Q86XP1 (DGKH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase eta
Short name=DGK-eta

Gene names

Name:

DGKH

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization. Ref.1 Ref.6
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1
Subunit structure	Isoform 1 forms homooligomers through the SAM domain. Isoform 1 is also able to form heterooligomers with SAM domain-containing isoforms of DGKD. Oligomerization of isoform 1 inhibits its catalytic activity. Interacts with RAF1 and BRAF. Ref.1 Ref.6
Subcellular location	Cytoplasm. Cell membrane. Note: Translocated from the cytoplasm to endosomes in response to stress stimuli. Isoform 2 is rapidly relocated back to the cytoplasm upon removal of stress stimuli, whereas isoform 1 exhibits sustained endosomal association. Translocates from the cytoplasm to the cell membrane in the presence of active GTP-bound form of HRAS. Ref.1 Ref.6
Tissue specificity	Isoform 2 is ubiquitously expressed. Isoform 1 is expressed only in testis, kidney and colon. Ref.1
Post-translational modification	Phosphorylated; does not inhibit catalytic activity. Ref.1
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence BAC05280.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro platelet activation Traceable author statement. Source: Reactome protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from mutant phenotype Ref.1. Source: UniProtKB protein oligomerization Inferred from mutant phenotype Ref.1. Source: UniProtKB
Cellular_component	endosome Inferred from direct assay Ref.1. Source: UniProtKB nuclear membrane Inferred from direct assay. Source: HPA plasma membrane Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86XP1-1)

Also known as: DAG kinase eta-2; DGKH-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86XP1-2)

Also known as: DAG kinase eta-1; DGKH-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1148-1220: VQKWGTEEVA...LGRSTPQSEV → GSGDTESGSCEANSPGN

Isoform 3 (identifier: Q86XP1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.
     1191-1191: K → KNTVGEKRDTKENGKHM

Note: No experimental confirmation available.

Isoform 4 (identifier: Q86XP1-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-136: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1220	1220	Diacylglycerol kinase eta		PRO_0000239367
Regions
	Domain	65 – 158	94	PH
	Domain	328 – 463	136	DAGKc
	Domain	1151 – 1214	64	SAM
	Zinc finger	175 – 225	51	Phorbol-ester/DAG-type 1
	Zinc finger	247 – 298	52	Phorbol-ester/DAG-type 2
	Motif	1218 – 1220	3	PDZ-binding
Natural variations
	Alternative sequence	1 – 136	136	Missing in isoform 3 and isoform 4.		VSP_019183
	Alternative sequence	1148 – 1220	73	VQKWG…PQSEV → GSGDTESGSCEANSPGN in isoform 2.		VSP_019185
	Alternative sequence	1191	1	K → KNTVGEKRDTKENGKHM in isoform 3.		VSP_019186
	Natural variant	1201	1	V → A. Corresponds to variant rs17646069 [ dbSNP | Ensembl ].		VAR_033867
Experimental info
	Mutagenesis	1151	1	W → G: Abolishes homo and heterooligomerization but not its catalytic activity. Ref.1
	Sequence conflict	677	1	I → M in BAC05280. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (DAG kinase eta-2) (DGKH-2) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: F1BBF5B7FA582C97	FASTA	1,220	134,866
	10 20 30 40 50 60 MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK LIRKVSTSGQ 70 80 90 100 110 120 IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS KSLIFDEVDL SDASVAEAST 130 140 150 160 170 180 KNANNSFTII TPFRRLMLCA ENRKEMEDWI SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA 190 200 210 220 230 240 CSHARPTFCN VCRESLSGVT SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED 250 260 270 280 290 300 EDGVAMPHQW LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL 310 320 330 340 350 360 GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL RRFKQLLNPA 370 380 390 400 410 420 QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE IDKLNLNKQC QLGVLPLGTG 430 440 450 460 470 480 NDLARVLGWG GSYDDDTQLP QILEKLERAS TKMLDRWSIM TYELKLPPKA SLLPGPPEAS 490 500 510 520 530 540 EEFYMTIYED SVATHLTKIL NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA 550 560 570 580 590 600 DAVASKCSVL NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG 610 620 630 640 650 660 DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA NQSSDYDSTE 670 680 690 700 710 720 TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV PGPAVAASKE NLPVLNTRII 730 740 750 760 770 780 CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD 790 800 810 820 830 840 AKISLEFNNK REEHPEKCRS RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP 850 860 870 880 890 900 SLQGIAVLNI PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH 910 920 930 940 950 960 HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE 970 980 990 1000 1010 1020 DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE ELITRICDAA TIHCLLEQEL 1030 1040 1050 1060 1070 1080 AHAVNACSHA LNKANPRCPE SLTRDTATEI AINVKALYNE TESLLVGRVP LQLESPHEER 1090 1100 1110 1120 1130 1140 VSNALHSVEV ELQKLTEIPW LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK 1150 1160 1170 1180 1190 1200 QKTSSQPVQK WGTEEVAAWL DLLNLGEYKD IFIRHDIRGA ELLHLERRDL KDLGIPKVGH 1210 1220 VKRILQGIKE LGRSTPQSEV
	Isoform 2 (DAG kinase eta-1) (DGKH-1) [UniParc]. Checksum: DF39B04BECC0B629	FASTA	1,164	128,035
	10 20 30 40 50 60 MAGAGGQHHP PGAAGGAAAG AGAAVTSAAA SAGPGEDSSD SEAEQEGPQK LIRKVSTSGQ 70 80 90 100 110 120 IRTKTSIKEG QLLKQTSSFQ RWKKRYFKLR GRTLYYAKDS KSLIFDEVDL SDASVAEAST 130 140 150 160 170 180 KNANNSFTII TPFRRLMLCA ENRKEMEDWI SSLKSVQTRE PYEVAQFNVE HFSGMHNWYA 190 200 210 220 230 240 CSHARPTFCN VCRESLSGVT SHGLSCEVCK FKAHKRCAVR ATNNCKWTTL ASIGKDIIED 250 260 270 280 290 300 EDGVAMPHQW LEGNLPVSAK CAVCDKTCGS VLRLQDWKCL WCKTMVHTAC KDLYHPICPL 310 320 330 340 350 360 GQCKVSIIPP IALNSTDSDG FCRATFSFCV SPLLVFVNSK SGDNQGVKFL RRFKQLLNPA 370 380 390 400 410 420 QVFDLMNGGP HLGLRLFQKF DNFRILVCGG DGSVGWVLSE IDKLNLNKQC QLGVLPLGTG 430 440 450 460 470 480 NDLARVLGWG GSYDDDTQLP QILEKLERAS TKMLDRWSIM TYELKLPPKA SLLPGPPEAS 490 500 510 520 530 540 EEFYMTIYED SVATHLTKIL NSDEHAVVIS SAKTLCETVK DFVAKVEKTY DKTLENAVVA 550 560 570 580 590 600 DAVASKCSVL NEKLEQLLQA LHTDSQAAPV LPGLSPLIVE EDAVESSSEE SLGESKEQLG 610 620 630 640 650 660 DDVTKPSSQK AVKPREIMLR ANSLKKAVRQ VIEEAGKVMD DPTVHPCEPA NQSSDYDSTE 670 680 690 700 710 720 TDESKEEAKD DGAKESITVK TAPRSPDARA SYGHSQTDSV PGPAVAASKE NLPVLNTRII 730 740 750 760 770 780 CPGLRAGLAA SIAGSSIINK MLLANIDPFG ATPFIDPDLD SVDGYSEKCV MNNYFGIGLD 790 800 810 820 830 840 AKISLEFNNK REEHPEKCRS RTKNLMWYGV LGTRELLQRS YKNLEQRVQL ECDGQYIPLP 850 860 870 880 890 900 SLQGIAVLNI PSYAGGTNFW GGTKEDDIFA APSFDDKILE VVAIFDSMQM AVSRVIKLQH 910 920 930 940 950 960 HRIAQCRTVK ITIFGDEGVP VQVDGEAWVQ PPGIIKIVHK NRAQMLTRDR AFESTLKSWE 970 980 990 1000 1010 1020 DKQKCDSGKP VLRTHLYIHH AIDLATEEVS QMQLCSQAAE ELITRICDAA TIHCLLEQEL 1030 1040 1050 1060 1070 1080 AHAVNACSHA LNKANPRCPE SLTRDTATEI AINVKALYNE TESLLVGRVP LQLESPHEER 1090 1100 1110 1120 1130 1140 VSNALHSVEV ELQKLTEIPW LYYILHPNED EEPPMDCTKR NNRSTVFRIV PKFKKEKVQK 1150 1160 QKTSSQPGSG DTESGSCEAN SPGN
	Isoform 3 [UniParc]. Checksum: 0D36C1FA1E7C1A43	FASTA	1,100	122,392
	10 20 30 40 50 60 MLCAENRKEM EDWISSLKSV QTREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL 70 80 90 100 110 120 SGVTSHGLSC EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP 130 140 150 160 170 180 VSAKCAVCDK TCGSVLRLQD WKCLWCKTMV HTACKDLYHP ICPLGQCKVS IIPPIALNST 190 200 210 220 230 240 DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL LNPAQVFDLM NGGPHLGLRL 250 260 270 280 290 300 FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL NKQCQLGVLP LGTGNDLARV LGWGGSYDDD 310 320 330 340 350 360 TQLPQILEKL ERASTKMLDR WSIMTYELKL PPKASLLPGP PEASEEFYMT IYEDSVATHL 370 380 390 400 410 420 TKILNSDEHA VVISSAKTLC ETVKDFVAKV EKTYDKTLEN AVVADAVASK CSVLNEKLEQ 430 440 450 460 470 480 LLQALHTDSQ AAPVLPGLSP LIVEEDAVES SSEESLGESK EQLGDDVTKP SSQKAVKPRE 490 500 510 520 530 540 IMLRANSLKK AVRQVIEEAG KVMDDPTVHP CEPANQSSDY DSTETDESKE EAKDDGAKES 550 560 570 580 590 600 ITVKTAPRSP DARASYGHSQ TDSVPGPAVA ASKENLPVLN TRIICPGLRA GLAASIAGSS 610 620 630 640 650 660 IINKMLLANI DPFGATPFID PDLDSVDGYS EKCVMNNYFG IGLDAKISLE FNNKREEHPE 670 680 690 700 710 720 KCRSRTKNLM WYGVLGTREL LQRSYKNLEQ RVQLECDGQY IPLPSLQGIA VLNIPSYAGG 730 740 750 760 770 780 TNFWGGTKED DIFAAPSFDD KILEVVAIFD SMQMAVSRVI KLQHHRIAQC RTVKITIFGD 790 800 810 820 830 840 EGVPVQVDGE AWVQPPGIIK IVHKNRAQML TRDRAFESTL KSWEDKQKCD SGKPVLRTHL 850 860 870 880 890 900 YIHHAIDLAT EEVSQMQLCS QAAEELITRI CDAATIHCLL EQELAHAVNA CSHALNKANP 910 920 930 940 950 960 RCPESLTRDT ATEIAINVKA LYNETESLLV GRVPLQLESP HEERVSNALH SVEVELQKLT 970 980 990 1000 1010 1020 EIPWLYYILH PNEDEEPPMD CTKRNNRSTV FRIVPKFKKE KVQKQKTSSQ PVQKWGTEEV 1030 1040 1050 1060 1070 1080 AAWLDLLNLG EYKDIFIRHD IRGAELLHLE RRDLKNTVGE KRDTKENGKH MDLGIPKVGH 1090 1100 VKRILQGIKE LGRSTPQSEV
	Isoform 4 [UniParc]. Checksum: 8994FBC10F6DB99D	FASTA	1,084	120,566
	10 20 30 40 50 60 MLCAENRKEM EDWISSLKSV QTREPYEVAQ FNVEHFSGMH NWYACSHARP TFCNVCRESL 70 80 90 100 110 120 SGVTSHGLSC EVCKFKAHKR CAVRATNNCK WTTLASIGKD IIEDEDGVAM PHQWLEGNLP 130 140 150 160 170 180 VSAKCAVCDK TCGSVLRLQD WKCLWCKTMV HTACKDLYHP ICPLGQCKVS IIPPIALNST 190 200 210 220 230 240 DSDGFCRATF SFCVSPLLVF VNSKSGDNQG VKFLRRFKQL LNPAQVFDLM NGGPHLGLRL 250 260 270 280 290 300 FQKFDNFRIL VCGGDGSVGW VLSEIDKLNL NKQCQLGVLP LGTGNDLARV LGWGGSYDDD 310 320 330 340 350 360 TQLPQILEKL ERASTKMLDR WSIMTYELKL PPKASLLPGP PEASEEFYMT IYEDSVATHL 370 380 390 400 410 420 TKILNSDEHA VVISSAKTLC ETVKDFVAKV EKTYDKTLEN AVVADAVASK CSVLNEKLEQ 430 440 450 460 470 480 LLQALHTDSQ AAPVLPGLSP LIVEEDAVES SSEESLGESK EQLGDDVTKP SSQKAVKPRE 490 500 510 520 530 540 IMLRANSLKK AVRQVIEEAG KVMDDPTVHP CEPANQSSDY DSTETDESKE EAKDDGAKES 550 560 570 580 590 600 ITVKTAPRSP DARASYGHSQ TDSVPGPAVA ASKENLPVLN TRIICPGLRA GLAASIAGSS 610 620 630 640 650 660 IINKMLLANI DPFGATPFID PDLDSVDGYS EKCVMNNYFG IGLDAKISLE FNNKREEHPE 670 680 690 700 710 720 KCRSRTKNLM WYGVLGTREL LQRSYKNLEQ RVQLECDGQY IPLPSLQGIA VLNIPSYAGG 730 740 750 760 770 780 TNFWGGTKED DIFAAPSFDD KILEVVAIFD SMQMAVSRVI KLQHHRIAQC RTVKITIFGD 790 800 810 820 830 840 EGVPVQVDGE AWVQPPGIIK IVHKNRAQML TRDRAFESTL KSWEDKQKCD SGKPVLRTHL 850 860 870 880 890 900 YIHHAIDLAT EEVSQMQLCS QAAEELITRI CDAATIHCLL EQELAHAVNA CSHALNKANP 910 920 930 940 950 960 RCPESLTRDT ATEIAINVKA LYNETESLLV GRVPLQLESP HEERVSNALH SVEVELQKLT 970 980 990 1000 1010 1020 EIPWLYYILH PNEDEEPPMD CTKRNNRSTV FRIVPKFKKE KVQKQKTSSQ PVQKWGTEEV 1030 1040 1050 1060 1070 1080 AAWLDLLNLG EYKDIFIRHD IRGAELLHLE RRDLKDLGIP KVGHVKRILQ GIKELGRSTP QSEV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of two splice variants of human diacylglycerol kinase eta." Murakami T., Sakane F., Imai S., Houkin K., Kanoh H. J. Biol. Chem. 278:34364-34372(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION, MUTAGENESIS OF TRP-1151. Tissue: Testis.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 640-1220 (ISOFORM 2). Tissue: Testis and Uterus.
[3]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Testis.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 617-1220 (ISOFORM 1). Tissue: Amygdala.
[6]	"Diacylglycerol kinase eta augments C-Raf activity and B-Raf/C-Raf heterodimerization." Yasuda S., Kai M., Imai S., Takeishi K., Taketomi A., Toyota M., Kanoh H., Sakane F. J. Biol. Chem. 284:29559-29570(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RAF1 AND BRAF.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB078967 mRNA. Translation: BAC66960.1. AB078968 mRNA. Translation: BAC66961.1. AK302727 mRNA. Translation: BAG63946.1. AL136527, AL139328, AL157932 Genomic DNA. Translation: CAH71399.2. AL136527, AL139328, AL157932 Genomic DNA. Translation: CAM14245.1. AL139328, AL136527, AL157932 Genomic DNA. Translation: CAI16894.2. AL139328, AL136527, AL157932 Genomic DNA. Translation: CAM23676.1. AL157932, AL136527, AL139328 Genomic DNA. Translation: CAH71062.2. AL157932, AL136527, AL139328 Genomic DNA. Translation: CAM13770.1. BC043292 mRNA. Translation: AAH43292.1. AL833883 mRNA. Translation: CAD38739.1. AK098302 mRNA. Translation: BAC05280.1. Different initiation.
IPI	IPI00329264. IPI00745381. IPI00759622.
RefSeq	NP_001191433.1. NM_001204504.1. NP_001191434.1. NM_001204505.1. NP_001191435.1. NM_001204506.1. NP_690874.2. NM_152910.4. NP_821077.1. NM_178009.3.
UniGene	Hs.733239.
3D structure databases
ProteinModelPortal	Q86XP1.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86XP1. 2 interactions.
STRING	9606.ENSP00000337572.
PTM databases
PhosphoSite	Q86XP1.
Polymorphism databases
DMDM	74762463.
Proteomic databases
PaxDb	Q86XP1.
PRIDE	Q86XP1.
Protocols and materials databases
DNASU	160851.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261491; ENSP00000261491; ENSG00000102780. ENST00000337343; ENSP00000337572; ENSG00000102780. ENST00000379274; ENSP00000368576; ENSG00000102780. ENST00000536612; ENSP00000445114; ENSG00000102780. ENST00000540693; ENSP00000440823; ENSG00000102780.
GeneID	160851.
KEGG	hsa:160851.
UCSC	uc001uyl.2. human. uc001uym.2. human. uc001uyo.2. human.
Organism-specific databases
CTD	160851.
GeneCards	GC13P042622.
HGNC	HGNC:2854. DGKH.
HPA	HPA039533. HPA040355.
MIM	604071. gene.
neXtProt	NX_Q86XP1.
PharmGKB	PA27315.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG328046.
HOVERGEN	HBG051346.
InParanoid	Q86XP1.
KO	K00901.
OMA	EEFYMTI.
PhylomeDB	Q86XP1.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	Q86XP1.
Bgee	Q86XP1.
CleanEx	HS_DGKH.
Genevestigator	Q86XP1.
GermOnline	ENSG00000102780. Homo sapiens.
Family and domain databases
Gene3D	1.10.150.50. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF07647. SAM_2. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	DGKH. human.
GenomeRNAi	160851.
NextBio	88001.
SOURCE	Search...

Entry information

Entry name

DGKH_HUMAN

Accession

Primary (citable) accession number: Q86XP1
Secondary accession number(s): A2A2W7 , A6NFX7, B4DZ34, Q5VZW0, Q6PI56, Q86XP2, Q8N3N0, Q8N7J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.