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Protein

E3 ubiquitin-protein ligase TRIM69

Gene

TRIM69

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 82RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Apoptosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:G66-32483-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM69 (EC:6.3.2.-)
Alternative name(s):
RFP-like domain-containing protein trimless
RING finger protein 36
Tripartite motif-containing protein 69
Gene namesi
Name:TRIM69
Synonyms:RNF36
ORF Names:HSD-34, HSD34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:17857. TRIM69.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • nuclear speck Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61C → A: Loss of E3 ubiquitin-protein ligase activity; when associated with A-64. 1 Publication1
Mutagenesisi64C → A: Loss of E3 ubiquitin-protein ligase activity; when associated with A-61. 1 Publication1

Organism-specific databases

DisGeNETi140691.
OpenTargetsiENSG00000185880.
ENSG00000278211.
PharmGKBiPA162407010.

Polymorphism and mutation databases

BioMutaiTRIM69.
DMDMi229462760.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782361 – 500E3 ubiquitin-protein ligase TRIM69Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is necessary for nuclear localization (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ86WT6.
PeptideAtlasiQ86WT6.
PRIDEiQ86WT6.
TopDownProteomicsiQ86WT6-2. [Q86WT6-2]

PTM databases

iPTMnetiQ86WT6.
PhosphoSitePlusiQ86WT6.

Expressioni

Gene expression databases

BgeeiENSG00000185880.
CleanExiHS_TRIM69.
ExpressionAtlasiQ86WT6. baseline and differential.
GenevisibleiQ86WT6. HS.

Organism-specific databases

HPAiHPA028894.

Interactioni

Subunit structurei

Interacts with PML.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ASNSP082433EBI-749955,EBI-722989
ATP5G1P054963EBI-749955,EBI-10194585
DVL1Q86TH33EBI-749955,EBI-10185025
FAM110AQ9BQ893EBI-749955,EBI-1752811
FKBP1BP681063EBI-749955,EBI-6693977
HAUS1Q96CS23EBI-749955,EBI-2514791
HGSO149644EBI-11525489,EBI-740220
IYDQ6PHW03EBI-749955,EBI-10253668
KRT17F5GWP83EBI-749955,EBI-10177711
LARP1BQ659C43EBI-749955,EBI-3940258
MAGEA4Q1RN333EBI-749955,EBI-10194128
MID2Q9UJV3-24EBI-11525489,EBI-10172526
NEK8Q86SG63EBI-749955,EBI-1752987
PPP1R18Q6NYC83EBI-749955,EBI-2557469
RECKQ6P9E23EBI-749955,EBI-10253121
RSPH14Q9UHP63EBI-749955,EBI-748350
SF1Q156373EBI-749955,EBI-744603
SF3A3Q128743EBI-749955,EBI-1051880
SYNE4Q8N2053EBI-749955,EBI-7131783
TRIM44Q96DX73EBI-749955,EBI-8787399
ZNF483Q6P0883EBI-749955,EBI-10196963

Protein-protein interaction databases

BioGridi126654. 36 interactors.
IntActiQ86WT6. 34 interactors.
MINTiMINT-1465642.
STRINGi9606.ENSP00000332284.

Structurei

Secondary structure

1500
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 148Combined sources5
Helixi153 – 260Combined sources108
Helixi266 – 271Combined sources6
Helixi273 – 287Combined sources15
Helixi298 – 301Combined sources4
Helixi305 – 318Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NQJX-ray2.15A/B/C143-321[»]
ProteinModelPortaliQ86WT6.
SMRiQ86WT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini305 – 500B30.2/SPRYPROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 152Necessary for nuclear localizationBy similarityAdd BLAST152

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili161 – 255Sequence analysisAdd BLAST95

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localization and aggregation.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 82RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOVERGENiHBG001357.
InParanoidiQ86WT6.
KOiK12034.
OMAiFFTEELT.
OrthoDBiEOG091G05W2.
PhylomeDBiQ86WT6.
TreeFamiTF342569.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86WT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSTNPSSN IDPGDYVEMN DSITHLPSKV VIQDITMELH CPLCNDWFRD
60 70 80 90 100
PLMLSCGHNF CEACIQDFWR LQAKETFCPE CKMLCQYNNC TFNPVLDKLV
110 120 130 140 150
EKIKKLPLLK GHPQCPEHGE NLKLFSKPDG KLICFQCKDA RLSVGQSKEF
160 170 180 190 200
LQISDAVHFF TEELAIQQGQ LETTLKELQT LRNMQKEAIA AHKENKLHLQ
210 220 230 240 250
QHVSMEFLKL HQFLHSKEKD ILTELREEGK ALNEEMELNL SQLQEQCLLA
260 270 280 290 300
KDMLVSIQAK TEQQNSFDFL KDITTLLHSL EQGMKVLATR ELISRKLNLG
310 320 330 340 350
QYKGPIQYMV WREMQDTLCP GLSPLTLDPK TAHPNLVLSK SQTSVWHGDI
360 370 380 390 400
KKIMPDDPER FDSSVAVLGS RGFTSGKWYW EVEVAKKTKW TVGVVRESII
410 420 430 440 450
RKGSCPLTPE QGFWLLRLRN QTDLKALDLP SFSLTLTNNL DKVGIYLDYE
460 470 480 490 500
GGQLSFYNAK TMTHIYTFSN TFMEKLYPYF CPCLNDGGEN KEPLHILHPQ
Length:500
Mass (Da):57,419
Last modified:May 5, 2009 - v2
Checksum:iE9957652895213B3
GO
Isoform 2 (identifier: Q86WT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-161: Missing.

Show »
Length:341
Mass (Da):39,254
Checksum:i36DF6708DB5DBF7B
GO
Isoform 3 (identifier: Q86WT6-3) [UniParc]FASTAAdd to basket
Also known as: Trimless alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Show »
Length:296
Mass (Da):34,033
Checksum:iFD94B8F14B04945B
GO
Isoform 4 (identifier: Q86WT6-4) [UniParc]FASTAAdd to basket
Also known as: Trimless beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.
     255-271: Missing.

Show »
Length:279
Mass (Da):32,068
Checksum:i732E356FEF439FF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469S → G in AAQ75551 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05722612D → N.Corresponds to variant rs2444007dbSNPEnsembl.1
Natural variantiVAR_05722715D → N.1 PublicationCorresponds to variant rs2470911dbSNPEnsembl.1
Natural variantiVAR_03070431V → A.Corresponds to variant rs3759880dbSNPEnsembl.1
Natural variantiVAR_057228104K → R.Corresponds to variant rs17588988dbSNPEnsembl.1
Natural variantiVAR_057229161T → M.1 PublicationCorresponds to variant rs3100139dbSNPEnsembl.1
Natural variantiVAR_057230190A → V.Corresponds to variant rs3759880dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232001 – 204Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST204
Alternative sequenceiVSP_0232013 – 161Missing in isoform 2. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_023202255 – 271Missing in isoform 4. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302088 mRNA. Translation: AAL55810.1.
AF302089 mRNA. Translation: AAL55811.1.
DQ232883 mRNA. Translation: ABB18376.1.
AY305385 mRNA. Translation: AAQ75551.1.
AC122108 Genomic DNA. No translation available.
BC024199 mRNA. Translation: AAH24199.1. Different termination.
BC033314 mRNA. Translation: AAH33314.1.
BC047945 mRNA. Translation: AAH47945.1.
CCDSiCCDS10114.1. [Q86WT6-2]
CCDS32220.1. [Q86WT6-1]
CCDS73719.1. [Q86WT6-3]
CCDS76746.1. [Q86WT6-4]
RefSeqiNP_001288073.1. NM_001301144.1. [Q86WT6-3]
NP_001288074.1. NM_001301145.1. [Q86WT6-4]
NP_001288075.1. NM_001301146.1.
NP_542783.2. NM_080745.4. [Q86WT6-2]
NP_892030.3. NM_182985.4. [Q86WT6-1]
UniGeneiHs.745248.

Genome annotation databases

EnsembliENST00000329464; ENSP00000332284; ENSG00000185880. [Q86WT6-1]
ENST00000338264; ENSP00000342922; ENSG00000185880. [Q86WT6-2]
ENST00000558329; ENSP00000453332; ENSG00000185880. [Q86WT6-4]
ENST00000559390; ENSP00000453177; ENSG00000185880. [Q86WT6-1]
ENST00000560442; ENSP00000453549; ENSG00000185880. [Q86WT6-3]
ENST00000618398; ENSP00000479720; ENSG00000278211. [Q86WT6-2]
ENST00000631740; ENSP00000488341; ENSG00000278211. [Q86WT6-4]
ENST00000633106; ENSP00000488084; ENSG00000278211. [Q86WT6-3]
GeneIDi140691.
KEGGihsa:140691.
UCSCiuc001zuf.3. human. [Q86WT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302088 mRNA. Translation: AAL55810.1.
AF302089 mRNA. Translation: AAL55811.1.
DQ232883 mRNA. Translation: ABB18376.1.
AY305385 mRNA. Translation: AAQ75551.1.
AC122108 Genomic DNA. No translation available.
BC024199 mRNA. Translation: AAH24199.1. Different termination.
BC033314 mRNA. Translation: AAH33314.1.
BC047945 mRNA. Translation: AAH47945.1.
CCDSiCCDS10114.1. [Q86WT6-2]
CCDS32220.1. [Q86WT6-1]
CCDS73719.1. [Q86WT6-3]
CCDS76746.1. [Q86WT6-4]
RefSeqiNP_001288073.1. NM_001301144.1. [Q86WT6-3]
NP_001288074.1. NM_001301145.1. [Q86WT6-4]
NP_001288075.1. NM_001301146.1.
NP_542783.2. NM_080745.4. [Q86WT6-2]
NP_892030.3. NM_182985.4. [Q86WT6-1]
UniGeneiHs.745248.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NQJX-ray2.15A/B/C143-321[»]
ProteinModelPortaliQ86WT6.
SMRiQ86WT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126654. 36 interactors.
IntActiQ86WT6. 34 interactors.
MINTiMINT-1465642.
STRINGi9606.ENSP00000332284.

PTM databases

iPTMnetiQ86WT6.
PhosphoSitePlusiQ86WT6.

Polymorphism and mutation databases

BioMutaiTRIM69.
DMDMi229462760.

Proteomic databases

PaxDbiQ86WT6.
PeptideAtlasiQ86WT6.
PRIDEiQ86WT6.
TopDownProteomicsiQ86WT6-2. [Q86WT6-2]

Protocols and materials databases

DNASUi140691.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329464; ENSP00000332284; ENSG00000185880. [Q86WT6-1]
ENST00000338264; ENSP00000342922; ENSG00000185880. [Q86WT6-2]
ENST00000558329; ENSP00000453332; ENSG00000185880. [Q86WT6-4]
ENST00000559390; ENSP00000453177; ENSG00000185880. [Q86WT6-1]
ENST00000560442; ENSP00000453549; ENSG00000185880. [Q86WT6-3]
ENST00000618398; ENSP00000479720; ENSG00000278211. [Q86WT6-2]
ENST00000631740; ENSP00000488341; ENSG00000278211. [Q86WT6-4]
ENST00000633106; ENSP00000488084; ENSG00000278211. [Q86WT6-3]
GeneIDi140691.
KEGGihsa:140691.
UCSCiuc001zuf.3. human. [Q86WT6-1]

Organism-specific databases

CTDi140691.
DisGeNETi140691.
GeneCardsiTRIM69.
HGNCiHGNC:17857. TRIM69.
HPAiHPA028894.
MIMi616017. gene.
neXtProtiNX_Q86WT6.
OpenTargetsiENSG00000185880.
ENSG00000278211.
PharmGKBiPA162407010.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG410XWXM. LUCA.
GeneTreeiENSGT00760000118838.
HOVERGENiHBG001357.
InParanoidiQ86WT6.
KOiK12034.
OMAiFFTEELT.
OrthoDBiEOG091G05W2.
PhylomeDBiQ86WT6.
TreeFamiTF342569.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:G66-32483-MONOMER.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii140691.
PROiQ86WT6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000185880.
CleanExiHS_TRIM69.
ExpressionAtlasiQ86WT6. baseline and differential.
GenevisibleiQ86WT6. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00589. PRY. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRI69_HUMAN
AccessioniPrimary (citable) accession number: Q86WT6
Secondary accession number(s): A8MX03
, Q309B0, Q4G1A5, Q6W897, Q8IYY3, Q8WY16, Q8WY17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.