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Protein

Diacylglycerol kinase eta

Gene

DGKH

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.2 Publications

Caution

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 225Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • diacylglycerol kinase activity Source: BHF-UCL
  • metal ion binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • phosphatidic acid metabolic process Source: BHF-UCL
  • platelet activation Source: Reactome
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  • protein oligomerization Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 2681
ReactomeiR-HSA-114508 Effects of PIP2 hydrolysis

Chemistry databases

SwissLipidsiSLP:000000928 [Q86XP1-2]

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase eta (EC:2.7.1.107)
Short name:
DAG kinase eta
Alternative name(s):
Diglyceride kinase eta
Short name:
DGK-eta
Gene namesi
Name:DGKH
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000102780.16
HGNCiHGNC:2854 DGKH
MIMi604071 gene
neXtProtiNX_Q86XP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1151W → G: Abolishes homo and heterooligomerization but not its catalytic activity. 1 Publication1

Organism-specific databases

DisGeNETi160851
OpenTargetsiENSG00000102780
PharmGKBiPA27315

Polymorphism and mutation databases

BioMutaiDGKH
DMDMi74762463

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393671 – 1220Diacylglycerol kinase etaAdd BLAST1220

Post-translational modificationi

Phosphorylated; does not inhibit catalytic activity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86XP1
MaxQBiQ86XP1
PaxDbiQ86XP1
PeptideAtlasiQ86XP1
PRIDEiQ86XP1

PTM databases

iPTMnetiQ86XP1
PhosphoSitePlusiQ86XP1

Expressioni

Tissue specificityi

Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon.1 Publication

Gene expression databases

BgeeiENSG00000102780
CleanExiHS_DGKH
ExpressionAtlasiQ86XP1 baseline and differential
GenevisibleiQ86XP1 HS

Organism-specific databases

HPAiHPA039533
HPA040355

Interactioni

Subunit structurei

Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF.2 Publications

Protein-protein interaction databases

BioGridi12776811 interactors.
IntActiQ86XP1 4 interactors.
STRINGi9606.ENSP00000337572

Structurei

3D structure databases

ProteinModelPortaliQ86XP1
SMRiQ86XP1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 158PHPROSITE-ProRule annotationAdd BLAST94
Domaini328 – 463DAGKcPROSITE-ProRule annotationAdd BLAST136
Domaini1151 – 1214SAMPROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1218 – 1220PDZ-binding3

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri175 – 225Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri247 – 298Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST52

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1170 Eukaryota
ENOG410XSJR LUCA
GeneTreeiENSGT00760000119050
HOVERGENiHBG051346
InParanoidiQ86XP1
KOiK00901
OMAiYSEKCVM
OrthoDBiEOG091G00SK
PhylomeDBiQ86XP1
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.301 hit
3.40.50.103301 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR037607 DGK
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001660 SAM
IPR013761 SAM/pointed_sf
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF00169 PH, 1 hit
PF07647 SAM_2, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00233 PH, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF111331 SSF111331, 2 hits
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50105 SAM_DOMAIN, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86XP1-1) [UniParc]FASTAAdd to basket
Also known as: DAG kinase eta-2, DGKH-2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK
60 70 80 90 100
LIRKVSTSGQ IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS
110 120 130 140 150
KSLIFDEVDL SDASVAEAST KNANNSFTII TPFRRLMLCA ENRKEMEDWI
160 170 180 190 200
SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA CSHARPTFCN VCRESLSGVT
210 220 230 240 250
SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED EDGVAMPHQW
260 270 280 290 300
LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL
310 320 330 340 350
GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL
360 370 380 390 400
RRFKQLLNPA QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE
410 420 430 440 450
IDKLNLNKQC QLGVLPLGTG NDLARVLGWG GSYDDDTQLP QILEKLERAS
460 470 480 490 500
TKMLDRWSIM TYELKLPPKA SLLPGPPEAS EEFYMTIYED SVATHLTKIL
510 520 530 540 550
NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA DAVASKCSVL
560 570 580 590 600
NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG
610 620 630 640 650
DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA
660 670 680 690 700
NQSSDYDSTE TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV
710 720 730 740 750
PGPAVAASKE NLPVLNTRII CPGLRAGLAA SIAGSSIINK MLLANIDPFG
760 770 780 790 800
ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD AKISLEFNNK REEHPEKCRS
810 820 830 840 850
RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP SLQGIAVLNI
860 870 880 890 900
PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH
910 920 930 940 950
HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR
960 970 980 990 1000
AFESTLKSWE DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE
1010 1020 1030 1040 1050
ELITRICDAA TIHCLLEQEL AHAVNACSHA LNKANPRCPE SLTRDTATEI
1060 1070 1080 1090 1100
AINVKALYNE TESLLVGRVP LQLESPHEER VSNALHSVEV ELQKLTEIPW
1110 1120 1130 1140 1150
LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK QKTSSQPVQK
1160 1170 1180 1190 1200
WGTEEVAAWL DLLNLGEYKD IFIRHDIRGA ELLHLERRDL KDLGIPKVGH
1210 1220
VKRILQGIKE LGRSTPQSEV
Length:1,220
Mass (Da):134,866
Last modified:June 1, 2003 - v1
Checksum:iF1BBF5B7FA582C97
GO
Isoform 2 (identifier: Q86XP1-2) [UniParc]FASTAAdd to basket
Also known as: DAG kinase eta-1, DGKH-1

The sequence of this isoform differs from the canonical sequence as follows:
     1148-1220: VQKWGTEEVA...LGRSTPQSEV → GSGDTESGSCEANSPGN

Show »
Length:1,164
Mass (Da):128,035
Checksum:iDF39B04BECC0B629
GO
Isoform 3 (identifier: Q86XP1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.
     1191-1191: K → KNTVGEKRDTKENGKHM

Note: No experimental confirmation available.
Show »
Length:1,100
Mass (Da):122,392
Checksum:i0D36C1FA1E7C1A43
GO
Isoform 4 (identifier: Q86XP1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Show »
Length:1,084
Mass (Da):120,566
Checksum:i8994FBC10F6DB99D
GO

Sequence cautioni

The sequence BAC05280 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti677I → M in BAC05280 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0338671201V → A. Corresponds to variant dbSNP:rs17646069Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0191831 – 136Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST136
Alternative sequenceiVSP_0191851148 – 1220VQKWG…PQSEV → GSGDTESGSCEANSPGN in isoform 2. 2 PublicationsAdd BLAST73
Alternative sequenceiVSP_0191861191K → KNTVGEKRDTKENGKHM in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB078967 mRNA Translation: BAC66960.1
AB078968 mRNA Translation: BAC66961.1
AK302727 mRNA Translation: BAG63946.1
AL136527 Genomic DNA No translation available.
AL139328 Genomic DNA No translation available.
AL157932 Genomic DNA No translation available.
BC043292 mRNA Translation: AAH43292.1
AL833883 mRNA Translation: CAD38739.1
AK098302 mRNA Translation: BAC05280.1 Different initiation.
CCDSiCCDS55898.1 [Q86XP1-3]
CCDS55899.1 [Q86XP1-5]
CCDS9381.1 [Q86XP1-1]
CCDS9382.1 [Q86XP1-2]
RefSeqiNP_001191433.1, NM_001204504.2 [Q86XP1-2]
NP_001191434.1, NM_001204505.2 [Q86XP1-3]
NP_001191435.1, NM_001204506.2 [Q86XP1-5]
NP_001284358.1, NM_001297429.1
NP_690874.2, NM_152910.5 [Q86XP1-2]
NP_821077.1, NM_178009.4 [Q86XP1-1]
UniGeneiHs.630380
Hs.659437
Hs.733239

Genome annotation databases

EnsembliENST00000261491; ENSP00000261491; ENSG00000102780 [Q86XP1-2]
ENST00000337343; ENSP00000337572; ENSG00000102780 [Q86XP1-1]
ENST00000379274; ENSP00000368576; ENSG00000102780 [Q86XP1-2]
ENST00000536612; ENSP00000445114; ENSG00000102780 [Q86XP1-3]
ENST00000628433; ENSP00000485809; ENSG00000102780 [Q86XP1-5]
GeneIDi160851
KEGGihsa:160851
UCSCiuc001uyl.3 human [Q86XP1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDGKH_HUMAN
AccessioniPrimary (citable) accession number: Q86XP1
Secondary accession number(s): A2A2W7
, A6NFX7, B4DZ34, Q5VZW0, Q6PI56, Q86XP2, Q8N3N0, Q8N7J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome