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Q86W34

- AMZ2_HUMAN

UniProt

Q86W34 - AMZ2_HUMAN

Protein

Archaemetzincin-2

Gene

AMZ2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze either neurogranin or angiotensin-2.1 Publication

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic, whereas the other seems to have a structural role By similarity.By similarity

    Enzyme regulationi

    Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively.1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi254 – 2541Zinc 1; catalytic; via tele nitrogenBy similarity
    Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
    Metal bindingi258 – 2581Zinc 1; catalytic; via tele nitrogenBy similarity
    Metal bindingi264 – 2641Zinc 1; catalytic; via tele nitrogenBy similarity
    Metal bindingi265 – 2651Zinc 2By similarity
    Metal bindingi270 – 2701Zinc 2By similarity
    Metal bindingi289 – 2891Zinc 2By similarity
    Metal bindingi292 – 2921Zinc 2By similarity

    GO - Molecular functioni

    1. metallopeptidase activity Source: UniProtKB-KW
    2. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM54.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Archaemetzincin-2 (EC:3.4.-.-)
    Alternative name(s):
    Archeobacterial metalloproteinase-like protein 2
    Gene namesi
    Name:AMZ2
    ORF Names:BM-014
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:28041. AMZ2.

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162376414.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360Archaemetzincin-2PRO_0000159617Add
    BLAST

    Proteomic databases

    MaxQBiQ86W34.
    PaxDbiQ86W34.
    PRIDEiQ86W34.

    PTM databases

    PhosphoSiteiQ86W34.

    Expressioni

    Tissue specificityi

    Predominantly expressed in heart and testis. Also expressed at lower levels in kidney, liver, pancreas, lung, brain and placenta. Expressed in fetal tissues such as kidney, liver, lung and brain. Down-regulated in testis from patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS).2 Publications

    Gene expression databases

    ArrayExpressiQ86W34.
    BgeeiQ86W34.
    CleanExiHS_AMZ2.
    GenevestigatoriQ86W34.

    Organism-specific databases

    HPAiHPA023299.
    HPA052406.

    Interactioni

    Protein-protein interaction databases

    BioGridi119472. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86W34.
    SMRiQ86W34. Positions 144-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M54 family.Curated

    Phylogenomic databases

    eggNOGiCOG1913.
    HOGENOMiHOG000293350.
    HOVERGENiHBG080165.
    InParanoidiQ86W34.
    KOiK06974.
    OMAiKLQCAIG.
    OrthoDBiEOG7WX08V.
    PhylomeDBiQ86W34.
    TreeFamiTF328603.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR012962. Pept_M54_archaemetzincn.
    [Graphical view]
    PfamiPF07998. Peptidase_M54. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86W34-4) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MQIIRHSEQT LKTALISKNP VLVSQYEKLN AGEQRLMNEA FQPASDLFGP    50
    ITLHSPSDWI TSHPEAPQDF EQFFSDPYRK TPSPNKRSIY IQSIGSLGNT 100
    RIISEEYIKW LTGYCKAYFY GLRVKLLEPV PVSVTRCSFR VNENTHNLQI 150
    HAGDILKFLK KKKPEDAFCV VGITMIDLYP RDSWNFVFGQ ASLTDGVGIF 200
    SFARYGSDFY SMHYKGKVKK LKKTSSSDYS IFDNYYIPEI TSVLLLRSCK 250
    TLTHEIGHIF GLRHCQWLAC LMQGSNHLEE ADRRPLNLCP ICLHKLQCAV 300
    GFSIVERYKA LVRWIDDESS DTPGATPEHS HEDNGNLPKP VEAFKEWKEW 350
    IIKCLAVLQK 360
    Length:360
    Mass (Da):41,263
    Last modified:January 11, 2011 - v2
    Checksum:iD74E7F7CE7873CBF
    GO
    Isoform 2 (identifier: Q86W34-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         96-153: Missing.

    Note: Gene prediction based on EST data.

    Show »
    Length:302
    Mass (Da):34,595
    Checksum:i2AE9FB8B77CC8293
    GO

    Sequence cautioni

    The sequence AAF64270.1 differs from that shown. Reason: Frameshift at position 55.
    The sequence AAF64270.1 differs from that shown. Reason: Erroneous termination at position 98. Translated as Gly.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41I → V in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti6 – 61H → Y in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti13 – 131T → I in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti15 – 151L → V in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti29 – 291L → V in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti51 – 522IT → CI in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti76 – 761D → H in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti81 – 811T → I in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti85 – 851N → D in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti92 – 921Q → R in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti99 – 991N → S in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti121 – 1211G → R in AAF64270. (PubMed:11042152)Curated
    Sequence conflicti207 – 2071S → G in AAF64270. (PubMed:11042152)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti30 – 301N → D.5 Publications
    Corresponds to variant rs3213690 [ dbSNP | Ensembl ].
    VAR_024850
    Natural varianti146 – 1461H → Q.
    Corresponds to variant rs3207194 [ dbSNP | Ensembl ].
    VAR_047343

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei96 – 15358Missing in isoform 2. CuratedVSP_047263Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ635358 mRNA. Translation: CAG25750.1.
    AF208856 mRNA. Translation: AAF64270.1. Sequence problems.
    AK126146 mRNA. Translation: BAC86462.1.
    AK090981 mRNA. Translation: BAG52256.1.
    AC005332 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89050.1.
    BC050709 mRNA. Translation: AAH50709.1.
    BC056271 mRNA. Translation: AAH56271.1.
    CCDSiCCDS11674.1. [Q86W34-4]
    CCDS32714.1. [Q86W34-5]
    RefSeqiNP_001028741.1. NM_001033569.1. [Q86W34-4]
    NP_001028742.1. NM_001033570.1. [Q86W34-4]
    NP_001028743.1. NM_001033571.1. [Q86W34-4]
    NP_001028744.1. NM_001033572.1. [Q86W34-4]
    NP_001028746.1. NM_001033574.1. [Q86W34-5]
    NP_001275983.1. NM_001289054.1. [Q86W34-4]
    NP_001275985.1. NM_001289056.1. [Q86W34-4]
    NP_057711.3. NM_016627.4. [Q86W34-4]
    XP_005257490.1. XM_005257433.2. [Q86W34-4]
    XP_005257492.1. XM_005257435.2. [Q86W34-4]
    XP_005257493.1. XM_005257436.1. [Q86W34-4]
    XP_005257494.1. XM_005257437.1. [Q86W34-4]
    UniGeneiHs.293560.

    Genome annotation databases

    EnsembliENST00000359783; ENSP00000352831; ENSG00000196704. [Q86W34-5]
    ENST00000359904; ENSP00000352976; ENSG00000196704. [Q86W34-4]
    ENST00000392720; ENSP00000376481; ENSG00000196704. [Q86W34-4]
    ENST00000577866; ENSP00000464133; ENSG00000196704. [Q86W34-4]
    ENST00000577985; ENSP00000464635; ENSG00000196704. [Q86W34-4]
    ENST00000580753; ENSP00000463012; ENSG00000196704. [Q86W34-4]
    GeneIDi51321.
    KEGGihsa:51321.
    UCSCiuc002jgr.1. human. [Q86W34-4]

    Polymorphism databases

    DMDMi317373321.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ635358 mRNA. Translation: CAG25750.1 .
    AF208856 mRNA. Translation: AAF64270.1 . Sequence problems.
    AK126146 mRNA. Translation: BAC86462.1 .
    AK090981 mRNA. Translation: BAG52256.1 .
    AC005332 Genomic DNA. No translation available.
    CH471099 Genomic DNA. Translation: EAW89050.1 .
    BC050709 mRNA. Translation: AAH50709.1 .
    BC056271 mRNA. Translation: AAH56271.1 .
    CCDSi CCDS11674.1. [Q86W34-4 ]
    CCDS32714.1. [Q86W34-5 ]
    RefSeqi NP_001028741.1. NM_001033569.1. [Q86W34-4 ]
    NP_001028742.1. NM_001033570.1. [Q86W34-4 ]
    NP_001028743.1. NM_001033571.1. [Q86W34-4 ]
    NP_001028744.1. NM_001033572.1. [Q86W34-4 ]
    NP_001028746.1. NM_001033574.1. [Q86W34-5 ]
    NP_001275983.1. NM_001289054.1. [Q86W34-4 ]
    NP_001275985.1. NM_001289056.1. [Q86W34-4 ]
    NP_057711.3. NM_016627.4. [Q86W34-4 ]
    XP_005257490.1. XM_005257433.2. [Q86W34-4 ]
    XP_005257492.1. XM_005257435.2. [Q86W34-4 ]
    XP_005257493.1. XM_005257436.1. [Q86W34-4 ]
    XP_005257494.1. XM_005257437.1. [Q86W34-4 ]
    UniGenei Hs.293560.

    3D structure databases

    ProteinModelPortali Q86W34.
    SMRi Q86W34. Positions 144-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119472. 2 interactions.

    Protein family/group databases

    MEROPSi M54.002.

    PTM databases

    PhosphoSitei Q86W34.

    Polymorphism databases

    DMDMi 317373321.

    Proteomic databases

    MaxQBi Q86W34.
    PaxDbi Q86W34.
    PRIDEi Q86W34.

    Protocols and materials databases

    DNASUi 51321.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000359783 ; ENSP00000352831 ; ENSG00000196704 . [Q86W34-5 ]
    ENST00000359904 ; ENSP00000352976 ; ENSG00000196704 . [Q86W34-4 ]
    ENST00000392720 ; ENSP00000376481 ; ENSG00000196704 . [Q86W34-4 ]
    ENST00000577866 ; ENSP00000464133 ; ENSG00000196704 . [Q86W34-4 ]
    ENST00000577985 ; ENSP00000464635 ; ENSG00000196704 . [Q86W34-4 ]
    ENST00000580753 ; ENSP00000463012 ; ENSG00000196704 . [Q86W34-4 ]
    GeneIDi 51321.
    KEGGi hsa:51321.
    UCSCi uc002jgr.1. human. [Q86W34-4 ]

    Organism-specific databases

    CTDi 51321.
    GeneCardsi GC17P066244.
    HGNCi HGNC:28041. AMZ2.
    HPAi HPA023299.
    HPA052406.
    MIMi 615169. gene.
    neXtProti NX_Q86W34.
    PharmGKBi PA162376414.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1913.
    HOGENOMi HOG000293350.
    HOVERGENi HBG080165.
    InParanoidi Q86W34.
    KOi K06974.
    OMAi KLQCAIG.
    OrthoDBi EOG7WX08V.
    PhylomeDBi Q86W34.
    TreeFami TF328603.

    Miscellaneous databases

    ChiTaRSi AMZ2. human.
    GenomeRNAii 51321.
    NextBioi 54701.
    PROi Q86W34.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86W34.
    Bgeei Q86W34.
    CleanExi HS_AMZ2.
    Genevestigatori Q86W34.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR012962. Pept_M54_archaemetzincn.
    [Graphical view ]
    Pfami PF07998. Peptidase_M54. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea."
      Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C.
      J. Biol. Chem. 280:30367-30375(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, VARIANT ASP-30.
    2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
      Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
      , Gu J., Chen S.-J., Chen Z.
      Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
      Tissue: Umbilical cord blood.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
      Tissue: Amygdala and Thymus.
    4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
      Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
      , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
      Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-30.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
      Tissue: Bone marrow and Lung.
    7. "Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue."
      Lin Y.H., Lin Y.M., Teng Y.N., Hsieh T.Y., Lin Y.S., Kuo P.L.
      Fertil. Steril. 86:1650-1658(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAMZ2_HUMAN
    AccessioniPrimary (citable) accession number: Q86W34
    Secondary accession number(s): A6NLD9
    , B3KR44, Q5XKF1, Q9NZE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Peptidase families
      Classification of peptidase families and list of entries
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3