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Protein

Protein phosphatase 1 regulatory subunit 3B

Gene

PPP1R3B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciZFISH:HS16195-MONOMER.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name:
PP1 subunit R4
Protein phosphatase 1 subunit GL
Short name:
PTG
Gene namesi
Name:PPP1R3B
Synonyms:PPP1R4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:14942. PPP1R3B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi79660.
OpenTargetsiENSG00000173281.
PharmGKBiPA33652.

Polymorphism and mutation databases

BioMutaiPPP1R3B.
DMDMi74727837.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245431 – 285Protein phosphatase 1 regulatory subunit 3BAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei261PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86XI6.
MaxQBiQ86XI6.
PaxDbiQ86XI6.
PeptideAtlasiQ86XI6.
PRIDEiQ86XI6.

PTM databases

iPTMnetiQ86XI6.
PhosphoSitePlusiQ86XI6.

Expressioni

Tissue specificityi

Highly expressed in the liver and, at lower levels, in skeletal muscle, including in vastus lateralis, gastrocnemius and soleus (at protein level). Highest mRNA levels are observed in skeletal muscle, and only moderate levels in liver and heart. Weak expression in placenta and lung.2 Publications

Gene expression databases

BgeeiENSG00000173281.
CleanExiHS_PPP1R3B.
GenevisibleiQ86XI6. HS.

Organism-specific databases

HPAiHPA028731.

Interactioni

Subunit structurei

Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi122786. 9 interactors.
IntActiQ86XI6. 6 interactors.
MINTiMINT-1180809.
STRINGi9606.ENSP00000308318.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi110 – 114Combined sources5
Helixi121 – 130Combined sources10
Beta strandi131 – 141Combined sources11
Beta strandi144 – 151Combined sources8
Beta strandi154 – 156Combined sources3
Beta strandi159 – 167Combined sources9
Beta strandi170 – 176Combined sources7
Beta strandi183 – 185Combined sources3
Beta strandi187 – 189Combined sources3
Beta strandi191 – 194Combined sources4
Beta strandi209 – 216Combined sources8
Beta strandi219 – 224Combined sources6
Helixi225 – 227Combined sources3
Beta strandi232 – 234Combined sources3
Turni235 – 237Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EEFNMR-A105-253[»]
ProteinModelPortaliQ86XI6.
SMRiQ86XI6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86XI6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini125 – 233CBM21PROSITE-ProRule annotationAdd BLAST109

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi62 – 65PP1-binding motif4

Domaini

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL.By similarity

Sequence similaritiesi

Contains 1 CBM21 (carbohydrate binding type-21) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ86XI6.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG091G0GB5.
PhylomeDBiQ86XI6.
TreeFamiTF105537.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86XI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAVDIEYRY NCMAPSLRQE RFAFKISPKP SKPLRPCIQL SSKNEASGMV
60 70 80 90 100
APAVQEKKVK KRVSFADNQG LALTMVKVFS EFDDPLDMPF NITELLDNIV
110 120 130 140 150
SLTTAESESF VLDFSQPSAD YLDFRNRLQA DHVCLENCVL KDKAIAGTVK
160 170 180 190 200
VQNLAFEKTV KIRMTFDTWK SYTDFPCQYV KDTYAGSDRD TFSFDISLPE
210 220 230 240 250
KIQSYERMEF AVYYECNGQT YWDSNRGKNY RIIRAELKST QGMTKPHSGP
260 270 280
DLGISFDQFG SPRCSYGLFP EWPSYLGYEK LGPYY
Length:285
Mass (Da):32,695
Last modified:June 1, 2003 - v1
Checksum:i77178FE100947989
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149V → A in BAB14811 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03981448G → E.Corresponds to variant rs3748140dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.
CCDSiCCDS5973.1.
RefSeqiNP_001188258.1. NM_001201329.1.
NP_078883.2. NM_024607.3.
XP_006716316.1. XM_006716253.3.
XP_016869324.1. XM_017013835.1.
UniGeneiHs.458513.

Genome annotation databases

EnsembliENST00000310455; ENSP00000308318; ENSG00000173281.
ENST00000519699; ENSP00000428642; ENSG00000173281.
GeneIDi79660.
KEGGihsa:79660.
UCSCiuc003wsn.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.
CCDSiCCDS5973.1.
RefSeqiNP_001188258.1. NM_001201329.1.
NP_078883.2. NM_024607.3.
XP_006716316.1. XM_006716253.3.
XP_016869324.1. XM_017013835.1.
UniGeneiHs.458513.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EEFNMR-A105-253[»]
ProteinModelPortaliQ86XI6.
SMRiQ86XI6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122786. 9 interactors.
IntActiQ86XI6. 6 interactors.
MINTiMINT-1180809.
STRINGi9606.ENSP00000308318.

Protein family/group databases

CAZyiCBM21. Carbohydrate-Binding Module Family 21.

PTM databases

iPTMnetiQ86XI6.
PhosphoSitePlusiQ86XI6.

Polymorphism and mutation databases

BioMutaiPPP1R3B.
DMDMi74727837.

Proteomic databases

EPDiQ86XI6.
MaxQBiQ86XI6.
PaxDbiQ86XI6.
PeptideAtlasiQ86XI6.
PRIDEiQ86XI6.

Protocols and materials databases

DNASUi79660.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310455; ENSP00000308318; ENSG00000173281.
ENST00000519699; ENSP00000428642; ENSG00000173281.
GeneIDi79660.
KEGGihsa:79660.
UCSCiuc003wsn.5. human.

Organism-specific databases

CTDi79660.
DisGeNETi79660.
GeneCardsiPPP1R3B.
H-InvDBHIX0007298.
HGNCiHGNC:14942. PPP1R3B.
HPAiHPA028731.
MIMi610541. gene.
neXtProtiNX_Q86XI6.
OpenTargetsiENSG00000173281.
PharmGKBiPA33652.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3986. Eukaryota.
ENOG4111FT1. LUCA.
GeneTreeiENSGT00530000062978.
HOGENOMiHOG000026799.
HOVERGENiHBG052744.
InParanoidiQ86XI6.
KOiK07189.
OMAiMVKVFSE.
OrthoDBiEOG091G0GB5.
PhylomeDBiQ86XI6.
TreeFamiTF105537.

Enzyme and pathway databases

BioCyciZFISH:HS16195-MONOMER.

Miscellaneous databases

ChiTaRSiPPP1R3B. human.
EvolutionaryTraceiQ86XI6.
GenomeRNAii79660.
PROiQ86XI6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000173281.
CleanExiHS_PPP1R3B.
GenevisibleiQ86XI6. HS.

Family and domain databases

InterProiIPR005036. CBM21_dom.
IPR017434. Pase-1_reg-su_3B/C/D_met.
[Graphical view]
PfamiPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFiPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEiPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPR3B_HUMAN
AccessioniPrimary (citable) accession number: Q86XI6
Secondary accession number(s): B3KTV3, Q9H812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.