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Q86XN8

- MEX3D_HUMAN

UniProt

Q86XN8 - MEX3D_HUMAN

Protein

RNA-binding protein MEX3D

Gene

MEX3D

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (20 Mar 2007)
      Previous versions | rss
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    Functioni

    RNA binding protein, may be involved in post-transcriptional regulatory mechanisms.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 64041RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. AU-rich element binding Source: BHF-UCL
    2. poly(A) RNA binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mRNA destabilization Source: BHF-UCL
    2. mRNA localization resulting in posttranscriptional regulation of gene expression Source: BHF-UCL

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-binding protein MEX3D
    Alternative name(s):
    RING finger and KH domain-containing protein 1
    RING finger protein 193
    TINO
    Gene namesi
    Name:MEX3D
    Synonyms:KIAA2031, RKHD1, RNF193
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:16734. MEX3D.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Predominantly expressed in the cytoplasm and shuttles between the cytoplasm and the nucleus through the CRM1 export pathway.By similarity

    GO - Cellular componenti

    1. nucleus Source: BHF-UCL
    2. perinuclear region of cytoplasm Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162395829.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 651651RNA-binding protein MEX3DPRO_0000050123Add
    BLAST

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ86XN8.
    PaxDbiQ86XN8.
    PRIDEiQ86XN8.

    PTM databases

    PhosphoSiteiQ86XN8.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in all the cell lines and tissues tested.1 Publication

    Gene expression databases

    ArrayExpressiQ86XN8.
    BgeeiQ86XN8.
    CleanExiHS_MEX3D.
    GenevestigatoriQ86XN8.

    Interactioni

    Protein-protein interaction databases

    BioGridi134376. 6 interactions.
    STRINGi9606.ENSP00000384398.

    Structurei

    Secondary structure

    1
    651
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi273 – 2797
    Helixi282 – 2898
    Turni290 – 2934
    Helixi296 – 3027
    Beta strandi306 – 3083
    Beta strandi312 – 3143
    Beta strandi317 – 3226
    Turni324 – 3263
    Helixi327 – 33913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DGRNMR-A272-341[»]
    ProteinModelPortaliQ86XN8.
    SMRiQ86XN8. Positions 273-343, 598-644.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ86XN8.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini179 – 24062KH 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini273 – 33462KH 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Binds RNA through its KH domains.By similarity

    Sequence similaritiesi

    Contains 2 KH domains.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri600 – 64041RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG260228.
    HOGENOMiHOG000236282.
    HOVERGENiHBG071698.
    KOiK15686.
    OMAiSLWAKTP.
    OrthoDBiEOG7D85WG.
    PhylomeDBiQ86XN8.
    TreeFamiTF315107.

    Family and domain databases

    Gene3Di3.30.1370.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF00013. KH_1. 2 hits.
    [Graphical view]
    SMARTiSM00322. KH. 2 hits.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF54791. SSF54791. 2 hits.
    PROSITEiPS50084. KH_TYPE_1. 2 hits.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86XN8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPSSLGQPDG GGGGGGGGGG VGAAGEDPGP GPAPPPEGAQ EAAPAPRPPP    50
    EPDDAAAALR LALDQLSALG LGGAGDTDEE GAAGDGAAAA GGADGGAAPE 100
    PVPPDGPEAG APPTLAPAVA PGSLPLLDPN ASPPPPPPPR PSPPDVFAGF 150
    APHPAALGPP TLLADQMSVI GSRKKSVNMT ECVPVPSSEH VAEIVGRQGC 200
    KIKALRAKTN TYIKTPVRGE EPVFIVTGRK EDVEMAKREI LSAAEHFSII 250
    RATRSKAGGL PGAAQGPPNL PGQTTIQVRV PYRVVGLVVG PKGATIKRIQ 300
    QRTHTYIVTP GRDKEPVFAV TGMPENVDRA REEIEAHITL RTGAFTDAGP 350
    DSDFHANGTD VCLDLLGAAA SLWAKTPNQG RRPPTATAGL RGDTALGAPS 400
    APEAFYAGSR GGPSVPDPGP ASPYSGSGNG GFAFGAEGPG APVGTAAPDD 450
    CDFGFDFDFL ALDLTVPAAA TIWAPFERAA PLPAFSGCST VNGAPGPPAA 500
    GARRSSGAGT PRHSPTLPEP GGLRLELPLS RRGAPDPVGA LSWRPPQGPV 550
    SFPGGAAFST ATSLPSSPAA AACAPLDSGA SENSRKPPSA SSAPALAREC 600
    VVCAEGEVMA ALVPCGHNLF CMDCAVRICG KSEPECPACR TPATQAIHIF 650
    S 651
    Length:651
    Mass (Da):64,883
    Last modified:March 20, 2007 - v3
    Checksum:i5E056EE2D08BC4CD
    GO
    Isoform 2 (identifier: Q86XN8-2) [UniParc]FASTAAdd to Basket

    Also known as: TINO

    The sequence of this isoform differs from the canonical sequence as follows:
         648-651: HIFS → RVETPQPGGASALQRQY

    Show »
    Length:664
    Mass (Da):66,238
    Checksum:iCA8D41D3EEFD3A48
    GO
    Isoform 3 (identifier: Q86XN8-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         648-651: HIFS → RVETETPQPGGASALQRQY

    Note: No experimental confirmation available.

    Show »
    Length:666
    Mass (Da):66,469
    Checksum:i498479459CECBD6C
    GO

    Sequence cautioni

    The sequence AAQ04763.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei648 – 6514HIFS → RVETPQPGGASALQRQY in isoform 2. 2 PublicationsVSP_024019
    Alternative sequencei648 – 6514HIFS → RVETETPQPGGASALQRQY in isoform 3. CuratedVSP_047662

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY950680 mRNA. Translation: AAY34148.1.
    AC005943 Genomic DNA. No translation available.
    AC027307 Genomic DNA. No translation available.
    AB107353 mRNA. Translation: BAC67658.1.
    AF458084 mRNA. Translation: AAQ04763.1. Different initiation.
    BC113384 mRNA. Translation: AAI13385.1.
    BC113741 mRNA. Translation: AAI13742.2.
    CCDSiCCDS32865.2. [Q86XN8-1]
    RefSeqiNP_001167589.1. NM_001174118.1. [Q86XN8-3]
    NP_976049.3. NM_203304.3. [Q86XN8-1]
    UniGeneiHs.436495.

    Genome annotation databases

    EnsembliENST00000402693; ENSP00000384398; ENSG00000181588. [Q86XN8-1]
    GeneIDi399664.
    KEGGihsa:399664.

    Polymorphism databases

    DMDMi134047829.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY950680 mRNA. Translation: AAY34148.1 .
    AC005943 Genomic DNA. No translation available.
    AC027307 Genomic DNA. No translation available.
    AB107353 mRNA. Translation: BAC67658.1 .
    AF458084 mRNA. Translation: AAQ04763.1 . Different initiation.
    BC113384 mRNA. Translation: AAI13385.1 .
    BC113741 mRNA. Translation: AAI13742.2 .
    CCDSi CCDS32865.2. [Q86XN8-1 ]
    RefSeqi NP_001167589.1. NM_001174118.1. [Q86XN8-3 ]
    NP_976049.3. NM_203304.3. [Q86XN8-1 ]
    UniGenei Hs.436495.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DGR NMR - A 272-341 [» ]
    ProteinModelPortali Q86XN8.
    SMRi Q86XN8. Positions 273-343, 598-644.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 134376. 6 interactions.
    STRINGi 9606.ENSP00000384398.

    PTM databases

    PhosphoSitei Q86XN8.

    Polymorphism databases

    DMDMi 134047829.

    Proteomic databases

    MaxQBi Q86XN8.
    PaxDbi Q86XN8.
    PRIDEi Q86XN8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000402693 ; ENSP00000384398 ; ENSG00000181588 . [Q86XN8-1 ]
    GeneIDi 399664.
    KEGGi hsa:399664.

    Organism-specific databases

    CTDi 399664.
    GeneCardsi GC19M001554.
    HGNCi HGNC:16734. MEX3D.
    MIMi 611009. gene.
    neXtProti NX_Q86XN8.
    PharmGKBi PA162395829.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG260228.
    HOGENOMi HOG000236282.
    HOVERGENi HBG071698.
    KOi K15686.
    OMAi SLWAKTP.
    OrthoDBi EOG7D85WG.
    PhylomeDBi Q86XN8.
    TreeFami TF315107.

    Miscellaneous databases

    EvolutionaryTracei Q86XN8.
    GeneWikii MEX3D.
    GenomeRNAii 399664.
    NextBioi 105463.
    PROi Q86XN8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86XN8.
    Bgeei Q86XN8.
    CleanExi HS_MEX3D.
    Genevestigatori Q86XN8.

    Family and domain databases

    Gene3Di 3.30.1370.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF00013. KH_1. 2 hits.
    [Graphical view ]
    SMARTi SM00322. KH. 2 hits.
    SM00184. RING. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54791. SSF54791. 2 hits.
    PROSITEi PS50084. KH_TYPE_1. 2 hits.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies."
      Buchet-Poyau K., Courchet J., Le Hir H., Seraphin B., Scoazec J.-Y., Duret L., Domon-Dell C., Freund J.-N., Billaud M.
      Nucleic Acids Res. 35:1289-1300(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The nucleotide sequence of a long cDNA clone isolated from human."
      Nagase T., Kikuno R., Ohara O.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 137-651 (ISOFORM 1).
      Tissue: Brain.
    4. "Identification of TINO: a new evolutionarily conserved BCL-2 AU-rich element RNA-binding protein."
      Donnini M., Lapucci A., Papucci L., Witort E., Jacquier A., Brewer G., Nicolin A., Capaccioli S., Schiavone N.
      J. Biol. Chem. 279:20154-20166(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-651 (ISOFORM 2).
      Tissue: Placenta.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 178-651 (ISOFORM 2).
    6. "Solution structure of the second KH domain in RING finger and KH domain-containing protein 1."
      RIKEN structural genomics initiative (RSGI)
      Submitted (SEP-2006) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 272-343.

    Entry informationi

    Entry nameiMEX3D_HUMAN
    AccessioniPrimary (citable) accession number: Q86XN8
    Secondary accession number(s): A0PJL8
    , A1L023, E9PAL6, Q71M49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: March 20, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3