##gff-version 3
Q86XE5	UniProtKB	Transit peptide	1	25	.	.	.	Note=Mitochondrion;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q0P5I5	
Q86XE5	UniProtKB	Chain	26	327	.	.	.	ID=PRO_0000273346;Note=4-hydroxy-2-oxoglutarate aldolase%2C mitochondrial	
Q86XE5	UniProtKB	Active site	196	196	.	.	.	Note=Schiff-base intermediate with substrate;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Binding site	77	78	.	.	.	.	
Q86XE5	UniProtKB	Binding site	198	198	.	.	.	.	
Q86XE5	UniProtKB	Binding site	222	222	.	.	.	.	
Q86XE5	UniProtKB	Site	168	168	.	.	.	Note=Involved in proton transfer during cleavage	
Q86XE5	UniProtKB	Alternative sequence	71	233	.	.	.	ID=VSP_022515;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|PubMed:15489334;Dbxref=PMID:15489334	
Q86XE5	UniProtKB	Natural variant	257	257	.	.	.	ID=VAR_064035;Note=In HP3. C->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20797690;Dbxref=dbSNP:rs267606764,PMID:20797690	
Q86XE5	UniProtKB	Natural variant	287	287	.	.	.	ID=VAR_064036;Note=In HP3. G->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20797690;Dbxref=dbSNP:rs138207257,PMID:20797690	
Q86XE5	UniProtKB	Natural variant	315	315	.	.	.	ID=VAR_064037;Note=In HP3. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20797690;Dbxref=PMID:20797690	
Q86XE5	UniProtKB	Mutagenesis	77	77	.	.	.	Note=2-fold decrease in kcat and a nearly 8-fold increase in KM. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	77	77	.	.	.	Note=Significant loss of activity. S->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	78	78	.	.	.	Note=6-fold increase in KM. N->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	78	78	.	.	.	Note=25-fold increase in KM. N->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	140	140	.	.	.	Note=No change in activity. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	168	168	.	.	.	Note=No enzymatic activity. Y->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	196	196	.	.	.	Note=No enzymatic activity. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	198	198	.	.	.	Note=2.5-fold decrease in kcat and 4.2 fold increase in KM. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Mutagenesis	198	198	.	.	.	Note=7-fold increase in KM. S->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21998747;Dbxref=PMID:21998747	
Q86XE5	UniProtKB	Beta strand	37	39	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	54	64	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	70	76	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	77	79	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	81	83	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	86	98	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	104	109	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	115	127	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	131	136	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	142	144	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	147	160	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	165	169	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	171	174	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	180	187	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	192	197	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	202	211	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Turn	212	214	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	218	223	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	224	226	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	227	233	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Beta strand	237	239	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	241	244	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	247	258	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	262	278	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Turn	279	283	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	284	295	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Helix	311	322	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O	
Q86XE5	UniProtKB	Turn	323	325	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3S5O