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Protein

Protein Hook homolog 3

Gene

HOOK3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex.By similarity3 Publications

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cytoplasmic microtubule organization Source: UniProtKB
  • early endosome to late endosome transport Source: UniProtKB
  • endosome organization Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • Golgi localization Source: UniProtKB
  • interkinetic nuclear migration Source: BHF-UCL
  • lysosome organization Source: UniProtKB
  • microtubule anchoring at centrosome Source: BHF-UCL
  • negative regulation of neurogenesis Source: BHF-UCL
  • neuronal stem cell population maintenance Source: Ensembl
  • protein localization to centrosome Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

SIGNORiQ86VS8.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 3
Short name:
h-hook3
Short name:
hHK3
Gene namesi
Name:HOOK3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:23576. HOOK3.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Golgi apparatus 1 Publication

  • Note: Enriched at the cis-face of the Golgi complex. Localizes to microtubule asters in prophase.By similarity

GO - Cellular componenti

  • centriolar satellite Source: Ensembl
  • centrosome Source: BHF-UCL
  • cis-Golgi network Source: UniProtKB
  • cytoplasm Source: HPA
  • cytosol Source: GOC
  • FHF complex Source: UniProtKB
  • Golgi apparatus Source: HPA
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: HPA
  • pericentriolar material Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

Pathology & Biotechi

Organism-specific databases

DisGeNETi84376.
OpenTargetsiENSG00000168172.
PharmGKBiPA134961305.

Polymorphism and mutation databases

BioMutaiHOOK3.
DMDMi41688581.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191971 – 718Protein Hook homolog 3Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei3PhosphoserineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei693PhosphoserineCombined sources1
Modified residuei707PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ86VS8.
MaxQBiQ86VS8.
PaxDbiQ86VS8.
PeptideAtlasiQ86VS8.
PRIDEiQ86VS8.

PTM databases

iPTMnetiQ86VS8.
PhosphoSitePlusiQ86VS8.

Expressioni

Gene expression databases

BgeeiENSG00000168172.
CleanExiHS_HOOK3.
ExpressionAtlasiQ86VS8. baseline and differential.
GenevisibleiQ86VS8. HS.

Organism-specific databases

HPAiHPA024756.

Interactioni

Subunit structurei

Interacts with Salmonella typhimurium spiC (By similarity). Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK2. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with MSR1, and this association is stimulated by ligand binding to MSR1. Interacts with microtubules.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MSR1P217574EBI-1777078,EBI-1776976

GO - Molecular functioni

  • identical protein binding Source: UniProtKB
  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124068. 30 interactors.
IntActiQ86VS8. 12 interactors.
STRINGi9606.ENSP00000305699.

Structurei

Secondary structure

1718
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 20Combined sources11
Helixi21 – 23Combined sources3
Helixi32 – 35Combined sources4
Helixi39 – 48Combined sources10
Turni50 – 52Combined sources3
Helixi55 – 58Combined sources4
Helixi69 – 89Combined sources21
Helixi103 – 109Combined sources7
Helixi112 – 128Combined sources17
Helixi132 – 157Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J8EX-ray1.70A/B1-160[»]
ProteinModelPortaliQ86VS8.
SMRiQ86VS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Sufficient for interaction with microtubulesAdd BLAST164
Regioni553 – 718Required for association with GolgiAdd BLAST166
Regioni556 – 718Required for interaction with MSR11 PublicationAdd BLAST163

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili167 – 433Sequence analysisAdd BLAST267
Coiled coili462 – 667Sequence analysisAdd BLAST206

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000294112.
HOVERGENiHBG051920.
InParanoidiQ86VS8.
KOiK16536.
OMAiICEAQLD.
OrthoDBiEOG091G06O9.
PhylomeDBiQ86VS8.
TreeFamiTF320231.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86VS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSVESLERA ELCESLLTWI QTFNVDAPCQ TVEDLTNGVV MAQVLQKIDP
60 70 80 90 100
AYFDENWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL
110 120 130 140 150
PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV
160 170 180 190 200
MTAIQELMSK ESPVSAGNDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH
210 220 230 240 250
ELDMQVAALQ EEKSSLLAEN QVLMERLNQS DSIEDPNSPA GRRHLQLQTQ
260 270 280 290 300
LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK
310 320 330 340 350
DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN
360 370 380 390 400
TVSLEEELRK ANAARSQLET YKRQVVELQN RLSEESKKAD KLDFEYKRLK
410 420 430 440 450
EKVDSLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES
460 470 480 490 500
SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEGSDNEKIA LLQSLLDDAN
510 520 530 540 550
LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE
560 570 580 590 600
HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LKIEELQEAL RKKEEEMKQM
610 620 630 640 650
EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE
660 670 680 690 700
YEKTKSQREM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ
710
ATSSRRSYPG HVQPATAR
Length:718
Mass (Da):83,126
Last modified:February 2, 2004 - v2
Checksum:i9528EC9C854D39FA
GO

Sequence cautioni

The sequence AAH48304 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti348M → V in BAC03473 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_035710221Q → R in a breast cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_049363670Y → S.Corresponds to variant rs34131505dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241830 mRNA. Translation: AAK29204.1.
CH471080 Genomic DNA. Translation: EAW63197.1.
CH471080 Genomic DNA. Translation: EAW63198.1.
BC048304 mRNA. Translation: AAH48304.1. Sequence problems.
BC056146 mRNA. Translation: AAH56146.1.
AK090540 mRNA. Translation: BAC03473.1.
CCDSiCCDS6139.1.
RefSeqiNP_115786.1. NM_032410.3.
UniGeneiHs.162852.

Genome annotation databases

EnsembliENST00000307602; ENSP00000305699; ENSG00000168172.
GeneIDi84376.
KEGGihsa:84376.
UCSCiuc003xpr.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF241830 mRNA. Translation: AAK29204.1.
CH471080 Genomic DNA. Translation: EAW63197.1.
CH471080 Genomic DNA. Translation: EAW63198.1.
BC048304 mRNA. Translation: AAH48304.1. Sequence problems.
BC056146 mRNA. Translation: AAH56146.1.
AK090540 mRNA. Translation: BAC03473.1.
CCDSiCCDS6139.1.
RefSeqiNP_115786.1. NM_032410.3.
UniGeneiHs.162852.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5J8EX-ray1.70A/B1-160[»]
ProteinModelPortaliQ86VS8.
SMRiQ86VS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124068. 30 interactors.
IntActiQ86VS8. 12 interactors.
STRINGi9606.ENSP00000305699.

PTM databases

iPTMnetiQ86VS8.
PhosphoSitePlusiQ86VS8.

Polymorphism and mutation databases

BioMutaiHOOK3.
DMDMi41688581.

Proteomic databases

EPDiQ86VS8.
MaxQBiQ86VS8.
PaxDbiQ86VS8.
PeptideAtlasiQ86VS8.
PRIDEiQ86VS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307602; ENSP00000305699; ENSG00000168172.
GeneIDi84376.
KEGGihsa:84376.
UCSCiuc003xpr.4. human.

Organism-specific databases

CTDi84376.
DisGeNETi84376.
GeneCardsiHOOK3.
HGNCiHGNC:23576. HOOK3.
HPAiHPA024756.
MIMi607825. gene.
neXtProtiNX_Q86VS8.
OpenTargetsiENSG00000168172.
PharmGKBiPA134961305.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOGENOMiHOG000294112.
HOVERGENiHBG051920.
InParanoidiQ86VS8.
KOiK16536.
OMAiICEAQLD.
OrthoDBiEOG091G06O9.
PhylomeDBiQ86VS8.
TreeFamiTF320231.

Enzyme and pathway databases

SIGNORiQ86VS8.

Miscellaneous databases

ChiTaRSiHOOK3. human.
GeneWikiiHOOK3.
GenomeRNAii84376.
PROiQ86VS8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168172.
CleanExiHS_HOOK3.
ExpressionAtlasiQ86VS8. baseline and differential.
GenevisibleiQ86VS8. HS.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHOOK3_HUMAN
AccessioniPrimary (citable) accession number: Q86VS8
Secondary accession number(s): D3DSY8, Q8NBH0, Q9BY13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.