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Q86XS8 (GOLI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase RNF130

EC=6.3.2.-
Alternative name(s):
Goliath homolog
Short name=H-Goliath
RING finger protein 130
Gene names
Name:RNF130
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role during the programmed cell death of hematopoietic cells By similarity. Acts as an E3 ubiquitin-protein ligase. Ref.4

Pathway

Protein modification; protein ubiquitination. Ref.4

Subcellular location

Membrane; Single-pass type I membrane protein Potential. Cytoplasm Ref.4.

Tissue specificity

Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts. Ref.4

Post-translational modification

In vivo measurements suggest this protein is glycosylated (Ref.5). In contrast, in vitro experiments failed to detect glycosylation (Ref.4). Ref.4

Sequence similarities

Contains 1 PA (protease associated) domain.

Contains 1 RING-type zinc finger.

Sequence caution

The sequence AAF67007.1 differs from that shown. Reason: Frameshift at positions 110 and 116.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 419392E3 ubiquitin-protein ligase RNF130
PRO_0000030718

Regions

Topological domain28 – 194167Extracellular Potential
Transmembrane195 – 21723Helical; Potential
Topological domain218 – 419202Cytoplasmic Potential
Domain105 – 17672PA
Zinc finger264 – 30542RING-type

Amino acid modifications

Modified residue3411Phosphoserine Ref.6
Glycosylation291N-linked (GlcNAc...) Potential
Glycosylation401N-linked (GlcNAc...) Ref.5
Glycosylation1121N-linked (GlcNAc...) Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation1891N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1141T → A in AAH65244. Ref.2
Sequence conflict1341N → T in AAF67007. Ref.3
Sequence conflict1411P → N in AAH82267. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q86XS8 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 74E8D987334F3B8E

FASTA41946,405
        10         20         30         40         50         60 
MSCAGRAGPA RLAALALLTC SLWPARADNA SQEYYTALIN VTVQEPGRGA PLTFRIDRGR 

        70         80         90        100        110        120 
YGLDSPKAEV RGQVLAPLPL HGVADHLGCD PQTRFFVPPN IKQWIALLQR GNCTFKEKIS 

       130        140        150        160        170        180 
RAAFHNAVAV VIYNNKSKEE PVTMTHPGTG DIIAVMITEL RGKDILSYLE KNISVQMTIA 

       190        200        210        220        230        240 
VGTRMPPKNF SRGSLVFVSI SFIVLMIISS AWLIFYFIQK IRYTNARDRN QRRLGDAAKK 

       250        260        270        280        290        300 
AISKLTTRTV KKGDKETDPD FDHCAVCIES YKQNDVVRIL PCKHVFHKSC VDPWLSEHCT 

       310        320        330        340        350        360 
CPMCKLNILK ALGIVPNLPC TDNVAFDMER LTRTQAVNRR SALGDLAGDN SLGLEPLRTS 

       370        380        390        400        410 
GISPLPQDGE LTPRTGEINI AVTKEWFIIA SFGLLSALTL CYMIIRATAS LNANEVEWF 

« Hide

References

« Hide 'large scale' references
[1]"Goliath, a ring-H2 mitochondrial protein, regulated by luteinizing hormone/human chorionic gonadotropin in rat Leydig cells."
Guais A., Solhonne B., Melaine N., Guellaeen G., Bulle F.
Biol. Reprod. 70:204-213(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye, Lung, Mammary gland and Skin.
[3]"A novel gene expressed in the human adrenal gland."
Gu J., Fu S., Ren S., Jin W., Gu Y., Huang Q., Dong H., Yu Y., Fu G., Wang Y., Chen Z., Han Z.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 103-419.
Tissue: Adrenal gland.
[4]"h-Goliath, paralog of GRAIL, is a new E3 ligase protein, expressed in human leukocytes."
Guais A., Siegrist S., Solhonne B., Jouault H., Guellaen G., Bulle F.
Gene 374:112-120(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LACK OF GLYCOSYLATION.
[5]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-40.
Tissue: Liver.
[6]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY083998 mRNA. Translation: AAM08686.1.
BC017100 mRNA. Translation: AAH17100.2.
BC065244 mRNA. Translation: AAH65244.1.
BC082267 mRNA. Translation: AAH82267.1.
BC108306 mRNA. Translation: AAI08307.1.
AF155650 mRNA. Translation: AAF67007.1. Frameshift.
RefSeqNP_060904.2. NM_018434.5.
UniGeneHs.484363.

3D structure databases

ProteinModelPortalQ86XS8.
SMRQ86XS8. Positions 27-187, 264-305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120928. 12 interactions.
IntActQ86XS8. 9 interactions.
MINTMINT-1388856.

PTM databases

PhosphoSiteQ86XS8.

Polymorphism databases

DMDM56749089.

Proteomic databases

PaxDbQ86XS8.
PRIDEQ86XS8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000520911; ENSP00000430999; ENSG00000113269.
ENST00000521389; ENSP00000430237; ENSG00000113269.
GeneID55819.
KEGGhsa:55819.
UCSCuc003mll.1. human.

Organism-specific databases

CTD55819.
GeneCardsGC05M179345.
HGNCHGNC:18280. RNF130.
HPAHPA014499.
neXtProtNX_Q86XS8.
PharmGKBPA134871556.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG302028.
HOGENOMHOG000231432.
HOVERGENHBG057659.
InParanoidQ86XS8.
KOK15701.
PhylomeDBQ86XS8.
TreeFamTF317486.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ86XS8.
BgeeQ86XS8.
CleanExHS_RNF130.
GenevestigatorQ86XS8.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR003137. Protease-assoc_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
[Graphical view]
PROSITEPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSRNF130. human.
GenomeRNAi55819.
NextBio61006.
PROQ86XS8.

Entry information

Entry nameGOLI_HUMAN
AccessionPrimary (citable) accession number: Q86XS8
Secondary accession number(s): Q32P32 expand/collapse secondary AC list , Q641P9, Q6P177, Q7L2U2, Q9P0J9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 2003
Last modified: March 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM