Q86XI6 (PPR3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 76.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein phosphatase 1 regulatory subunit 3B Alternative name(s): Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL Protein phosphatase 1 regulatory subunit 4 Short name=PP1 subunit R4 Protein phosphatase 1 subunit GL Short name=PTG | ||||
| Gene names |
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| Organism | Homo sapiens (Human) [Reference proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo![]() |
Protein attributes
| Sequence length | 285 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes By similarity. |
| Subunit structure | Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity By similarity. |
| Tissue specificity | Highly expressed in the liver and, at lower levels, in skeletal muscle, including in vastus lateralis, gastrocnemius and soleus (at protein level). Highest mRNA levels are observed in skeletal muscle, and only moderate levels in liver and heart. Weak expression in placenta and lung. Ref.4 Ref.5 |
| Domain | The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL By similarity. |
| Sequence similarities | Contains 1 CBM21 (carbohydrate binding type-21) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Glycogen metabolism |
| Coding sequence diversity | Polymorphism |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of glycogen biosynthetic processInferred from electronic annotation. Source: Compara regulation of glycogen catabolic processInferred from electronic annotation. Source: Compara |
| Cellular_component | glycogen granule Inferred from electronic annotation. Source: Compara intracellular membrane-bounded organelleInferred from electronic annotation. Source: Compara protein phosphatase type 1 complexInferred from electronic annotation. Source: Compara |
| Molecular_function | protein phosphatase regulator activity Inferred from electronic annotation. Source: Compara |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 285 | 285 | Protein phosphatase 1 regulatory subunit 3B | PRO_0000324543 | ||||||||||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||||||||||
| Domain | 125 – 233 | 109 | CBM21 | |||||||||||||||||||||||||||||||||
| Motif | 62 – 65 | 4 | PP1-binding motif | |||||||||||||||||||||||||||||||||
Natural variations | ||||||||||||||||||||||||||||||||||||
| Natural variant | 48 | 1 | G → E. Corresponds to variant rs3748140 [ dbSNP | Ensembl ]. | VAR_039814 | ||||||||||||||||||||||||||||||||
Experimental info | ||||||||||||||||||||||||||||||||||||
| Sequence conflict | 149 | 1 | V → A in BAB14811. Ref.1 | |||||||||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||||||||||
| Beta strand | 110 – 114 | 5 | ||||||||||||||||||||||||||||||||||
| Helix | 121 – 130 | 10 | ||||||||||||||||||||||||||||||||||
| Beta strand | 131 – 141 | 11 | ||||||||||||||||||||||||||||||||||
| Beta strand | 144 – 151 | 8 | ||||||||||||||||||||||||||||||||||
| Beta strand | 154 – 156 | 3 | ||||||||||||||||||||||||||||||||||
| Beta strand | 159 – 167 | 9 | ||||||||||||||||||||||||||||||||||
| Beta strand | 170 – 176 | 7 | ||||||||||||||||||||||||||||||||||
| Beta strand | 183 – 185 | 3 | ||||||||||||||||||||||||||||||||||
| Beta strand | 187 – 189 | 3 | ||||||||||||||||||||||||||||||||||
| Beta strand | 191 – 194 | 4 | ||||||||||||||||||||||||||||||||||
| Beta strand | 209 – 216 | 8 | ||||||||||||||||||||||||||||||||||
| Beta strand | 219 – 224 | 6 | ||||||||||||||||||||||||||||||||||
| Helix | 225 – 227 | 3 | ||||||||||||||||||||||||||||||||||
| Beta strand | 232 – 234 | 3 | ||||||||||||||||||||||||||||||||||
| Turn | 235 – 237 | 3 | ||||||||||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Retinoblastoma. |
| [2] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. |
| [4] | "Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [5] | "Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle." Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M. Biochem. J. 405:107-113(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [6] | "Solution structure of the CBM21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 105-253. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AK024067 mRNA. Translation: BAB14811.1. AK096119 mRNA. Translation: BAG53215.1. CH471157 Genomic DNA. Translation: EAW65572.1. BC043388 mRNA. Translation: AAH43388.1. | ||||||||||||
| IPI | IPI00002415. | ||||||||||||
| RefSeq | NP_001188258.1. NM_001201329.1. NP_078883.2. NM_024607.3. | ||||||||||||
| UniGene | Hs.458513. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | Q86XI6. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| IntAct | Q86XI6. 4 interactions. | ||||||||||||
| MINT | MINT-1180809. | ||||||||||||
| STRING | 9606.ENSP00000308318. | ||||||||||||
Protein family/group databases | |||||||||||||
| CAZy | CBM21. Carbohydrate-Binding Module Family 21. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | Q86XI6. | ||||||||||||
Polymorphism databases | |||||||||||||
| DMDM | 74727837. | ||||||||||||
Proteomic databases | |||||||||||||
| PaxDb | Q86XI6. | ||||||||||||
| PRIDE | Q86XI6. | ||||||||||||
Protocols and materials databases | |||||||||||||
| DNASU | 79660. | ||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENST00000310455; ENSP00000308318; ENSG00000173281. ENST00000519699; ENSP00000428642; ENSG00000173281. | ||||||||||||
| GeneID | 79660. | ||||||||||||
| KEGG | hsa:79660. | ||||||||||||
| UCSC | uc003wsn.4. human. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 79660. | ||||||||||||
| GeneCards | GC08M009032. | ||||||||||||
| H-InvDB | HIX0007298. | ||||||||||||
| HGNC | HGNC:14942. PPP1R3B. | ||||||||||||
| HPA | HPA028731. | ||||||||||||
| MIM | 610541. gene. | ||||||||||||
| neXtProt | NX_Q86XI6. | ||||||||||||
| PharmGKB | PA33652. | ||||||||||||
| GenAtlas | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | NOG328473. | ||||||||||||
| HOGENOM | HOG000026799. | ||||||||||||
| HOVERGEN | HBG052744. | ||||||||||||
| InParanoid | Q86XI6. | ||||||||||||
| KO | K07189. | ||||||||||||
| OMA | LRPCIQL. | ||||||||||||
| OrthoDB | EOG47PX72. | ||||||||||||
| PhylomeDB | Q86XI6. | ||||||||||||
Gene expression databases | |||||||||||||
| Bgee | Q86XI6. | ||||||||||||
| CleanEx | HS_PPP1R3B. | ||||||||||||
| Genevestigator | Q86XI6. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR005036. CBM_21. IPR017434. Pase-1_Glycogen_target-su_met. [Graphical view] | ||||||||||||
| Pfam | PF03370. CBM_21. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF038207. PP1_GT_animal. 1 hit. | ||||||||||||
| PROSITE | PS51159. CBM21. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| ChiTaRS | PPP1R3B. human. | ||||||||||||
| EvolutionaryTrace | Q86XI6. | ||||||||||||
| GenomeRNAi | 79660. | ||||||||||||
| NextBio | 68848. | ||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | PPR3B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q86XI6 Secondary accession number(s): B3KTV3, Q9H812 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 8 Human chromosome 8: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with
