Q86XQ3 (CTSR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cation channel sperm-associated protein 3
Short name=CatSper3

Alternative name(s):
Ca(v)-like protein
One-repeat calcium channel-like protein

Gene names

Name:

CATSPER3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Ref.5 Ref.6
Subunit structure	Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 Potential. Component of the CatSper complex. Interacts with CATSPER1 By similarity.
Subcellular location	Cell projection › cilium › flagellum membrane; Multi-pass membrane protein. Note: Specifically located in the principal piece of sperm tail.
Tissue specificity	Testis-specific. Ref.1 Ref.3 Ref.4
Sequence similarities	Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Differentiation Ion transport Spermatogenesis Transport
Cellular component	Cell membrane Cell projection Cilium Flagellum Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Calcium channel Developmental protein Ion channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW ciliary or flagellar motility Inferred from electronic annotation. Source: Compara multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW sodium ion transport Inferred from electronic annotation. Source: Compara sperm motility Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	CatSper complex Inferred from sequence or structural similarity. Source: UniProtKB acrosomal vesicle Inferred from electronic annotation. Source: Compara cilium Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: Compara
Molecular_function	calcium activated cation channel activity Inferred from electronic annotation. Source: Compara calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW voltage-gated ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Cation channel sperm-associated protein 3		PRO_0000295679
Regions
	Topological domain	1 – 50	50	Cytoplasmic Potential
	Transmembrane	51 – 71	21	Helical; Name=Segment S1; Potential
	Topological domain	72 – 78	7	Extracellular Potential
	Transmembrane	79 – 100	22	Helical; Name=Segment S2; Potential
	Topological domain	101 – 115	15	Cytoplasmic Potential
	Transmembrane	116 – 136	21	Helical; Name=Segment S3; Potential
	Topological domain	137 – 141	5	Extracellular Potential
	Transmembrane	142 – 161	20	Helical; Name=Segment S4; Potential
	Topological domain	162 – 179	18	Cytoplasmic Potential
	Transmembrane	180 – 200	21	Helical; Name=Segment S5; Potential
	Topological domain	201 – 209	9	Extracellular Potential
	Intramembrane	210 – 230	21	Helical; Pore-forming; Potential
	Topological domain	231 – 248	18	Extracellular Potential
	Transmembrane	249 – 269	21	Helical; Name=Segment S6; Potential
Natural variations
	Natural variant	204	1	N → K. Corresponds to variant rs3896260 [ dbSNP | Ensembl ].		VAR_033309

Sequences

	Sequence		Length	Mass (Da)
	Q86XQ3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FB460F66F367DB69	FASTA	398	46,422
	10 20 30 40 50 60 MSQHRHQRHS RVISSSPVDT TSVGFCPTFK KFKRNDDECR AFVKRVIMSR FFKIIMISTV 70 80 90 100 110 120 TSNAFFMALW TSYDIRYRLF RLLEFSEIFF VSICTSELSM KVYVDPINYW KNGYNLLDVI 130 140 150 160 170 180 IIIVMFLPYA LRQLMGKQFT YLYIADGMQS LRILKLIGYS QGIRTLITAV GQTVYTVASV 190 200 210 220 230 240 LLLLFLLMYI FAILGFCLFG SPDNGDHDNW GNLAAAFFTL FSLATVDGWT DLQKQLDNRE 250 260 270 280 290 300 FALSRAFTII FILLASFIFL NMFVGVMIMH TEDSIRKFER ELMLEQQEML MGEKQVILQR 310 320 330 340 350 360 QQEEISRLMH IQKNADCTSF SELVENFKKT LSHTDPMVLD DFGTSLPFID IYFSTLDYQD 370 380 390 TTVHKLQELY YEIVHVLSLM LEDLPQEKPQ SLEKVDEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a novel one-repeat calcium channel-like gene." Arias J.M., Murbartian J., Perez-Reyes E. Biochem. Biophys. Res. Commun. 303:31-36(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Cerebellum.
[3]	"Identification of human and mouse CatSper3 and CatSper4 genes: characterisation of a common interaction domain and evidence for expression in testis." Lobley A., Pierron V., Reynolds L., Allen L., Michalovich D. Reprod. Biol. Endocrinol. 1:53-53(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[4]	"Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility." Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L. Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm." Strunker T., Goodwin N., Brenker C., Kashikar N.D., Weyand I., Seifert R., Kaupp U.B. Nature 471:382-386(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
[6]	"Progesterone activates the principal Ca2+ channel of human sperm." Lishko P.V., Botchkina I.L., Kirichok Y. Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF432876 mRNA. Translation: AAO85416.1. AY156951 mRNA. Translation: AAO13012.1. BC101692 mRNA. Translation: AAI01693.1. BC110384 mRNA. Translation: AAI10385.1. BN000272 mRNA. Translation: CAE30476.1.
IPI	IPI00256795.
RefSeq	NP_821138.1. NM_178019.2.
UniGene	Hs.631804.
3D structure databases
ProteinModelPortal	Q86XQ3.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000282611.
PTM databases
PhosphoSite	Q86XQ3.
Polymorphism databases
DMDM	74714131.
Proteomic databases
PaxDb	Q86XQ3.
PRIDE	Q86XQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000282611; ENSP00000282611; ENSG00000152705.
GeneID	347732.
KEGG	hsa:347732.
UCSC	uc003lag.3. human.
Organism-specific databases
CTD	347732.
GeneCards	GC05P134331.
HGNC	HGNC:20819. CATSPER3.
MIM	609120. gene.
neXtProt	NX_Q86XQ3.
PharmGKB	PA134911185.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG117719.
HOGENOM	HOG000112052.
HOVERGEN	HBG099875.
InParanoid	Q86XQ3.
OMA	YWKDGYN.
OrthoDB	EOG4SN1NX.
PhylomeDB	Q86XQ3.
Gene expression databases
Bgee	Q86XQ3.
CleanEx	HS_CATSPER3.
Genevestigator	Q86XQ3.
Family and domain databases
InterPro	IPR005821. Ion_trans_dom. [Graphical view]
Pfam	PF00520. Ion_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	347732.
NextBio	99258.
SOURCE	Search...

Entry information

Entry name

CTSR3_HUMAN

Accession

Primary (citable) accession number: Q86XQ3
Secondary accession number(s): Q86XS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.