Reviewed, UniProtKB/Swiss-Prot Q86W50 (MET10_HUMAN)

Names and origin

Protein names

Recommended name:
    Putative methyltransferase METT10D
    EC=2.1.1.-
Alternative name(s):
    Methyltransferase 10 domain-containing protein

Gene names

Name:

METT10D

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.
Sequence caution	The sequence BAB55094.1 differs from that shown. Reason: Erroneous termination at position 505. Translated as Lys. The sequence CAD89999.2 differs from that shown. Reason: Frameshift at position 424.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86W50-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86W50-2)

The sequence of this isoform differs from the canonical sequence as follows:
     214-224: GITEIMAEGGE → DSLEPGRWRLQ
     225-562: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 562

562

Putative methyltransferase METT10D

PRO_0000310767

Amino acid modifications

Modified residue

329

1

Phosphoserine

Modified residue

450

1

Phosphoserine By similarity

Natural variations

Alternative sequence

214 – 224

11

GITEIMAEGGE → DSLEPGRWRLQ in isoform 2.

VSP_029340

Alternative sequence

225 – 562

338

Missing in isoform 2.

VSP_029341

Natural variant

479

1

N → S: dbSNP rs17834783.

VAR_037086

Experimental info

Sequence conflict

39

1

N → D in BAB55094. Ref.2

Sequence conflict

214

1

G → A in CAD89999. Ref.1

Sequence conflict

419

1

S → R in BAB55094. Ref.2

Secondary structure

1

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562

Helix Strand Turn

Details...

Helix

49 – 64

16

Helix

79 – 93

15

Helix

98 – 100

3

Beta strand

105 – 110

6

Turn

112 – 115

4

Helix

116 – 125

10

Beta strand

128 – 134

7

Helix

136 – 148

13

Turn

152 – 154

3

Beta strand

155 – 159

5

Turn

167 – 169

3

Beta strand

178 – 183

6

Turn

215 – 218

4

Helix

219 – 222

4

Helix

224 – 238

15

Helix

239 – 241

3

Beta strand

245 – 251

7

Helix

255 – 264

10

Beta strand

268 – 277

10

Beta strand

280 – 289

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 003B55E4551F573F	FASTA	562	63,648
	10 20 30 40 50 60 MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL 70 80 90 100 110 120 REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL 130 140 150 160 170 180 GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF 190 200 210 220 230 240 CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK 250 260 270 280 290 300 RLRWYSCMLG KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS 310 320 330 340 350 360 KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL TDLKVQHKRV 370 380 390 400 410 420 PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE DVIQALEEKK PTPKESGNSQ 430 440 450 460 470 480 ELARGPQERT PCGPALREGE AAAVEGPCPS QESLSQEENP EPTEDERSEE KGGVEVLENC 490 500 510 520 530 540 QGSSNGAQDQ EASEQFGSPV AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN 550 560 RDLMNQLCTY IRNQIFRLVA VN
	Isoform 2 [UniParc]. Checksum: 15AB2DF8B5487A54		224	25,502
	10 20 30 40 50 60 MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL 70 80 90 100 110 120 REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL 130 140 150 160 170 180 GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF 190 200 210 220 CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGDSLEPGR WRLQ

References

[1]	The German cDNA consortium Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spinal cord.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT SER-479.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Muscle and Uterus.
[4]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AL832612 mRNA. Translation: CAD89999.2. Frameshift.
AK027410 mRNA. Translation: BAB55094.1. Sequence problems.
BC001213 mRNA. Translation: AAH01213.1.
BC050603 mRNA. Translation: AAH50603.1.

RefSeq

NP_076991.3.

UniGene

Hs.632237

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H00	X-ray	2.00	A/B/C	39-291	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q86W50.

Genome annotation databases

Ensembl

ENSG00000127804. Homo sapiens. [Contig view]

GeneID

79066.

KEGG

hsa:79066.

Organism-specific databases

HGNC

HGNC:28484. METT10D.

PharmGKB

PA142671460.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q86W50.

HOVERGEN

Q86W50.

Gene expression databases

ArrayExpress

Q86W50.

CleanEx

HS_METT10D.

Family and domain databases

InterPro

IPR017182. S-AdoMet-dep_MeTrfase_Mett10D.
IPR010286. SAM-Mtase_prd.
[Graphical view]

PANTHER

PTHR13393. DUF890. 1 hit.

Pfam

PF05971. Methyltransf_10. 1 hit.
[Graphical view]

PIRSF

PIRSF037350. Mtase_ZK1128_prd. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

67841.

Entry information

Entry name

MET10_HUMAN

Accession

Primary (citable) accession number: Q86W50
Secondary accession number(s): Q86TE5, Q96T16, Q9BVG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)