Reviewed, UniProtKB/Swiss-Prot Q86X29 (LSR_HUMAN)

Names and origin

Protein names

Recommended name:
    Lipolysis-stimulated lipoprotein receptor

Gene names

Name:	LSR
Synonyms:	LISCH

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells By similarity.
Subunit structure	Homotrimer or homotetramer By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the immunoglobulin superfamily. LISCH7 family. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Caution	In contrast to the rodent orthologous protein, it is longer in N-terminus and no signal sequence is detected by any prediction method.
Sequence caution	The sequence AAB51178.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAB58317.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Chylomicron LDL Membrane VLDL
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Immunoglobulin domain Transmembrane
Molecular function	Receptor
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development Inferred from sequence or structural similarity. Source: HGNC liver development Inferred from sequence or structural similarity. Source: HGNC
Cellular component	Golgi apparatus Inferred from direct assay. Source: HPA chylomicron Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA very-low-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86X29-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86X29-2)

The sequence of this isoform differs from the canonical sequence as follows:
     52-88: Missing.
     366-386: Missing.

Isoform 3 (identifier: Q86X29-3)

The sequence of this isoform differs from the canonical sequence as follows:
     240-258: Missing.
     386-386: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Lipolysis-stimulated lipoprotein receptor		PRO_0000245308
Regions
	Topological domain	1 – 259	259	Extracellular Potential
	Transmembrane	260 – 280	21	Potential
	Topological domain	281 – 649	369	Cytoplasmic Potential
	Domain	86 – 234	149	Ig-like V-type
	Compositional bias	280 – 304	25	Cys-rich
Amino acid modifications
	Modified residue	185	1	Phosphothreonine By similarity
	Modified residue	309	1	Phosphotyrosine Ref.6
	Modified residue	328	1	Phosphotyrosine Ref.6
	Modified residue	364	1	Phosphoserine By similarity
	Modified residue	372	1	Phosphotyrosine Ref.10
	Modified residue	406	1	Phosphotyrosine Ref.6 Ref.10
	Modified residue	436	1	Phosphoserine By similarity
	Modified residue	493	1	Phosphoserine Ref.8
	Modified residue	528	1	Phosphoserine Ref.7
	Modified residue	530	1	Phosphoserine Ref.7 Ref.9
	Modified residue	535	1	Phosphotyrosine Ref.6
	Modified residue	551	1	Phosphotyrosine Ref.6
	Modified residue	586	1	Phosphotyrosine Ref.6
	Modified residue	615	1	Phosphotyrosine Ref.6
	Modified residue	646	1	Phosphoserine Ref.5
	Disulfide bond	111 ↔ 218		By similarity
Natural variations
	Alternative sequence	52 – 88	37	Missing in isoform 2.		VSP_019691
	Alternative sequence	240 – 258	19	Missing in isoform 3.		VSP_019692
	Alternative sequence	366 – 386	21	Missing in isoform 2.		VSP_019693
	Alternative sequence	386	1	Missing in isoform 3.		VSP_019694
	Natural variant	363	1	S → N: dbSNP rs34259399. Ref.2		VAR_049902
Experimental info
	Sequence conflict	259	1	D → G in AAB51178. Ref.3
	Sequence conflict	308	1	L → R in AAB51178. Ref.3
	Sequence conflict	430	1	A → G in AAB51178. Ref.3
	Sequence conflict	518	1	G → GR in BAC86714. Ref.2
	Sequence conflict	639	1	N → D in AAB51178. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 4. Checksum: 01E8F1CE0197CE9B	FASTA	649	71,439
	10 20 30 40 50 60 MQQDGLGVGT RNGSGKGRSV HPSWPWCAPR PLRYFGRDAR ARRAQTAAMA LLAGGLSRGL 70 80 90 100 110 120 GSHPAAAGRD AVVFVWLLLS TWCTAPARAI QVTVSNPYHV VILFQPVTLP CTYQMTSTPT 130 140 150 160 170 180 QPIVIWKYKS FCRDRIADAF SPASVDNQLN AQLAAGNPGY NPYVECQDSV RTVRVVATKQ 190 200 210 220 230 240 GNAVTLGDYY QGRRITITGN ADLTFDQTAW GDSGVYYCSV VSAQDLQGNN EAYAELIVLG 250 260 270 280 290 300 RTSGVAELLP GFQAGPIEDW LFVVVVCLAA FLIFLLLGIC WCQCCPHTCC CYVRCPCCPD 310 320 330 340 350 360 KCCCPEALYA AGKAATSGVP SIYAPSTYAH LSPAKTPPPP AMIPMGPAYN GYPGGYPGDV 370 380 390 400 410 420 DRSSSAGGQG SYVPLLRDTD SSVASEVRSG YRIQASQQDD SMRVLYYMEK ELANFDPSRP 430 440 450 460 470 480 GPPSGRVERA MSEVTSLHED DWRSRPSRGP ALTPIRDEEW GGHSPRSPRG WDQEPAREQA 490 500 510 520 530 540 GGGWRARRPR ARSVDALDDL TPPSTAESGS RSPTSNGGRS RAYMPPRSRS RDDLYDQDDS 550 560 570 580 590 600 RDFPRSRDPH YDDFRSRERP PADPRSHHHR TRDPRDNGSR SGDLPYDGRL LEEAVRKKGS 610 620 630 640 EERRRPHKEE EEEAYYPPAP PPYSETDSQA SRERRLKKNL ALSRESLVV
	Isoform 2. Checksum: E254FA92B362D418	FASTA	591	65,587
	10 20 30 40 50 60 MQQDGLGVGT RNGSGKGRSV HPSWPWCAPR PLRYFGRDAR ARRAQTAAMA LAIQVTVSNP 70 80 90 100 110 120 YHVVILFQPV TLPCTYQMTS TPTQPIVIWK YKSFCRDRIA DAFSPASVDN QLNAQLAAGN 130 140 150 160 170 180 PGYNPYVECQ DSVRTVRVVA TKQGNAVTLG DYYQGRRITI TGNADLTFDQ TAWGDSGVYY 190 200 210 220 230 240 CSVVSAQDLQ GNNEAYAELI VLGRTSGVAE LLPGFQAGPI EDWLFVVVVC LAAFLIFLLL 250 260 270 280 290 300 GICWCQCCPH TCCCYVRCPC CPDKCCCPEA LYAAGKAATS GVPSIYAPST YAHLSPAKTP 310 320 330 340 350 360 PPPAMIPMGP AYNGYPGGYP GDVDRSSSVR SGYRIQASQQ DDSMRVLYYM EKELANFDPS 370 380 390 400 410 420 RPGPPSGRVE RAMSEVTSLH EDDWRSRPSR GPALTPIRDE EWGGHSPRSP RGWDQEPARE 430 440 450 460 470 480 QAGGGWRARR PRARSVDALD DLTPPSTAES GSRSPTSNGG RSRAYMPPRS RSRDDLYDQD 490 500 510 520 530 540 DSRDFPRSRD PHYDDFRSRE RPPADPRSHH HRTRDPRDNG SRSGDLPYDG RLLEEAVRKK 550 560 570 580 590 GSEERRRPHK EEEEEAYYPP APPPYSETDS QASRERRLKK NLALSRESLV V
	Isoform 3. Checksum: 32D531E33D09464E	FASTA	629	69,429
	10 20 30 40 50 60 MQQDGLGVGT RNGSGKGRSV HPSWPWCAPR PLRYFGRDAR ARRAQTAAMA LLAGGLSRGL 70 80 90 100 110 120 GSHPAAAGRD AVVFVWLLLS TWCTAPARAI QVTVSNPYHV VILFQPVTLP CTYQMTSTPT 130 140 150 160 170 180 QPIVIWKYKS FCRDRIADAF SPASVDNQLN AQLAAGNPGY NPYVECQDSV RTVRVVATKQ 190 200 210 220 230 240 GNAVTLGDYY QGRRITITGN ADLTFDQTAW GDSGVYYCSV VSAQDLQGNN EAYAELIVLD 250 260 270 280 290 300 WLFVVVVCLA AFLIFLLLGI CWCQCCPHTC CCYVRCPCCP DKCCCPEALY AAGKAATSGV 310 320 330 340 350 360 PSIYAPSTYA HLSPAKTPPP PAMIPMGPAY NGYPGGYPGD VDRSSSAGGQ GSYVPLLRDT 370 380 390 400 410 420 DSSVASVRSG YRIQASQQDD SMRVLYYMEK ELANFDPSRP GPPSGRVERA MSEVTSLHED 430 440 450 460 470 480 DWRSRPSRGP ALTPIRDEEW GGHSPRSPRG WDQEPAREQA GGGWRARRPR ARSVDALDDL 490 500 510 520 530 540 TPPSTAESGS RSPTSNGGRS RAYMPPRSRS RDDLYDQDDS RDFPRSRDPH YDDFRSRERP 550 560 570 580 590 600 PADPRSHHHR TRDPRDNGSR SGDLPYDGRL LEEAVRKKGS EERRRPHKEE EEEAYYPPAP 610 620 PPYSETDSQA SRERRLKKNL ALSRESLVV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional analysis of LISCH." Teramoto T., Thorgeirsson S.S. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), VARIANT ASN-363. Tissue: Amygdala.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[5]	"Global phosphoproteome of HT-29 human colon adenocarcinoma cells." Kim J.-E., Tannenbaum S.R., White F.M. J. Proteome Res. 4:1339-1346(2005) [PubMed: 16083285] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-646, MASS SPECTROMETRY.
[6]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-309; TYR-328; TYR-406; TYR-535; TYR-551; TYR-586 AND TYR-615, MASS SPECTROMETRY.
[7]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-528 AND SER-530, MASS SPECTROMETRY.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-493, MASS SPECTROMETRY.
[9]	"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361(2008) [PubMed: 18318008] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530, MASS SPECTROMETRY. Tissue: Liver.
[10]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-372 AND TYR-406, MASS SPECTROMETRY. Tissue: Mammary epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF130366 mRNA. Translation: AAD22101.1. AK126834 mRNA. Translation: BAC86714.1. AC002128 Genomic DNA. Translation: AAB58317.1. Sequence problems. AD000684 Genomic DNA. Translation: AAB51178.1. Sequence problems. BC047376 mRNA. Translation: AAH47376.2.
IPI	IPI00328218. IPI00409640. IPI00941735.
RefSeq	NP_057009.3. NP_991403.1. NP_991404.1.
UniGene	Hs.466507
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86X29. 3 interactions.
STRING	Q86X29.
PTM databases
PhosphoSite	Q86X29.
Proteomic databases
PRIDE	Q86X29.
Genome annotation databases
Ensembl	ENST00000347609; ENSP00000262627; ENSG00000105699; Homo sapiens. [Genome view] ENST00000354900; ENSP00000346976; ENSG00000105699; Homo sapiens. [Genome view] ENST00000360798; ENSP00000354034; ENSG00000105699; Homo sapiens. [Genome view] ENST00000361790; ENSP00000354575; ENSG00000105699; Homo sapiens. [Genome view] ENST00000427250; ENSP00000394479; ENSG00000105699; Homo sapiens. [Genome view] ENST00000443983; ENSP00000389545; ENSG00000105699; Homo sapiens. [Genome view]
GeneID	51599.
KEGG	hsa:51599.
UCSC	uc002nyl.1. human. uc002nyo.1. human. uc002nyp.1. human.
Organism-specific databases
CTD	51599.
GeneCards	GC19P040432.
HGNC	HGNC:29572. LSR.
HPA	HPA007270.
PharmGKB	PA142671504.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q86X29.
HOVERGEN	Q86X29.
OMA	WRSRPSR.
Gene expression databases
ArrayExpress	Q86X29.
Bgee	Q86X29.
CleanEx	HS_LSR.
Genevestigator	Q86X29.
GermOnline	ENSG00000105699. Homo sapiens.
Family and domain databases
InterPro	IPR007110. Ig-like. IPR003599. Ig_sub. IPR008664. LISCH7. [Graphical view]
Pfam	PF05624. LSR. 1 hit. [Graphical view]
SMART	SM00409. IG. 1 hit. [Graphical view]
PROSITE	PS50835. IG_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	55459.

Entry information

Entry name

LSR_HUMAN

Accession

Primary (citable) accession number: Q86X29
Secondary accession number(s): O00112 , O00426, Q6ZT80, Q9UQL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 69 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.