Q86X95 (CIR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Corepressor interacting with RBPJ 1

Alternative name(s):
CBF1-interacting corepressor
Recepin

Gene names

Name:	CIR1
Synonyms:	CIR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May modulate splice site selection during alternative splicing of pre-mRNAs By similarity. Regulates transcription and acts as corepressor for RBPJ. Recruits RBPJ to the Sin3-histone deacetylase complex (HDAC). Required for RBPJ-mediated repression of transcription. Ref.1 Ref.9
Subunit structure	Interacts with RP9, SNW1, SFRS1, SFRS2 and U2AF1 By similarity. Interacts with Epstein-Barr virus RPMS1. Component of the histone deacetylase complex. Component of the Notch corepressor complex. Interacts with RBPJ, SAP30 and HDAC2 and NKAP. Interacts with NEK6. Ref.1 Ref.6 Ref.9 Ref.11
Subcellular location	Nucleus speckle. Cytoplasm › cytoskeleton › centrosome. Note: Co-localizes with NEK6 in the centrosome. Ref.1 Ref.6 Ref.11
Tissue specificity	Highly expressed in heart, brain, placenta, liver, skeletal muscle and pancreas. Ref.1
Post-translational modification	Phosphorylated by NEK6. Ref.7 Ref.8 Ref.10 Ref.11
Sequence caution	The sequence AAA17853.1 differs from that shown. Reason: Frameshift at positions 135, 151, 177, 203, 261, 299, 391 and 410. The sequence AAH21175.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence. The sequence AAH38987.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Cytoplasm Cytoskeleton Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.1. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction Ref.9Ref.1. Source: UniProtKB sequence-specific DNA binding transcription factor activity Traceable author statement. Source: ProtInc transcription corepressor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SRSF1	Q07955	3	EBI-627102,EBI-398920
SRSF2	Q01130	4	EBI-627102,EBI-627047
U2AF1	Q01081	4	EBI-627102,EBI-632461

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86X95-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86X95-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-202: GPSMHPSELI...NDPSQEYVAS → DAVLQIEGFW...KCTGEKLDRK
     203-450: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Corepressor interacting with RBPJ 1		PRO_0000247984
Regions
	Region	1 – 121	121	Interaction with RBPJ
	Region	204 – 232	29	Interaction with RP9 By similarity
	Motif	235 – 245	11	Nuclear localization signal Potential
	Motif	291 – 298	8	Nuclear localization signal Potential
	Compositional bias	214 – 368	155	Lys/Ser-rich
	Compositional bias	369 – 450	82	Arg/Ser-rich (RS domain)
Amino acid modifications
	Modified residue	202	1	Phosphoserine Ref.7 Ref.8 Ref.10
Natural variations
	Alternative sequence	162 – 202	41	GPSMH…EYVAS → DAVLQIEGFWAIDAPLGANS GDEKQWVCTETKCTGEKLDR K in isoform 2.		VSP_020091
	Alternative sequence	203 – 450	248	Missing in isoform 2.		VSP_020092
Experimental info
	Sequence conflict	48	1	K → R in AAD05243. Ref.1
	Sequence conflict	113	1	N → S in AAD05243. Ref.1
	Sequence conflict	172	1	A → G in AAA17853. Ref.2
	Sequence conflict	325	1	S → T in AAD05243. Ref.1
	Sequence conflict	325	1	S → T in AAA17853. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5D0C1CBD9C87569D	FASTA	450	52,313
	10 20 30 40 50 60 MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG 70 80 90 100 110 120 DERVKNGLNF MYEAPPGAKK ENKEKEETEG ETEYKFEWQK GAPREKYAKD DMNIRDQPFG 130 140 150 160 170 180 IQVRNVRCIK CHKWGHVNTD RECPLFGLSG INASSVPTDG SGPSMHPSEL IAEMRNSGFA 190 200 210 220 230 240 LKRNVLGRNL TANDPSQEYV ASEGEEDPEV EFLKSLTTKQ KQKLLRKLDR LEKKKKKKDR 250 260 270 280 290 300 KKKKFQKSRS KHKKHKSSSS SSSSSSSSSS TETSESSSES ESNNKEKKIQ RKKRKKNKCS 310 320 330 340 350 360 GHNNSDSEEK DKSKKRKLHE ELSSSHHNRE KAKEKPRFLK HESSREDSKW SHSDSDKKSR 370 380 390 400 410 420 THKHSPEKRG SERKEGSSRS HGREERSRRS RSRSPGSYKQ RETRKRAQRN PGEEQSRRND 430 440 450 SRSHGTDLYR GEKMYREHPG GTHTKVTQRE
	Isoform 2 [UniParc]. Checksum: 6C16582B3E91B8DE	FASTA	202	23,274
	10 20 30 40 50 60 MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG 70 80 90 100 110 120 DERVKNGLNF MYEAPPGAKK ENKEKEETEG ETEYKFEWQK GAPREKYAKD DMNIRDQPFG 130 140 150 160 170 180 IQVRNVRCIK CHKWGHVNTD RECPLFGLSG INASSVPTDG SDAVLQIEGF WAIDAPLGAN 190 200 SGDEKQWVCT ETKCTGEKLD RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex." Hsieh J.J.-D., Zhou S., Chen L., Young D.B., Hayward S.D. Proc. Natl. Acad. Sci. U.S.A. 96:23-28(1999) [PubMed: 9874765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RBPJ; SAP30 AND HDAC2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	Chai K.X., Li L., Chao J., Chao L. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye, Lymph, Testis and Uterus.
[6]	"Epstein-Barr virus BamHi-a rightward transcript-encoded RPMS protein interacts with the CBF1-associated corepressor CIR to negatively regulate the activity of EBNA2 and NotchIC." Zhang J., Chen H., Weinmaster G., Hayward S.D. J. Virol. 75:2946-2956(2001) [PubMed: 11222720] [Abstract] Cited for: INTERACTION WITH EPSTEIN-BARR VIRUS RPMS1, SUBCELLULAR LOCATION.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"NKAP is a transcriptional repressor of notch signaling and is required for T cell development." Pajerowski A.G., Nguyen C., Aghajanian H., Shapiro M.J., Shapiro V.S. Immunity 30:696-707(2009) [PubMed: 19409814] [Abstract] Cited for: FUNCTION, INTERACTION WITH NKAP.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[11]	"Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome." Vaz Meirelles G., Ferreira Lanza D.C., da Silva J.C., Santana Bernachi J., Paes Leme A.F., Kobarg J. J. Proteome Res. 9:6298-6316(2010) [PubMed: 20873783] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH NEK6, PHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF098297 mRNA. Translation: AAD05243.1. U03644 mRNA. Translation: AAA17853.1. Frameshift. AK292389 mRNA. Translation: BAF85078.1. AC018470 Genomic DNA. Translation: AAY24216.1. BC015040 mRNA. No translation available. BC021175 mRNA. Translation: AAH21175.1. Sequence problems. BC038987 mRNA. Translation: AAH38987.1. Sequence problems. BC046098 mRNA. Translation: AAH46098.1.
IPI	IPI00743170. IPI00749452.
PIR	G01227.
RefSeq	NP_004873.3. NM_004882.3.
UniGene	Hs.632531.
3D structure databases
ProteinModelPortal	Q86X95.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86X95. 10 interactions.
MINT	MINT-207899.
STRING	Q86X95.
PTM databases
PhosphoSite	Q86X95.
Polymorphism databases
DMDM	74727790.
Proteomic databases
PRIDE	Q86X95.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000342016; ENSP00000339723; ENSG00000138433.
GeneID	9541.
KEGG	hsa:9541.
UCSC	uc002uim.1. human.
Organism-specific databases
CTD	9541.
GeneCards	GC02M175212.
H-InvDB	HIX0023923.
HGNC	HGNC:24217. CIR1.
HPA	HPA015784.
MIM	605228. gene.
neXtProt	NX_Q86X95.
PharmGKB	PA165696415.
GenAtlas	Search...
Phylogenomic databases
GeneTree	ENSGT00550000074692.
InParanoid	Q86X95.
OMA	SRSKHKK.
PhylomeDB	Q86X95.
Gene expression databases
ArrayExpress	Q86X95.
Bgee	Q86X95.
Genevestigator	Q86X95.
GermOnline	ENSG00000138433. Homo sapiens.
Family and domain databases
InterPro	IPR019339. CIR_N_dom. [Graphical view]
KO	K06066.
Pfam	PF10197. Cir_N. 1 hit. [Graphical view]
SMART	SM01083. Cir_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	35776.
SOURCE	Search...

Entry information

Entry name

CIR1_HUMAN

Accession

Primary (citable) accession number: Q86X95
Secondary accession number(s): A6NFI6 , A8K8M4, O95367, Q12804, Q4G1B9, Q6PJI4, Q8IWI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.