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Protein

Methyltransferase-like protein 16

Gene

METTL16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable methyltransferase.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821S-adenosyl-L-methionineCombined sources
Binding sitei110 – 1101S-adenosyl-L-methionine; via carbonyl oxygenCombined sources
Binding sitei133 – 1331S-adenosyl-L-methionineCombined sources
Binding sitei164 – 1641S-adenosyl-L-methionineCombined sources
Binding sitei184 – 1841S-adenosyl-L-methionineCombined sources

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Methyltransferase-like protein 16 (EC:2.1.1.-)
Alternative name(s):
Methyltransferase 10 domain-containing protein
Gene namesi
Name:METTL16
Synonyms:METT10D
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:28484. METTL16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671460.

Polymorphism and mutation databases

BioMutaiMETTL16.
DMDMi269849619.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 562562Methyltransferase-like protein 16PRO_0000310767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei329 – 3291PhosphoserineCombined sources
Modified residuei463 – 4631PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86W50.
MaxQBiQ86W50.
PaxDbiQ86W50.
PeptideAtlasiQ86W50.
PRIDEiQ86W50.

PTM databases

iPTMnetiQ86W50.
PhosphoSiteiQ86W50.

Expressioni

Gene expression databases

BgeeiQ86W50.
CleanExiHS_METT10D.
ExpressionAtlasiQ86W50. baseline and differential.
GenevisibleiQ86W50. HS.

Organism-specific databases

HPAiHPA020352.
HPA059798.

Interactioni

Protein-protein interaction databases

BioGridi122519. 9 interactions.
STRINGi9606.ENSP00000263092.

Structurei

Secondary structure

1
562
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi49 – 6416Combined sources
Helixi79 – 9315Combined sources
Helixi98 – 1003Combined sources
Beta strandi105 – 1106Combined sources
Turni112 – 1154Combined sources
Helixi116 – 12510Combined sources
Beta strandi128 – 1347Combined sources
Helixi136 – 14813Combined sources
Turni152 – 1543Combined sources
Beta strandi155 – 1595Combined sources
Turni167 – 1693Combined sources
Beta strandi178 – 1836Combined sources
Turni215 – 2184Combined sources
Helixi219 – 2224Combined sources
Helixi224 – 23815Combined sources
Helixi239 – 2413Combined sources
Beta strandi245 – 2517Combined sources
Helixi255 – 26410Combined sources
Beta strandi268 – 27710Combined sources
Beta strandi280 – 28910Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H00X-ray2.00A/B/C40-291[»]
ProteinModelPortaliQ86W50.
SMRiQ86W50. Positions 42-291.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86W50.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2912. Eukaryota.
COG3129. LUCA.
GeneTreeiENSGT00390000016694.
HOGENOMiHOG000181023.
HOVERGENiHBG058052.
InParanoidiQ86W50.
KOiK11393.
OMAiESCKGSS.
OrthoDBiEOG7CCBRH.
PhylomeDBiQ86W50.
TreeFamiTF313132.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR010286. rRNA_lsu_MeTfrase_F-like.
IPR017182. S-AdoMet-dep_MeTrfase_Mett10D.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF037350. Mtase_ZK1128_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86W50-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE
60 70 80 90 100
AVRALTCTLL REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK
110 120 130 140 150
STLRRGIDIG TGASCIYPLL GATLNGWYFL ATEVDDMCFN YAKKNVEQNN
160 170 180 190 200
LSDLIKVVKV PQKTLLMDAL KEESEIIYDF CMCNPPFFAN QLEAKGVNSR
210 220 230 240 250
NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK RLRWYSCMLG
260 270 280 290 300
KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS
310 320 330 340 350
KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL
360 370 380 390 400
TDLKVQHKRV PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE
410 420 430 440 450
DVIQALEEKK PTPKESGNSQ ELARGPQERT PCGPALREGE AAAVEGPCPS
460 470 480 490 500
QESLSQEENP EPTEDERSEE KGGVEVLESC QGSSNGAQDQ EASEQFGSPV
510 520 530 540 550
AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN RDLMNQLCTY
560
IRNQIFRLVA VN
Length:562
Mass (Da):63,621
Last modified:November 24, 2009 - v2
Checksum:i8CF655F19AF572F0
GO
Isoform 2 (identifier: Q86W50-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     214-224: GITEIMAEGGE → DSLEPGRWRLQ
     225-562: Missing.

Note: No experimental confirmation available.
Show »
Length:224
Mass (Da):25,502
Checksum:i15AB2DF8B5487A54
GO

Sequence cautioni

The sequence BAB55094.1 differs from that shown. Reason: Erroneous termination at position 505. Translated as Lys.Curated
The sequence CAD89999.2 differs from that shown. Reason: Frameshift at position 424. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti39 – 391N → D in BAB55094 (PubMed:14702039).Curated
Sequence conflicti214 – 2141G → A in CAD89999 (PubMed:17974005).Curated
Sequence conflicti419 – 4191S → R in BAB55094 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti479 – 4791S → N.1 Publication
Corresponds to variant rs17834783 [ dbSNP | Ensembl ].
VAR_037086

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei214 – 22411GITEIMAEGGE → DSLEPGRWRLQ in isoform 2. 1 PublicationVSP_029340Add
BLAST
Alternative sequencei225 – 562338Missing in isoform 2. 1 PublicationVSP_029341Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027410 mRNA. Translation: BAB55094.1. Sequence problems.
AL832612 mRNA. Translation: CAD89999.2. Frameshift.
AC006435 Genomic DNA. No translation available.
AC015799 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90537.1.
CH471108 Genomic DNA. Translation: EAW90538.1.
BC001213 mRNA. Translation: AAH01213.1.
BC050603 mRNA. Translation: AAH50603.1.
CCDSiCCDS42232.1. [Q86W50-1]
RefSeqiNP_076991.3. NM_024086.3. [Q86W50-1]
UniGeneiHs.632237.

Genome annotation databases

EnsembliENST00000263092; ENSP00000263092; ENSG00000127804. [Q86W50-1]
GeneIDi79066.
KEGGihsa:79066.
UCSCiuc002fut.4. human. [Q86W50-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027410 mRNA. Translation: BAB55094.1. Sequence problems.
AL832612 mRNA. Translation: CAD89999.2. Frameshift.
AC006435 Genomic DNA. No translation available.
AC015799 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90537.1.
CH471108 Genomic DNA. Translation: EAW90538.1.
BC001213 mRNA. Translation: AAH01213.1.
BC050603 mRNA. Translation: AAH50603.1.
CCDSiCCDS42232.1. [Q86W50-1]
RefSeqiNP_076991.3. NM_024086.3. [Q86W50-1]
UniGeneiHs.632237.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2H00X-ray2.00A/B/C40-291[»]
ProteinModelPortaliQ86W50.
SMRiQ86W50. Positions 42-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122519. 9 interactions.
STRINGi9606.ENSP00000263092.

PTM databases

iPTMnetiQ86W50.
PhosphoSiteiQ86W50.

Polymorphism and mutation databases

BioMutaiMETTL16.
DMDMi269849619.

Proteomic databases

EPDiQ86W50.
MaxQBiQ86W50.
PaxDbiQ86W50.
PeptideAtlasiQ86W50.
PRIDEiQ86W50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263092; ENSP00000263092; ENSG00000127804. [Q86W50-1]
GeneIDi79066.
KEGGihsa:79066.
UCSCiuc002fut.4. human. [Q86W50-1]

Organism-specific databases

CTDi79066.
GeneCardsiMETTL16.
H-InvDBHIX0013421.
HGNCiHGNC:28484. METTL16.
HPAiHPA020352.
HPA059798.
neXtProtiNX_Q86W50.
PharmGKBiPA142671460.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2912. Eukaryota.
COG3129. LUCA.
GeneTreeiENSGT00390000016694.
HOGENOMiHOG000181023.
HOVERGENiHBG058052.
InParanoidiQ86W50.
KOiK11393.
OMAiESCKGSS.
OrthoDBiEOG7CCBRH.
PhylomeDBiQ86W50.
TreeFamiTF313132.

Miscellaneous databases

ChiTaRSiMETTL16. human.
EvolutionaryTraceiQ86W50.
GenomeRNAii79066.
PROiQ86W50.

Gene expression databases

BgeeiQ86W50.
CleanExiHS_METT10D.
ExpressionAtlasiQ86W50. baseline and differential.
GenevisibleiQ86W50. HS.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR010286. rRNA_lsu_MeTfrase_F-like.
IPR017182. S-AdoMet-dep_MeTrfase_Mett10D.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF037350. Mtase_ZK1128_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Spinal cord.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASN-479.
    Tissue: Muscle and Uterus.
  6. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.
  10. "The crystal structure of human methyltransferase 10 domain-containing protein."
    Structural genomics consortium (SGC)
    Submitted (JUN-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 39-291 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.

Entry informationi

Entry nameiMET16_HUMAN
AccessioniPrimary (citable) accession number: Q86W50
Secondary accession number(s): D3DTI8
, Q86TE5, Q96T16, Q9BVG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 24, 2009
Last modified: July 6, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
  6. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.