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Q86VS8

- HOOK3_HUMAN

UniProt

Q86VS8 - HOOK3_HUMAN

Protein

Protein Hook homolog 3

Gene

HOOK3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (02 Feb 2004)
      Previous versions | rss
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    Functioni

    Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking By similarity. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex.By similarity3 Publications

    GO - Molecular functioni

    1. identical protein binding Source: UniProtKB
    2. microtubule binding Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cytoplasmic microtubule organization Source: UniProtKB
    2. early endosome to late endosome transport Source: UniProtKB
    3. endosome organization Source: UniProtKB
    4. endosome to lysosome transport Source: UniProtKB
    5. Golgi localization Source: UniProtKB
    6. interkinetic nuclear migration Source: BHF-UCL
    7. lysosome organization Source: UniProtKB
    8. microtubule anchoring at centrosome Source: BHF-UCL
    9. negative regulation of neurogenesis Source: BHF-UCL
    10. neuronal stem cell maintenance Source: Ensembl
    11. protein localization to centrosome Source: BHF-UCL
    12. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Hook homolog 3
    Short name:
    h-hook3
    Short name:
    hHK3
    Gene namesi
    Name:HOOK3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:23576. HOOK3.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Golgi apparatus 1 Publication
    Note: Enriched at the cis-face of the Golgi complex. Localizes to microtubule asters in prophase.By similarity

    GO - Cellular componenti

    1. centriolar satellite Source: Ensembl
    2. centrosome Source: BHF-UCL
    3. cis-Golgi network Source: UniProtKB
    4. cytoplasm Source: HPA
    5. FHF complex Source: UniProtKB
    6. Golgi apparatus Source: HPA
    7. microtubule Source: UniProtKB-KW
    8. microtubule organizing center Source: HPA
    9. pericentriolar material Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134961305.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 718718Protein Hook homolog 3PRO_0000219197Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine2 Publications
    Modified residuei238 – 2381Phosphoserine3 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ86VS8.
    PaxDbiQ86VS8.
    PRIDEiQ86VS8.

    PTM databases

    PhosphoSiteiQ86VS8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86VS8.
    BgeeiQ86VS8.
    CleanExiHS_HOOK3.
    GenevestigatoriQ86VS8.

    Organism-specific databases

    HPAiHPA024756.

    Interactioni

    Subunit structurei

    Interacts with Salmonella typhimurium spiC By similarity. Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK2. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with MSR1, and this association is stimulated by ligand binding to MSR1. Interacts with microtubules.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MSR1P217574EBI-1777078,EBI-1776976

    Protein-protein interaction databases

    BioGridi124068. 11 interactions.
    IntActiQ86VS8. 2 interactions.
    STRINGi9606.ENSP00000305699.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86VS8.
    SMRiQ86VS8. Positions 12-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 164164Sufficient for interaction with microtubulesAdd
    BLAST
    Regioni553 – 718166Required for association with GolgiAdd
    BLAST
    Regioni556 – 718163Required for interaction with MSR1Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili167 – 433267Sequence AnalysisAdd
    BLAST
    Coiled coili462 – 667206Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the hook family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG130433.
    HOGENOMiHOG000294112.
    HOVERGENiHBG051920.
    InParanoidiQ86VS8.
    KOiK16536.
    OMAiELQANIM.
    OrthoDBiEOG7ZD1V5.
    PhylomeDBiQ86VS8.
    TreeFamiTF320231.

    Family and domain databases

    InterProiIPR008636. Hook-related_fam.
    [Graphical view]
    PANTHERiPTHR18947. PTHR18947. 1 hit.
    PfamiPF05622. HOOK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q86VS8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSVESLERA ELCESLLTWI QTFNVDAPCQ TVEDLTNGVV MAQVLQKIDP    50
    AYFDENWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL 100
    PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV 150
    MTAIQELMSK ESPVSAGNDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH 200
    ELDMQVAALQ EEKSSLLAEN QVLMERLNQS DSIEDPNSPA GRRHLQLQTQ 250
    LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK 300
    DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN 350
    TVSLEEELRK ANAARSQLET YKRQVVELQN RLSEESKKAD KLDFEYKRLK 400
    EKVDSLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES 450
    SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEGSDNEKIA LLQSLLDDAN 500
    LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE 550
    HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LKIEELQEAL RKKEEEMKQM 600
    EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE 650
    YEKTKSQREM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ 700
    ATSSRRSYPG HVQPATAR 718
    Length:718
    Mass (Da):83,126
    Last modified:February 2, 2004 - v2
    Checksum:i9528EC9C854D39FA
    GO

    Sequence cautioni

    The sequence AAH48304.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti348 – 3481M → V in BAC03473. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti221 – 2211Q → R in a breast cancer sample; somatic mutation. 1 Publication
    VAR_035710
    Natural varianti670 – 6701Y → S.
    Corresponds to variant rs34131505 [ dbSNP | Ensembl ].
    VAR_049363

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241830 mRNA. Translation: AAK29204.1.
    CH471080 Genomic DNA. Translation: EAW63197.1.
    CH471080 Genomic DNA. Translation: EAW63198.1.
    BC048304 mRNA. Translation: AAH48304.1. Sequence problems.
    BC056146 mRNA. Translation: AAH56146.1.
    AK090540 mRNA. Translation: BAC03473.1.
    CCDSiCCDS6139.1.
    RefSeqiNP_115786.1. NM_032410.3.
    UniGeneiHs.162852.

    Genome annotation databases

    EnsembliENST00000307602; ENSP00000305699; ENSG00000168172.
    GeneIDi84376.
    KEGGihsa:84376.
    UCSCiuc003xpr.3. human.

    Polymorphism databases

    DMDMi41688581.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241830 mRNA. Translation: AAK29204.1 .
    CH471080 Genomic DNA. Translation: EAW63197.1 .
    CH471080 Genomic DNA. Translation: EAW63198.1 .
    BC048304 mRNA. Translation: AAH48304.1 . Sequence problems.
    BC056146 mRNA. Translation: AAH56146.1 .
    AK090540 mRNA. Translation: BAC03473.1 .
    CCDSi CCDS6139.1.
    RefSeqi NP_115786.1. NM_032410.3.
    UniGenei Hs.162852.

    3D structure databases

    ProteinModelPortali Q86VS8.
    SMRi Q86VS8. Positions 12-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 124068. 11 interactions.
    IntActi Q86VS8. 2 interactions.
    STRINGi 9606.ENSP00000305699.

    PTM databases

    PhosphoSitei Q86VS8.

    Polymorphism databases

    DMDMi 41688581.

    Proteomic databases

    MaxQBi Q86VS8.
    PaxDbi Q86VS8.
    PRIDEi Q86VS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000307602 ; ENSP00000305699 ; ENSG00000168172 .
    GeneIDi 84376.
    KEGGi hsa:84376.
    UCSCi uc003xpr.3. human.

    Organism-specific databases

    CTDi 84376.
    GeneCardsi GC08P042769.
    HGNCi HGNC:23576. HOOK3.
    HPAi HPA024756.
    MIMi 607825. gene.
    neXtProti NX_Q86VS8.
    PharmGKBi PA134961305.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG130433.
    HOGENOMi HOG000294112.
    HOVERGENi HBG051920.
    InParanoidi Q86VS8.
    KOi K16536.
    OMAi ELQANIM.
    OrthoDBi EOG7ZD1V5.
    PhylomeDBi Q86VS8.
    TreeFami TF320231.

    Miscellaneous databases

    ChiTaRSi HOOK3. human.
    GeneWikii HOOK3.
    GenomeRNAii 84376.
    NextBioi 74148.
    PROi Q86VS8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86VS8.
    Bgeei Q86VS8.
    CleanExi HS_HOOK3.
    Genevestigatori Q86VS8.

    Family and domain databases

    InterProi IPR008636. Hook-related_fam.
    [Graphical view ]
    PANTHERi PTHR18947. PTHR18947. 1 hit.
    Pfami PF05622. HOOK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins."
      Walenta J.H., Didier A.J., Liu X., Kraemer H.
      J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH MICROTUBULES, SUBCELLULAR LOCATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Cervix and Duodenum.
    4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 346-718.
      Tissue: Astrocyte.
    5. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
      Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
      Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A."
      Sano H., Ishino M., Kraemer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y.
      J. Biol. Chem. 282:7973-7981(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MSR1.
    7. "An FTS/Hook/p107(FHIP) complex interacts with and promotes endosomal clustering by the homotypic vacuolar protein sorting complex."
      Xu L., Sowa M.E., Chen J., Li X., Gygi S.P., Harper J.W.
      Mol. Biol. Cell 19:5059-5071(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE FHF COMPLEX, FUNCTION, SELF-ASSOCIATION, INTERACTION WITH AKTIP; HOOK1; HOOK2; VPS16 AND VPS41.
    8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-221.

    Entry informationi

    Entry nameiHOOK3_HUMAN
    AccessioniPrimary (citable) accession number: Q86VS8
    Secondary accession number(s): D3DSY8, Q8NBH0, Q9BY13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 2, 2004
    Last sequence update: February 2, 2004
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3