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Q86XS8

- GOLI_HUMAN

UniProt

Q86XS8 - GOLI_HUMAN

Protein

E3 ubiquitin-protein ligase RNF130

Gene

RNF130

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    May have a role during the programmed cell death of hematopoietic cells By similarity. Acts as an E3 ubiquitin-protein ligase.By similarity1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri264 – 30542RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. programmed cell death Source: UniProtKB

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Apoptosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase RNF130 (EC:6.3.2.-)
    Alternative name(s):
    Goliath homolog
    Short name:
    H-Goliath
    RING finger protein 130
    Gene namesi
    Name:RNF130Imported
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:18280. RNF130.

    Subcellular locationi

    Membrane Curated; Single-pass type I membrane protein Curated. Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW
    3. nucleus Source: HPA

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134871556.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 419392E3 ubiquitin-protein ligase RNF130PRO_0000030718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi40 – 401N-linked (GlcNAc...)1 Publication
    Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
    Modified residuei341 – 3411Phosphoserine1 Publication

    Post-translational modificationi

    In vivo measurements suggest this protein is glycosylated (PubMed:19159218). In contrast, in vitro experiments failed to detect glycosylation (PubMed:16549277).2 Publications

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ86XS8.
    PaxDbiQ86XS8.
    PRIDEiQ86XS8.

    PTM databases

    PhosphoSiteiQ86XS8.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts.1 Publication

    Gene expression databases

    ArrayExpressiQ86XS8.
    BgeeiQ86XS8.
    CleanExiHS_RNF130.
    GenevestigatoriQ86XS8.

    Organism-specific databases

    HPAiHPA014499.

    Interactioni

    Protein-protein interaction databases

    BioGridi120928. 12 interactions.
    IntActiQ86XS8. 9 interactions.
    MINTiMINT-1388856.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86XS8.
    SMRiQ86XS8. Positions 27-187, 264-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 194167ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini218 – 419202CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei195 – 21723HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini105 – 17672PAAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri264 – 30542RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG302028.
    HOGENOMiHOG000231432.
    HOVERGENiHBG057659.
    InParanoidiQ86XS8.
    KOiK15701.
    PhylomeDBiQ86XS8.
    TreeFamiTF317486.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR003137. Protease-assoc_domain.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF02225. PA. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86XS8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSCAGRAGPA RLAALALLTC SLWPARADNA SQEYYTALIN VTVQEPGRGA    50
    PLTFRIDRGR YGLDSPKAEV RGQVLAPLPL HGVADHLGCD PQTRFFVPPN 100
    IKQWIALLQR GNCTFKEKIS RAAFHNAVAV VIYNNKSKEE PVTMTHPGTG 150
    DIIAVMITEL RGKDILSYLE KNISVQMTIA VGTRMPPKNF SRGSLVFVSI 200
    SFIVLMIISS AWLIFYFIQK IRYTNARDRN QRRLGDAAKK AISKLTTRTV 250
    KKGDKETDPD FDHCAVCIES YKQNDVVRIL PCKHVFHKSC VDPWLSEHCT 300
    CPMCKLNILK ALGIVPNLPC TDNVAFDMER LTRTQAVNRR SALGDLAGDN 350
    SLGLEPLRTS GISPLPQDGE LTPRTGEINI AVTKEWFIIA SFGLLSALTL 400
    CYMIIRATAS LNANEVEWF 419
    Length:419
    Mass (Da):46,405
    Last modified:June 1, 2003 - v1
    Checksum:i74E8D987334F3B8E
    GO
    Isoform 2 (identifier: Q86XS8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         384-384: K → R
         385-419: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:384
    Mass (Da):42,456
    Checksum:i76C96ED33B701F28
    GO

    Sequence cautioni

    The sequence AAF67007.1 differs from that shown. Reason: Frameshift at positions 110 and 116.
    The sequence BAD93037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141T → A in AAH65244. (PubMed:15489334)Curated
    Sequence conflicti134 – 1341N → T in AAF67007. 1 PublicationCurated
    Sequence conflicti141 – 1411P → N in AAH82267. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei384 – 3841K → R in isoform 2. 1 PublicationVSP_055642
    Alternative sequencei385 – 41935Missing in isoform 2. 1 PublicationVSP_055643Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY083998 mRNA. Translation: AAM08686.1.
    AB209800 mRNA. Translation: BAD93037.1. Different initiation.
    AC010285 Genomic DNA. No translation available.
    AC026413 Genomic DNA. No translation available.
    AC122713 Genomic DNA. No translation available.
    CH471165 Genomic DNA. Translation: EAW53773.1.
    BC017100 mRNA. Translation: AAH17100.2.
    BC065244 mRNA. Translation: AAH65244.1.
    BC082267 mRNA. Translation: AAH82267.1.
    BC108306 mRNA. Translation: AAI08307.1.
    AF155650 mRNA. Translation: AAF67007.1. Frameshift.
    CCDSiCCDS4451.1.
    RefSeqiNP_060904.2. NM_018434.5.
    UniGeneiHs.484363.

    Genome annotation databases

    EnsembliENST00000261947; ENSP00000261947; ENSG00000113269. [Q86XS8-2]
    ENST00000520911; ENSP00000430999; ENSG00000113269.
    ENST00000521389; ENSP00000430237; ENSG00000113269. [Q86XS8-1]
    GeneIDi55819.
    KEGGihsa:55819.
    UCSCiuc003mll.1. human.

    Polymorphism databases

    DMDMi56749089.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY083998 mRNA. Translation: AAM08686.1 .
    AB209800 mRNA. Translation: BAD93037.1 . Different initiation.
    AC010285 Genomic DNA. No translation available.
    AC026413 Genomic DNA. No translation available.
    AC122713 Genomic DNA. No translation available.
    CH471165 Genomic DNA. Translation: EAW53773.1 .
    BC017100 mRNA. Translation: AAH17100.2 .
    BC065244 mRNA. Translation: AAH65244.1 .
    BC082267 mRNA. Translation: AAH82267.1 .
    BC108306 mRNA. Translation: AAI08307.1 .
    AF155650 mRNA. Translation: AAF67007.1 . Frameshift.
    CCDSi CCDS4451.1.
    RefSeqi NP_060904.2. NM_018434.5.
    UniGenei Hs.484363.

    3D structure databases

    ProteinModelPortali Q86XS8.
    SMRi Q86XS8. Positions 27-187, 264-305.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 120928. 12 interactions.
    IntActi Q86XS8. 9 interactions.
    MINTi MINT-1388856.

    PTM databases

    PhosphoSitei Q86XS8.

    Polymorphism databases

    DMDMi 56749089.

    Proteomic databases

    MaxQBi Q86XS8.
    PaxDbi Q86XS8.
    PRIDEi Q86XS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000261947 ; ENSP00000261947 ; ENSG00000113269 . [Q86XS8-2 ]
    ENST00000520911 ; ENSP00000430999 ; ENSG00000113269 .
    ENST00000521389 ; ENSP00000430237 ; ENSG00000113269 . [Q86XS8-1 ]
    GeneIDi 55819.
    KEGGi hsa:55819.
    UCSCi uc003mll.1. human.

    Organism-specific databases

    CTDi 55819.
    GeneCardsi GC05M179345.
    HGNCi HGNC:18280. RNF130.
    HPAi HPA014499.
    neXtProti NX_Q86XS8.
    PharmGKBi PA134871556.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG302028.
    HOGENOMi HOG000231432.
    HOVERGENi HBG057659.
    InParanoidi Q86XS8.
    KOi K15701.
    PhylomeDBi Q86XS8.
    TreeFami TF317486.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    ChiTaRSi RNF130. human.
    GenomeRNAii 55819.
    NextBioi 61006.
    PROi Q86XS8.

    Gene expression databases

    ArrayExpressi Q86XS8.
    Bgeei Q86XS8.
    CleanExi HS_RNF130.
    Genevestigatori Q86XS8.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR003137. Protease-assoc_domain.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF02225. PA. 1 hit.
    PF13639. zf-RING_2. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Goliath, a ring-H2 mitochondrial protein, regulated by luteinizing hormone/human chorionic gonadotropin in rat Leydig cells."
      Guais A., Solhonne B., Melaine N., Guellaeen G., Bulle F.
      Biol. Reprod. 70:204-213(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Homo sapiens protein coding cDNA."
      Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    3. "The DNA sequence and comparative analysis of human chromosome 5."
      Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
      , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
      Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Eye, LungImported, Mammary glandImported and SkinImported.
    6. "A novel gene expressed in the human adrenal gland."
      Gu J., Fu S., Ren S., Jin W., Gu Y., Huang Q., Dong H., Yu Y., Fu G., Wang Y., Chen Z., Han Z.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 103-419 (ISOFORM 1).
      Tissue: Adrenal glandImported.
    7. "h-Goliath, paralog of GRAIL, is a new E3 ligase protein, expressed in human leukocytes."
      Guais A., Siegrist S., Solhonne B., Jouault H., Guellaen G., Bulle F.
      Gene 374:112-120(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LACK OF GLYCOSYLATION.
    8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-40.
      Tissue: Liver.
    9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiGOLI_HUMAN
    AccessioniPrimary (citable) accession number: Q86XS8
    Secondary accession number(s): J3KN31
    , Q32P32, Q59EL1, Q641P9, Q6P177, Q7L2U2, Q9P0J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3