428.
InParanoid	Q86Y13.
OMA	CKYRDIL.
OrthoDB	EOG4V1700.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q86Y13.
Bgee	Q86Y13.
Genevestigator	Q86Y13.
GermOnline	ENSG00000198919. Homo sapiens.
Family and domain databases
InterPro	IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR024766. Znf_RING_H2. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KO	K10642.
Pfam	PF12678. zf-rbx1. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	36295.
SOURCE	Search...

Entry information

Entry name

DZIP3_HUMAN

Accession

Primary (citable) accession number: Q86Y13
Secondary accession number(s): B3KN01 , O75162, Q6P3R9, Q6PH82, Q86Y14, Q86Y15, Q86Y16, Q8IWI0, Q96RS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.