Q86VS8 (HOOK3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Hook homolog 3
Short name=h-hook3
Short name=hHK3

Gene names

Name:

HOOK3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	718 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking By similarity. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Ref.1 Ref.6 Ref.7
Subunit structure	Interacts with Salmonella typhimurium spiC By similarity. Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK2. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with MSR1, and this association is stimulated by ligand binding to MSR1. Interacts with microtubules. Ref.1 Ref.6 Ref.7
Subcellular location	Cytoplasm › cytoskeleton. Golgi apparatus. Note: Enriched at the cis-face of the Golgi complex By similarity. Localizes to microtubule asters in prophase By similarity. Ref.1
Sequence similarities	Belongs to the hook family.
Sequence caution	The sequence AAH48304.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Golgi apparatus Microtubule
Coding sequence diversity	Polymorphism
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi localization Inferred from mutant phenotype Ref.1. Source: UniProtKB cytoplasmic microtubule organization Inferred from mutant phenotype Ref.1. Source: UniProtKB early endosome to late endosome transport Inferred from mutant phenotype Ref.7. Source: UniProtKB endosome organization Inferred from mutant phenotype Ref.7. Source: UniProtKB endosome to lysosome transport Inferred from mutant phenotype Ref.7. Source: UniProtKB interkinetic nuclear migration Inferred from sequence or structural similarity. Source: BHF-UCL lysosome organization Inferred from mutant phenotype Ref.7. Source: UniProtKB microtubule anchoring at centrosome Inferred from sequence or structural similarity. Source: BHF-UCL negative regulation of neurogenesis Inferred from sequence or structural similarity. Source: BHF-UCL neuronal stem cell maintenance Inferred from electronic annotation. Source: Compara protein localization to centrosome Inferred from sequence or structural similarity. Source: BHF-UCL protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	FHF complex Inferred from direct assay Ref.7. Source: UniProtKB Golgi apparatus Inferred from direct assay. Source: HPA centriolar satellite Inferred from electronic annotation. Source: Compara cis-Golgi network Inferred from direct assay Ref.1. Source: UniProtKB microtubule Inferred from electronic annotation. Source: UniProtKB-KW pericentriolar material Inferred from sequence or structural similarity. Source: BHF-UCL
Molecular_function	microtubule binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MSR1	P21757	4	EBI-1777078,EBI-1776976

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 718	718	Protein Hook homolog 3		PRO_0000219197
Regions
	Region	1 – 164	164	Sufficient for interaction with microtubules
	Region	553 – 718	166	Required for association with Golgi
	Region	556 – 718	163	Required for interaction with MSR1
	Coiled coil	167 – 433	267	Potential
	Coiled coil	462 – 667	206	Potential
Amino acid modifications
	Modified residue	238	1	Phosphoserine Ref.5 Ref.8 Ref.9
Natural variations
	Natural variant	221	1	Q → R in a breast cancer sample; somatic mutation. Ref.11		VAR_035710
	Natural variant	670	1	Y → S. Corresponds to variant rs34131505 [ dbSNP | Ensembl ].		VAR_049363
Experimental info
	Sequence conflict	348	1	M → V in BAC03473. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q86VS8 [UniParc]. Last modified February 2, 2004. Version 2. Checksum: 9528EC9C854D39FA	FASTA	718	83,126
	10 20 30 40 50 60 MFSVESLERA ELCESLLTWI QTFNVDAPCQ TVEDLTNGVV MAQVLQKIDP AYFDENWLNR 70 80 90 100 110 120 IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL PDVNLIGEHS DAAELGRMLQ 130 140 150 160 170 180 LILGCAVNCE QKQEYIQAIM MMEESVQHVV MTAIQELMSK ESPVSAGNDA YVDLDRQLKK 190 200 210 220 230 240 TTEELNEALS AKEEIAQRCH ELDMQVAALQ EEKSSLLAEN QVLMERLNQS DSIEDPNSPA 250 260 270 280 290 300 GRRHLQLQTQ LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK 310 320 330 340 350 360 DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN TVSLEEELRK 370 380 390 400 410 420 ANAARSQLET YKRQVVELQN RLSEESKKAD KLDFEYKRLK EKVDSLQKEK DRLRTERDSL 430 440 450 460 470 480 KETIEELRCV QAQEGQLTTQ GLMPLGSQES SDSLAAEIVT PEIREKLIRL QHENKMLKLN 490 500 510 520 530 540 QEGSDNEKIA LLQSLLDDAN LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED 550 560 570 580 590 600 SVLLKKKLEE HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LKIEELQEAL RKKEEEMKQM 610 620 630 640 650 660 EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE YEKTKSQREM 670 680 690 700 710 EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ ATSSRRSYPG HVQPATAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins." Walenta J.H., Didier A.J., Liu X., Kraemer H. J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Cervix and Duodenum.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 346-718. Tissue: Astrocyte.
[5]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A." Sano H., Ishino M., Kraemer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. J. Biol. Chem. 282:7973-7981(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MSR1.
[7]	"An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex." Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W. Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK2; VPS16 AND VPS41.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-221.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF241830 mRNA. Translation: AAK29204.1. CH471080 Genomic DNA. Translation: EAW63197.1. CH471080 Genomic DNA. Translation: EAW63198.1. BC048304 mRNA. Translation: AAH48304.1. Sequence problems. BC056146 mRNA. Translation: AAH56146.1. AK090540 mRNA. Translation: BAC03473.1.
IPI	IPI00031768.
RefSeq	NP_115786.1. NM_032410.3.
UniGene	Hs.162852.
3D structure databases
ProteinModelPortal	Q86VS8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86VS8. 2 interactions.
STRING	9606.ENSP00000305699.
PTM databases
PhosphoSite	Q86VS8.
Polymorphism databases
DMDM	41688581.
Proteomic databases
PaxDb	Q86VS8.
PRIDE	Q86VS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000307602; ENSP00000305699; ENSG00000168172.
GeneID	84376.
KEGG	hsa:84376.
UCSC	uc003xpr.3. human.
Organism-specific databases
CTD	84376.
GeneCards	GC08P042769.
HGNC	HGNC:23576. HOOK3.
HPA	HPA024756.
MIM	607825. gene.
neXtProt	NX_Q86VS8.
PharmGKB	PA134961305.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG130433.
HOGENOM	HOG000294112.
HOVERGEN	HBG051920.
InParanoid	Q86VS8.
KO	K16536.
OMA	TIMMMEE.
OrthoDB	EOG418BN0.
PhylomeDB	Q86VS8.
Gene expression databases
ArrayExpress	Q86VS8.
Bgee	Q86VS8.
CleanEx	HS_HOOK3.
Genevestigator	Q86VS8.
GermOnline	ENSG00000168172. Homo sapiens.
Family and domain databases
InterPro	IPR008636. HOOK/CCDC88. [Graphical view]
Pfam	PF05622. HOOK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HOOK3. human.
GenomeRNAi	84376.
NextBio	74148.
SOURCE	Search...

Entry information

Entry name

HOOK3_HUMAN

Accession

Primary (citable) accession number: Q86VS8
Secondary accession number(s): D3DSY8, Q8NBH0, Q9BY13

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	February 2, 2004
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.