Q86W50 (MET16_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyltransferase-like protein 16
EC=2.1.1.-

Alternative name(s):
Methyltransferase 10 domain-containing protein

Gene names

Name:	METTL16
Synonyms:	METT10D

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable methyltransferase By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity. Ref.6 Ref.7
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.
Sequence caution	The sequence BAB55094.1 differs from that shown. Reason: Erroneous termination at position 505. Translated as Lys. The sequence CAD89999.2 differs from that shown. Reason: Frameshift at position 424.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86W50-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86W50-2)

The sequence of this isoform differs from the canonical sequence as follows:
     214-224: GITEIMAEGGE → DSLEPGRWRLQ
     225-562: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 562

562

Methyltransferase-like protein 16

PRO_0000310767

Sites

Binding site

82

1

S-adenosyl-L-methionine

Binding site

110

1

S-adenosyl-L-methionine; via carbonyl oxygen

Binding site

133

1

S-adenosyl-L-methionine

Binding site

164

1

S-adenosyl-L-methionine

Binding site

184

1

S-adenosyl-L-methionine

Amino acid modifications

Modified residue

329

1

Phosphoserine Ref.6 Ref.7

Modified residue

450

1

Phosphoserine By similarity

Natural variations

Alternative sequence

214 – 224

11

GITEIMAEGGE → DSLEPGRWRLQ in isoform 2.

VSP_029340

Alternative sequence

225 – 562

338

Missing in isoform 2.

VSP_029341

Natural variant

479

1

S → N. Ref.5
Corresponds to variant rs17834783 [ dbSNP | Ensembl ].

VAR_037086

Experimental info

Sequence conflict

39

1

N → D in BAB55094. Ref.1

Sequence conflict

214

1

G → A in CAD89999. Ref.2

Sequence conflict

419

1

S → R in BAB55094. Ref.1

Secondary structure

1

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562

Helix Strand Turn

Details...

Helix

49 – 64

16

Helix

79 – 93

15

Helix

98 – 100

3

Beta strand

105 – 110

6

Turn

112 – 115

4

Helix

116 – 125

10

Beta strand

128 – 134

7

Helix

136 – 148

13

Turn

152 – 154

3

Beta strand

155 – 159

5

Turn

167 – 169

3

Beta strand

178 – 183

6

Turn

215 – 218

4

Helix

219 – 222

4

Helix

224 – 238

15

Helix

239 – 241

3

Beta strand

245 – 251

7

Helix

255 – 264

10

Beta strand

268 – 277

10

Beta strand

280 – 289

10

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: 8CF655F19AF572F0	FASTA	562	63,621
	10 20 30 40 50 60 MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL 70 80 90 100 110 120 REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL 130 140 150 160 170 180 GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF 190 200 210 220 230 240 CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK 250 260 270 280 290 300 RLRWYSCMLG KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS 310 320 330 340 350 360 KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL TDLKVQHKRV 370 380 390 400 410 420 PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE DVIQALEEKK PTPKESGNSQ 430 440 450 460 470 480 ELARGPQERT PCGPALREGE AAAVEGPCPS QESLSQEENP EPTEDERSEE KGGVEVLESC 490 500 510 520 530 540 QGSSNGAQDQ EASEQFGSPV AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN 550 560 RDLMNQLCTY IRNQIFRLVA VN
	Isoform 2 [UniParc]. Checksum: 15AB2DF8B5487A54	FASTA	224	25,502
	10 20 30 40 50 60 MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL 70 80 90 100 110 120 REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL 130 140 150 160 170 180 GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF 190 200 210 220 CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGDSLEPGR WRLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spinal cord.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ASN-479. Tissue: Muscle and Uterus.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"The crystal structure of human methyltransferase 10 domain-containing protein." Structural genomics consortium (SGC) Submitted (JUN-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 39-291 IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK027410 mRNA. Translation: BAB55094.1. Sequence problems.
AL832612 mRNA. Translation: CAD89999.2. Frameshift.
AC006435 Genomic DNA. No translation available.
AC015799 Genomic DNA. No translation available.
CH471108 Genomic DNA. Translation: EAW90537.1.
CH471108 Genomic DNA. Translation: EAW90538.1.
BC001213 mRNA. Translation: AAH01213.1.
BC050603 mRNA. Translation: AAH50603.1.

IPI

IPI00163391.
IPI00792378.

RefSeq

NP_076991.3. NM_024086.3.

UniGene

Hs.632237.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H00	X-ray	2.00	A/B/C	40-291	[»]

ProteinModelPortal

Q86W50.

SMR

Q86W50. Positions 42-291.

ModBase

Search...

Protein-protein interaction databases

STRING

Q86W50.

PTM databases

PhosphoSite

Q86W50.

Polymorphism databases

DMDM

269849619.

Proteomic databases

PRIDE

Q86W50.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000263092; ENSP00000263092; ENSG00000127804.

GeneID

79066.

KEGG

hsa:79066.

UCSC

uc002fut.1. human.
uc002fuv.2. human.

Organism-specific databases

CTD

79066.

GeneCards

GC17M002319.

HGNC

HGNC:28484. METTL16.

HPA

HPA020352.

neXtProt

NX_Q86W50.

PharmGKB

PA142671460.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG15757.

GeneTree

ENSGT00390000016694.

HOGENOM

HBG281202.

HOVERGEN

HBG058052.

InParanoid

Q86W50.

OMA

CTYLRNQ.

OrthoDB

EOG4N5VWJ.

PhylomeDB

Q86W50.

Gene expression databases

ArrayExpress

Q86W50.

Bgee

Q86W50.

CleanEx

HS_METT10D.

Genevestigator

Q86W50.

Family and domain databases

InterPro

IPR017182. S-AdoMet-dep_MeTrfase_Mett10D.
IPR010286. SAM-Mtase_prd.
[Graphical view]

KO

K11393.

PANTHER

PTHR13393. DUF890. 1 hit.

Pfam

PF05971. Methyltransf_10. 1 hit.
[Graphical view]

PIRSF

PIRSF037350. Mtase_ZK1128_prd. 1 hit.

ProtoNet

Search...

Other

NextBio

67841.

Entry information

Entry name

MET16_HUMAN

Accession

Primary (citable) accession number: Q86W50
Secondary accession number(s): D3DTI8 , Q86TE5, Q96T16, Q9BVG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.