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Q86XP0

- PA24D_HUMAN

UniProt

Q86XP0 - PA24D_HUMAN

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Protein

Cytosolic phospholipase A2 delta

Gene

PLA2G4D

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Not arachidonic acid-specific but has linoleic acid-specific activity. May play a role in inflammation in psoriatic lesions.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by cytosolic Ca2+.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei361 – 3611NucleophileBy similarity
Active sitei647 – 6471Proton acceptorBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerophospholipid biosynthetic process Source: Reactome
  2. phosphatidic acid biosynthetic process Source: Reactome
  3. phosphatidylcholine acyl-chain remodeling Source: Reactome
  4. phosphatidylethanolamine acyl-chain remodeling Source: Reactome
  5. phosphatidylglycerol acyl-chain remodeling Source: Reactome
  6. phosphatidylinositol acyl-chain remodeling Source: Reactome
  7. phosphatidylserine acyl-chain remodeling Source: Reactome
  8. phospholipid catabolic process Source: InterPro
  9. phospholipid metabolic process Source: Reactome
  10. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120906. Synthesis of PA.
REACT_120977. Hydrolysis of LPC.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2 delta (EC:3.1.1.4)
Short name:
cPLA2-delta
Alternative name(s):
Phospholipase A2 group IVD
Gene namesi
Name:PLA2G4D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 15

Organism-specific databases

HGNCiHGNC:30038. PLA2G4D.

Subcellular locationi

Cytoplasmcytosol. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion. Translocates to perinuclear regions upon ionomycin stimulation (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. cytosol Source: Reactome
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134974074.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 818818Cytosolic phospholipase A2 deltaPRO_0000247023Add
BLAST

Proteomic databases

MaxQBiQ86XP0.
PaxDbiQ86XP0.
PRIDEiQ86XP0.

PTM databases

PhosphoSiteiQ86XP0.

Expressioni

Tissue specificityi

Expressed in stratified squamous epithelia, such as those in skin and cervix, but not in other tissues. Strongly expressed in the upper spinous layer of the psoriatic epidermis, expressed weakly and discontinuously in atopic dermatitis and mycosis fungoides, and not detected in the epidermis of normal skin.1 Publication

Gene expression databases

BgeeiQ86XP0.
CleanExiHS_PLA2G4D.
GenevestigatoriQ86XP0.

Organism-specific databases

HPAiHPA039489.

Interactioni

Protein-protein interaction databases

BioGridi129664. 1 interaction.
STRINGi9606.ENSP00000290472.

Structurei

3D structure databases

ProteinModelPortaliQ86XP0.
SMRiQ86XP0. Positions 17-807.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 120112C2PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 818546PLA2cPROSITE-ProRule annotationAdd
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ (By similarity).By similarity

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG300348.
GeneTreeiENSGT00550000074489.
HOGENOMiHOG000231788.
HOVERGENiHBG080412.
InParanoidiQ86XP0.
KOiK16342.
OMAiTYKEEDF.
OrthoDBiEOG70CR62.
PhylomeDBiQ86XP0.
TreeFamiTF325228.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q86XP0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESLSPGGPP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ
60 70 80 90 100
LSTAPGMKFK TKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE
110 120 130 140 150
DDICFKVLYD ISEVLPGKLL RKTFSQSPQG EEELDVEFLM EETSDRPENL
160 170 180 190 200
ITNKVIVARE LSCLDVHLDS TGSTAVVADQ DKLELELVLK GSYEDTQTSF
210 220 230 240 250
LGTASAFRFH YMAALETELS GRLRSSRSNG WNGDNSAGYL TVPLRPLTIG
260 270 280 290 300
KEVTMDVPAP NAPGVRLQLK AEGCPEELAV HLGFNLCAEE QAFLSRRKQV
310 320 330 340 350
VAKALKQALQ LDRDLQEDEV PVVGIMATGG GARAMTSLYG HLLALQKLGL
360 370 380 390 400
LDCVTYFSGI SGSTWTMAHL YGDPEWSQRD LEGPIRYARE HLAKSKLEVF
410 420 430 440 450
SPERLASYRR ELELRAEQGH PTTFVDLWAL VLESMLHGQV MDQKLSGQRA
460 470 480 490 500
ALERGQNPLP LYLSLNVKEN NLETLDFKEW VEFSPYEVGF LKYGAFVPPE
510 520 530 540 550
LFGSEFFMGR LMRRIPEPRI CFLEAIWSNI FSLNLLDAWY DLTSSGESWK
560 570 580 590 600
QHIKDKTRSL EKEPLTTSGT SSRLEASWLQ PGTALAQAFK GFLTGRPLHQ
610 620 630 640 650
RSPNFLQGLQ LHQDYCSHKD FSTWADYQLD SMPSQLTPKE PRLCLVDAAY
660 670 680 690 700
FINTSSPSMF RPGRRLDLIL SFDYSLSAPF EALQQTELYC RARGLPFPRV
710 720 730 740 750
EPSPQDQHQP RECHLFSDPA CPEAPILLHF PLVNASFKDH SAPGVQRSPA
760 770 780 790 800
ELQGGQVDLT GATCPYTLSN MTYKEEDFER LLRLSDYNVQ TSQGAILQAL
810
RTALKHRTLE ARPPRAQT
Length:818
Mass (Da):91,952
Last modified:November 24, 2009 - v2
Checksum:i06187324748A4C21
GO
Isoform 2 (identifier: Q86XP0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     682-723: ALQQTELYCR...HLFSDPACPE → VPWSPQGNPS...VPKGRRGVRP
     724-818: Missing.

Note: May be due to an intron retention.Curated

Show »
Length:723
Mass (Da):80,930
Checksum:i0C385309D4BAB085
GO

Sequence cautioni

The sequence AAH34571.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101P → T in BAC67158. (PubMed:14709560)Curated
Isoform 2 (identifier: Q86XP0-2)
Sequence conflicti713 – 7131G → R in AAH34571. (PubMed:16572171)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti275 – 2751P → R.
Corresponds to variant rs11635685 [ dbSNP | Ensembl ].
VAR_057676
Natural varianti434 – 4341S → T.
Corresponds to variant rs4924618 [ dbSNP | Ensembl ].
VAR_057677
Natural varianti573 – 5731R → W.
Corresponds to variant rs17747505 [ dbSNP | Ensembl ].
VAR_027049
Natural varianti649 – 6491A → G.
Corresponds to variant rs17690899 [ dbSNP | Ensembl ].
VAR_027050
Natural varianti747 – 7471R → G.1 Publication
Corresponds to variant rs2459692 [ dbSNP | Ensembl ].
VAR_027051
Natural varianti783 – 7831R → Q.
Corresponds to variant rs750052 [ dbSNP | Ensembl ].
VAR_057678
Natural varianti807 – 8071R → Q.
Corresponds to variant rs750051 [ dbSNP | Ensembl ].
VAR_057679

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei682 – 72342ALQQT…PACPE → VPWSPQGNPSAQPGQAPEAS SRATEPLPHTAGVPKGRRGV RP in isoform 2. 1 PublicationVSP_019881Add
BLAST
Alternative sequencei724 – 81895Missing in isoform 2. 1 PublicationVSP_019882Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB090876 mRNA. Translation: BAC67158.1.
AC084693 Genomic DNA. No translation available.
BC034571 mRNA. Translation: AAH34571.1. Different initiation.
CCDSiCCDS32203.1. [Q86XP0-1]
RefSeqiNP_828848.3. NM_178034.3. [Q86XP0-1]
UniGeneiHs.380225.

Genome annotation databases

EnsembliENST00000290472; ENSP00000290472; ENSG00000159337. [Q86XP0-1]
GeneIDi283748.
KEGGihsa:283748.
UCSCiuc001zox.3. human. [Q86XP0-1]

Polymorphism databases

DMDMi269849641.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB090876 mRNA. Translation: BAC67158.1 .
AC084693 Genomic DNA. No translation available.
BC034571 mRNA. Translation: AAH34571.1 . Different initiation.
CCDSi CCDS32203.1. [Q86XP0-1 ]
RefSeqi NP_828848.3. NM_178034.3. [Q86XP0-1 ]
UniGenei Hs.380225.

3D structure databases

ProteinModelPortali Q86XP0.
SMRi Q86XP0. Positions 17-807.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 129664. 1 interaction.
STRINGi 9606.ENSP00000290472.

PTM databases

PhosphoSitei Q86XP0.

Polymorphism databases

DMDMi 269849641.

Proteomic databases

MaxQBi Q86XP0.
PaxDbi Q86XP0.
PRIDEi Q86XP0.

Protocols and materials databases

DNASUi 283748.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000290472 ; ENSP00000290472 ; ENSG00000159337 . [Q86XP0-1 ]
GeneIDi 283748.
KEGGi hsa:283748.
UCSCi uc001zox.3. human. [Q86XP0-1 ]

Organism-specific databases

CTDi 283748.
GeneCardsi GC15M042359.
H-InvDB HIX0026772.
HGNCi HGNC:30038. PLA2G4D.
HPAi HPA039489.
MIMi 612864. gene.
neXtProti NX_Q86XP0.
PharmGKBi PA134974074.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG300348.
GeneTreei ENSGT00550000074489.
HOGENOMi HOG000231788.
HOVERGENi HBG080412.
InParanoidi Q86XP0.
KOi K16342.
OMAi TYKEEDF.
OrthoDBi EOG70CR62.
PhylomeDBi Q86XP0.
TreeFami TF325228.

Enzyme and pathway databases

Reactomei REACT_120722. Acyl chain remodelling of PI.
REACT_120829. Acyl chain remodelling of PC.
REACT_120906. Synthesis of PA.
REACT_120977. Hydrolysis of LPC.
REACT_121324. Acyl chain remodelling of PG.
REACT_121369. Acyl chain remodelling of PE.
REACT_121384. Acyl chain remodelling of PS.

Miscellaneous databases

GenomeRNAii 283748.
NextBioi 94215.
PROi Q86XP0.
SOURCEi Search...

Gene expression databases

Bgeei Q86XP0.
CleanExi HS_PLA2G4D.
Genevestigatori Q86XP0.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a gene for a novel epithelium-specific cytosolic phospholipase A2, cPLA2delta, induced in psoriatic skin."
    Chiba H., Michibata H., Wakimoto K., Seishima M., Kawasaki S., Okubo K., Mitsui H., Torii H., Imai Y.
    J. Biol. Chem. 279:12890-12897(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, ENZYME REGULATION, TISSUE SPECIFICITY.
  2. "Analysis of the DNA sequence and duplication history of human chromosome 15."
    Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A.
    , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.
    Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 491-818 (ISOFORM 2).
    Tissue: Brain.
  4. "A family based study of the genetic association between the PLA2G4D gene and schizophrenia."
    Tao R., Yu Y., Zhang X., Shi J., Guo Y., Wang C., Han B., Xu Q., Shang H., Zhang X., Xie L., Liu S., Ju G., Shen Y., Wei J.
    Prostaglandins Leukot. Essent. Fatty Acids 73:419-422(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT GLY-747.

Entry informationi

Entry nameiPA24D_HUMAN
AccessioniPrimary (citable) accession number: Q86XP0
Secondary accession number(s): Q8N176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 24, 2009
Last modified: October 29, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3