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Q86XQ3 (CTSR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cation channel sperm-associated protein 3

Short name=CatSper3
Alternative name(s):
Ca(v)-like protein
One-repeat calcium channel-like protein
Gene names
Name:CATSPER3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Ref.5 Ref.6

Subunit structure

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 Potential. Component of the CatSper complex. Interacts with CATSPER1 By similarity.

Subcellular location

Cell projectionciliumflagellum membrane; Multi-pass membrane protein. Note: Specifically located in the principal piece of sperm tail.

Tissue specificity

Testis-specific. Ref.1 Ref.3 Ref.4

Sequence similarities

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family. [View classification]

Ontologies

Keywords
   Biological processCalcium transport
Differentiation
Ion transport
Spermatogenesis
Transport
   Cellular componentCell membrane
Cell projection
Cilium
Flagellum
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Developmental protein
Ion channel
Voltage-gated channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcalcium ion import

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

cilium or flagellum-dependent cell motility

Inferred from electronic annotation. Source: Ensembl

membrane depolarization during action potential

Inferred from Biological aspect of Ancestor. Source: RefGenome

multicellular organism reproduction

Traceable author statement. Source: Reactome

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

single fertilization

Traceable author statement. Source: Reactome

sodium ion transport

Inferred from electronic annotation. Source: Ensembl

sperm motility

Inferred from Biological aspect of Ancestor. Source: RefGenome

sperm-egg recognition

Traceable author statement. Source: Reactome

spermatogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCatSper complex

Inferred from sequence or structural similarity. Source: UniProtKB

acrosomal vesicle

Inferred from electronic annotation. Source: Ensembl

cilium

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functioncalcium activated cation channel activity

Inferred from electronic annotation. Source: Ensembl

voltage-gated calcium channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Cation channel sperm-associated protein 3
PRO_0000295679

Regions

Topological domain1 – 5050Cytoplasmic Potential
Transmembrane51 – 7121Helical; Name=Segment S1; Potential
Topological domain72 – 787Extracellular Potential
Transmembrane79 – 10022Helical; Name=Segment S2; Potential
Topological domain101 – 11515Cytoplasmic Potential
Transmembrane116 – 13621Helical; Name=Segment S3; Potential
Topological domain137 – 1415Extracellular Potential
Transmembrane142 – 16120Helical; Name=Segment S4; Potential
Topological domain162 – 17918Cytoplasmic Potential
Transmembrane180 – 20021Helical; Name=Segment S5; Potential
Topological domain201 – 2099Extracellular Potential
Intramembrane210 – 23021Helical; Pore-forming; Potential
Topological domain231 – 24818Extracellular Potential
Transmembrane249 – 26921Helical; Name=Segment S6; Potential

Natural variations

Natural variant2041N → K.
Corresponds to variant rs3896260 [ dbSNP | Ensembl ].
VAR_033309

Sequences

Sequence LengthMass (Da)Tools
Q86XQ3 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: FB460F66F367DB69

FASTA39846,422
        10         20         30         40         50         60 
MSQHRHQRHS RVISSSPVDT TSVGFCPTFK KFKRNDDECR AFVKRVIMSR FFKIIMISTV 

        70         80         90        100        110        120 
TSNAFFMALW TSYDIRYRLF RLLEFSEIFF VSICTSELSM KVYVDPINYW KNGYNLLDVI 

       130        140        150        160        170        180 
IIIVMFLPYA LRQLMGKQFT YLYIADGMQS LRILKLIGYS QGIRTLITAV GQTVYTVASV 

       190        200        210        220        230        240 
LLLLFLLMYI FAILGFCLFG SPDNGDHDNW GNLAAAFFTL FSLATVDGWT DLQKQLDNRE 

       250        260        270        280        290        300 
FALSRAFTII FILLASFIFL NMFVGVMIMH TEDSIRKFER ELMLEQQEML MGEKQVILQR 

       310        320        330        340        350        360 
QQEEISRLMH IQKNADCTSF SELVENFKKT LSHTDPMVLD DFGTSLPFID IYFSTLDYQD 

       370        380        390 
TTVHKLQELY YEIVHVLSLM LEDLPQEKPQ SLEKVDEK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a novel one-repeat calcium channel-like gene."
Arias J.M., Murbartian J., Perez-Reyes E.
Biochem. Biophys. Res. Commun. 303:31-36(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain and Cerebellum.
[3]"Identification of human and mouse CatSper3 and CatSper4 genes: characterisation of a common interaction domain and evidence for expression in testis."
Lobley A., Pierron V., Reynolds L., Allen L., Michalovich D.
Reprod. Biol. Endocrinol. 1:53-53(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
[4]"Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility."
Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L.
Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The CatSper channel mediates progesterone-induced Ca2+ influx in human sperm."
Strunker T., Goodwin N., Brenker C., Kashikar N.D., Weyand I., Seifert R., Kaupp U.B.
Nature 471:382-386(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
[6]"Progesterone activates the principal Ca2+ channel of human sperm."
Lishko P.V., Botchkina I.L., Kirichok Y.
Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN ACTIVATION BY PROGESTERONE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF432876 mRNA. Translation: AAO85416.1.
AY156951 mRNA. Translation: AAO13012.1.
BC101692 mRNA. Translation: AAI01693.1.
BC110384 mRNA. Translation: AAI10385.1.
BN000272 mRNA. Translation: CAE30476.1.
CCDSCCDS4181.1.
RefSeqNP_821138.1. NM_178019.2.
UniGeneHs.631804.

3D structure databases

ProteinModelPortalQ86XQ3.
SMRQ86XQ3. Positions 43-315.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000282611.

Chemistry

GuidetoPHARMACOLOGY390.

Protein family/group databases

TCDB1.A.1.19.1. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteQ86XQ3.

Polymorphism databases

DMDM74714131.

Proteomic databases

PaxDbQ86XQ3.
PRIDEQ86XQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000282611; ENSP00000282611; ENSG00000152705.
GeneID347732.
KEGGhsa:347732.
UCSCuc003lag.3. human.

Organism-specific databases

CTD347732.
GeneCardsGC05P134331.
HGNCHGNC:20819. CATSPER3.
MIM609120. gene.
neXtProtNX_Q86XQ3.
PharmGKBPA134911185.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG117719.
HOGENOMHOG000112052.
HOVERGENHBG099875.
InParanoidQ86XQ3.
KOK16891.
OMAYWKDGYN.
OrthoDBEOG7PVWQD.
PhylomeDBQ86XQ3.
TreeFamTF343841.

Enzyme and pathway databases

ReactomeREACT_163848. Reproduction.

Gene expression databases

BgeeQ86XQ3.
CleanExHS_CATSPER3.
GenevestigatorQ86XQ3.

Family and domain databases

InterProIPR028739. Cat_Sper_3.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERPTHR10037:SF197. PTHR10037:SF197. 1 hit.
PfamPF00520. Ion_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCatSper3.
GenomeRNAi347732.
NextBio99258.
PROQ86XQ3.
SOURCESearch...

Entry information

Entry nameCTSR3_HUMAN
AccessionPrimary (citable) accession number: Q86XQ3
Secondary accession number(s): Q86XS6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM