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Protein

E3 ubiquitin-protein ligase TRIM50

Gene

TRIM50

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 5742RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 12542B box-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi6.3.2.19. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM50 (EC:6.3.2.-)
Alternative name(s):
Tripartite motif-containing protein 50
Gene namesi
Name:TRIM50
Synonyms:TRIM50A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:19017. TRIM50.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA38778.

Polymorphism and mutation databases

DMDMi56404881.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487E3 ubiquitin-protein ligase TRIM50PRO_0000056274Add
BLAST

Post-translational modificationi

Auto-ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ86XT4.
PRIDEiQ86XT4.

PTM databases

iPTMnetiQ86XT4.
PhosphoSiteiQ86XT4.

Expressioni

Gene expression databases

BgeeiQ86XT4.
CleanExiHS_TRIM50.
ExpressionAtlasiQ86XT4. baseline and differential.
GenevisibleiQ86XT4. HS.

Organism-specific databases

HPAiHPA019862.

Interactioni

Subunit structurei

Can form dimers and trimers. Interacts with several E2 ubiquitin-conjugating enzymes, including UBE2L6, UBE2E1, UBE2E3. No interaction with UBE2H.1 Publication

Protein-protein interaction databases

BioGridi126435. 11 interactions.
IntActiQ86XT4. 1 interaction.
STRINGi9606.ENSP00000327994.

Structurei

3D structure databases

ProteinModelPortaliQ86XT4.
SMRiQ86XT4. Positions 16-56, 89-230, 283-470.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini276 – 475200B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili125 – 16945Sequence analysisAdd
BLAST
Coiled coili204 – 23532Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B box-type zinc finger.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri16 – 5742RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri84 – 12542B box-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
HOGENOMiHOG000234133.
HOVERGENiHBG106596.
InParanoidiQ86XT4.
KOiK12024.
OrthoDBiEOG74R1QB.
PhylomeDBiQ86XT4.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q86XT4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWQVSLLEL EDWLQCPICL EVFKEPLMLQ CGHSYCKGCL VSLSCHLDAE
60 70 80 90 100
LRCPVCRQAV DGSSSLPNVS LARVIEALRL PGDPEPKVCV HHRNPLSLFC
110 120 130 140 150
EKDQELICGL CGLLGSHQHH PVTPVSTVYS RMKEELAALI SELKQEQKKV
160 170 180 190 200
DELIAKLVNN RTRIVNESDV FSWVIRREFQ ELHHLVDEEK ARCLEGIGGH
210 220 230 240 250
TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHKF IRKFHSMASR
260 270 280 290 300
AEMPQARPLE GAFSPISFKP GLHQADIKLT VWKRLFRKVL PAPEPLKLDP
310 320 330 340 350
ATAHPLLELS KGNTVVQCGL LAQRRASQPE RFDYSTCVLA SRGFSCGRHY
360 370 380 390 400
WEVVVGSKSD WRLGVIKGTA SRKGKLNRSP EHGVWLIGLK EGRVYEAFAC
410 420 430 440 450
PRVPLPVAGH PHRIGLYLHY EQGELTFFDA DRPDDLRPLY TFQADFQGKL
460 470 480
YPILDTCWHE RGSNSLPMVL PPPSGPGPLS PEQPTKL
Note: No experimental confirmation available.
Length:487
Mass (Da):54,774
Last modified:June 1, 2003 - v1
Checksum:iDB06FD97C10D723C
GO
Isoform Beta (identifier: Q86XT4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-243: Missing.

Show »
Length:486
Mass (Da):54,646
Checksum:iB3082598C74EE194
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81L → P.
Corresponds to variant rs6980124 [ dbSNP | Ensembl ].
VAR_020491

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei243 – 2431Missing in isoform Beta. CuratedVSP_012088

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081948 mRNA. Translation: AAL91071.1.
AY081949 mRNA. Translation: AAL91072.1.
AC073841 Genomic DNA. No translation available.
CCDSiCCDS34654.1. [Q86XT4-1]
RefSeqiNP_001268379.1. NM_001281450.1.
NP_001268380.1. NM_001281451.1.
NP_835226.2. NM_178125.3.
UniGeneiHs.647053.

Genome annotation databases

EnsembliENST00000333149; ENSP00000327994; ENSG00000146755.
ENST00000453152; ENSP00000413875; ENSG00000146755.
GeneIDi135892.
KEGGihsa:135892.
UCSCiuc032zrf.2. human. [Q86XT4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY081948 mRNA. Translation: AAL91071.1.
AY081949 mRNA. Translation: AAL91072.1.
AC073841 Genomic DNA. No translation available.
CCDSiCCDS34654.1. [Q86XT4-1]
RefSeqiNP_001268379.1. NM_001281450.1.
NP_001268380.1. NM_001281451.1.
NP_835226.2. NM_178125.3.
UniGeneiHs.647053.

3D structure databases

ProteinModelPortaliQ86XT4.
SMRiQ86XT4. Positions 16-56, 89-230, 283-470.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126435. 11 interactions.
IntActiQ86XT4. 1 interaction.
STRINGi9606.ENSP00000327994.

PTM databases

iPTMnetiQ86XT4.
PhosphoSiteiQ86XT4.

Polymorphism and mutation databases

DMDMi56404881.

Proteomic databases

PaxDbiQ86XT4.
PRIDEiQ86XT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333149; ENSP00000327994; ENSG00000146755.
ENST00000453152; ENSP00000413875; ENSG00000146755.
GeneIDi135892.
KEGGihsa:135892.
UCSCiuc032zrf.2. human. [Q86XT4-1]

Organism-specific databases

CTDi135892.
GeneCardsiTRIM50.
HGNCiHGNC:19017. TRIM50.
HPAiHPA019862.
MIMi612548. gene.
neXtProtiNX_Q86XT4.
PharmGKBiPA38778.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ITFB. Eukaryota.
ENOG410XWXM. LUCA.
HOGENOMiHOG000234133.
HOVERGENiHBG106596.
InParanoidiQ86XT4.
KOiK12024.
OrthoDBiEOG74R1QB.
PhylomeDBiQ86XT4.
TreeFamiTF342569.

Enzyme and pathway databases

BRENDAi6.3.2.19. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii135892.
PROiQ86XT4.
SOURCEiSearch...

Gene expression databases

BgeeiQ86XT4.
CleanExiHS_TRIM50.
ExpressionAtlasiQ86XT4. baseline and differential.
GenevisibleiQ86XT4. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00336. BBOX. 1 hit.
SM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS ALPHA AND BETA), FUNCTION, SUBCELLULAR LOCATION, AUTOUBIQUITINATION, DIMERIZATION, TRIMERIZATION, INTERACTION WITH UBE2L6; UBE2E1 AND UBE2E3.
  2. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRI50_HUMAN
AccessioniPrimary (citable) accession number: Q86XT4
Secondary accession number(s): Q86XT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.