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Protein

E3 ubiquitin-protein ligase TRIM69

Gene

TRIM69

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 82RING-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processApoptosis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TRIM69 (EC:2.3.2.27)
Alternative name(s):
RFP-like domain-containing protein trimless
RING finger protein 36
RING-type E3 ubiquitin transferase TRIM69Curated
Tripartite motif-containing protein 69
Gene namesi
Name:TRIM69
Synonyms:RNF36
ORF Names:HSD-34, HSD34
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

EuPathDBiHostDB:ENSG00000185880.12
HGNCiHGNC:17857 TRIM69
MIMi616017 gene
neXtProtiNX_Q86WT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61C → A: Loss of E3 ubiquitin-protein ligase activity; when associated with A-64. 1 Publication1
Mutagenesisi64C → A: Loss of E3 ubiquitin-protein ligase activity; when associated with A-61. 1 Publication1

Organism-specific databases

DisGeNETi140691
OpenTargetsiENSG00000185880
PharmGKBiPA162407010

Polymorphism and mutation databases

BioMutaiTRIM69
DMDMi229462760

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782361 – 500E3 ubiquitin-protein ligase TRIM69Add BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei341PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is necessary for nuclear localization.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ86WT6
PeptideAtlasiQ86WT6
PRIDEiQ86WT6
TopDownProteomicsiQ86WT6-2 [Q86WT6-2]

PTM databases

iPTMnetiQ86WT6
PhosphoSitePlusiQ86WT6

Expressioni

Gene expression databases

BgeeiENSG00000185880
CleanExiHS_TRIM69
ExpressionAtlasiQ86WT6 baseline and differential
GenevisibleiQ86WT6 HS

Organism-specific databases

HPAiHPA028894

Interactioni

Subunit structurei

Interacts with PML.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi126654, 41 interactors
IntActiQ86WT6, 41 interactors
MINTiQ86WT6
STRINGi9606.ENSP00000332284

Structurei

Secondary structure

1500
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi144 – 148Combined sources5
Helixi153 – 260Combined sources108
Helixi266 – 271Combined sources6
Helixi273 – 287Combined sources15
Helixi298 – 301Combined sources4
Helixi305 – 318Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NQJX-ray2.15A/B/C143-321[»]
ProteinModelPortaliQ86WT6
SMRiQ86WT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini305 – 500B30.2/SPRYPROSITE-ProRule annotationAdd BLAST196

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 152Necessary for nuclear localizationBy similarityAdd BLAST152

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili161 – 255Sequence analysisAdd BLAST95

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localization and aggregation.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri41 – 82RING-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG410XWXM LUCA
GeneTreeiENSGT00760000118838
HOVERGENiHBG001357
InParanoidiQ86WT6
KOiK12034
OMAiFTSGKWY
OrthoDBiEOG091G05W2
PhylomeDBiQ86WT6
TreeFamiTF342569

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR006574 PRY
IPR003877 SPRY_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF13765 PRY, 1 hit
PF00622 SPRY, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00589 PRY, 1 hit
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86WT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSTNPSSN IDPGDYVEMN DSITHLPSKV VIQDITMELH CPLCNDWFRD
60 70 80 90 100
PLMLSCGHNF CEACIQDFWR LQAKETFCPE CKMLCQYNNC TFNPVLDKLV
110 120 130 140 150
EKIKKLPLLK GHPQCPEHGE NLKLFSKPDG KLICFQCKDA RLSVGQSKEF
160 170 180 190 200
LQISDAVHFF TEELAIQQGQ LETTLKELQT LRNMQKEAIA AHKENKLHLQ
210 220 230 240 250
QHVSMEFLKL HQFLHSKEKD ILTELREEGK ALNEEMELNL SQLQEQCLLA
260 270 280 290 300
KDMLVSIQAK TEQQNSFDFL KDITTLLHSL EQGMKVLATR ELISRKLNLG
310 320 330 340 350
QYKGPIQYMV WREMQDTLCP GLSPLTLDPK TAHPNLVLSK SQTSVWHGDI
360 370 380 390 400
KKIMPDDPER FDSSVAVLGS RGFTSGKWYW EVEVAKKTKW TVGVVRESII
410 420 430 440 450
RKGSCPLTPE QGFWLLRLRN QTDLKALDLP SFSLTLTNNL DKVGIYLDYE
460 470 480 490 500
GGQLSFYNAK TMTHIYTFSN TFMEKLYPYF CPCLNDGGEN KEPLHILHPQ
Length:500
Mass (Da):57,419
Last modified:May 5, 2009 - v2
Checksum:iE9957652895213B3
GO
Isoform 2 (identifier: Q86WT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     3-161: Missing.

Show »
Length:341
Mass (Da):39,254
Checksum:i36DF6708DB5DBF7B
GO
Isoform 3 (identifier: Q86WT6-3) [UniParc]FASTAAdd to basket
Also known as: Trimless alpha

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Show »
Length:296
Mass (Da):34,033
Checksum:iFD94B8F14B04945B
GO
Isoform 4 (identifier: Q86WT6-4) [UniParc]FASTAAdd to basket
Also known as: Trimless beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.
     255-271: Missing.

Show »
Length:279
Mass (Da):32,068
Checksum:i732E356FEF439FF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti469S → G in AAQ75551 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05722612D → N. Corresponds to variant dbSNP:rs2444007Ensembl.1
Natural variantiVAR_05722715D → N1 PublicationCorresponds to variant dbSNP:rs2470911Ensembl.1
Natural variantiVAR_03070431V → A. Corresponds to variant dbSNP:rs3759880Ensembl.1
Natural variantiVAR_057228104K → R. Corresponds to variant dbSNP:rs17588988Ensembl.1
Natural variantiVAR_057229161T → M1 PublicationCorresponds to variant dbSNP:rs3100139Ensembl.1
Natural variantiVAR_057230190A → V. Corresponds to variant dbSNP:rs3759880Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232001 – 204Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST204
Alternative sequenceiVSP_0232013 – 161Missing in isoform 2. 1 PublicationAdd BLAST159
Alternative sequenceiVSP_023202255 – 271Missing in isoform 4. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302088 mRNA Translation: AAL55810.1
AF302089 mRNA Translation: AAL55811.1
DQ232883 mRNA Translation: ABB18376.1
AY305385 mRNA Translation: AAQ75551.1
AC122108 Genomic DNA No translation available.
BC024199 mRNA Translation: AAH24199.1 Different termination.
BC033314 mRNA Translation: AAH33314.1
BC047945 mRNA Translation: AAH47945.1
CCDSiCCDS10114.1 [Q86WT6-2]
CCDS32220.1 [Q86WT6-1]
CCDS73719.1 [Q86WT6-3]
CCDS76746.1 [Q86WT6-4]
RefSeqiNP_001288073.1, NM_001301144.1 [Q86WT6-3]
NP_001288074.1, NM_001301145.1 [Q86WT6-4]
NP_001288075.1, NM_001301146.1
NP_542783.2, NM_080745.4 [Q86WT6-2]
NP_892030.3, NM_182985.4 [Q86WT6-1]
UniGeneiHs.745248

Genome annotation databases

EnsembliENST00000329464; ENSP00000332284; ENSG00000185880 [Q86WT6-1]
ENST00000338264; ENSP00000342922; ENSG00000185880 [Q86WT6-2]
ENST00000558329; ENSP00000453332; ENSG00000185880 [Q86WT6-4]
ENST00000559390; ENSP00000453177; ENSG00000185880 [Q86WT6-1]
ENST00000560442; ENSP00000453549; ENSG00000185880 [Q86WT6-3]
ENST00000618398; ENSP00000479720; ENSG00000278211 [Q86WT6-2]
ENST00000631740; ENSP00000488341; ENSG00000278211 [Q86WT6-4]
ENST00000633106; ENSP00000488084; ENSG00000278211 [Q86WT6-3]
GeneIDi140691
KEGGihsa:140691
UCSCiuc001zuf.3 human [Q86WT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTRI69_HUMAN
AccessioniPrimary (citable) accession number: Q86WT6
Secondary accession number(s): A8MX03
, Q309B0, Q4G1A5, Q6W897, Q8IYY3, Q8WY16, Q8WY17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: May 5, 2009
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health