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Q86XP0 (PA24D_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic phospholipase A2 delta

Short name=cPLA2-delta
EC=3.1.1.4
Alternative name(s):
Phospholipase A2 group IVD
Gene names
Name:PLA2G4D
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length818 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Not arachidonic acid-specific but has linoleic acid-specific activity. May play a role in inflammation in psoriatic lesions. Ref.1

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulation

Stimulated by cytosolic Ca2+. Ref.1

Subcellular location

Cytoplasmcytosol. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Translocates to membrane vesicles in a calcium-dependent fashion. Translocates to perinuclear regions upon ionomycin stimulation By similarity.

Tissue specificity

Expressed in stratified squamous epithelia, such as those in skin and cervix, but not in other tissues. Strongly expressed in the upper spinous layer of the psoriatic epidermis, expressed weakly and discontinuously in atopic dermatitis and mycosis fungoides, and not detected in the epidermis of normal skin. Ref.1

Domain

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.

Sequence similarities

Contains 1 C2 domain.

Contains 1 PLA2c domain.

Sequence caution

The sequence AAH34571.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86XP0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86XP0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     682-723: ALQQTELYCR...HLFSDPACPE → VPWSPQGNPS...VPKGRRGVRP
     724-818: Missing.
Note: May be due to an intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 818818Cytosolic phospholipase A2 delta
PRO_0000247023

Regions

Domain9 – 120112C2
Domain273 – 818546PLA2c

Sites

Active site3611Nucleophile By similarity
Active site6471Proton acceptor By similarity

Natural variations

Alternative sequence682 – 72342ALQQT…PACPE → VPWSPQGNPSAQPGQAPEAS SRATEPLPHTAGVPKGRRGV RP in isoform 2.
VSP_019881
Alternative sequence724 – 81895Missing in isoform 2.
VSP_019882
Natural variant2751P → R.
Corresponds to variant rs11635685 [ dbSNP | Ensembl ].
VAR_057676
Natural variant4341S → T.
Corresponds to variant rs4924618 [ dbSNP | Ensembl ].
VAR_057677
Natural variant5731R → W.
Corresponds to variant rs17747505 [ dbSNP | Ensembl ].
VAR_027049
Natural variant6491A → G.
Corresponds to variant rs17690899 [ dbSNP | Ensembl ].
VAR_027050
Natural variant7471R → G. Ref.4
Corresponds to variant rs2459692 [ dbSNP | Ensembl ].
VAR_027051
Natural variant7831R → Q.
Corresponds to variant rs750052 [ dbSNP | Ensembl ].
VAR_057678
Natural variant8071R → Q.
Corresponds to variant rs750051 [ dbSNP | Ensembl ].
VAR_057679

Experimental info

Sequence conflict101P → T in BAC67158. Ref.1
Isoform 2:
Sequence conflict7131G → R in AAH34571. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: 06187324748A4C21

FASTA81891,952
        10         20         30         40         50         60 
MESLSPGGPP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ LSTAPGMKFK 

        70         80         90        100        110        120 
TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE DDICFKVLYD ISEVLPGKLL 

       130        140        150        160        170        180 
RKTFSQSPQG EEELDVEFLM EETSDRPENL ITNKVIVARE LSCLDVHLDS TGSTAVVADQ 

       190        200        210        220        230        240 
DKLELELVLK GSYEDTQTSF LGTASAFRFH YMAALETELS GRLRSSRSNG WNGDNSAGYL 

       250        260        270        280        290        300 
TVPLRPLTIG KEVTMDVPAP NAPGVRLQLK AEGCPEELAV HLGFNLCAEE QAFLSRRKQV 

       310        320        330        340        350        360 
VAKALKQALQ LDRDLQEDEV PVVGIMATGG GARAMTSLYG HLLALQKLGL LDCVTYFSGI 

       370        380        390        400        410        420 
SGSTWTMAHL YGDPEWSQRD LEGPIRYARE HLAKSKLEVF SPERLASYRR ELELRAEQGH 

       430        440        450        460        470        480 
PTTFVDLWAL VLESMLHGQV MDQKLSGQRA ALERGQNPLP LYLSLNVKEN NLETLDFKEW 

       490        500        510        520        530        540 
VEFSPYEVGF LKYGAFVPPE LFGSEFFMGR LMRRIPEPRI CFLEAIWSNI FSLNLLDAWY 

       550        560        570        580        590        600 
DLTSSGESWK QHIKDKTRSL EKEPLTTSGT SSRLEASWLQ PGTALAQAFK GFLTGRPLHQ 

       610        620        630        640        650        660 
RSPNFLQGLQ LHQDYCSHKD FSTWADYQLD SMPSQLTPKE PRLCLVDAAY FINTSSPSMF 

       670        680        690        700        710        720 
RPGRRLDLIL SFDYSLSAPF EALQQTELYC RARGLPFPRV EPSPQDQHQP RECHLFSDPA 

       730        740        750        760        770        780 
CPEAPILLHF PLVNASFKDH SAPGVQRSPA ELQGGQVDLT GATCPYTLSN MTYKEEDFER 

       790        800        810 
LLRLSDYNVQ TSQGAILQAL RTALKHRTLE ARPPRAQT 

« Hide

Isoform 2 [UniParc].

Checksum: 0C385309D4BAB085
Show »

FASTA72380,930

References

« Hide 'large scale' references
[1]"Cloning of a gene for a novel epithelium-specific cytosolic phospholipase A2, cPLA2delta, induced in psoriatic skin."
Chiba H., Michibata H., Wakimoto K., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
J. Biol. Chem. 279:12890-12897(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.
[2]"Analysis of the DNA sequence and duplication history of human chromosome 15."
Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. expand/collapse author list , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 491-818 (ISOFORM 2).
Tissue: Brain.
[4]"A family based study of the genetic association between the PLA2G4D gene and schizophrenia."
Tao R., Yu Y., Zhang X., Shi J., Guo Y., Wang C., Han B., Xu Q., Shang H., Zhang X., Xie L., Liu S., Ju G., Shen Y., Wei J.
Prostaglandins Leukot. Essent. Fatty Acids 73:419-422(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT GLY-747.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB090876 mRNA. Translation: BAC67158.1.
AC084693 Genomic DNA. No translation available.
BC034571 mRNA. Translation: AAH34571.1. Different initiation.
RefSeqNP_828848.3. NM_178034.3.
UniGeneHs.380225.

3D structure databases

ProteinModelPortalQ86XP0.
SMRQ86XP0. Positions 17-809.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid129664. 1 interaction.
STRING9606.ENSP00000290472.

PTM databases

PhosphoSiteQ86XP0.

Polymorphism databases

DMDM269849641.

Proteomic databases

PaxDbQ86XP0.
PRIDEQ86XP0.

Protocols and materials databases

DNASU283748.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000290472; ENSP00000290472; ENSG00000159337. [Q86XP0-1]
GeneID283748.
KEGGhsa:283748.
UCSCuc001zox.3. human. [Q86XP0-1]

Organism-specific databases

CTD283748.
GeneCardsGC15M042359.
H-InvDBHIX0026772.
HGNCHGNC:30038. PLA2G4D.
HPAHPA039489.
MIM612864. gene.
neXtProtNX_Q86XP0.
PharmGKBPA134974074.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG300348.
HOGENOMHOG000231788.
HOVERGENHBG080412.
InParanoidQ86XP0.
KOK16342.
OMATYKEEDF.
OrthoDBEOG70CR62.
PhylomeDBQ86XP0.
TreeFamTF325228.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

BgeeQ86XP0.
CleanExHS_PLA2G4D.
GenevestigatorQ86XP0.

Family and domain databases

InterProIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi283748.
NextBio94215.
PROQ86XP0.
SOURCESearch...

Entry information

Entry namePA24D_HUMAN
AccessionPrimary (citable) accession number: Q86XP0
Secondary accession number(s): Q8N176
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 24, 2009
Last modified: March 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM