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Reviewed, UniProtKB/Swiss-Prot Q86XI6 (PPR3B_HUMAN)

Last modified May 5, 2009. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein phosphatase 1 regulatory subunit 3B
Alternative name(s):
    Protein phosphatase 1 regulatory subunit 4
      Short name=R4
    Protein phosphatase 1 subunit GL
    Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Gene names
Name: PPP1R3B
Synonyms: PPP1R4
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes By similarity.

Subunit structure

Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity By similarity.

Tissue specificity

Highly expressed in liver. Expressed moderately in skeletal muscle and myotubes (at protein level). Expressed predominantly in heart and skeletal muscle. Expressed moderately in liver. Expressed weakly in placenta, lung and kidney. Ref.4 Ref.5

Domain

The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL By similarity.

Sequence similarities

Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glycogen metabolism
   Coding sequence diversityPolymorphism
   Technical term3D-structure
Gene Ontology (GO)
   Biological processglycogen metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Protein phosphatase 1 regulatory subunit 3B
PRO_0000324543

Regions

Domain125 – 233109CBM21

Natural variations

Natural variant481G → E: dbSNP rs3748140.
VAR_039814

Experimental info

Sequence conflict1491V → A in BAB14811. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q86XI6-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 77178FE100947989

FASTA28532,695
        10         20         30         40         50         60 
MMAVDIEYRY NCMAPSLRQE RFAFKISPKP SKPLRPCIQL SSKNEASGMV APAVQEKKVK 

        70         80         90        100        110        120 
KRVSFADNQG LALTMVKVFS EFDDPLDMPF NITELLDNIV SLTTAESESF VLDFSQPSAD 

       130        140        150        160        170        180 
YLDFRNRLQA DHVCLENCVL KDKAIAGTVK VQNLAFEKTV KIRMTFDTWK SYTDFPCQYV 

       190        200        210        220        230        240 
KDTYAGSDRD TFSFDISLPE KIQSYERMEF AVYYECNGQT YWDSNRGKNY RIIRAELKST 

       250        260        270        280 
QGMTKPHSGP DLGISFDQFG SPRCSYGLFP EWPSYLGYEK LGPYY 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver and Retinoblastoma.
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[4]"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver."
Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W.
Diabetes 51:591-598(2002) [PubMed: 11872655] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle."
Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M.
Biochem. J. 405:107-113(2007) [PubMed: 17555403] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Solution structure of the CBM21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B."
RIKEN structural genomics initiative (RSGI)
Submitted (AUG-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 105-253.

Cross-references

Sequence databases

AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.
IPIIPI00002415.
RefSeqNP_078883.2.
UniGeneHs.458513

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2EEFNMR-A105-253[»]
ModBaseSearch...

Protein family/group databases

CAZyCBM21. Carbohydrate-Binding Module Family 21.

PTM databases

PhosphoSiteQ86XI6.

Proteomic databases

PRIDEQ86XI6.

Genome annotation databases

EnsemblENSG00000173281. Homo sapiens. [Contig view]
GeneID79660.
KEGGhsa:79660.

Organism-specific databases

GeneCardsGC08M009032.
HGNCHGNC:14942. PPP1R3B.
MIM610541. gene.
PharmGKBPA33652.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ86XI6.
HOVERGENQ86XI6.
OMAQ86XI6. PLRPCIQ.

Gene expression databases

ArrayExpressQ86XI6.
BgeeQ86XI6.
CleanExHS_PPP1R3B.

Family and domain databases

InterProIPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view]
PfamPF03370. CBM_21. 1 hit.
[Graphical view]
PIRSFPIRSF038207. PP1_GT_animal. 1 hit.
PROSITEPS51159. CBM21. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio68848.
SOURCESearch...

Entry information

Entry namePPR3B_HUMAN
AccessionPrimary (citable) accession number: Q86XI6
Secondary accession number(s): B3KTV3, Q9H812
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2003
Last modified: May 5, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents