ID   FRAS1_HUMAN             Reviewed;        4008 AA.
AC   Q86XX4; A2RRR8; Q86UZ4; Q8N3U9; Q8NAU7; Q96JW7; Q9H6N9; Q9P228;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 2.
DT   27-MAR-2024, entry version 182.
DE   RecName: Full=Extracellular matrix organizing protein FRAS1 {ECO:0000250|UniProtKB:Q80T14};
DE   AltName: Full=Fraser syndrome 1 protein {ECO:0000303|PubMed:12766769};
DE   Flags: Precursor;
GN   Name=FRAS1 {ECO:0000312|HGNC:HGNC:19185}; Synonyms=KIAA1500;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP   INVOLVEMENT IN FRASRS1.
RX   PubMed=12766769; DOI=10.1038/ng1142;
RA   McGregor L.K., Makela V., Darling S.M., Vrontou S., Chalepakis G.,
RA   Roberts C., Smart N., Rutland P., Prescott N., Hopkins J., Bentley E.,
RA   Shaw A., Roberts E., Mueller R., Jadeja S., Philip N., Nelson J.,
RA   Francannet C., Perez-Aytes A., Megarbane A., Kerr B., Wainwright B.,
RA   Woolf A.S., Winter R.M., Scambler P.J.;
RT   "Fraser syndrome and mouse blebbed phenotype caused by mutations in
RT   FRAS1/Fras1 encoding a putative extracellular matrix protein.";
RL   Nat. Genet. 34:203-208(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 159-4008 (ISOFORM 6).
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 193-4008 (ISOFORM 4), AND
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1560-1953 (ISOFORM 1).
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1773-4008 (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=10819331; DOI=10.1093/dnares/7.2.143;
RA   Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XVII. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 7:143-150(2000).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1773-4008 (ISOFORM 5).
RC   TISSUE=Amygdala;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [9]
RP   INVOLVEMENT IN FRASRS1.
RX   PubMed=23473829; DOI=10.1016/j.gene.2013.02.031;
RA   Hoefele J., Wilhelm C., Schiesser M., Mack R., Heinrich U., Weber L.T.,
RA   Biskup S., Daumer-Haas C., Klein H.G., Rost I.;
RT   "Expanding the mutation spectrum for Fraser syndrome: identification of a
RT   novel heterozygous deletion in FRAS1.";
RL   Gene 520:194-197(2013).
CC   -!- FUNCTION: Involved in extracellular matrix organization (By
CC       similarity). Required for the regulation of epidermal-basement membrane
CC       adhesion responsible for proper organogenesis during embryonic
CC       development (By similarity). Involved in brain organization and
CC       function (By similarity). {ECO:0000250|UniProtKB:Q80T14}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q80T14};
CC       Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q80T14};
CC       Extracellular side {ECO:0000250|UniProtKB:Q80T14}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=5;
CC       Name=1;
CC         IsoId=Q86XX4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q86XX4-2; Sequence=VSP_011293, VSP_011294, VSP_011295,
CC                                  VSP_046232, VSP_011299;
CC       Name=4;
CC         IsoId=Q86XX4-4; Sequence=VSP_011287, VSP_011288;
CC       Name=5;
CC         IsoId=Q86XX4-5; Sequence=VSP_011291, VSP_011292;
CC       Name=6;
CC         IsoId=Q86XX4-6; Sequence=VSP_011289, VSP_011290;
CC   -!- TISSUE SPECIFICITY: Expressed in many adult tissues, with highest
CC       levels in kidney, pancreas and thalamus. Relatively high expression was
CC       also detected in fetal kidney and heart. {ECO:0000269|PubMed:12766769}.
CC   -!- DOMAIN: The Calx-beta domains bind calcium with high affinity and
CC       undergo a major conformational shift upon binding. {ECO:0000250}.
CC   -!- DISEASE: Fraser syndrome 1 (FRASRS1) [MIM:219000]: A form of Fraser
CC       syndrome, an autosomal recessive disorder characterized by
CC       cryptophthalmos, cutaneous syndactyly, and urogenital abnormalities
CC       including renal agenesis or hypoplasia. Additional features include
CC       abnormalities of the larynx, ear malformations, and facial
CC       abnormalities. {ECO:0000269|PubMed:12766769,
CC       ECO:0000269|PubMed:23473829}. Note=The disease is caused by variants
CC       affecting the gene represented in this entry.
CC   -!- SIMILARITY: Belongs to the FRAS1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB15216.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AJ512501; CAD54734.1; -; Genomic_DNA.
DR   EMBL; AC093652; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC093886; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC093897; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC104808; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC131945; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC052281; AAH52281.1; -; mRNA.
DR   EMBL; BC064487; AAH64487.1; -; mRNA.
DR   EMBL; BC131820; AAI31821.1; -; mRNA.
DR   EMBL; AK025684; BAB15216.1; ALT_FRAME; mRNA.
DR   EMBL; AK027833; BAB55399.1; -; mRNA.
DR   EMBL; AB040933; BAA96024.2; -; mRNA.
DR   EMBL; AL831853; CAD38554.1; -; mRNA.
DR   CCDS; CCDS54771.1; -. [Q86XX4-2]
DR   CCDS; CCDS54772.1; -. [Q86XX4-5]
DR   RefSeq; NP_001159605.1; NM_001166133.1. [Q86XX4-5]
DR   RefSeq; NP_079350.5; NM_025074.6. [Q86XX4-2]
DR   SMR; Q86XX4; -.
DR   BioGRID; 123137; 138.
DR   IntAct; Q86XX4; 31.
DR   MINT; Q86XX4; -.
DR   STRING; 9606.ENSP00000422834; -.
DR   GlyCosmos; Q86XX4; 19 sites, 2 glycans.
DR   GlyGen; Q86XX4; 20 sites, 3 O-linked glycans (3 sites).
DR   iPTMnet; Q86XX4; -.
DR   PhosphoSitePlus; Q86XX4; -.
DR   SwissPalm; Q86XX4; -.
DR   BioMuta; FRAS1; -.
DR   DMDM; 476007832; -.
DR   EPD; Q86XX4; -.
DR   jPOST; Q86XX4; -.
DR   MassIVE; Q86XX4; -.
DR   MaxQB; Q86XX4; -.
DR   PaxDb; 9606-ENSP00000326330; -.
DR   PeptideAtlas; Q86XX4; -.
DR   ProteomicsDB; 482; -.
DR   ProteomicsDB; 70338; -. [Q86XX4-1]
DR   ProteomicsDB; 70339; -. [Q86XX4-2]
DR   ProteomicsDB; 70340; -. [Q86XX4-4]
DR   ProteomicsDB; 70341; -. [Q86XX4-5]
DR   ProteomicsDB; 70342; -. [Q86XX4-6]
DR   Pumba; Q86XX4; -.
DR   Antibodypedia; 2470; 46 antibodies from 10 providers.
DR   DNASU; 80144; -.
DR   Ensembl; ENST00000325942.11; ENSP00000326330.6; ENSG00000138759.20. [Q86XX4-5]
DR   Ensembl; ENST00000502446.6; ENSP00000423645.2; ENSG00000138759.20. [Q86XX4-4]
DR   Ensembl; ENST00000508900.2; ENSP00000423809.2; ENSG00000138759.20. [Q86XX4-6]
DR   Ensembl; ENST00000512123.4; ENSP00000422834.2; ENSG00000138759.20. [Q86XX4-2]
DR   GeneID; 80144; -.
DR   KEGG; hsa:80144; -.
DR   MANE-Select; ENST00000512123.4; ENSP00000422834.2; NM_025074.7; NP_079350.5. [Q86XX4-2]
DR   UCSC; uc003hkw.4; human. [Q86XX4-1]
DR   AGR; HGNC:19185; -.
DR   CTD; 80144; -.
DR   DisGeNET; 80144; -.
DR   GeneCards; FRAS1; -.
DR   HGNC; HGNC:19185; FRAS1.
DR   HPA; ENSG00000138759; Tissue enhanced (thyroid).
DR   MalaCards; FRAS1; -.
DR   MIM; 219000; phenotype.
DR   MIM; 607830; gene.
DR   neXtProt; NX_Q86XX4; -.
DR   OpenTargets; ENSG00000138759; -.
DR   Orphanet; 2052; Fraser syndrome.
DR   Orphanet; 93100; Renal agenesis, unilateral.
DR   PharmGKB; PA134980133; -.
DR   VEuPathDB; HostDB:ENSG00000138759; -.
DR   eggNOG; KOG1216; Eukaryota.
DR   eggNOG; KOG3525; Eukaryota.
DR   eggNOG; KOG3597; Eukaryota.
DR   GeneTree; ENSGT00940000162130; -.
DR   HOGENOM; CLU_000898_0_0_1; -.
DR   InParanoid; Q86XX4; -.
DR   OMA; NSGMRVQ; -.
DR   OrthoDB; 5470912at2759; -.
DR   PathwayCommons; Q86XX4; -.
DR   SignaLink; Q86XX4; -.
DR   BioGRID-ORCS; 80144; 9 hits in 1148 CRISPR screens.
DR   ChiTaRS; FRAS1; human.
DR   GeneWiki; FRAS1; -.
DR   GenomeRNAi; 80144; -.
DR   Pharos; Q86XX4; Tbio.
DR   PRO; PR:Q86XX4; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; Q86XX4; Protein.
DR   Bgee; ENSG00000138759; Expressed in germinal epithelium of ovary and 144 other cell types or tissues.
DR   ExpressionAtlas; Q86XX4; baseline and differential.
DR   GO; GO:0005604; C:basement membrane; ISS:BHF-UCL.
DR   GO; GO:0062023; C:collagen-containing extracellular matrix; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005201; F:extracellular matrix structural constituent; ISS:BHF-UCL.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR   GO; GO:0007154; P:cell communication; IEA:InterPro.
DR   GO; GO:0030326; P:embryonic limb morphogenesis; IEA:Ensembl.
DR   GO; GO:0003338; P:metanephros morphogenesis; IEA:Ensembl.
DR   GO; GO:0002009; P:morphogenesis of an epithelium; IEA:Ensembl.
DR   GO; GO:0015031; P:protein transport; IEA:Ensembl.
DR   GO; GO:0060021; P:roof of mouth development; IEA:Ensembl.
DR   GO; GO:0043588; P:skin development; IEA:Ensembl.
DR   CDD; cd00064; FU; 12.
DR   Gene3D; 2.60.40.2030; -; 5.
DR   Gene3D; 6.20.200.20; -; 4.
DR   Gene3D; 2.10.70.10; Complement Module, domain 1; 2.
DR   InterPro; IPR038081; CalX-like_sf.
DR   InterPro; IPR003644; Calx_beta.
DR   InterPro; IPR039005; CSPG_rpt.
DR   InterPro; IPR000742; EGF-like_dom.
DR   InterPro; IPR006212; Furin_repeat.
DR   InterPro; IPR009030; Growth_fac_rcpt_cys_sf.
DR   InterPro; IPR001007; VWF_dom.
DR   PANTHER; PTHR45739:SF1; EXTRACELLULAR MATRIX ORGANIZING PROTEIN FRAS1; 1.
DR   PANTHER; PTHR45739; MATRIX PROTEIN, PUTATIVE-RELATED; 1.
DR   Pfam; PF16184; Cadherin_3; 12.
DR   Pfam; PF03160; Calx-beta; 5.
DR   Pfam; PF00093; VWC; 4.
DR   SMART; SM00237; Calx_beta; 5.
DR   SMART; SM00181; EGF; 9.
DR   SMART; SM00261; FU; 14.
DR   SMART; SM00214; VWC; 6.
DR   SMART; SM00215; VWC_out; 3.
DR   SUPFAM; SSF141072; CalX-like; 5.
DR   SUPFAM; SSF57603; FnI-like domain; 6.
DR   SUPFAM; SSF57184; Growth factor receptor domain; 6.
DR   PROSITE; PS51854; CSPG; 12.
DR   PROSITE; PS01208; VWFC_1; 6.
DR   PROSITE; PS50184; VWFC_2; 6.
DR   Genevisible; Q86XX4; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Calcium; Cell membrane; Glycoprotein; Membrane;
KW   Metal-binding; Phosphoprotein; Reference proteome; Repeat; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..4008
FT                   /note="Extracellular matrix organizing protein FRAS1"
FT                   /id="PRO_0000010120"
FT   TOPO_DOM        27..3901
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        3902..3922
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        3923..4008
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          27..88
FT                   /note="VWFC 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   DOMAIN          93..153
FT                   /note="VWFC 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   DOMAIN          157..217
FT                   /note="VWFC 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   DOMAIN          219..279
FT                   /note="VWFC 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   DOMAIN          283..343
FT                   /note="VWFC 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   DOMAIN          347..417
FT                   /note="VWFC 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00220"
FT   REPEAT          409..460
FT                   /note="FU 1"
FT   REPEAT          462..505
FT                   /note="FU 2"
FT   REPEAT          507..553
FT                   /note="FU 3"
FT   REPEAT          555..599
FT                   /note="FU 4"
FT   REPEAT          602..647
FT                   /note="FU 5"
FT   REPEAT          649..705
FT                   /note="FU 6"
FT   REPEAT          708..753
FT                   /note="FU 7"
FT   REPEAT          755..800
FT                   /note="FU 8"
FT   REPEAT          803..852
FT                   /note="FU 9"
FT   REPEAT          854..900
FT                   /note="FU 10"
FT   REPEAT          903..948
FT                   /note="FU 11"
FT   REPEAT          952..997
FT                   /note="FU 12"
FT   REPEAT          999..1042
FT                   /note="FU 13"
FT   REPEAT          1046..1089
FT                   /note="FU 14"
FT   REPEAT          1102..1197
FT                   /note="CSPG 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1217..1308
FT                   /note="CSPG 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1329..1438
FT                   /note="CSPG 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1463..1559
FT                   /note="CSPG 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1595..1689
FT                   /note="CSPG 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1710..1810
FT                   /note="CSPG 6"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1833..1936
FT                   /note="CSPG 7"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          1957..2057
FT                   /note="CSPG 8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          2078..2177
FT                   /note="CSPG 9"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          2199..2291
FT                   /note="CSPG 10"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          2311..2404
FT                   /note="CSPG 11"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   REPEAT          2439..2536
FT                   /note="CSPG 12"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01201"
FT   DOMAIN          2543..2646
FT                   /note="Calx-beta 1"
FT   DOMAIN          2659..2770
FT                   /note="Calx-beta 2"
FT   DOMAIN          2784..2890
FT                   /note="Calx-beta 3"
FT   DOMAIN          2905..3007
FT                   /note="Calx-beta 4"
FT   DOMAIN          3025..3129
FT                   /note="Calx-beta 5"
FT   MOD_RES         344
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19690332"
FT   CARBOHYD        361
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        728
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1093
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1108
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1504
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1777
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1948
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1978
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2563
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2664
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2682
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2908
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        2985
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3070
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3218
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3676
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        3875
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         713..737
FT                   /note="ACHQSCFRCAGKSPHNCTDCGPSHV -> GQNLDFCQNLEVISAVCLGISST
FT                   EN (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_011287"
FT   VAR_SEQ         738..4008
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_011288"
FT   VAR_SEQ         808..809
FT                   /note="DC -> GE (in isoform 6)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_011289"
FT   VAR_SEQ         810..4008
FT                   /note="Missing (in isoform 6)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_011290"
FT   VAR_SEQ         1953..1976
FT                   /note="RKNDEPPRMTLQPLRVQLSSGVVI -> VKTLEVGKVEPLTTIFHTIRELSL
FT                   (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:15489334,
FT                   ECO:0000303|PubMed:17974005"
FT                   /id="VSP_011291"
FT   VAR_SEQ         1977..4008
FT                   /note="Missing (in isoform 5)"
FT                   /evidence="ECO:0000303|PubMed:15489334,
FT                   ECO:0000303|PubMed:17974005"
FT                   /id="VSP_011292"
FT   VAR_SEQ         2156..2157
FT                   /note="QP -> HV (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10819331"
FT                   /id="VSP_011293"
FT   VAR_SEQ         2194
FT                   /note="S -> L (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10819331"
FT                   /id="VSP_011294"
FT   VAR_SEQ         2598
FT                   /note="S -> SSSRV (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10819331"
FT                   /id="VSP_011295"
FT   VAR_SEQ         2717..2718
FT                   /note="LF -> SL (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10819331"
FT                   /id="VSP_046232"
FT   VAR_SEQ         3386..3387
FT                   /note="LA -> IS (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:10819331"
FT                   /id="VSP_011299"
FT   VARIANT         32
FT                   /note="D -> G (in dbSNP:rs4859905)"
FT                   /id="VAR_069150"
FT   VARIANT         54
FT                   /note="D -> H (in dbSNP:rs17003071)"
FT                   /id="VAR_055807"
FT   VARIANT         209
FT                   /note="P -> S (in dbSNP:rs7699637)"
FT                   /id="VAR_055808"
FT   VARIANT         228
FT                   /note="Y -> H (in dbSNP:rs7682296)"
FT                   /id="VAR_055809"
FT   VARIANT         243
FT                   /note="I -> V (in dbSNP:rs6848030)"
FT                   /id="VAR_055810"
FT   VARIANT         429
FT                   /note="S -> Y (in dbSNP:rs6838959)"
FT                   /id="VAR_055811"
FT   VARIANT         466
FT                   /note="L -> I (in dbSNP:rs12504081)"
FT                   /id="VAR_055812"
FT   VARIANT         590
FT                   /note="M -> T (in dbSNP:rs35030041)"
FT                   /id="VAR_055813"
FT   VARIANT         687
FT                   /note="D -> G (in dbSNP:rs345513)"
FT                   /id="VAR_069151"
FT   VARIANT         710
FT                   /note="I -> L (in dbSNP:rs345512)"
FT                   /id="VAR_069152"
FT   VARIANT         817
FT                   /note="A -> V (in dbSNP:rs6835769)"
FT                   /id="VAR_055814"
FT   VARIANT         954
FT                   /note="T -> M (in dbSNP:rs17003166)"
FT                   /id="VAR_055815"
FT   VARIANT         1023
FT                   /note="G -> E (in dbSNP:rs17459809)"
FT                   /id="VAR_055816"
FT   VARIANT         1136
FT                   /note="E -> K (in dbSNP:rs12512164)"
FT                   /id="VAR_055817"
FT   VARIANT         1626
FT                   /note="A -> V (in dbSNP:rs17003213)"
FT                   /id="VAR_055818"
FT   VARIANT         2545
FT                   /note="D -> N (in dbSNP:rs4388111)"
FT                   /id="VAR_069153"
SQ   SEQUENCE   4008 AA;  443214 MW;  79972020FA59C3CD CRC64;
     MGVLKVWLGL ALALAEFAVL PHHSEGACVY QDSLLADATI WKPDSCQSCR CHGDIVICKP
     AVCRNPQCAF EKGEVLQIAA NQCCPECVLR TPGSCHHEKK IHEHGTEWAS SPCSVCSCNH
     GEVRCTPQPC PPLSCGHQEL AFIPEGSCCP VCVGLGKPCS YEGHVFQDGE DWRLSRCAKC
     LCRNGVAQCF TAQCQPLFCN QDETVVRVPG KCCPQCSARS CSAAGQVYEH GEQWSENACT
     TCICDRGEVR CHKQACLPLR CGKGQSRARR HGQCCEECVS PAGSCSYDGV VRYQDEMWKG
     SACEFCMCDH GQVTCQTGEC AKVECARDEE LIHLDGKCCP ECISRNGYCV YEETGEFMSS
     NASEVKRIPE GEKWEDGPCK VCECRGAQVT CYEPSCPPCP VGTLALEVKG QCCPDCTSVH
     CHPDCLTCSQ SPDHCDLCQD PTKLLQNGWC VHSCGLGFYQ AGSLCLACQP QCSTCTSGLE
     CSSCQPPLLM RHGQCVPTCG DGFYQDRHSC AVCHESCAGC WGPTEKHCLA CRDPLHVLRD
     GGCESSCGKG FYNRQGTCSA CDQSCDSCGP SSPRCLTCTE KTVLHDGKCM SECPGGYYAD
     ATGRCKVCHN SCASCSGPTP SHCTACSPPK ALRQGHCLPR CGEGFYSDHG VCKACHSSCL
     ACMGPAPSHC TGCKKPEEGL QVEQLSDVGI PSGECLAQCR AHFYLESTGI CEACHQSCFR
     CAGKSPHNCT DCGPSHVLLD GQCLSQCPDG YFHQEGSCTE CHPTCRQCHG PLESDCISCY
     PHISLTNGNC RTSCREEQFL NLVGYCADCH HLCQHCAADL HNTGSICLRC QNAHYLLLGD
     HCVPDCPSGY YAERGACKKC HSSCRTCQGR GPFSCSSCDT NLVLSHTGTC STTCFPGHYL
     DDNHVCQPCN THCGSCDSQA SCTSCRDPNK VLLFGECQYE SCAPQYYLDF STNTCKECDW
     SCSACSGPLK TDCLQCMDGY VLQDGACVEQ CLSSFYQDSG LCKNCDSYCL QCQGPHECTR
     CKGPFLLLEA QCVQECGKGY FADHAKHKCT ACPQGCLQCS HRDRCHLCDH GFFLKSGLCV
     YNCVPGFSVH TSNETCSGKI HTPSLHVNGS LILPIGSIKP LDFSLLNVQD QEGRVEDLLF
     HVVSTPTNGQ LVLSRNGKEV QLDKAGRFSW KDVNEKKVRF VHSKEKLRKG YLFLKISDQQ
     FFSEPQLINI QAFSTQAPYV LRNEVLHISR GERATITTQM LDIRDDDNPQ DVVIEIIDPP
     LHGQLLQTLQ SPATPIYQFQ LDELSRGLLH YAHDGSDSTS DVAVLQANDG HSFHNILFQV
     KTVPQNDRGL QLVANSMVWV PEGGMLQITN RILQAEAPGA SAEEIIYKIT QDYPQFGEVV
     LLVNMPADSP ADEGQHLPDG RTATPTSTFT QQDINEGIVW YRHSGAPAQS DSFRFEVSSA
     SNAQTRLESH MFNIAILPQT PEAPKVSLEA SLHMTAREDG LTVIQPHSLS FINSEKPSGK
     IVYNITLPLH PNQGIIEHRD HPHSPIRYFT QEDINQGKVM YRPPPAAPHL QELMAFSFAG
     LPESVKFHFT VSDGEHTSPE MVLTIHLLPS DQQLPVFQVT APRLAVSPGG STSVGLQVVV
     RDAETAPKEL FFELRRPPQH GVLLKHTAEF RRPMATGDTF TYEDVEKNAL QYIHDGSSTR
     EDSMEISVTD GLTVTMLEVR VEVSLSEDRG PRLAAGSSLS ITVASKSTAI ITRSHLAYVD
     DSSPDPEIWI QLNYLPSYGT LLRISGSEVE ELSEVSNFTM EDINNKKIRY SAVFETDGHL
     VTDSFYFSVS DMDHNHLDNQ IFTIMITPAE NPPPVIAFAD LITVDEGGRA PLSFHHFFAT
     DDDDNLQRDA IIKLSALPKY GCIENTGTGD RFGPETASDL EASFPIQDVL ENYIYYFQSV
     HESIEPTHDI FSFYVSDGTS RSEIHSINIT IERKNDEPPR MTLQPLRVQL SSGVVISNSS
     LSLQDLDTPD NELIFVLTKK PDHGHVLWRQ TASEPLENGR VLVQGSTFTY QDILAGLVGY
     VPSVPGMVVD EFQFSLTDGL HVDTGRMKIY TELPASDTPH LAINQGLQLS AGSVARITEQ
     HLKVTDIDSD DHQVMYIMKE DPGAGRLQMM KHGNLEQISI KGPIRSFTQA DISQGQPEYS
     HGTGEPGGSF AFKFDVVDGE GNRLIDKSFS ISISEDKSPP VITTNKGLVL DENSVKKITT
     LQLSATDQDS GPTELIYRIT RQPQLGHLEH AASPGIQISS FTQADLTSRN VQYVHSSEAE
     KHSDAFSFTL SDGVSEVTQT FHITLHPVDD SLPVVQNLGM RVQEGMRKTI TEFELKAVDA
     DTEAESVTFT IVQPPRHGTI ERTSNGQHFH LTSTFTMKDI YQNRVSYSHD GSNSLKDRFT
     FTVSDGTNPF FIIEEGGKEI MTAAPQPFRV DILPVDDGTP RIVTNLGLQW LEYMDGKATN
     LITKKELLTM DPDTEDAQLV YEITTGPKHG FVENKLQPGR AAATFTQEDV NLGLIRYVLH
     KEKIREMMDS FQFLVKDSKP NVVSDNVFHI QWSLISFKYT SYNVSEKAGS VSVTVQRTGN
     LNQYAIVLCR TEQGTASSSS QPGQQDYVEY AGQVQFDERE DTKSCTIVIN DDDVFENVES
     FTVELSMPAY ALLGEFTQAK VIINDTEDEP TLEFDKKIYW VNESAGFLFA PIERKGDASS
     IVSAICYTVP KSAMGSLFYA LESGSDFKSR GMSAASRVIF GPGVTMSTCD VMLIDDSEYE
     EEEEFEIALA DASDNARIGR VATAKVLISG PNDASTVSLG NTAFTVSEDA GTVKIPVIRH
     GTDLSTFASV WCATRPSDPA SATPGVDYVP SSRKVEFGPG VIEQYCTLTI LDDTQYPVIE
     GLETFVVFLS SAQGAELTKP FQAVIAINDT FQDVPSMQFA KDLLLVKEKE GVLHVPITRS
     GDLSYESSVR CYTQSHSAQV MEDFEERQNA DSSRITFLKG DKVKNCTVYI HDDSMFEPEE
     QFRVYLGLPL GNHWSGARIG KNNMATITIS NDEDAPTIEF EEAAYQVREP AGPDAIAILN
     IKVIRRGDQN RTSKVRCSTR DGSAQSGVDY YPKSRVLKFS PGVDHIFFKV EILSNEDREW
     HESFSLVLGP DDPVEAVLGD VTTATVTILD QEAAGSLILP APPIVVTLAD YDHVEEVTKE
     GVKKSPSPGY PLVCVTPCDP HFPRYAVMKE RCSEAGINQT SVQFSWEVAA PTDGNGARSP
     FETITDNTPF TSVNHMVLDS IYFSRRFHVR CVAKAVDKVG HVGTPLRSNI VTIGTDSAIC
     HTPVVAGTSR GFQAQSFIAT LKYLDVKHKE HPNRIHISVQ IPHQDGMLPL ISTMPLHNLH
     FLLSESIYRH QHVCSNLVTT YDLRGLAEAG FLDDVVYDST ALGPGYDRPF QFDPSVREPK
     TIQLYKHLNL KSCVWTFDAY YDMTELIDVC GGSVTADFQV RDSAQSFLTV HVPLYVSYIY
     VTAPRGWASL EHHTEMEFSF FYDTVLWRTG IQTDSVLSAR LQIIRIYIRE DGRLVIEFKT
     HAKFRGQFVM EHHTLPEVKS FVLTPDHLGG IEFDLQLLWS AQTFDSPHQL WRATSSYNRK
     DYSGEYTIYL IPCTVQPTQP WVDPGEKPLA CTAHAPERFL IPIAFQQTNR PVPVVYSLNT
     EFQLCNNEKV FLMDPNTSDM SLAEMDYKGA FSKGQILYGR VLWNPEQNLN SAYKLQLEKV
     YLCTGKDGYV PFFDPTGTIY NEGPQYGCIQ PNKHLKHRFL LLDRNQPEVT DKYFHDVPFE
     AHFASELPDF HVVSNMPGVD GFTLKVDALY KVEAGHQWYL QVIYIIGPDT ISGPRVQRSL
     TAPLRRNRRD LVEPDGQLIL DDSLIYDNEG DQVKNGTNMK SLNLEMQELA VAASLSQTGA
     SIGSALAAIM LLLLVFLVAC FINRKCQKQR KKKPAEDILE EYPLNTKVEV PKRHPDRVEK
     NVNRHYCTVR NVNILSEPEA AYTFKGAKVK RLNLEVRVHN NLQDGTEV
//