Q86XS8 (GOLI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RNF130
EC=6.3.2.-

Alternative name(s):
Goliath homolog
Short name=H-Goliath
RING finger protein 130

Gene names

Name:

RNF130

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a role during the programmed cell death of hematopoietic cells By similarity. Acts as an E3 ubiquitin-protein ligase. Ref.4
Pathway	Protein modification; protein ubiquitination. Ref.4
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Cytoplasm Ref.4.
Tissue specificity	Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts. Ref.4
Post-translational modification	In vivo measurements suggest this protein is glycosylated (Ref.5). In contrast, in vitro experiments failed to detect glycosylation (Ref.4). Ref.4
Sequence similarities	Contains 1 PA (protease associated) domain. Contains 1 RING-type zinc finger.
Sequence caution	The sequence AAF67007.1 differs from that shown. Reason: Frameshift at positions 110 and 116.

Ontologies

Keywords
Biological process	Apoptosis Ubl conjugation pathway
Cellular component	Cytoplasm Membrane
Domain	Signal Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW programmed cell death Inferred from sequence or structural similarity Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA
Molecular_function	ubiquitin-protein ligase activity Inferred from direct assay Ref.4. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 419	392	E3 ubiquitin-protein ligase RNF130		PRO_0000030718
Regions
	Topological domain	28 – 194	167	Extracellular Potential
	Transmembrane	195 – 217	23	Helical; Potential
	Topological domain	218 – 419	202	Cytoplasmic Potential
	Domain	105 – 176	72	PA
	Zinc finger	264 – 305	42	RING-type
Amino acid modifications
	Modified residue	341	1	Phosphoserine Ref.6
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	40	1	N-linked (GlcNAc...) Ref.5
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	114	1	T → A in AAH65244. Ref.2
	Sequence conflict	134	1	N → T in AAF67007. Ref.3
	Sequence conflict	141	1	P → N in AAH82267. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q86XS8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 74E8D987334F3B8E	FASTA	419	46,405
	10 20 30 40 50 60 MSCAGRAGPA RLAALALLTC SLWPARADNA SQEYYTALIN VTVQEPGRGA PLTFRIDRGR 70 80 90 100 110 120 YGLDSPKAEV RGQVLAPLPL HGVADHLGCD PQTRFFVPPN IKQWIALLQR GNCTFKEKIS 130 140 150 160 170 180 RAAFHNAVAV VIYNNKSKEE PVTMTHPGTG DIIAVMITEL RGKDILSYLE KNISVQMTIA 190 200 210 220 230 240 VGTRMPPKNF SRGSLVFVSI SFIVLMIISS AWLIFYFIQK IRYTNARDRN QRRLGDAAKK 250 260 270 280 290 300 AISKLTTRTV KKGDKETDPD FDHCAVCIES YKQNDVVRIL PCKHVFHKSC VDPWLSEHCT 310 320 330 340 350 360 CPMCKLNILK ALGIVPNLPC TDNVAFDMER LTRTQAVNRR SALGDLAGDN SLGLEPLRTS 370 380 390 400 410 GISPLPQDGE LTPRTGEINI AVTKEWFIIA SFGLLSALTL CYMIIRATAS LNANEVEWF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Goliath, a ring-H2 mitochondrial protein, regulated by luteinizing hormone/human chorionic gonadotropin in rat Leydig cells." Guais A., Solhonne B., Melaine N., Guellaeen G., Bulle F. Biol. Reprod. 70:204-213(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye, Lung, Mammary gland and Skin.
[3]	"A novel gene expressed in the human adrenal gland." Gu J., Fu S., Ren S., Jin W., Gu Y., Huang Q., Dong H., Yu Y., Fu G., Wang Y., Chen Z., Han Z. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 103-419. Tissue: Adrenal gland.
[4]	"h-Goliath, paralog of GRAIL, is a new E3 ligase protein, expressed in human leukocytes." Guais A., Siegrist S., Solhonne B., Jouault H., Guellaen G., Bulle F. Gene 374:112-120(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LACK OF GLYCOSYLATION.
[5]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-40, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY083998 mRNA. Translation: AAM08686.1. BC017100 mRNA. Translation: AAH17100.2. BC065244 mRNA. Translation: AAH65244.1. BC082267 mRNA. Translation: AAH82267.1. BC108306 mRNA. Translation: AAI08307.1. AF155650 mRNA. Translation: AAF67007.1. Frameshift.
IPI	IPI00183643.
RefSeq	NP_060904.2. NM_018434.4.
UniGene	Hs.484363.
3D structure databases
ProteinModelPortal	Q86XS8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86XS8. 8 interactions.
MINT	MINT-1388856.
PTM databases
PhosphoSite	Q86XS8.
Polymorphism databases
DMDM	56749089.
Proteomic databases
PaxDb	Q86XS8.
PRIDE	Q86XS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000520911; ENSP00000430999; ENSG00000113269. ENST00000521389; ENSP00000430237; ENSG00000113269.
GeneID	55819.
KEGG	hsa:55819.
UCSC	uc003mll.1. human.
Organism-specific databases
CTD	55819.
GeneCards	GC05M179345.
HGNC	HGNC:18280. RNF130.
HPA	HPA014499.
neXtProt	NX_Q86XS8.
PharmGKB	PA134871556.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG302028.
HOGENOM	HOG000231432.
HOVERGEN	HBG057659.
InParanoid	Q86XS8.
KO	K15701.
OrthoDB	EOG40VVQ5.
PhylomeDB	Q86XS8.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q86XS8.
Bgee	Q86XS8.
CleanEx	HS_RNF130.
Genevestigator	Q86XS8.
GermOnline	ENSG00000113269. Homo sapiens.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR003137. Protease-assoc_domain. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF02225. PA. 1 hit. PF13639. zf-RING_2. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RNF130. human.
GenomeRNAi	55819.
NextBio	61006.

Entry information

Entry name

GOLI_HUMAN

Accession

Primary (citable) accession number: Q86XS8
Secondary accession number(s): Q32P32 , Q641P9, Q6P177, Q7L2U2, Q9P0J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.