Reviewed, UniProtKB/Swiss-Prot Q86XI6 (PPR3B_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
    Protein phosphatase 1 regulatory subunit 4
      Short name=R4
    Protein phosphatase 1 subunit GL
    Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL

Gene names

Name:	PPP1R3B
Synonyms:	PPP1R4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes By similarity.
Subunit structure	Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity By similarity.
Tissue specificity	Highly expressed in liver. Expressed moderately in skeletal muscle and myotubes (at protein level). Expressed predominantly in heart and skeletal muscle. Expressed moderately in liver. Expressed weakly in placenta, lung and kidney. Ref.4 Ref.5
Domain	The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL By similarity.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Coding sequence diversity	Polymorphism
Technical term	3D-structure
Gene Ontology (GO)
Biological process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Protein phosphatase 1 regulatory subunit 3B		PRO_0000324543
Regions
	Domain	125 – 233	109	CBM21
Natural variations
	Natural variant	48	1	G → E: dbSNP rs3748140.		VAR_039814
Experimental info
	Sequence conflict	149	1	V → A in BAB14811. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86XI6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 77178FE100947989	FASTA	285	32,695
	10 20 30 40 50 60 MMAVDIEYRY NCMAPSLRQE RFAFKISPKP SKPLRPCIQL SSKNEASGMV APAVQEKKVK 70 80 90 100 110 120 KRVSFADNQG LALTMVKVFS EFDDPLDMPF NITELLDNIV SLTTAESESF VLDFSQPSAD 130 140 150 160 170 180 YLDFRNRLQA DHVCLENCVL KDKAIAGTVK VQNLAFEKTV KIRMTFDTWK SYTDFPCQYV 190 200 210 220 230 240 KDTYAGSDRD TFSFDISLPE KIQSYERMEF AVYYECNGQT YWDSNRGKNY RIIRAELKST 250 260 270 280 QGMTKPHSGP DLGISFDQFG SPRCSYGLFP EWPSYLGYEK LGPYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Retinoblastoma.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed: 11872655] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle." Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M. Biochem. J. 405:107-113(2007) [PubMed: 17555403] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Solution structure of the CBM21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 105-253.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.

IPI

IPI00002415.

RefSeq

NP_078883.2.

UniGene

Hs.458513

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EEF	NMR	-	A	105-253	[»]

ModBase

Search...

Protein family/group databases

CAZy

CBM21. Carbohydrate-Binding Module Family 21.

PTM databases

PhosphoSite

Q86XI6.

Proteomic databases

PRIDE

Q86XI6.

Genome annotation databases

Ensembl

ENSG00000173281. Homo sapiens. [Contig view]

GeneID

79660.

KEGG

hsa:79660.

Organism-specific databases

GeneCards

GC08M009032.

HGNC

HGNC:14942. PPP1R3B.

MIM

610541. gene.

PharmGKB

PA33652.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q86XI6.

HOVERGEN

Q86XI6.

OMA

Q86XI6. PLRPCIQ.

Gene expression databases

ArrayExpress

Q86XI6.

Bgee

Q86XI6.

CleanEx

HS_PPP1R3B.

Family and domain databases

InterPro

IPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view]

Pfam

PF03370. CBM_21. 1 hit.
[Graphical view]

PIRSF

PIRSF038207. PP1_GT_animal. 1 hit.

PROSITE

PS51159. CBM21. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

68848.

SOURCE

Search...

Entry information

Entry name

PPR3B_HUMAN

Accession

Primary (citable) accession number: Q86XI6
Secondary accession number(s): B3KTV3, Q9H812

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2003
Last modified:	May 5, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)