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Q86XP1 (DGKH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase eta

Short name=DAG kinase eta
EC=2.7.1.107
Alternative name(s):
Diglyceride kinase eta
Short name=DGK-eta
Gene names
Name:DGKH
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization. Ref.1 Ref.6

Catalytic activity

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1

Subunit structure

Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF. Ref.1 Ref.6

Subcellular location

Cytoplasm. Cell membrane. Note: Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS. Ref.1 Ref.6

Tissue specificity

Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon. Ref.1

Post-translational modification

Phosphorylated; does not inhibit catalytic activity. Ref.1

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 1 PH domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 SAM (sterile alpha motif) domain.

Sequence caution

The sequence BAC05280.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86XP1-1)

Also known as: DAG kinase eta-2; DGKH-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86XP1-2)

Also known as: DAG kinase eta-1; DGKH-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1148-1220: VQKWGTEEVA...LGRSTPQSEV → GSGDTESGSCEANSPGN
Isoform 3 (identifier: Q86XP1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.
     1191-1191: K → KNTVGEKRDTKENGKHM
Note: No experimental confirmation available.
Isoform 4 (identifier: Q86XP1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12201220Diacylglycerol kinase eta
PRO_0000239367

Regions

Domain65 – 15894PH
Domain328 – 463136DAGKc
Domain1151 – 121464SAM
Zinc finger175 – 22551Phorbol-ester/DAG-type 1
Zinc finger247 – 29852Phorbol-ester/DAG-type 2
Motif1218 – 12203PDZ-binding

Natural variations

Alternative sequence1 – 136136Missing in isoform 3 and isoform 4.
VSP_019183
Alternative sequence1148 – 122073VQKWG…PQSEV → GSGDTESGSCEANSPGN in isoform 2.
VSP_019185
Alternative sequence11911K → KNTVGEKRDTKENGKHM in isoform 3.
VSP_019186
Natural variant12011V → A.
Corresponds to variant rs17646069 [ dbSNP | Ensembl ].
VAR_033867

Experimental info

Mutagenesis11511W → G: Abolishes homo and heterooligomerization but not its catalytic activity. Ref.1
Sequence conflict6771I → M in BAC05280. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (DAG kinase eta-2) (DGKH-2) [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: F1BBF5B7FA582C97

FASTA1,220134,866
        10         20         30         40         50         60 
MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK LIRKVSTSGQ 

        70         80         90        100        110        120 
IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS KSLIFDEVDL SDASVAEAST 

       130        140        150        160        170        180 
KNANNSFTII TPFRRLMLCA ENRKEMEDWI SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA 

       190        200        210        220        230        240 
CSHARPTFCN VCRESLSGVT SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED 

       250        260        270        280        290        300 
EDGVAMPHQW LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL 

       310        320        330        340        350        360 
GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL RRFKQLLNPA 

       370        380        390        400        410        420 
QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE IDKLNLNKQC QLGVLPLGTG 

       430        440        450        460        470        480 
NDLARVLGWG GSYDDDTQLP QILEKLERAS TKMLDRWSIM TYELKLPPKA SLLPGPPEAS 

       490        500        510        520        530        540 
EEFYMTIYED SVATHLTKIL NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA 

       550        560        570        580        590        600 
DAVASKCSVL NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG 

       610        620        630        640        650        660 
DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA NQSSDYDSTE 

       670        680        690        700        710        720 
TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV PGPAVAASKE NLPVLNTRII 

       730        740        750        760        770        780 
CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD 

       790        800        810        820        830        840 
AKISLEFNNK REEHPEKCRS RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP 

       850        860        870        880        890        900 
SLQGIAVLNI PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH 

       910        920        930        940        950        960 
HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE 

       970        980        990       1000       1010       1020 
DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE ELITRICDAA TIHCLLEQEL 

      1030       1040       1050       1060       1070       1080 
AHAVNACSHA LNKANPRCPE SLTRDTATEI AINVKALYNE TESLLVGRVP LQLESPHEER 

      1090       1100       1110       1120       1130       1140 
VSNALHSVEV ELQKLTEIPW LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK 

      1150       1160       1170       1180       1190       1200 
QKTSSQPVQK WGTEEVAAWL DLLNLGEYKD IFIRHDIRGA ELLHLERRDL KDLGIPKVGH 

      1210       1220 
VKRILQGIKE LGRSTPQSEV 

« Hide

Isoform 2 (DAG kinase eta-1) (DGKH-1) [UniParc].

Checksum: DF39B04BECC0B629
Show »

FASTA1,164128,035
Isoform 3 [UniParc].

Checksum: 0D36C1FA1E7C1A43
Show »

FASTA1,100122,392
Isoform 4 [UniParc].

Checksum: 8994FBC10F6DB99D
Show »

FASTA1,084120,566

References

« Hide 'large scale' references
[1]"Identification and characterization of two splice variants of human diacylglycerol kinase eta."
Murakami T., Sakane F., Imai S., Houkin K., Kanoh H.
J. Biol. Chem. 278:34364-34372(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, MUTAGENESIS OF TRP-1151.
Tissue: Testis.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 640-1220 (ISOFORM 2).
Tissue: Testis and Uterus.
[3]"The DNA sequence and analysis of human chromosome 13."
Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T.
Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Testis.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 617-1220 (ISOFORM 1).
Tissue: Amygdala.
[6]"Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf heterodimerization."
Yasuda S., Kai M., Imai S., Takeishi K., Taketomi A., Toyota M., Kanoh H., Sakane F.
J. Biol. Chem. 284:29559-29570(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAF1 AND BRAF.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB078967 mRNA. Translation: BAC66960.1.
AB078968 mRNA. Translation: BAC66961.1.
AK302727 mRNA. Translation: BAG63946.1.
AL136527, AL139328, AL157932 Genomic DNA. Translation: CAH71399.2.
AL136527, AL139328, AL157932 Genomic DNA. Translation: CAM14245.1.
AL139328, AL136527, AL157932 Genomic DNA. Translation: CAI16894.2.
AL139328, AL136527, AL157932 Genomic DNA. Translation: CAM23676.1.
AL157932, AL136527, AL139328 Genomic DNA. Translation: CAH71062.2.
AL157932, AL136527, AL139328 Genomic DNA. Translation: CAM13770.1.
BC043292 mRNA. Translation: AAH43292.1.
AL833883 mRNA. Translation: CAD38739.1.
AK098302 mRNA. Translation: BAC05280.1. Different initiation.
CCDSCCDS55898.1. [Q86XP1-3]
CCDS55899.1. [Q86XP1-5]
CCDS9381.1. [Q86XP1-1]
CCDS9382.1. [Q86XP1-2]
RefSeqNP_001191433.1. NM_001204504.1. [Q86XP1-2]
NP_001191434.1. NM_001204505.1. [Q86XP1-3]
NP_001191435.1. NM_001204506.1. [Q86XP1-5]
NP_690874.2. NM_152910.4. [Q86XP1-2]
NP_821077.1. NM_178009.3. [Q86XP1-1]
UniGeneHs.733239.

3D structure databases

ProteinModelPortalQ86XP1.
SMRQ86XP1. Positions 68-154, 228-292, 1147-1214.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127768. 5 interactions.
IntActQ86XP1. 2 interactions.
STRING9606.ENSP00000337572.

PTM databases

PhosphoSiteQ86XP1.

Polymorphism databases

DMDM74762463.

Proteomic databases

MaxQBQ86XP1.
PaxDbQ86XP1.
PRIDEQ86XP1.

Protocols and materials databases

DNASU160851.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261491; ENSP00000261491; ENSG00000102780. [Q86XP1-2]
ENST00000337343; ENSP00000337572; ENSG00000102780. [Q86XP1-1]
ENST00000379274; ENSP00000368576; ENSG00000102780. [Q86XP1-3]
ENST00000536612; ENSP00000445114; ENSG00000102780. [Q86XP1-5]
ENST00000540693; ENSP00000440823; ENSG00000102780. [Q86XP1-2]
GeneID160851.
KEGGhsa:160851.
UCSCuc001uyl.2. human. [Q86XP1-1]
uc001uym.2. human. [Q86XP1-2]
uc001uyo.2. human. [Q86XP1-3]

Organism-specific databases

CTD160851.
GeneCardsGC13P042622.
HGNCHGNC:2854. DGKH.
HPAHPA039533.
HPA040355.
MIM604071. gene.
neXtProtNX_Q86XP1.
PharmGKBPA27315.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG328046.
HOVERGENHBG051346.
InParanoidQ86XP1.
KOK00901.
OMAYEVAQFN.
OrthoDBEOG7DVD9B.
PhylomeDBQ86XP1.
TreeFamTF313104.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_604. Hemostasis.

Gene expression databases

ArrayExpressQ86XP1.
BgeeQ86XP1.
CleanExHS_DGKH.
GenevestigatorQ86XP1.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
2.30.29.30. 1 hit.
InterProIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
[Graphical view]
PfamPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF00169. PH. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00233. PH. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 2 hits.
SSF47769. SSF47769. 1 hit.
PROSITEPS50146. DAGK. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDGKH. human.
GenomeRNAi160851.
NextBio88001.
PROQ86XP1.
SOURCESearch...

Entry information

Entry nameDGKH_HUMAN
AccessionPrimary (citable) accession number: Q86XP1
Secondary accession number(s): A2A2W7 expand/collapse secondary AC list , A6NFX7, B4DZ34, Q5VZW0, Q6PI56, Q86XP2, Q8N3N0, Q8N7J9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 13

Human chromosome 13: entries, gene names and cross-references to MIM