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Protein

Archaemetzincin-2

Gene

AMZ2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Zinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze either neurogranin or angiotensin-2.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic, whereas the other seems to have a structural role.By similarity

Enzyme regulationi

Inhibited by the general metalloprotease inhibitors o-phenanthroline and batimastat. Also significantly inhibited by amastatin, which is an inhibitor of aminopeptidases. Not inhibited by 4-(2-aminoethyl)-benzenesulfonyl fluoride, E-64, and TIMPS (tissue inhibitors of metalloproteinases), which are inhibitors of serine, cysteine, and matrix metalloproteases, respectively.1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi254 – 2541Zinc 1; catalytic; via tele nitrogenBy similarity
Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
Metal bindingi258 – 2581Zinc 1; catalytic; via tele nitrogenBy similarity
Metal bindingi264 – 2641Zinc 1; catalytic; via tele nitrogenBy similarity
Metal bindingi265 – 2651Zinc 2By similarity
Metal bindingi270 – 2701Zinc 2By similarity
Metal bindingi289 – 2891Zinc 2By similarity
Metal bindingi292 – 2921Zinc 2By similarity

GO - Molecular functioni

  1. metallopeptidase activity Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM54.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Archaemetzincin-2 (EC:3.4.-.-)
Alternative name(s):
Archeobacterial metalloproteinase-like protein 2
Gene namesi
Name:AMZ2
ORF Names:BM-014
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:28041. AMZ2.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162376414.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 360360Archaemetzincin-2PRO_0000159617Add
BLAST

Proteomic databases

MaxQBiQ86W34.
PaxDbiQ86W34.
PRIDEiQ86W34.

PTM databases

PhosphoSiteiQ86W34.

Expressioni

Tissue specificityi

Predominantly expressed in heart and testis. Also expressed at lower levels in kidney, liver, pancreas, lung, brain and placenta. Expressed in fetal tissues such as kidney, liver, lung and brain. Down-regulated in testis from patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS).2 Publications

Gene expression databases

BgeeiQ86W34.
CleanExiHS_AMZ2.
ExpressionAtlasiQ86W34. baseline and differential.
GenevestigatoriQ86W34.

Organism-specific databases

HPAiHPA023299.
HPA052406.

Interactioni

Protein-protein interaction databases

BioGridi119472. 8 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ86W34.
SMRiQ86W34. Positions 144-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M54 family.Curated

Phylogenomic databases

eggNOGiCOG1913.
GeneTreeiENSGT00530000063996.
HOGENOMiHOG000293350.
HOVERGENiHBG080165.
InParanoidiQ86W34.
KOiK06974.
OMAiKLQCAIG.
OrthoDBiEOG7WX08V.
PhylomeDBiQ86W34.
TreeFamiTF328603.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view]
PfamiPF07998. Peptidase_M54. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86W34-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQIIRHSEQT LKTALISKNP VLVSQYEKLN AGEQRLMNEA FQPASDLFGP
60 70 80 90 100
ITLHSPSDWI TSHPEAPQDF EQFFSDPYRK TPSPNKRSIY IQSIGSLGNT
110 120 130 140 150
RIISEEYIKW LTGYCKAYFY GLRVKLLEPV PVSVTRCSFR VNENTHNLQI
160 170 180 190 200
HAGDILKFLK KKKPEDAFCV VGITMIDLYP RDSWNFVFGQ ASLTDGVGIF
210 220 230 240 250
SFARYGSDFY SMHYKGKVKK LKKTSSSDYS IFDNYYIPEI TSVLLLRSCK
260 270 280 290 300
TLTHEIGHIF GLRHCQWLAC LMQGSNHLEE ADRRPLNLCP ICLHKLQCAV
310 320 330 340 350
GFSIVERYKA LVRWIDDESS DTPGATPEHS HEDNGNLPKP VEAFKEWKEW
360
IIKCLAVLQK
Length:360
Mass (Da):41,263
Last modified:January 11, 2011 - v2
Checksum:iD74E7F7CE7873CBF
GO
Isoform 2 (identifier: Q86W34-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-153: Missing.

Note: Gene prediction based on EST data.

Show »
Length:302
Mass (Da):34,595
Checksum:i2AE9FB8B77CC8293
GO

Sequence cautioni

The sequence AAF64270.1 differs from that shown. Reason: Frameshift at position 55. Curated
The sequence AAF64270.1 differs from that shown. Reason: Erroneous termination at position 98. Translated as Gly.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41I → V in AAF64270 (PubMed:11042152).Curated
Sequence conflicti6 – 61H → Y in AAF64270 (PubMed:11042152).Curated
Sequence conflicti13 – 131T → I in AAF64270 (PubMed:11042152).Curated
Sequence conflicti15 – 151L → V in AAF64270 (PubMed:11042152).Curated
Sequence conflicti29 – 291L → V in AAF64270 (PubMed:11042152).Curated
Sequence conflicti51 – 522IT → CI in AAF64270 (PubMed:11042152).Curated
Sequence conflicti76 – 761D → H in AAF64270 (PubMed:11042152).Curated
Sequence conflicti81 – 811T → I in AAF64270 (PubMed:11042152).Curated
Sequence conflicti85 – 851N → D in AAF64270 (PubMed:11042152).Curated
Sequence conflicti92 – 921Q → R in AAF64270 (PubMed:11042152).Curated
Sequence conflicti99 – 991N → S in AAF64270 (PubMed:11042152).Curated
Sequence conflicti121 – 1211G → R in AAF64270 (PubMed:11042152).Curated
Sequence conflicti207 – 2071S → G in AAF64270 (PubMed:11042152).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301N → D.5 Publications
Corresponds to variant rs3213690 [ dbSNP | Ensembl ].
VAR_024850
Natural varianti146 – 1461H → Q.
Corresponds to variant rs3207194 [ dbSNP | Ensembl ].
VAR_047343

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei96 – 15358Missing in isoform 2. CuratedVSP_047263Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635358 mRNA. Translation: CAG25750.1.
AF208856 mRNA. Translation: AAF64270.1. Sequence problems.
AK126146 mRNA. Translation: BAC86462.1.
AK090981 mRNA. Translation: BAG52256.1.
AC005332 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89050.1.
BC050709 mRNA. Translation: AAH50709.1.
BC056271 mRNA. Translation: AAH56271.1.
CCDSiCCDS11674.1. [Q86W34-4]
CCDS32714.1. [Q86W34-5]
RefSeqiNP_001028741.1. NM_001033569.1. [Q86W34-4]
NP_001028742.1. NM_001033570.1. [Q86W34-4]
NP_001028743.1. NM_001033571.1. [Q86W34-4]
NP_001028744.1. NM_001033572.1. [Q86W34-4]
NP_001028746.1. NM_001033574.1. [Q86W34-5]
NP_001275983.1. NM_001289054.1. [Q86W34-4]
NP_001275985.1. NM_001289056.1. [Q86W34-4]
NP_057711.3. NM_016627.4. [Q86W34-4]
XP_005257490.1. XM_005257433.2. [Q86W34-4]
XP_005257492.1. XM_005257435.2. [Q86W34-4]
XP_005257493.1. XM_005257436.1. [Q86W34-4]
XP_005257494.1. XM_005257437.1. [Q86W34-4]
UniGeneiHs.293560.

Genome annotation databases

EnsembliENST00000359783; ENSP00000352831; ENSG00000196704. [Q86W34-5]
ENST00000359904; ENSP00000352976; ENSG00000196704. [Q86W34-4]
ENST00000392720; ENSP00000376481; ENSG00000196704. [Q86W34-4]
ENST00000577866; ENSP00000464133; ENSG00000196704. [Q86W34-4]
ENST00000577985; ENSP00000464635; ENSG00000196704. [Q86W34-4]
ENST00000580753; ENSP00000463012; ENSG00000196704. [Q86W34-4]
ENST00000612294; ENSP00000483162; ENSG00000196704. [Q86W34-4]
GeneIDi51321.
KEGGihsa:51321.
UCSCiuc002jgr.1. human. [Q86W34-4]

Polymorphism databases

DMDMi317373321.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ635358 mRNA. Translation: CAG25750.1.
AF208856 mRNA. Translation: AAF64270.1. Sequence problems.
AK126146 mRNA. Translation: BAC86462.1.
AK090981 mRNA. Translation: BAG52256.1.
AC005332 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89050.1.
BC050709 mRNA. Translation: AAH50709.1.
BC056271 mRNA. Translation: AAH56271.1.
CCDSiCCDS11674.1. [Q86W34-4]
CCDS32714.1. [Q86W34-5]
RefSeqiNP_001028741.1. NM_001033569.1. [Q86W34-4]
NP_001028742.1. NM_001033570.1. [Q86W34-4]
NP_001028743.1. NM_001033571.1. [Q86W34-4]
NP_001028744.1. NM_001033572.1. [Q86W34-4]
NP_001028746.1. NM_001033574.1. [Q86W34-5]
NP_001275983.1. NM_001289054.1. [Q86W34-4]
NP_001275985.1. NM_001289056.1. [Q86W34-4]
NP_057711.3. NM_016627.4. [Q86W34-4]
XP_005257490.1. XM_005257433.2. [Q86W34-4]
XP_005257492.1. XM_005257435.2. [Q86W34-4]
XP_005257493.1. XM_005257436.1. [Q86W34-4]
XP_005257494.1. XM_005257437.1. [Q86W34-4]
UniGeneiHs.293560.

3D structure databases

ProteinModelPortaliQ86W34.
SMRiQ86W34. Positions 144-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119472. 8 interactions.

Protein family/group databases

MEROPSiM54.002.

PTM databases

PhosphoSiteiQ86W34.

Polymorphism databases

DMDMi317373321.

Proteomic databases

MaxQBiQ86W34.
PaxDbiQ86W34.
PRIDEiQ86W34.

Protocols and materials databases

DNASUi51321.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359783; ENSP00000352831; ENSG00000196704. [Q86W34-5]
ENST00000359904; ENSP00000352976; ENSG00000196704. [Q86W34-4]
ENST00000392720; ENSP00000376481; ENSG00000196704. [Q86W34-4]
ENST00000577866; ENSP00000464133; ENSG00000196704. [Q86W34-4]
ENST00000577985; ENSP00000464635; ENSG00000196704. [Q86W34-4]
ENST00000580753; ENSP00000463012; ENSG00000196704. [Q86W34-4]
ENST00000612294; ENSP00000483162; ENSG00000196704. [Q86W34-4]
GeneIDi51321.
KEGGihsa:51321.
UCSCiuc002jgr.1. human. [Q86W34-4]

Organism-specific databases

CTDi51321.
GeneCardsiGC17P066244.
HGNCiHGNC:28041. AMZ2.
HPAiHPA023299.
HPA052406.
MIMi615169. gene.
neXtProtiNX_Q86W34.
PharmGKBiPA162376414.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1913.
GeneTreeiENSGT00530000063996.
HOGENOMiHOG000293350.
HOVERGENiHBG080165.
InParanoidiQ86W34.
KOiK06974.
OMAiKLQCAIG.
OrthoDBiEOG7WX08V.
PhylomeDBiQ86W34.
TreeFamiTF328603.

Miscellaneous databases

ChiTaRSiAMZ2. human.
GenomeRNAii51321.
NextBioi54701.
PROiQ86W34.
SOURCEiSearch...

Gene expression databases

BgeeiQ86W34.
CleanExiHS_AMZ2.
ExpressionAtlasiQ86W34. baseline and differential.
GenevestigatoriQ86W34.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
InterProiIPR024079. MetalloPept_cat_dom.
IPR012962. Pept_M54_archaemetzincn.
[Graphical view]
PfamiPF07998. Peptidase_M54. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of human archaemetzincin-1 and - 2, two novel members of a family of metalloproteases widely distributed in Archaea."
    Diaz-Perales A., Quesada V., Peinado J.R., Ugalde A.P., Alvarez J., Suarez M.F., Gomis-Rueth X., Lopez-Otin C.
    J. Biol. Chem. 280:30367-30375(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, VARIANT ASP-30.
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Umbilical cord blood.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Amygdala and Thymus.
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASP-30.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-30.
    Tissue: Bone marrow and Lung.
  7. "Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue."
    Lin Y.H., Lin Y.M., Teng Y.N., Hsieh T.Y., Lin Y.S., Kuo P.L.
    Fertil. Steril. 86:1650-1658(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiAMZ2_HUMAN
AccessioniPrimary (citable) accession number: Q86W34
Secondary accession number(s): A6NLD9
, B3KR44, Q5XKF1, Q9NZE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: January 11, 2011
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.