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Protein

Multidrug and toxin extrusion protein 2

Gene

SLC47A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, and ganciclovir. Responsible for the secretion of cationic drugs across the brush border membranes.3 Publications

Kineticsi

  1. KM=0.76 mM for TEA2 Publications
  2. KM=0.11 mM for MPP2 Publications
  3. KM=0.12 mM for cimetidine2 Publications
  4. KM=1.98 mM for metformin2 Publications
  5. KM=4.2 mM for guanidine2 Publications
  6. KM=1.58 mM for procainamide2 Publications
  7. KM=0.06 mM for topotecan2 Publications
  8. KM=0.85 mM for estrone sulfate2 Publications
  9. KM=4.32 mM for acyclovir2 Publications
  10. KM=4.28 mM for ganciclovir2 Publications
  1. Vmax=0.88 nmol/min/mg enzyme toward TEA2 Publications
  2. Vmax=0.575 nmol/min/mg enzyme toward MPP2 Publications
  3. Vmax=0.115 nmol/min/mg enzyme toward cimetidine2 Publications
  4. Vmax=0.845 nmol/min/mg enzyme toward metformin2 Publications
  5. Vmax=0.58 nmol/min/mg enzyme toward guanidine2 Publications
  6. Vmax=3.385 nmol/min/mg enzyme toward procainamide2 Publications
  7. Vmax=0.13 nmol/min/mg enzyme toward topotecan2 Publications
  8. Vmax=0.425 nmol/min/mg enzyme toward estrone sulfate2 Publications
  9. Vmax=0.945 nmol/min/mg enzyme toward acyclovir2 Publications
  10. Vmax=0.805 nmol/min/mg enzyme toward ganciclovir2 Publications

pH dependencei

Optimum pH is 9.0. Active from pH 6 to 9.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-HSA-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi2.A.66.1.20. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug and toxin extrusion protein 2
Short name:
MATE-2
Short name:
hMATE-2
Alternative name(s):
Kidney-specific H(+)/organic cation antiporter
Solute carrier family 47 member 2
Gene namesi
Name:SLC47A2
Synonyms:MATE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:26439. SLC47A2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 66ExtracellularSequence analysisAdd BLAST12
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88 – 119CytoplasmicSequence analysisAdd BLAST32
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 153ExtracellularSequence analysisAdd BLAST13
Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
Topological domaini175 – 219CytoplasmicSequence analysisAdd BLAST45
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 248ExtracellularSequence analysis8
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 289CytoplasmicSequence analysisAdd BLAST20
Transmembranei290 – 309HelicalSequence analysisAdd BLAST20
Topological domaini310 – 327ExtracellularSequence analysisAdd BLAST18
Transmembranei328 – 348HelicalSequence analysisAdd BLAST21
Topological domaini349 – 368CytoplasmicSequence analysisAdd BLAST20
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Topological domaini390 – 402ExtracellularSequence analysisAdd BLAST13
Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
Topological domaini424 – 442CytoplasmicSequence analysisAdd BLAST19
Transmembranei443 – 463HelicalSequence analysisAdd BLAST21
Topological domaini464 – 466ExtracellularSequence analysis3
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Topological domaini488 – 578CytoplasmicSequence analysisAdd BLAST91
Transmembranei579 – 599HelicalSequence analysisAdd BLAST21
Topological domaini600 – 602ExtracellularSequence analysis3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi146802.
OpenTargetsiENSG00000180638.
PharmGKBiPA162403847.

Chemistry databases

ChEMBLiCHEMBL1743127.
DrugBankiDB00787. Aciclovir.
GuidetoPHARMACOLOGYi1217.

Polymorphism and mutation databases

BioMutaiSLC47A2.
DMDMi74727585.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003119521 – 602Multidrug and toxin extrusion protein 2Add BLAST602

Proteomic databases

PaxDbiQ86VL8.
PeptideAtlasiQ86VL8.
PRIDEiQ86VL8.

PTM databases

iPTMnetiQ86VL8.
PhosphoSitePlusiQ86VL8.

Expressioni

Tissue specificityi

Isoform 3 is predominantly expressed in kidney. Isoform 6 is expressed in brain.1 Publication

Gene expression databases

BgeeiENSG00000180638.
CleanExiHS_SLC47A2.
ExpressionAtlasiQ86VL8. baseline and differential.
GenevisibleiQ86VL8. HS.

Interactioni

Protein-protein interaction databases

MINTiMINT-4723030.
STRINGi9606.ENSP00000326671.

Chemistry databases

BindingDBiQ86VL8.

Structurei

3D structure databases

ProteinModelPortaliQ86VL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi508 – 511Poly-Gln4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ86VL8.
KOiK03327.
OMAiIVACYHV.
OrthoDBiEOG091G06X2.
PhylomeDBiQ86VL8.
TreeFamiTF324441.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86VL8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSLQDTVAL DHGGCCPALS RLVPRGFGTE MWTLFALSGP LFLFQVLTFM
60 70 80 90 100
IYIVSTVFCG HLGKVELASV TLAVAFVNVC GVSVGVGLSS ACDTLMSQSF
110 120 130 140 150
GSPNKKHVGV ILQRGALVLL LCCLPCWALF LNTQHILLLF RQDPDVSRLT
160 170 180 190 200
QDYVMIFIPG LPVIFLYNLL AKYLQNQGWL KGQEEESPFQ TPGLSILHPS
210 220 230 240 250
HSHLSRASFH LFQKITWPQV LSGVVGNCVN GVANYALVSV LNLGVRGSAY
260 270 280 290 300
ANIISQFAQT VFLLLYIVLK KLHLETWAGW SSQCLQDWGP FFSLAVPSML
310 320 330 340 350
MICVEWWAYE IGSFLMGLLS VVDLSAQAVI YEVATVTYMI PLGLSIGVCV
360 370 380 390 400
RVGMALGAAD TVQAKRSAVS GVLSIVGISL VLGTLISILK NQLGHIFTND
410 420 430 440 450
EDVIALVSQV LPVYSVFHVF EAICCVYGGV LRGTGKQAFG AAVNAITYYI
460 470 480 490 500
IGLPLGILLT FVVRMRIMGL WLGMLACVFL ATAAFVAYTA RLDWKLAAEE
510 520 530 540 550
AKKHSGRQQQ QRAESTATRP GPEKAVLSSV ATGSSPGITL TTYSRSECHV
560 570 580 590 600
DFFRTPEEAH ALSAPTSRLS VKQLVIRRGA ALGAASATLM VGLTVRILAT

RH
Length:602
Mass (Da):65,085
Last modified:June 1, 2003 - v1
Checksum:i88EB90286AE701D1
GO
Isoform 2 (identifier: Q86VL8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.
     178-213: Missing.
     376-381: VGISLV → EMSLPW
     382-602: Missing.

Note: No experimental confirmation available.
Show »
Length:296
Mass (Da):32,120
Checksum:iA8233F83A55432EC
GO
Isoform 3 (identifier: Q86VL8-3) [UniParc]FASTAAdd to basket
Also known as: MATE2-K

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.

Note: No experimental confirmation available.
Show »
Length:566
Mass (Da):61,016
Checksum:iA3D0B0E578E0B8C2
GO
Isoform 4 (identifier: Q86VL8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.
     339-339: M → MRHSHRLAYAAHVTR

Note: No experimental confirmation available.
Show »
Length:580
Mass (Da):62,673
Checksum:i848F216F93C7F1C7
GO
Isoform 5 (identifier: Q86VL8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     178-213: Missing.
     317-398: GLLSVVDLSA...LKNQLGHIFT → EHAQCGGSLC...EKSAGAYFYQ
     399-602: Missing.

Note: No experimental confirmation available.
Show »
Length:362
Mass (Da):39,287
Checksum:i35A2CE7A78E3E19D
GO
Isoform 6 (identifier: Q86VL8-6) [UniParc]FASTAAdd to basket
Also known as: MATE2-B

The sequence of this isoform differs from the canonical sequence as follows:
     178-219: GWLKGQEEES...HLFQKITWPQ → AGERLCVPAS...VHPPSISLTP
     220-602: Missing.

Note: Inactive. Note=No experimental confirmation available.
Show »
Length:219
Mass (Da):23,344
Checksum:i40D6F1AA297DAA91
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6D → E in BAF37007 (PubMed:16807400).Curated1
Sequence conflicti23V → A in AK055758 (PubMed:14702039).Curated1
Sequence conflicti93D → G in AK055758 (PubMed:14702039).Curated1
Sequence conflicti284C → Y in CAH56154 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_037358429G → R.Corresponds to variant rs34399035dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0296561 – 49Missing in isoform 2. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_029657178 – 219GWLKG…ITWPQ → AGERLCVPASSNSSTGMAEG AGGGVPIPNPGFVHPPSISL TP in isoform 6. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_029658178 – 213Missing in isoform 2, isoform 3, isoform 4 and isoform 5. 4 PublicationsAdd BLAST36
Alternative sequenceiVSP_029659220 – 602Missing in isoform 6. 1 PublicationAdd BLAST383
Alternative sequenceiVSP_029660317 – 398GLLSV…GHIFT → EHAQCGGSLCPGCHLGGHCD LHDSLGAQHRGLCPSGDGSG GRGYCAGQALGRLGRAQHSW HFPGPGHPDKHPEKSAGAYF YQ in isoform 5. 1 PublicationAdd BLAST82
Alternative sequenceiVSP_029662339M → MRHSHRLAYAAHVTR in isoform 4. 1 Publication1
Alternative sequenceiVSP_029663376 – 381VGISLV → EMSLPW in isoform 2. 1 Publication6
Alternative sequenceiVSP_029664382 – 602Missing in isoform 2. 1 PublicationAdd BLAST221
Alternative sequenceiVSP_029661399 – 602Missing in isoform 5. 1 PublicationAdd BLAST204

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250364 mRNA. Translation: BAF36847.1.
AB250701 mRNA. Translation: BAF37007.1.
AK055758 mRNA. No translation available.
BX648861 mRNA. Translation: CAH56154.1.
CH471212 Genomic DNA. Translation: EAW50902.1.
CH471212 Genomic DNA. Translation: EAW50904.1.
CH471212 Genomic DNA. Translation: EAW50905.1.
BC035288 mRNA. Translation: AAH35288.1.
BC050578 mRNA. Translation: AAH50578.1.
CCDSiCCDS11211.1. [Q86VL8-1]
CCDS58530.1. [Q86VL8-4]
RefSeqiNP_001093116.1. NM_001099646.1. [Q86VL8-3]
NP_001243592.1. NM_001256663.1. [Q86VL8-4]
NP_690872.2. NM_152908.3. [Q86VL8-1]
UniGeneiHs.126830.

Genome annotation databases

EnsembliENST00000325411; ENSP00000326671; ENSG00000180638. [Q86VL8-1]
ENST00000350657; ENSP00000338084; ENSG00000180638. [Q86VL8-4]
ENST00000574239; ENSP00000458694; ENSG00000180638. [Q86VL8-6]
GeneIDi146802.
KEGGihsa:146802.
UCSCiuc002gwe.5. human. [Q86VL8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250364 mRNA. Translation: BAF36847.1.
AB250701 mRNA. Translation: BAF37007.1.
AK055758 mRNA. No translation available.
BX648861 mRNA. Translation: CAH56154.1.
CH471212 Genomic DNA. Translation: EAW50902.1.
CH471212 Genomic DNA. Translation: EAW50904.1.
CH471212 Genomic DNA. Translation: EAW50905.1.
BC035288 mRNA. Translation: AAH35288.1.
BC050578 mRNA. Translation: AAH50578.1.
CCDSiCCDS11211.1. [Q86VL8-1]
CCDS58530.1. [Q86VL8-4]
RefSeqiNP_001093116.1. NM_001099646.1. [Q86VL8-3]
NP_001243592.1. NM_001256663.1. [Q86VL8-4]
NP_690872.2. NM_152908.3. [Q86VL8-1]
UniGeneiHs.126830.

3D structure databases

ProteinModelPortaliQ86VL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4723030.
STRINGi9606.ENSP00000326671.

Chemistry databases

BindingDBiQ86VL8.
ChEMBLiCHEMBL1743127.
DrugBankiDB00787. Aciclovir.
GuidetoPHARMACOLOGYi1217.

Protein family/group databases

TCDBi2.A.66.1.20. the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily.

PTM databases

iPTMnetiQ86VL8.
PhosphoSitePlusiQ86VL8.

Polymorphism and mutation databases

BioMutaiSLC47A2.
DMDMi74727585.

Proteomic databases

PaxDbiQ86VL8.
PeptideAtlasiQ86VL8.
PRIDEiQ86VL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325411; ENSP00000326671; ENSG00000180638. [Q86VL8-1]
ENST00000350657; ENSP00000338084; ENSG00000180638. [Q86VL8-4]
ENST00000574239; ENSP00000458694; ENSG00000180638. [Q86VL8-6]
GeneIDi146802.
KEGGihsa:146802.
UCSCiuc002gwe.5. human. [Q86VL8-1]

Organism-specific databases

CTDi146802.
DisGeNETi146802.
GeneCardsiSLC47A2.
H-InvDBHIX0039367.
HGNCiHGNC:26439. SLC47A2.
MIMi609833. gene.
neXtProtiNX_Q86VL8.
OpenTargetsiENSG00000180638.
PharmGKBiPA162403847.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1347. Eukaryota.
COG0534. LUCA.
GeneTreeiENSGT00390000015713.
HOGENOMiHOG000060313.
HOVERGENiHBG056043.
InParanoidiQ86VL8.
KOiK03327.
OMAiIVACYHV.
OrthoDBiEOG091G06X2.
PhylomeDBiQ86VL8.
TreeFamiTF324441.

Enzyme and pathway databases

ReactomeiR-HSA-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

GeneWikiiSLC47A2.
GenomeRNAii146802.
PROiQ86VL8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000180638.
CleanExiHS_SLC47A2.
ExpressionAtlasiQ86VL8. baseline and differential.
GenevisibleiQ86VL8. HS.

Family and domain databases

InterProiIPR002528. MATE_fam.
[Graphical view]
PfamiPF01554. MatE. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00797. matE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS47A2_HUMAN
AccessioniPrimary (citable) accession number: Q86VL8
Secondary accession number(s): A0JBX9
, A0P8Z7, Q63HJ9, Q8IV44, Q96NA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.