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Protein

Protein phosphatase 1 regulatory subunit 3B

Gene

PPP1R3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name:
PP1 subunit R4
Protein phosphatase 1 subunit GL
Short name:
PTG
Gene namesi
Name:PPP1R3B
Synonyms:PPP1R4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:14942. PPP1R3B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33652.

Polymorphism and mutation databases

BioMutaiPPP1R3B.
DMDMi74727837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Protein phosphatase 1 regulatory subunit 3BPRO_0000324543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei261 – 2611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ86XI6.
PaxDbiQ86XI6.
PRIDEiQ86XI6.

PTM databases

iPTMnetiQ86XI6.
PhosphoSiteiQ86XI6.

Expressioni

Tissue specificityi

Highly expressed in the liver and, at lower levels, in skeletal muscle, including in vastus lateralis, gastrocnemius and soleus (at protein level). Highest mRNA levels are observed in skeletal muscle, and only moderate levels in liver and heart. Weak expression in placenta and lung.2 Publications

Gene expression databases

BgeeiQ86XI6.
CleanExiHS_PPP1R3B.
GenevisibleiQ86XI6. HS.

Organism-specific databases

HPAiHPA028731.

Interactioni

Subunit structurei

Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi122786. 9 interactions.
IntActiQ86XI6. 6 interactions.
MINTiMINT-1180809.
STRINGi9606.ENSP00000308318.

Structurei

Secondary structure

1
285
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi110 – 1145Combined sources
Helixi121 – 13010Combined sources
Beta strandi131 – 14111Combined sources
Beta strandi144 – 1518Combined sources
Beta strandi154 – 1563Combined sources
Beta strandi159 – 1679Combined sources
Beta strandi170 – 1767Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi187 – 1893Combined sources
Beta strandi191 – 1944Combined sources
Beta strandi209 – 2168Combined sources
Beta strandi219 – 2246Combined sources
Helixi225 – 2273Combined sources
Beta strandi232 – 2343Combined sources
Turni235 – 2373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EEFNMR-A105-253[»]
ProteinModelPortaliQ86XI6.
SMRiQ86XI6. Positions 110-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86XI6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 233109CBM21PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi62 – 654PP1-binding motif

Domaini

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL.By similarity

Sequence similaritiesi

Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ86XI6.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG7H4DTW.
PhylomeDBiQ86XI6.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86XI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAVDIEYRY NCMAPSLRQE RFAFKISPKP SKPLRPCIQL SSKNEASGMV
60 70 80 90 100
APAVQEKKVK KRVSFADNQG LALTMVKVFS EFDDPLDMPF NITELLDNIV
110 120 130 140 150
SLTTAESESF VLDFSQPSAD YLDFRNRLQA DHVCLENCVL KDKAIAGTVK
160 170 180 190 200
VQNLAFEKTV KIRMTFDTWK SYTDFPCQYV KDTYAGSDRD TFSFDISLPE
210 220 230 240 250
KIQSYERMEF AVYYECNGQT YWDSNRGKNY RIIRAELKST QGMTKPHSGP
260 270 280
DLGISFDQFG SPRCSYGLFP EWPSYLGYEK LGPYY
Length:285
Mass (Da):32,695
Last modified:June 1, 2003 - v1
Checksum:i77178FE100947989
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491V → A in BAB14811 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 481G → E.
Corresponds to variant rs3748140 [ dbSNP | Ensembl ].
VAR_039814

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.
CCDSiCCDS5973.1.
RefSeqiNP_001188258.1. NM_001201329.1.
NP_078883.2. NM_024607.3.
XP_006716316.1. XM_006716253.2.
XP_011542138.1. XM_011543836.1.
UniGeneiHs.458513.

Genome annotation databases

EnsembliENST00000310455; ENSP00000308318; ENSG00000173281.
ENST00000519699; ENSP00000428642; ENSG00000173281.
GeneIDi79660.
KEGGihsa:79660.
UCSCiuc003wsn.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.
CCDSiCCDS5973.1.
RefSeqiNP_001188258.1. NM_001201329.1.
NP_078883.2. NM_024607.3.
XP_006716316.1. XM_006716253.2.
XP_011542138.1. XM_011543836.1.
UniGeneiHs.458513.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EEFNMR-A105-253[»]
ProteinModelPortaliQ86XI6.
SMRiQ86XI6. Positions 110-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122786. 9 interactions.
IntActiQ86XI6. 6 interactions.
MINTiMINT-1180809.
STRINGi9606.ENSP00000308318.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

PTM databases

iPTMnetiQ86XI6.
PhosphoSiteiQ86XI6.

Polymorphism and mutation databases

BioMutaiPPP1R3B.
DMDMi74727837.

Proteomic databases

MaxQBiQ86XI6.
PaxDbiQ86XI6.
PRIDEiQ86XI6.

Protocols and materials databases

DNASUi79660.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310455; ENSP00000308318; ENSG00000173281.
ENST00000519699; ENSP00000428642; ENSG00000173281.
GeneIDi79660.
KEGGihsa:79660.
UCSCiuc003wsn.5. human.

Organism-specific databases

CTDi79660.
GeneCardsiPPP1R3B.
H-InvDBHIX0007298.
HGNCiHGNC:14942. PPP1R3B.
HPAiHPA028731.
MIMi610541. gene.
neXtProtiNX_Q86XI6.
PharmGKBiPA33652.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ86XI6.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG7H4DTW.
PhylomeDBiQ86XI6.
TreeFamiTF105537.

Miscellaneous databases

ChiTaRSiPPP1R3B. human.
EvolutionaryTraceiQ86XI6.
GenomeRNAii79660.
PROiQ86XI6.
SOURCEiSearch...

Gene expression databases

BgeeiQ86XI6.
CleanExiHS_PPP1R3B.
GenevisibleiQ86XI6. HS.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver and Retinoblastoma.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  4. "Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver."
    Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W.
    Diabetes 51:591-598(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle."
    Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M.
    Biochem. J. 405:107-113(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Solution structure of the CBM21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2007) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 105-253.

Entry informationi

Entry nameiPPR3B_HUMAN
AccessioniPrimary (citable) accession number: Q86XI6
Secondary accession number(s): B3KTV3, Q9H812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.