Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q86X10 (RLGPB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ral GTPase-activating protein subunit beta
Alternative name(s):
p170
Gene names
Name:RALGAPB
Synonyms:KIAA1219
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-catalytic subunit of the heterodimeric RalGAP1 and RalGAP2 complexes which act as GTPase activators for the Ras-like small GTPases RALA and RALB By similarity.

Subunit structure

Component of the heterodimeric RalGAP1 complex with RALGAPA1 and of the heterodimeric RalGAP2 complex with RALGAPA2. Heterodimerization is required for activity By similarity.

Tissue specificity

Highly expressed in brain, mostly in amygdala.

Sequence similarities

Contains 1 Rap-GAP domain.

Sequence caution

The sequence BAA86533.3 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAC85649.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAB61346.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAD39096.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86X10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86X10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.
     1233-1233: E → EE
Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Isoform 3 (identifier: Q86X10-3)

The sequence of this isoform differs from the canonical sequence as follows:
     749-752: Missing.
Note: May be due to a competing acceptor splice site. No experimental confirmation available.
Isoform 4 (identifier: Q86X10-4)

The sequence of this isoform differs from the canonical sequence as follows:
     943-1473: Missing.
Note: Splicing acceptor site is not canonical. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14941494Ral GTPase-activating protein subunit beta
PRO_0000056756

Regions

Domain1149 – 1392244Rap-GAP

Amino acid modifications

Modified residue7341Phosphothreonine Ref.10

Natural variations

Alternative sequence1 – 222222Missing in isoform 2.
VSP_009693
Alternative sequence749 – 7524Missing in isoform 3.
VSP_009694
Alternative sequence943 – 1473531Missing in isoform 4.
VSP_009695
Alternative sequence12331E → EE in isoform 2.
VSP_009696

Experimental info

Sequence conflict3261P → S in CAD39096. Ref.1
Sequence conflict10721Y → G in AAH18668. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 5FEDBB0CB269F8CE

FASTA1,494166,799
        10         20         30         40         50         60 
MYSEWRSLHL VIQNDQGHTS VLHSYPESVG REVANAVVRP LGQVLGTPSV AGSENLLKTD 

        70         80         90        100        110        120 
KEVKWTMEVI CYGLTLPLDG ETVKYCVDVY TDWIMALVLP KDSIPLPVIK EPNQYVQTIL 

       130        140        150        160        170        180 
KHLQNLFVPR QEQGSSQIRL CLQVLRAIQK LARESSLMAR ETWEVLLLFL LQINDILLAP 

       190        200        210        220        230        240 
PTVQGGIAEN LAEKLIGVLF EVWLLACTRC FPTPPYWKTA KEMVANWRHH PAVVEQWSKV 

       250        260        270        280        290        300 
ICALTSRLLR FTYGPSFPAF KVPDEDASLI PPEMDNECVA QTWFRFLHML SNPVDLSNPA 

       310        320        330        340        350        360 
IISSTPKFQE QFLNVSGMPQ ELNQYPCLKH LPQIFFRAMR GISCLVDAFL GISRPRSDSA 

       370        380        390        400        410        420 
PPTPVNRLSM PQSAAVSTTP PHNRRHRAVT VNKATMKTST VSTAHASKVQ HQTSSTSPLS 

       430        440        450        460        470        480 
SPNQTSSEPR PLPAPRRPKV NSILNLFGSW LFDAAFVHCK LHNGINRDSS MTAITTQASM 

       490        500        510        520        530        540 
EFRRKGSQMS TDTMVSNPMF DASEFPDNYE AGRAEACGTL CRIFCSKKTG EEILPAYLSR 

       550        560        570        580        590        600 
FYMLLIQGLQ INDYVCHPVL ASVILNSPPL FCCDLKGIDV VVPYFISALE TILPDRELSK 

       610        620        630        640        650        660 
FKSYVNPTEL RRSSINILLS LLPLPHHFGT VKSEVVLEGK FSNDDSSSYD KPITFLSLKL 

       670        680        690        700        710        720 
RLVNILIGAL QTETDPNNTQ MILGAMLNIV QDSALLEAIG CQMEMGGGEN NLKSHSRTNS 

       730        740        750        760        770        780 
GISSASGGST EPTTPDSERP AQALLRDYAL NTDSAAGLLI RSIHLVTQRL NSQWRQDMSI 

       790        800        810        820        830        840 
SLAALELLSG LAKVKVMVDS GDRKRAISSV CTYIVYQCSR PAPLHSRDLH SMIVAAFQCL 

       850        860        870        880        890        900 
CVWLTEHPDM LDEKDCLKEV LEIVELGISG SKSKNNEQEV KYKGDKEPNP ASMRVKDAAE 

       910        920        930        940        950        960 
ATLTCIMQLL GAFPSPSGPA SPCSLVNETT LIKYSRLPTI NKHSFRYFVL DNSVILAMLE 

       970        980        990       1000       1010       1020 
QPLGNEQNDF FPSVTVLVRG MSGRLAWAQQ LCLLPRGAKA NQKLFVPEPR PVPKNDVGFK 

      1030       1040       1050       1060       1070       1080 
YSVKHRPFPE EVDKIPFVKA DLSIPDLHEI VTEELEERHE KLRSGMAQQI AYEIHLEQQS 

      1090       1100       1110       1120       1130       1140 
EEELQKRSFP DPVTDCKPPP PAQEFQTARL FLSHFGFLSL EALKEPANSR LPPHLIALDS 

      1150       1160       1170       1180       1190       1200 
TIPGFFDDIG YLDLLPCRPF DTVFIFYMKP GQKTNQEILK NVESSRTVQP HFLEFLLSLG 

      1210       1220       1230       1240       1250       1260 
WSVDVGRHPG WTGHVSTSWS INCCDDGEGS QQEVISSEDI GASIFNGQKK VLYYADALTE 

      1270       1280       1290       1300       1310       1320 
IAFVVPSPVE SLTDSLESNI SDQDSDSNMD LMPGILKQPS LTLELFPNHT DNLNSSQRLS 

      1330       1340       1350       1360       1370       1380 
PSSRMRKLPQ GRPVPPLGPE TRVSVVWVER YDDIENFPLS ELMTEISTGV ETTANSSTSL 

      1390       1400       1410       1420       1430       1440 
RSTTLEKEVP VIFIHPLNTG LFRIKIQGAT GKFNMVIPLV DGMIVSRRAL GFLVRQTVIN 

      1450       1460       1470       1480       1490 
ICRRKRLESD SYSPPHVRRK QKITDIVNKY RNKQLEPEFY TSLFQEVGLK NCSS 

« Hide

Isoform 2 [UniParc].

Checksum: 024E924F27623CC2
Show »

FASTA1,273141,866
Isoform 3 [UniParc].

Checksum: A563AECFFF89DEDD
Show »

FASTA1,490166,399
Isoform 4 [UniParc].

Checksum: 13AF758962E013A4
Show »

FASTA963106,838

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.
DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Brain.
[2]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]Ohara O., Nagase T., Kikuno R.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Melanoma.
[5]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain, Lung and Testis.
[7]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-1494 (ISOFORM 4).
Tissue: Tongue.
[8]"Adipocytes contain a novel complex similar to the tuberous sclerosis complex."
Gridley S., Chavez J.A., Lane W.S., Lienhard G.E.
Cell. Signal. 18:1626-1632(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RALGAPA2.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-734, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB033045 mRNA. Translation: BAA86533.3. Different initiation.
AL834436 mRNA. Translation: CAD39096.1. Different initiation.
AL132998 mRNA. Translation: CAB61346.1. Different initiation.
AL035419, AL049868 Genomic DNA. Translation: CAM27455.1.
AL049868, AL035419 Genomic DNA. Translation: CAM28344.1.
BC010916 mRNA. Translation: AAH10916.2.
BC018668 mRNA. Translation: AAH18668.1.
BC047482 mRNA. Translation: AAH47482.1.
AK123576 mRNA. Translation: BAC85649.1. Different initiation.
RefSeqNP_001269846.1. NM_001282917.1.
NP_001269847.1. NM_001282918.1.
NP_065069.1. NM_020336.3.
UniGeneHs.598850.

3D structure databases

ProteinModelPortalQ86X10.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121405. 3 interactions.
IntActQ86X10. 2 interactions.

PTM databases

PhosphoSiteQ86X10.

Polymorphism databases

DMDM45477126.

Proteomic databases

PaxDbQ86X10.
PRIDEQ86X10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000262879; ENSP00000262879; ENSG00000170471. [Q86X10-1]
ENST00000397038; ENSP00000380231; ENSG00000170471. [Q86X10-2]
ENST00000397040; ENSP00000380233; ENSG00000170471. [Q86X10-1]
GeneID57148.
KEGGhsa:57148.
UCSCuc002xiw.3. human. [Q86X10-1]
uc002xiz.3. human. [Q86X10-2]

Organism-specific databases

CTD57148.
GeneCardsGC20P037102.
H-InvDBHIX0203031.
HGNCHGNC:29221. RALGAPB.
HPAHPA051454.
neXtProtNX_Q86X10.
PharmGKBPA165392608.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG293167.
HOVERGENHBG052199.
InParanoidQ86X10.
OMAVIKEPNQ.
OrthoDBEOG757CWN.
PhylomeDBQ86X10.
TreeFamTF324460.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpressQ86X10.
BgeeQ86X10.
CleanExHS_KIAA1219.
GenevestigatorQ86X10.

Family and domain databases

InterProIPR000331. Rap_GAP_dom.
[Graphical view]
PROSITEPS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKIAA1219.
GenomeRNAi57148.
NextBio63107.
PROQ86X10.

Entry information

Entry nameRLGPB_HUMAN
AccessionPrimary (citable) accession number: Q86X10
Secondary accession number(s): A2A2E8 expand/collapse secondary AC list , Q5TG31, Q8N3D1, Q8WWC0, Q9H3X8, Q9UJR1, Q9ULK1, Q9Y3G9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM