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Q86W34

- AMZ2_HUMAN

UniProt

Q86W34 - AMZ2_HUMAN

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Protein

Archaemetzincin-2

Gene
AMZ2, BM-014
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze either neurogranin or angiotensin-2.1 Publication

Cofactori

Binds 2 zinc ions per subunit. One is catalytic, whereas the other seems to have a structural role By similarity.

Enzyme regulationi

Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541Zinc 1; catalytic; via tele nitrogen By similarity
Active sitei255 – 2551Proton acceptor By similarity
Metal bindingi258 – 2581Zinc 1; catalytic; via tele nitrogen By similarity
Metal bindingi264 – 2641Zinc 1; catalytic; via tele nitrogen By similarity
Metal bindingi265 – 2651Zinc 2 By similarity
Metal bindingi270 – 2701Zinc 2 By similarity
Metal bindingi289 – 2891Zinc 2 By similarity
Metal bindingi292 – 2921Zinc 2 By similarity

GO - Molecular functioni

  1. metallopeptidase activity Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM54.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaemetzincin-2 (EC:3.4.-.-)
Alternative name(s):
Archeobacterial metalloproteinase-like protein 2
Gene namesi
Name:AMZ2
ORF Names:BM-014
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:28041. AMZ2.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162376414.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Archaemetzincin-2PRO_0000159617Add
BLAST

Proteomic databases

MaxQBiQ86W34.
PaxDbiQ86W34.
PRIDEiQ86W34.

PTM databases

PhosphoSiteiQ86W34.

Expressioni

Tissue specificityi

Predominantly expressed in heart and testis. Also expressed at lower levels in kidney, liver, pancreas, lung, brain and placenta. Expressed in fetal tissues such as kidney, liver, lung and brain. Down-regulated in testis from patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS).2 Publications

Gene expression databases

ArrayExpressiQ86W34.
BgeeiQ86W34.
CleanExiHS_AMZ2.
GenevestigatoriQ86W34.

Organism-specific databases

HPAiHPA023299.
HPA052406.

Interactioni

Protein-protein interaction databases

BioGridi119472. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ86W34.
SMRiQ86W34. Positions 144-297.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M54 family.

Phylogenomic databases

eggNOGiCOG1913.
HOGENOMiHOG000293350.
HOVERGENiHBG080165.
InParanoidiQ86W34.
KOiK06974.
OMAiKLQCAIG.
OrthoDBiEOG7WX08V.
PhylomeDBiQ86W34.
TreeFamiTF328603.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view]
PfamiPF07998. Peptidase_M54. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q86W34-4) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQIIRHSEQT LKTALISKNP VLVSQYEKLN AGEQRLMNEA FQPASDLFGP    50
ITLHSPSDWI TSHPEAPQDF EQFFSDPYRK TPSPNKRSIY IQSIGSLGNT 100
RIISEEYIKW LTGYCKAYFY GLRVKLLEPV PVSVTRCSFR VNENTHNLQI 150
HAGDILKFLK KKKPEDAFCV VGITMIDLYP RDSWNFVFGQ ASLTDGVGIF 200
SFARYGSDFY SMHYKGKVKK LKKTSSSDYS IFDNYYIPEI TSVLLLRSCK 250
TLTHEIGHIF GLRHCQWLAC LMQGSNHLEE ADRRPLNLCP ICLHKLQCAV 300
GFSIVERYKA LVRWIDDESS DTPGATPEHS HEDNGNLPKP VEAFKEWKEW 350
IIKCLAVLQK 360
Length:360
Mass (Da):41,263
Last modified:January 11, 2011 - v2
Checksum:iD74E7F7CE7873CBF
GO
Isoform 2 (identifier: Q86W34-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-153: Missing.

Note: Gene prediction based on EST data.

Show »
Length:302
Mass (Da):34,595
Checksum:i2AE9FB8B77CC8293
GO

Sequence cautioni

The sequence AAF64270.1 differs from that shown. Reason: Frameshift at position 55.
The sequence AAF64270.1 differs from that shown. Reason: Erroneous termination at position 98. Translated as Gly.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301N → D.5 Publications
Corresponds to variant rs3213690 [ dbSNP | Ensembl ].
VAR_024850
Natural varianti146 – 1461H → Q.
Corresponds to variant rs3207194 [ dbSNP | Ensembl ].
VAR_047343

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei96 – 15358Missing in isoform 2. VSP_047263Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41I → V in AAF64270. 1 Publication
Sequence conflicti6 – 61H → Y in AAF64270. 1 Publication
Sequence conflicti13 – 131T → I in AAF64270. 1 Publication
Sequence conflicti15 – 151L → V in AAF64270. 1 Publication
Sequence conflicti29 – 291L → V in AAF64270. 1 Publication
Sequence conflicti51 – 522IT → CI in AAF64270. 1 Publication
Sequence conflicti76 – 761D → H in AAF64270. 1 Publication
Sequence conflicti81 – 811T → I in AAF64270. 1 Publication
Sequence conflicti85 – 851N → D in AAF64270. 1 Publication
Sequence conflicti92 – 921Q → R in AAF64270. 1 Publication
Sequence conflicti99 – 991N → S in AAF64270. 1 Publication
Sequence conflicti121 – 1211G → R in AAF64270. 1 Publication
Sequence conflicti207 – 2071S → G in AAF64270. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ635358 mRNA. Translation: CAG25750.1.
AF208856 mRNA. Translation: AAF64270.1. Sequence problems.
AK126146 mRNA. Translation: BAC86462.1.
AK090981 mRNA. Translation: BAG52256.1.
AC005332 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89050.1.
BC050709 mRNA. Translation: AAH50709.1.
BC056271 mRNA. Translation: AAH56271.1.
CCDSiCCDS11674.1. [Q86W34-4]
CCDS32714.1. [Q86W34-5]
RefSeqiNP_001028741.1. NM_001033569.1. [Q86W34-4]
NP_001028742.1. NM_001033570.1. [Q86W34-4]
NP_001028743.1. NM_001033571.1. [Q86W34-4]
NP_001028744.1. NM_001033572.1. [Q86W34-4]
NP_001028746.1. NM_001033574.1. [Q86W34-5]
NP_001275983.1. NM_001289054.1. [Q86W34-4]
NP_001275985.1. NM_001289056.1. [Q86W34-4]
NP_057711.3. NM_016627.4. [Q86W34-4]
XP_005257490.1. XM_005257433.2. [Q86W34-4]
XP_005257492.1. XM_005257435.2. [Q86W34-4]
XP_005257493.1. XM_005257436.1. [Q86W34-4]
XP_005257494.1. XM_005257437.1. [Q86W34-4]
UniGeneiHs.293560.

Genome annotation databases

EnsembliENST00000359783; ENSP00000352831; ENSG00000196704. [Q86W34-5]
ENST00000359904; ENSP00000352976; ENSG00000196704. [Q86W34-4]
ENST00000392720; ENSP00000376481; ENSG00000196704. [Q86W34-4]
ENST00000573646; ENSP00000458752; ENSG00000262747. [Q86W34-4]
ENST00000574972; ENSP00000458372; ENSG00000262747. [Q86W34-5]
ENST00000575682; ENSP00000461778; ENSG00000262747. [Q86W34-4]
ENST00000577866; ENSP00000464133; ENSG00000196704. [Q86W34-4]
ENST00000577985; ENSP00000464635; ENSG00000196704. [Q86W34-4]
ENST00000580753; ENSP00000463012; ENSG00000196704. [Q86W34-4]
ENST00000582462; ENSP00000463297; ENSG00000262747. [Q86W34-4]
ENST00000583572; ENSP00000462911; ENSG00000262747. [Q86W34-4]
ENST00000584964; ENSP00000462793; ENSG00000262747. [Q86W34-4]
GeneIDi51321.
KEGGihsa:51321.
UCSCiuc002jgr.1. human. [Q86W34-4]

Polymorphism databases

DMDMi317373321.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ635358 mRNA. Translation: CAG25750.1 .
AF208856 mRNA. Translation: AAF64270.1 . Sequence problems.
AK126146 mRNA. Translation: BAC86462.1 .
AK090981 mRNA. Translation: BAG52256.1 .
AC005332 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89050.1 .
BC050709 mRNA. Translation: AAH50709.1 .
BC056271 mRNA. Translation: AAH56271.1 .
CCDSi CCDS11674.1. [Q86W34-4 ]
CCDS32714.1. [Q86W34-5 ]
RefSeqi NP_001028741.1. NM_001033569.1. [Q86W34-4 ]
NP_001028742.1. NM_001033570.1. [Q86W34-4 ]
NP_001028743.1. NM_001033571.1. [Q86W34-4 ]
NP_001028744.1. NM_001033572.1. [Q86W34-4 ]
NP_001028746.1. NM_001033574.1. [Q86W34-5 ]
NP_001275983.1. NM_001289054.1. [Q86W34-4 ]
NP_001275985.1. NM_001289056.1. [Q86W34-4 ]
NP_057711.3. NM_016627.4. [Q86W34-4 ]
XP_005257490.1. XM_005257433.2. [Q86W34-4 ]
XP_005257492.1. XM_005257435.2. [Q86W34-4 ]
XP_005257493.1. XM_005257436.1. [Q86W34-4 ]
XP_005257494.1. XM_005257437.1. [Q86W34-4 ]
UniGenei Hs.293560.

3D structure databases

ProteinModelPortali Q86W34.
SMRi Q86W34. Positions 144-297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119472. 2 interactions.

Protein family/group databases

MEROPSi M54.002.

PTM databases

PhosphoSitei Q86W34.

Polymorphism databases

DMDMi 317373321.

Proteomic databases

MaxQBi Q86W34.
PaxDbi Q86W34.
PRIDEi Q86W34.

Protocols and materials databases

DNASUi 51321.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000359783 ; ENSP00000352831 ; ENSG00000196704 . [Q86W34-5 ]
ENST00000359904 ; ENSP00000352976 ; ENSG00000196704 . [Q86W34-4 ]
ENST00000392720 ; ENSP00000376481 ; ENSG00000196704 . [Q86W34-4 ]
ENST00000573646 ; ENSP00000458752 ; ENSG00000262747 . [Q86W34-4 ]
ENST00000574972 ; ENSP00000458372 ; ENSG00000262747 . [Q86W34-5 ]
ENST00000575682 ; ENSP00000461778 ; ENSG00000262747 . [Q86W34-4 ]
ENST00000577866 ; ENSP00000464133 ; ENSG00000196704 . [Q86W34-4 ]
ENST00000577985 ; ENSP00000464635 ; ENSG00000196704 . [Q86W34-4 ]
ENST00000580753 ; ENSP00000463012 ; ENSG00000196704 . [Q86W34-4 ]
ENST00000582462 ; ENSP00000463297 ; ENSG00000262747 . [Q86W34-4 ]
ENST00000583572 ; ENSP00000462911 ; ENSG00000262747 . [Q86W34-4 ]
ENST00000584964 ; ENSP00000462793 ; ENSG00000262747 . [Q86W34-4 ]
GeneIDi 51321.
KEGGi hsa:51321.
UCSCi uc002jgr.1. human. [Q86W34-4 ]

Organism-specific databases

CTDi 51321.
GeneCardsi GC17P066244.
HGNCi HGNC:28041. AMZ2.
HPAi HPA023299.
HPA052406.
MIMi 615169. gene.
neXtProti NX_Q86W34.
PharmGKBi PA162376414.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1913.
HOGENOMi HOG000293350.
HOVERGENi HBG080165.
InParanoidi Q86W34.
KOi K06974.
OMAi KLQCAIG.
OrthoDBi EOG7WX08V.
PhylomeDBi Q86W34.
TreeFami TF328603.

Miscellaneous databases

ChiTaRSi AMZ2. human.
GenomeRNAii 51321.
NextBioi 54701.
PROi Q86W34.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q86W34.
Bgeei Q86W34.
CleanExi HS_AMZ2.
Genevestigatori Q86W34.

Family and domain databases

Gene3Di 3.40.390.10. 1 hit.
InterProi IPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view ]
Pfami PF07998. Peptidase_M54. 1 hit.
[Graphical view ]
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea."
    Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C.
    J. Biol. Chem. 280:30367-30375(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, VARIANT ASP-30.
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Umbilical cord blood.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Amygdala and Thymus.
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-30.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Bone marrow and Lung.
  7. "Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue."
    Lin Y.H., Lin Y.M., Teng Y.N., Hsieh T.Y., Lin Y.S., Kuo P.L.
    Fertil. Steril. 86:1650-1658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAMZ2_HUMAN
AccessioniPrimary (citable) accession number: Q86W34
Secondary accession number(s): A6NLD9
, B3KR44, Q5XKF1, Q9NZE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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