Reviewed, UniProtKB/Swiss-Prot Q86WT6 (TRI69_HUMAN)

Names and origin

Protein names

Recommended name:
    Tripartite motif-containing protein 69
Alternative name(s):
    RING finger protein 36
    RFP-like domain-containing protein trimless

Gene names

Name:	TRIM69
Synonyms:	RNF36
ORF Names:	HSD34, HSD-34

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in apoptosis By similarity.
Subunit structure	Interacts with PML By similarity.
Subcellular location	Nucleus speckleBy similarity.
Post-translational modification	Phosphorylated. Phosphorylation is necessary for nuclear localization By similarity.
Sequence similarities	Belongs to the TRIM/RBCC family. Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86WT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86WT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3-161: Missing.

Isoform 3 (identifier: Q86WT6-3)

Also known as: Trimless alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Isoform 4 (identifier: Q86WT6-4)

Also known as: Trimless beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.
     255-271: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Tripartite motif-containing protein 69		PRO_0000278236
Regions
	Domain	305 – 500	196	B30.2/SPRY
	Zinc finger	41 – 82	42	RING-type
	Region	1 – 152	152	Necessary for nuclear localization By similarity
	Coiled coil	161 – 255	95	Potential
Natural variations
	Alternative sequence	1 – 204	204	Missing in isoform 3 and isoform 4.		VSP_023200
	Alternative sequence	3 – 161	159	Missing in isoform 2.		VSP_023201
	Alternative sequence	255 – 271	17	Missing in isoform 4.		VSP_023202
	Natural variant	31	1	V → A: dbSNP rs3759880.		VAR_030704
Experimental info
	Sequence conflict	15	1	N → D in ABB18376. Ref.2
	Sequence conflict	15	1	N → D in AAQ75551. Ref.3
	Sequence conflict	161	1	M → T in ABB18376. Ref.2
	Sequence conflict	469	1	S → G in AAQ75551. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0108A705BD03FDE1	FASTA	500	57,448
	10 20 30 40 50 60 MEVSTNPSSN IDPGNYVEMN DSITHLPSKV VIQDITMELH CPLCNDWFRD PLMLSCGHNF 70 80 90 100 110 120 CEACIQDFWR LQAKETFCPE CKMLCQYNNC TFNPVLDKLV EKIKKLPLLK GHPQCPEHGE 130 140 150 160 170 180 NLKLFSKPDG KLICFQCKDA RLSVGQSKEF LQISDAVHFF MEELAIQQGQ LETTLKELQT 190 200 210 220 230 240 LRNMQKEAIA AHKENKLHLQ QHVSMEFLKL HQFLHSKEKD ILTELREEGK ALNEEMELNL 250 260 270 280 290 300 SQLQEQCLLA KDMLVSIQAK TEQQNSFDFL KDITTLLHSL EQGMKVLATR ELISRKLNLG 310 320 330 340 350 360 QYKGPIQYMV WREMQDTLCP GLSPLTLDPK TAHPNLVLSK SQTSVWHGDI KKIMPDDPER 370 380 390 400 410 420 FDSSVAVLGS RGFTSGKWYW EVEVAKKTKW TVGVVRESII RKGSCPLTPE QGFWLLRLRN 430 440 450 460 470 480 QTDLKALDLP SFSLTLTNNL DKVGIYLDYE GGQLSFYNAK TMTHIYTFSN TFMEKLYPYF 490 500 CPCLNDGGEN KEPLHILHPQ
	Isoform 2 [UniParc]. Checksum: 36DF6708DB5DBF7B		341	39,254
	10 20 30 40 50 60 MEEELAIQQG QLETTLKELQ TLRNMQKEAI AAHKENKLHL QQHVSMEFLK LHQFLHSKEK 70 80 90 100 110 120 DILTELREEG KALNEEMELN LSQLQEQCLL AKDMLVSIQA KTEQQNSFDF LKDITTLLHS 130 140 150 160 170 180 LEQGMKVLAT RELISRKLNL GQYKGPIQYM VWREMQDTLC PGLSPLTLDP KTAHPNLVLS 190 200 210 220 230 240 KSQTSVWHGD IKKIMPDDPE RFDSSVAVLG SRGFTSGKWY WEVEVAKKTK WTVGVVRESI 250 260 270 280 290 300 IRKGSCPLTP EQGFWLLRLR NQTDLKALDL PSFSLTLTNN LDKVGIYLDY EGGQLSFYNA 310 320 330 340 KTMTHIYTFS NTFMEKLYPY FCPCLNDGGE NKEPLHILHP Q
	Isoform 3 (Trimless alpha) [UniParc]. Checksum: FD94B8F14B04945B		296	34,033
	10 20 30 40 50 60 MEFLKLHQFL HSKEKDILTE LREEGKALNE EMELNLSQLQ EQCLLAKDML VSIQAKTEQQ 70 80 90 100 110 120 NSFDFLKDIT TLLHSLEQGM KVLATRELIS RKLNLGQYKG PIQYMVWREM QDTLCPGLSP 130 140 150 160 170 180 LTLDPKTAHP NLVLSKSQTS VWHGDIKKIM PDDPERFDSS VAVLGSRGFT SGKWYWEVEV 190 200 210 220 230 240 AKKTKWTVGV VRESIIRKGS CPLTPEQGFW LLRLRNQTDL KALDLPSFSL TLTNNLDKVG 250 260 270 280 290 IYLDYEGGQL SFYNAKTMTH IYTFSNTFME KLYPYFCPCL NDGGENKEPL HILHPQ
	Isoform 4 (Trimless beta) [UniParc]. Checksum: 732E356FEF439FF9		279	32,068
	10 20 30 40 50 60 MEFLKLHQFL HSKEKDILTE LREEGKALNE EMELNLSQLQ EQCLLAKDML DITTLLHSLE 70 80 90 100 110 120 QGMKVLATRE LISRKLNLGQ YKGPIQYMVW REMQDTLCPG LSPLTLDPKT AHPNLVLSKS 130 140 150 160 170 180 QTSVWHGDIK KIMPDDPERF DSSVAVLGSR GFTSGKWYWE VEVAKKTKWT VGVVRESIIR 190 200 210 220 230 240 KGSCPLTPEQ GFWLLRLRNQ TDLKALDLPS FSLTLTNNLD KVGIYLDYEG GQLSFYNAKT 250 260 270 MTHIYTFSNT FMEKLYPYFC PCLNDGGENK EPLHILHPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TRIMLESS defines a new class of RFP-like domain containing proteins." Merla G., Zanaria E., Cairo S., Meroni G., Reymond A. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4).
[2]	Corcoran M., Lerner M., Sangfelt O., Grander D. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"A new spermatogenesis related gene." Fan M.M., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF302088 mRNA. Translation: AAL55810.1. AF302089 mRNA. Translation: AAL55811.1. DQ232883 mRNA. Translation: ABB18376.1. AY305385 mRNA. Translation: AAQ75551.1. BC024199 mRNA. Translation: AAH24199.1. Different termination. BC033314 mRNA. Translation: AAH33314.1. BC047945 mRNA. Translation: AAH47945.1.
RefSeq	NP_542783.2. NP_892030.3.
UniGene	Hs.489254
3D structure databases
HSSP	HSSP built from PDB template 1FRE based on UniProtKB Q92021.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86WT6.
Genome annotation databases
Ensembl	ENSG00000185880. Homo sapiens. [Contig view]
GeneID	140691.
KEGG	hsa:140691.
Organism-specific databases
HGNC	HGNC:17857. TRIM69.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q86WT6.
Gene expression databases
ArrayExpress	Q86WT6.
CleanEx	HS_TRIM69.
Family and domain databases
InterPro	IPR001870. B302. IPR003879. Butyrophylin. IPR006574. PRY. IPR003877. SPRY_rcpt. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF00622. SPRY. 1 hit. PF00097. zf-C3HC4. 1 hit. [Graphical view]
PRINTS	PR01407. BUTYPHLNCDUF.
SMART	SM00589. PRY. 1 hit. SM00184. RING. 1 hit. SM00449. SPRY. 1 hit. [Graphical view]
PROSITE	PS50188. B302_SPRY. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	84243.

Entry information

Entry name

TRI69_HUMAN

Accession

Primary (citable) accession number: Q86WT6
Secondary accession number(s): Q309B0 , Q4G1A5, Q6W897, Q8IYY3, Q8WY16, Q8WY17

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)