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Q86XS8

- GOLI_HUMAN

UniProt

Q86XS8 - GOLI_HUMAN

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Protein

E3 ubiquitin-protein ligase RNF130

Gene

RNF130

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May have a role during the programmed cell death of hematopoietic cells (By similarity). Acts as an E3 ubiquitin-protein ligase.By similarity1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri264 – 30542RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. programmed cell death Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF130 (EC:6.3.2.-)
Alternative name(s):
Goliath homolog
Short name:
H-Goliath
RING finger protein 130
Gene namesi
Name:RNF130Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:18280. RNF130.

Subcellular locationi

Membrane Curated; Single-pass type I membrane protein Curated. Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134871556.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 419392E3 ubiquitin-protein ligase RNF130PRO_0000030718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi40 – 401N-linked (GlcNAc...)1 Publication
Glycosylationi112 – 1121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Modified residuei341 – 3411Phosphoserine1 Publication

Post-translational modificationi

In vivo measurements suggest this protein is glycosylated (PubMed:19159218). In contrast, in vitro experiments failed to detect glycosylation (PubMed:16549277).2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ86XS8.
PaxDbiQ86XS8.
PRIDEiQ86XS8.

PTM databases

PhosphoSiteiQ86XS8.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts.1 Publication

Gene expression databases

BgeeiQ86XS8.
CleanExiHS_RNF130.
ExpressionAtlasiQ86XS8. baseline and differential.
GenevestigatoriQ86XS8.

Organism-specific databases

HPAiHPA014499.

Interactioni

Protein-protein interaction databases

BioGridi120928. 23 interactions.
IntActiQ86XS8. 17 interactions.
MINTiMINT-1388856.

Structurei

3D structure databases

ProteinModelPortaliQ86XS8.
SMRiQ86XS8. Positions 27-187, 264-305.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 194167ExtracellularSequence AnalysisAdd
BLAST
Topological domaini218 – 419202CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei195 – 21723HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini105 – 17672PAAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri264 – 30542RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiNOG302028.
GeneTreeiENSGT00760000119057.
HOGENOMiHOG000231432.
HOVERGENiHBG057659.
InParanoidiQ86XS8.
KOiK15701.
PhylomeDBiQ86XS8.
TreeFamiTF317486.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR003137. Protease-assoc_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q86XS8) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCAGRAGPA RLAALALLTC SLWPARADNA SQEYYTALIN VTVQEPGRGA
60 70 80 90 100
PLTFRIDRGR YGLDSPKAEV RGQVLAPLPL HGVADHLGCD PQTRFFVPPN
110 120 130 140 150
IKQWIALLQR GNCTFKEKIS RAAFHNAVAV VIYNNKSKEE PVTMTHPGTG
160 170 180 190 200
DIIAVMITEL RGKDILSYLE KNISVQMTIA VGTRMPPKNF SRGSLVFVSI
210 220 230 240 250
SFIVLMIISS AWLIFYFIQK IRYTNARDRN QRRLGDAAKK AISKLTTRTV
260 270 280 290 300
KKGDKETDPD FDHCAVCIES YKQNDVVRIL PCKHVFHKSC VDPWLSEHCT
310 320 330 340 350
CPMCKLNILK ALGIVPNLPC TDNVAFDMER LTRTQAVNRR SALGDLAGDN
360 370 380 390 400
SLGLEPLRTS GISPLPQDGE LTPRTGEINI AVTKEWFIIA SFGLLSALTL
410
CYMIIRATAS LNANEVEWF
Length:419
Mass (Da):46,405
Last modified:June 1, 2003 - v1
Checksum:i74E8D987334F3B8E
GO
Isoform 2 (identifier: Q86XS8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-384: K → R
     385-419: Missing.

Note: No experimental confirmation available.

Show »
Length:384
Mass (Da):42,456
Checksum:i76C96ED33B701F28
GO

Sequence cautioni

The sequence AAF67007.1 differs from that shown. Reason: Frameshift at positions 110 and 116.
The sequence BAD93037.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141T → A in AAH65244. (PubMed:15489334)Curated
Sequence conflicti134 – 1341N → T in AAF67007. 1 PublicationCurated
Sequence conflicti141 – 1411P → N in AAH82267. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei384 – 3841K → R in isoform 2. 1 PublicationVSP_055642
Alternative sequencei385 – 41935Missing in isoform 2. 1 PublicationVSP_055643Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY083998 mRNA. Translation: AAM08686.1.
AB209800 mRNA. Translation: BAD93037.1. Different initiation.
AC010285 Genomic DNA. No translation available.
AC026413 Genomic DNA. No translation available.
AC122713 Genomic DNA. No translation available.
CH471165 Genomic DNA. Translation: EAW53773.1.
BC017100 mRNA. Translation: AAH17100.2.
BC065244 mRNA. Translation: AAH65244.1.
BC082267 mRNA. Translation: AAH82267.1.
BC108306 mRNA. Translation: AAI08307.1.
AF155650 mRNA. Translation: AAF67007.1. Frameshift.
CCDSiCCDS4451.1. [Q86XS8-1]
CCDS64340.1. [Q86XS8-2]
RefSeqiNP_001267730.1. NM_001280801.1. [Q86XS8-2]
NP_060904.2. NM_018434.5. [Q86XS8-1]
UniGeneiHs.484363.

Genome annotation databases

EnsembliENST00000261947; ENSP00000261947; ENSG00000113269. [Q86XS8-2]
ENST00000520911; ENSP00000430999; ENSG00000113269.
ENST00000521389; ENSP00000430237; ENSG00000113269. [Q86XS8-1]
GeneIDi55819.
KEGGihsa:55819.
UCSCiuc003mll.1. human. [Q86XS8-1]

Polymorphism databases

DMDMi56749089.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY083998 mRNA. Translation: AAM08686.1 .
AB209800 mRNA. Translation: BAD93037.1 . Different initiation.
AC010285 Genomic DNA. No translation available.
AC026413 Genomic DNA. No translation available.
AC122713 Genomic DNA. No translation available.
CH471165 Genomic DNA. Translation: EAW53773.1 .
BC017100 mRNA. Translation: AAH17100.2 .
BC065244 mRNA. Translation: AAH65244.1 .
BC082267 mRNA. Translation: AAH82267.1 .
BC108306 mRNA. Translation: AAI08307.1 .
AF155650 mRNA. Translation: AAF67007.1 . Frameshift.
CCDSi CCDS4451.1. [Q86XS8-1 ]
CCDS64340.1. [Q86XS8-2 ]
RefSeqi NP_001267730.1. NM_001280801.1. [Q86XS8-2 ]
NP_060904.2. NM_018434.5. [Q86XS8-1 ]
UniGenei Hs.484363.

3D structure databases

ProteinModelPortali Q86XS8.
SMRi Q86XS8. Positions 27-187, 264-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 120928. 23 interactions.
IntActi Q86XS8. 17 interactions.
MINTi MINT-1388856.

PTM databases

PhosphoSitei Q86XS8.

Polymorphism databases

DMDMi 56749089.

Proteomic databases

MaxQBi Q86XS8.
PaxDbi Q86XS8.
PRIDEi Q86XS8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000261947 ; ENSP00000261947 ; ENSG00000113269 . [Q86XS8-2 ]
ENST00000520911 ; ENSP00000430999 ; ENSG00000113269 .
ENST00000521389 ; ENSP00000430237 ; ENSG00000113269 . [Q86XS8-1 ]
GeneIDi 55819.
KEGGi hsa:55819.
UCSCi uc003mll.1. human. [Q86XS8-1 ]

Organism-specific databases

CTDi 55819.
GeneCardsi GC05M179345.
HGNCi HGNC:18280. RNF130.
HPAi HPA014499.
neXtProti NX_Q86XS8.
PharmGKBi PA134871556.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG302028.
GeneTreei ENSGT00760000119057.
HOGENOMi HOG000231432.
HOVERGENi HBG057659.
InParanoidi Q86XS8.
KOi K15701.
PhylomeDBi Q86XS8.
TreeFami TF317486.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

ChiTaRSi RNF130. human.
GenomeRNAii 55819.
NextBioi 35534803.
PROi Q86XS8.

Gene expression databases

Bgeei Q86XS8.
CleanExi HS_RNF130.
ExpressionAtlasi Q86XS8. baseline and differential.
Genevestigatori Q86XS8.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR003137. Protease-assoc_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF02225. PA. 1 hit.
PF13639. zf-RING_2. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Goliath, a ring-H2 mitochondrial protein, regulated by luteinizing hormone/human chorionic gonadotropin in rat Leydig cells."
    Guais A., Solhonne B., Melaine N., Guellaeen G., Bulle F.
    Biol. Reprod. 70:204-213(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Homo sapiens protein coding cDNA."
    Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye, LungImported, Mammary glandImported and SkinImported.
  6. "A novel gene expressed in the human adrenal gland."
    Gu J., Fu S., Ren S., Jin W., Gu Y., Huang Q., Dong H., Yu Y., Fu G., Wang Y., Chen Z., Han Z.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 103-419 (ISOFORM 1).
    Tissue: Adrenal glandImported.
  7. "h-Goliath, paralog of GRAIL, is a new E3 ligase protein, expressed in human leukocytes."
    Guais A., Siegrist S., Solhonne B., Jouault H., Guellaen G., Bulle F.
    Gene 374:112-120(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PATHWAY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, LACK OF GLYCOSYLATION.
  8. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-40.
    Tissue: Liver.
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiGOLI_HUMAN
AccessioniPrimary (citable) accession number: Q86XS8
Secondary accession number(s): J3KN31
, Q32P32, Q59EL1, Q641P9, Q6P177, Q7L2U2, Q9P0J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3