Reviewed, UniProtKB/Swiss-Prot Q86X95 (CIR_HUMAN)

Names and origin

Protein names

Recommended name:
    CBF1-interacting corepressor
Alternative name(s):
    Recepin

Gene names

Name:

CIR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May modulate splice site selection during alternative splicing of pre-mRNAs By similarity. Regulates transcription and acts as corepressor for RBPSUH. Recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC). Required for RBPSUH-mediated repression of transcription.
Subunit structure	Interacts with RP9, SNW1, SFRS1, SFRS2 and U2AF1 By similarity. Interacts with Epstein-Barr virus RPMS1. Component of the histone deacetylase complex. Interacts with RBPSUH, SAP30 and HDAC2.
Subcellular location	Nucleus speckle. Ref.1 Ref.6
Tissue specificity	Highly expressed in heart, brain, placenta, liver, skeletal muscle and pancreas. Ref.1
Sequence caution	The sequence AAA17853.1 differs from that shown. Reason: Frameshift at positions 135, 151, 177, 203, 261, 299, 391 and 410. The sequence AAH21175.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence. The sequence AAH38987.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Transcription Transcription regulation mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Ref.1 Inferred from direct assay. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription corepressor activity Ref.1 Traceable author statement. Source: ProtInc transcription factor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SFRS1	Q07955	1	EBI-627102,EBI-398920
SFRS2	Q01130	1	EBI-627102,EBI-627047
U2AF1	Q01081	2	EBI-627102,EBI-632461

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86X95-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86X95-2)

The sequence of this isoform differs from the canonical sequence as follows:
     162-202: GPSMHPSELI...NDPSQEYVAS → DAVLQIEGFW...KCTGEKLDRK
     203-450: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	CBF1-interacting corepressor		PRO_0000247984
Regions
	Region	1 – 121	121	Interaction with RBPSUH
	Region	204 – 232	29	Interaction with RP9 By similarity
	Motif	235 – 245	11	Nuclear localization signal Potential
	Motif	291 – 298	8	Nuclear localization signal Potential
	Compositional bias	214 – 368	155	Lys/Ser-rich
	Compositional bias	369 – 450	82	Arg/Ser-rich (RS domain)
Amino acid modifications
	Modified residue	202	1	Phosphoserine Ref.7 Ref.8
Natural variations
	Alternative sequence	162 – 202	41	GPSMH…EYVAS → DAVLQIEGFWAIDAPLGANS GDEKQWVCTETKCTGEKLDR K in isoform 2.		VSP_020091
	Alternative sequence	203 – 450	248	Missing in isoform 2.		VSP_020092
Experimental info
	Sequence conflict	48	1	K → R in AAD05243. Ref.1
	Sequence conflict	113	1	N → S in AAD05243. Ref.1
	Sequence conflict	172	1	A → G in AAA17853. Ref.2
	Sequence conflict	325	1	S → T in AAD05243. Ref.1
	Sequence conflict	325	1	S → T in AAA17853. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5D0C1CBD9C87569D	FASTA	450	52,313
	10 20 30 40 50 60 MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG 70 80 90 100 110 120 DERVKNGLNF MYEAPPGAKK ENKEKEETEG ETEYKFEWQK GAPREKYAKD DMNIRDQPFG 130 140 150 160 170 180 IQVRNVRCIK CHKWGHVNTD RECPLFGLSG INASSVPTDG SGPSMHPSEL IAEMRNSGFA 190 200 210 220 230 240 LKRNVLGRNL TANDPSQEYV ASEGEEDPEV EFLKSLTTKQ KQKLLRKLDR LEKKKKKKDR 250 260 270 280 290 300 KKKKFQKSRS KHKKHKSSSS SSSSSSSSSS TETSESSSES ESNNKEKKIQ RKKRKKNKCS 310 320 330 340 350 360 GHNNSDSEEK DKSKKRKLHE ELSSSHHNRE KAKEKPRFLK HESSREDSKW SHSDSDKKSR 370 380 390 400 410 420 THKHSPEKRG SERKEGSSRS HGREERSRRS RSRSPGSYKQ RETRKRAQRN PGEEQSRRND 430 440 450 SRSHGTDLYR GEKMYREHPG GTHTKVTQRE
	Isoform 2. Checksum: 6C16582B3E91B8DE	FASTA	202	23,274
	10 20 30 40 50 60 MGKSFANFMC KKDFHPASKS NIKKVWMAEQ KISYDKKKQE ELMQQYLKEQ ESYDNRLLMG 70 80 90 100 110 120 DERVKNGLNF MYEAPPGAKK ENKEKEETEG ETEYKFEWQK GAPREKYAKD DMNIRDQPFG 130 140 150 160 170 180 IQVRNVRCIK CHKWGHVNTD RECPLFGLSG INASSVPTDG SDAVLQIEGF WAIDAPLGAN 190 200 SGDEKQWVCT ETKCTGEKLD RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex." Hsieh J.J.-D., Zhou S., Chen L., Young D.B., Hayward S.D. Proc. Natl. Acad. Sci. U.S.A. 96:23-28(1999) [PubMed: 9874765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH RBPSUH; SAP30 AND HDAC2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	Chai K.X., Li L., Chao J., Chao L. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye, Lymph, Testis and Uterus.
[6]	"Epstein-Barr virus BamHi-a rightward transcript-encoded RPMS protein interacts with the CBF1-associated corepressor CIR to negatively regulate the activity of EBNA2 and NotchIC." Zhang J., Chen H., Weinmaster G., Hayward S.D. J. Virol. 75:2946-2956(2001) [PubMed: 11222720] [Abstract] Cited for: INTERACTION WITH EPSTEIN-BARR VIRUS RPMS1, SUBCELLULAR LOCATION.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF098297 mRNA. Translation: AAD05243.1. U03644 mRNA. Translation: AAA17853.1. Frameshift. AK292389 mRNA. Translation: BAF85078.1. AC018470 Genomic DNA. Translation: AAY24216.1. BC015040 mRNA. Translation: AAH15040.1. BC021175 mRNA. Translation: AAH21175.1. Sequence problems. BC038987 mRNA. Translation: AAH38987.1. Sequence problems. BC046098 mRNA. Translation: AAH46098.1.
IPI	IPI00743170. IPI00749452.
PIR	G01227.
RefSeq	NP_004873.3.
UniGene	Hs.632531
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86X95. 7 interactions.
PTM databases
PhosphoSite	Q86X95.
Proteomic databases
PRIDE	Q86X95.
Genome annotation databases
Ensembl	ENSG00000138433. Homo sapiens. [Contig view]
GeneID	9541.
KEGG	hsa:9541.
Organism-specific databases
GeneCards	GC02M174921.
HPA	HPA015784.
MIM	605228. gene.
Phylogenomic databases
HOGENOM	Q86X95.
HOVERGEN	Q86X95.
OMA	Q86X95. SRSKHKK.
Gene expression databases
ArrayExpress	Q86X95.
Bgee	Q86X95.
GermOnline	ENSG00000138433. Homo sapiens.
Family and domain databases
InterPro	IPR019339. CBF1-interact_co-repress_CIR_N. [Graphical view]
Pfam	PF10197. Cir_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	35776.
SOURCE	Search...

Entry information

Entry name

CIR_HUMAN

Accession

Primary (citable) accession number: Q86X95
Secondary accession number(s): A6NFI6 , A8K8M4, O95367, Q12804, Q4G1B9, Q6PJI4, Q8IWI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)