Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras association domain-containing protein 3

Gene

RASSF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. identical protein binding Source: IntAct

GO - Biological processi

  1. signal transduction Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ras association domain-containing protein 3
Gene namesi
Name:RASSF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:14271. RASSF3.

Subcellular locationi

  1. Cytoplasm 1 Publication
  2. Cytoplasmcytoskeleton 1 Publication

  3. Note: Localized to microtubules in vascular endothelial cells.

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. microtubule Source: UniProtKB-KW
  3. plasma membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34247.

Polymorphism and mutation databases

BioMutaiRASSF3.
DMDMi74714093.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 238237Ras association domain-containing protein 3PRO_0000240396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ86WH2.
PaxDbiQ86WH2.
PRIDEiQ86WH2.

PTM databases

PhosphoSiteiQ86WH2.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiQ86WH2.
CleanExiHS_RASSF3.
ExpressionAtlasiQ86WH2. baseline and differential.
GenevestigatoriQ86WH2.

Organism-specific databases

HPAiCAB034035.
HPA038469.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2845202,EBI-2845202
STK3Q131886EBI-2845202,EBI-992580
STK4Q130436EBI-2845202,EBI-367376

Protein-protein interaction databases

BioGridi129534. 7 interactions.
IntActiQ86WH2. 22 interactions.
STRINGi9606.ENSP00000336616.

Structurei

3D structure databases

ProteinModelPortaliQ86WH2.
SMRiQ86WH2. Positions 52-185, 189-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 186108Ras-associatingPROSITE-ProRule annotationAdd
BLAST
Domaini187 – 23448SARAHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 SARAH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG257702.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiQ86WH2.
KOiK09852.
OMAiKRCHKED.
OrthoDBiEOG7TF796.
PhylomeDBiQ86WH2.
TreeFamiTF319243.

Family and domain databases

InterProiIPR000159. Ras-assoc.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86WH2-1) [UniParc]FASTAAdd to basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSGYSSLEE DAEDFFFTAR TSFFRRAPQG KPRSGQQDVE KEKETHSYLS
60 70 80 90 100
KEEIKEKVHK YNLAVTDKLK MTLNSNGIYT GFIKVQMELC KPPQTSPNSG
110 120 130 140 150
KLSPSSNGCM NTLHISSTNT VGEVIEALLK KFLVTESPAK FALYKRCHRE
160 170 180 190 200
DQVYACKLSD REHPLYLRLV AGPRTDTLSF VLREHEIGEW EAFSLPELQN
210 220 230
FLRILDKEED EQLQNLKRRY TAYRQKLEEA LREVWKPD
Length:238
Mass (Da):27,562
Last modified:June 1, 2003 - v1
Checksum:i2C8AD10649259811
GO
Isoform 2 (identifier: Q86WH2-2) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     74-90: NSNGIYTGFIKVQMELC → STPASSQTGNIHSTCVW
     91-238: Missing.

Show »
Length:90
Mass (Da):10,265
Checksum:iEEFFEA9B843855E2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti232 – 2321R → H.1 Publication
Corresponds to variant rs74098110 [ dbSNP | Ensembl ].
VAR_026724

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei74 – 9017NSNGI…QMELC → STPASSQTGNIHSTCVW in isoform 2. 1 PublicationVSP_019353Add
BLAST
Alternative sequencei91 – 238148Missing in isoform 2. 1 PublicationVSP_019354Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY217662 mRNA. Translation: AAO61687.1.
AY217663 mRNA. Translation: AAO61688.1.
BC100951 mRNA. Translation: AAI00952.1.
BC100949 mRNA. Translation: AAI00950.1.
CCDSiCCDS8969.1. [Q86WH2-1]
RefSeqiNP_835463.1. NM_178169.3. [Q86WH2-1]
UniGeneiHs.643605.

Genome annotation databases

EnsembliENST00000283172; ENSP00000283172; ENSG00000153179. [Q86WH2-2]
ENST00000336061; ENSP00000336616; ENSG00000153179. [Q86WH2-1]
ENST00000542104; ENSP00000443021; ENSG00000153179. [Q86WH2-1]
GeneIDi283349.
KEGGihsa:283349.
UCSCiuc001ssd.3. human. [Q86WH2-1]

Polymorphism and mutation databases

BioMutaiRASSF3.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY217662 mRNA. Translation: AAO61687.1.
AY217663 mRNA. Translation: AAO61688.1.
BC100951 mRNA. Translation: AAI00952.1.
BC100949 mRNA. Translation: AAI00950.1.
CCDSiCCDS8969.1. [Q86WH2-1]
RefSeqiNP_835463.1. NM_178169.3. [Q86WH2-1]
UniGeneiHs.643605.

3D structure databases

ProteinModelPortaliQ86WH2.
SMRiQ86WH2. Positions 52-185, 189-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi129534. 7 interactions.
IntActiQ86WH2. 22 interactions.
STRINGi9606.ENSP00000336616.

PTM databases

PhosphoSiteiQ86WH2.

Polymorphism and mutation databases

BioMutaiRASSF3.
DMDMi74714093.

Proteomic databases

MaxQBiQ86WH2.
PaxDbiQ86WH2.
PRIDEiQ86WH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283172; ENSP00000283172; ENSG00000153179. [Q86WH2-2]
ENST00000336061; ENSP00000336616; ENSG00000153179. [Q86WH2-1]
ENST00000542104; ENSP00000443021; ENSG00000153179. [Q86WH2-1]
GeneIDi283349.
KEGGihsa:283349.
UCSCiuc001ssd.3. human. [Q86WH2-1]

Organism-specific databases

CTDi283349.
GeneCardsiGC12P065004.
HGNCiHGNC:14271. RASSF3.
HPAiCAB034035.
HPA038469.
MIMi607019. gene.
neXtProtiNX_Q86WH2.
PharmGKBiPA34247.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG257702.
GeneTreeiENSGT00390000003367.
HOGENOMiHOG000013025.
HOVERGENiHBG054362.
InParanoidiQ86WH2.
KOiK09852.
OMAiKRCHKED.
OrthoDBiEOG7TF796.
PhylomeDBiQ86WH2.
TreeFamiTF319243.

Miscellaneous databases

ChiTaRSiRASSF3. human.
GenomeRNAii283349.
NextBioi93829.
PROiQ86WH2.
SOURCEiSearch...

Gene expression databases

BgeeiQ86WH2.
CleanExiHS_RASSF3.
ExpressionAtlasiQ86WH2. baseline and differential.
GenevestigatoriQ86WH2.

Family and domain databases

InterProiIPR000159. Ras-assoc.
IPR011524. SARAH_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00314. RA. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50200. RA. 1 hit.
PS50951. SARAH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RASSF5 is transcriptionally inactivated by an epigenetic mechanism in ovarian cancers."
    Burbee D.G., White M.A., Miller D.S., Minna J.D., Muller C.Y.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. "RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1."
    Tommasi S., Dammann R., Jin S.-G., Zhang X.-F., Avruch J., Pfeifer G.P.
    Oncogene 21:2713-2720(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, VARIANT HIS-232.
  4. "Local activation of Rap1 contributes to directional vascular endothelial cell migration accompanied by extension of microtubules on which RAPL, a Rap1-associating molecule, localizes."
    Fujita H., Fukuhara S., Sakurai A., Yamagishi A., Kamioka Y., Nakaoka Y., Masuda M., Mochizuki N.
    J. Biol. Chem. 280:5022-5031(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRASF3_HUMAN
AccessioniPrimary (citable) accession number: Q86WH2
Secondary accession number(s): Q86WH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 1, 2003
Last modified: April 29, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was termed (Ref. 1) RASSF5.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.