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Protein

NACHT, LRR and PYD domains-containing protein 10

Gene

NLRP10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID. Displays anti-inflammatory activity. Plays a role in adaptive immunity through control of dendritic cell-mediated transport of antigen to the lymph nodes from peripheral sites. Required for immunity against C.albicans infection. Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi173 – 1808ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NACHT, LRR and PYD domains-containing protein 10
Alternative name(s):
Nucleotide-binding oligomerization domain protein 8
Gene namesi
Name:NLRP10
Synonyms:NALP10, NOD8, PYNOD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:21464. NLRP10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extrinsic component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi179 – 1791K → A: Reduced release of IL8 following invasive bacterial infection. 1 Publication
Mutagenesisi252 – 2521D → A: Reduced release of IL8 following invasive bacterial infection. 1 Publication

Organism-specific databases

PharmGKBiPA162397832.

Polymorphism and mutation databases

BioMutaiNLRP10.
DMDMi46396380.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655NACHT, LRR and PYD domains-containing protein 10PRO_0000080896Add
BLAST

Proteomic databases

PaxDbiQ86W26.
PRIDEiQ86W26.

PTM databases

iPTMnetiQ86W26.
PhosphoSiteiQ86W26.

Expressioni

Tissue specificityi

Highly expressed in basal and suprabasal epidermal cell layers with lower levels in dermal fibroblast cells (at protein level). Widely expressed with highest levels in heart, brain and skeletal muscle. Also expressed in liver, colon, dermis and epidermis. Little expression detected in myeloid cells or peripheral blood mononuclear cells.2 Publications

Gene expression databases

BgeeiQ86W26.
CleanExiHS_NLRP10.
ExpressionAtlasiQ86W26. baseline and differential.
GenevisibleiQ86W26. HS.

Organism-specific databases

HPAiHPA039498.
HPA040101.

Interactioni

Subunit structurei

Oligomerizes. Interacts with PYCARD, CASP1 and IL1B. Also interacts with NOD1 and components of the NOD1 signaling pathway including RIPK2, NR2C2/TAK1 and IKBKG/NEMO.2 Publications

Protein-protein interaction databases

BioGridi130709. 3 interactions.
STRINGi9606.ENSP00000327763.

Structurei

Secondary structure

1
655
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 1911Combined sources
Helixi22 – 3615Combined sources
Helixi46 – 483Combined sources
Turni49 – 513Combined sources
Helixi54 – 6411Combined sources
Helixi67 – 7913Combined sources
Turni80 – 823Combined sources
Helixi84 – 9310Combined sources
Helixi96 – 983Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M5VNMR-A1-100[»]
ProteinModelPortaliQ86W26.
SMRiQ86W26. Positions 1-100, 165-190.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9696PyrinPROSITE-ProRule annotationAdd
BLAST
Domaini167 – 484318NACHTPROSITE-ProRule annotationAdd
BLAST

Domaini

The pyrin and ATP-binding domains are required to elicit cytokine release following bacterial infection.
The NACHT domain is required for inhibition of CASP1 autoprocessing.

Sequence similaritiesi

Belongs to the NLRP family.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation
Contains 1 pyrin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IGPF. Eukaryota.
ENOG410Y19A. LUCA.
GeneTreeiENSGT00840000129675.
HOGENOMiHOG000113728.
HOVERGENiHBG052572.
InParanoidiQ86W26.
OMAiDIFMAYV.
OrthoDBiEOG7P5T07.
PhylomeDBiQ86W26.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02758. PYRIN. 1 hit.
[Graphical view]
SMARTiSM01289. PYRIN. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50824. DAPIN. 1 hit.
PS50837. NACHT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86W26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMAKARKPR EALLWALSDL EENDFKKLKF YLRDMTLSEG QPPLARGELE
60 70 80 90 100
GLIPVDLAEL LISKYGEKEA VKVVLKGLKV MNLLELVDQL SHICLHDYRE
110 120 130 140 150
VYREHVRCLE EWQEAGVNGR YNQVLLVAKP SSESPESLAC PFPEQELESV
160 170 180 190 200
TVEALFDSGE KPSLAPSLVV LQGSAGTGKT TLARKMVLDW ATGTLYPGRF
210 220 230 240 250
DYVFYVSCKE VVLLLESKLE QLLFWCCGDN QAPVTEILRQ PERLLFILDG
260 270 280 290 300
FDELQRPFEE KLKKRGLSPK ESLLHLLIRR HTLPTCSLLI TTRPLALRNL
310 320 330 340 350
EPLLKQARHV HILGFSEEER ARYFSSYFTD EKQADRAFDI VQKNDILYKA
360 370 380 390 400
CQVPGICWVV CSWLQGQMER GKVVLETPRN STDIFMAYVS TFLPPDDDGG
410 420 430 440 450
CSELSRHRVL RSLCSLAAEG IQHQRFLFEE AELRKHNLDG PRLAAFLSSN
460 470 480 490 500
DYQLGLAIKK FYSFRHISFQ DFFHAMSYLV KEDQSRLGKE SRREVQRLLE
510 520 530 540 550
VKEQEGNDEM TLTMQFLLDI SKKDSFSNLE LKFCFRISPC LAQDLKHFKE
560 570 580 590 600
QMESMKHNRT WDLEFSLYEA KIKNLVKGIQ MNNVSFKIKH SNEKKSQSQN
610 620 630 640 650
LFSVKSSLSH GPKEEQKCPS VHGQKEGKDN IAGTQKEAST GKGRGTEETP

KNTYI
Length:655
Mass (Da):75,032
Last modified:June 1, 2003 - v1
Checksum:i0A4E5936864F4B5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY154465 mRNA. Translation: AAO18161.1.
AY489192 mRNA. Translation: AAS67384.1.
BK001110 mRNA. Translation: DAA01243.1.
BC104957 mRNA. Translation: AAI04958.1.
CCDSiCCDS7784.1.
RefSeqiNP_789791.1. NM_176821.3.
XP_011518345.1. XM_011520043.1.
XP_011544883.1. XM_011546581.1.
XP_011547170.1. XM_011548868.1.
UniGeneiHs.449636.

Genome annotation databases

EnsembliENST00000328600; ENSP00000327763; ENSG00000182261.
ENST00000612291; ENSP00000478480; ENSG00000276780.
ENST00000625327; ENSP00000486762; ENSG00000281166.
GeneIDi338322.
KEGGihsa:338322.
UCSCiuc001mfv.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY154465 mRNA. Translation: AAO18161.1.
AY489192 mRNA. Translation: AAS67384.1.
BK001110 mRNA. Translation: DAA01243.1.
BC104957 mRNA. Translation: AAI04958.1.
CCDSiCCDS7784.1.
RefSeqiNP_789791.1. NM_176821.3.
XP_011518345.1. XM_011520043.1.
XP_011544883.1. XM_011546581.1.
XP_011547170.1. XM_011548868.1.
UniGeneiHs.449636.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M5VNMR-A1-100[»]
ProteinModelPortaliQ86W26.
SMRiQ86W26. Positions 1-100, 165-190.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi130709. 3 interactions.
STRINGi9606.ENSP00000327763.

PTM databases

iPTMnetiQ86W26.
PhosphoSiteiQ86W26.

Polymorphism and mutation databases

BioMutaiNLRP10.
DMDMi46396380.

Proteomic databases

PaxDbiQ86W26.
PRIDEiQ86W26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000328600; ENSP00000327763; ENSG00000182261.
ENST00000612291; ENSP00000478480; ENSG00000276780.
ENST00000625327; ENSP00000486762; ENSG00000281166.
GeneIDi338322.
KEGGihsa:338322.
UCSCiuc001mfv.2. human.

Organism-specific databases

CTDi338322.
GeneCardsiNLRP10.
HGNCiHGNC:21464. NLRP10.
HPAiHPA039498.
HPA040101.
MIMi609662. gene.
neXtProtiNX_Q86W26.
PharmGKBiPA162397832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGPF. Eukaryota.
ENOG410Y19A. LUCA.
GeneTreeiENSGT00840000129675.
HOGENOMiHOG000113728.
HOVERGENiHBG052572.
InParanoidiQ86W26.
OMAiDIFMAYV.
OrthoDBiEOG7P5T07.
PhylomeDBiQ86W26.

Miscellaneous databases

GeneWikiiNLRP10.
GenomeRNAii338322.
NextBioi96929.
PROiQ86W26.
SOURCEiSearch...

Gene expression databases

BgeeiQ86W26.
CleanExiHS_NLRP10.
ExpressionAtlasiQ86W26. baseline and differential.
GenevisibleiQ86W26. HS.

Family and domain databases

Gene3Di1.10.533.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR004020. DAPIN.
IPR011029. DEATH-like_dom.
IPR007111. NACHT_NTPase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02758. PYRIN. 1 hit.
[Graphical view]
SMARTiSM01289. PYRIN. 1 hit.
[Graphical view]
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50824. DAPIN. 1 hit.
PS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NALPs: a novel protein family involved in inflammation."
    Tschopp J., Martinon F., Burns K.
    Nat. Rev. Mol. Cell Biol. 4:95-104(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "PYNOD, a novel Apaf-1/CED4-like protein is an inhibitor of ASC and caspase-1."
    Wang Y., Hasegawa M., Imamura R., Kinoshita T., Kondo C., Konaka K., Suda T.
    Int. Immunol. 16:777-786(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, OLIGOMERIZATION, INTERACTION WITH PYCARD; CASP1 AND IL1B, TISSUE SPECIFICITY.
    Tissue: Heart.
  3. "NODs: intracellular proteins involved in inflammation and apoptosis."
    Inohara N., Nunez G.
    Nat. Rev. Immunol. 3:371-382(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Anti-inflammatory activity of PYNOD and its mechanism in humans and mice."
    Imamura R., Wang Y., Kinoshita T., Suzuki M., Noda T., Sagara J., Taniguchi S., Okamoto H., Suda T.
    J. Immunol. 184:5874-5884(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: FUNCTION, INTERACTION WITH IKBKG; NOD1; NR2C2 AND RIPK2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF LYS-179 AND ASP-252.
  7. "(1)H, (13)C and (15)N resonance assignments of the pyrin domain from human PYNOD."
    Su M.Y., Chang C.I., Chang C.F.
    Biomol. NMR. Assign. 7:141-143(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-100.

Entry informationi

Entry nameiNAL10_HUMAN
AccessioniPrimary (citable) accession number: Q86W26
Secondary accession number(s): Q2M3C4, Q6JGT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Despite its official name, does not contain LRR repeats.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.