Q86XI6 (PPR3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1 regulatory subunit 3B

Alternative name(s):
Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL
Protein phosphatase 1 regulatory subunit 4
Short name=PP1 subunit R4
Protein phosphatase 1 subunit GL
Short name=PTG

Gene names

Name:	PPP1R3B
Synonyms:	PPP1R4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes By similarity.
Subunit structure	Interacts with glycogen, PPP1CC catalytic subunit of PP1 and PYGL. Associates with glycogen particles. Forms complexes with debranching enzyme, glycogen phosphorylase, glycogen synthase and phosphorylase kinase which is necessary for its regulation of PP1 activity By similarity.
Tissue specificity	Highly expressed in the liver and, at lower levels, in skeletal muscle, including in vastus lateralis, gastrocnemius and soleus (at protein level). Highest mRNA levels are observed in skeletal muscle, and only moderate levels in liver and heart. Weak expression in placenta and lung. Ref.4 Ref.5
Domain	The N-terminal region is required for binding to PP1, the central region is required for binding to glycogen and the C-terminal region is required for binding to PYGL By similarity.
Sequence similarities	Contains 1 CBM21 (carbohydrate binding type-21) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Coding sequence diversity	Polymorphism
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of glycogen biosynthetic process Inferred from electronic annotation. Source: Compara regulation of glycogen catabolic process Inferred from electronic annotation. Source: Compara
Cellular_component	glycogen granule Inferred from electronic annotation. Source: Compara intracellular membrane-bounded organelle Inferred from electronic annotation. Source: Compara protein phosphatase type 1 complex Inferred from electronic annotation. Source: Compara
Molecular_function	protein phosphatase regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Protein phosphatase 1 regulatory subunit 3B

PRO_0000324543

Regions

Domain

125 – 233

109

CBM21

Motif

62 – 65

4

PP1-binding motif

Natural variations

Natural variant

48

1

G → E.
Corresponds to variant rs3748140 [ dbSNP | Ensembl ].

VAR_039814

Experimental info

Sequence conflict

149

1

V → A in BAB14811. Ref.1

Secondary structure

1

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285

Helix Strand Turn

Details...

Beta strand

110 – 114

5

Helix

121 – 130

10

Beta strand

131 – 141

11

Beta strand

144 – 151

8

Beta strand

154 – 156

3

Beta strand

159 – 167

9

Beta strand

170 – 176

7

Beta strand

183 – 185

3

Beta strand

187 – 189

3

Beta strand

191 – 194

4

Beta strand

209 – 216

8

Beta strand

219 – 224

6

Helix

225 – 227

3

Beta strand

232 – 234

3

Turn

235 – 237

3

Sequences

	Sequence		Length	Mass (Da)
	Q86XI6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 77178FE100947989	FASTA	285	32,695
	10 20 30 40 50 60 MMAVDIEYRY NCMAPSLRQE RFAFKISPKP SKPLRPCIQL SSKNEASGMV APAVQEKKVK 70 80 90 100 110 120 KRVSFADNQG LALTMVKVFS EFDDPLDMPF NITELLDNIV SLTTAESESF VLDFSQPSAD 130 140 150 160 170 180 YLDFRNRLQA DHVCLENCVL KDKAIAGTVK VQNLAFEKTV KIRMTFDTWK SYTDFPCQYV 190 200 210 220 230 240 KDTYAGSDRD TFSFDISLPE KIQSYERMEF AVYYECNGQT YWDSNRGKNY RIIRAELKST 250 260 270 280 QGMTKPHSGP DLGISFDQFG SPRCSYGLFP EWPSYLGYEK LGPYY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Retinoblastoma.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Human skeletal muscle expresses a glycogen-targeting subunit of PP1 that is identical to the insulin-sensitive glycogen-targeting subunit G(L) of liver." Munro S., Cuthbertson D.J., Cunningham J., Sales M., Cohen P.T.W. Diabetes 51:591-598(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Expression and glycogenic effect of glycogen-targeting protein phosphatase 1 regulatory subunit GL in cultured human muscle." Montori-Grau M., Guitart M., Lerin C., Andreu A.L., Newgard C.B., Garcia-Martinez C., Gomez-Foix A.M. Biochem. J. 405:107-113(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Solution structure of the CBM21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B." RIKEN structural genomics initiative (RSGI) Submitted (AUG-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 105-253.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK024067 mRNA. Translation: BAB14811.1.
AK096119 mRNA. Translation: BAG53215.1.
CH471157 Genomic DNA. Translation: EAW65572.1.
BC043388 mRNA. Translation: AAH43388.1.

IPI

IPI00002415.

RefSeq

NP_001188258.1. NM_001201329.1.
NP_078883.2. NM_024607.3.

UniGene

Hs.458513.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EEF	NMR	-	A	105-253	[»]

ProteinModelPortal

Q86XI6.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q86XI6. 4 interactions.

MINT

MINT-1180809.

STRING

9606.ENSP00000308318.

Protein family/group databases

CAZy

CBM21. Carbohydrate-Binding Module Family 21.

PTM databases

PhosphoSite

Q86XI6.

Polymorphism databases

DMDM

74727837.

Proteomic databases

PaxDb

Q86XI6.

PRIDE

Q86XI6.

Protocols and materials databases

DNASU

79660.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000310455; ENSP00000308318; ENSG00000173281.
ENST00000519699; ENSP00000428642; ENSG00000173281.

GeneID

79660.

KEGG

hsa:79660.

UCSC

uc003wsn.4. human.

Organism-specific databases

CTD

79660.

GeneCards

GC08M009032.

H-InvDB

HIX0007298.

HGNC

HGNC:14942. PPP1R3B.

HPA

HPA028731.

MIM

610541. gene.

neXtProt

NX_Q86XI6.

PharmGKB

PA33652.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG328473.

HOGENOM

HOG000026799.

HOVERGEN

HBG052744.

InParanoid

Q86XI6.

KO

K07189.

OMA

LRPCIQL.

OrthoDB

EOG47PX72.

PhylomeDB

Q86XI6.

Gene expression databases

Bgee

Q86XI6.

CleanEx

HS_PPP1R3B.

Genevestigator

Q86XI6.

Family and domain databases

InterPro

IPR005036. CBM_21.
IPR017434. Pase-1_Glycogen_target-su_met.
[Graphical view]

Pfam

PF03370. CBM_21. 1 hit.
[Graphical view]

PIRSF

PIRSF038207. PP1_GT_animal. 1 hit.

PROSITE

PS51159. CBM21. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PPP1R3B. human.

EvolutionaryTrace

Q86XI6.

GenomeRNAi

79660.

NextBio

68848.

SOURCE

Search...

Entry information

Entry name

PPR3B_HUMAN

Accession

Primary (citable) accession number: Q86XI6
Secondary accession number(s): B3KTV3, Q9H812

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.