Q86VI4Q3ZCV5Q7L909Q86VH8Q9H060LAP4B_HUMANLysosomal-associated transmembrane protein 4BLysosome-associated transmembrane protein 4-betaLAPTM4BPSEC0001Homo sapiensHumanEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomoMolecular cloning and characterization of LAPTM4B, a novel gene upregulated in hepatocellular carcinoma.NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)SUBCELLULAR LOCATIONNUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2)Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 31-317 (ISOFORM 1)LAPTMs regulate lysosomal function and interact with mucolipin 1: new clues for understanding mucolipidosis type IV.INTERACTION WITH MCOLN1SUBCELLULAR LOCATIONPROTEOLYTIC CLEAVAGEFUNCTIONA role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.SUBCELLULAR LOCATIONINTERACTION WITH NEDD4UBIQUITINATIONMUTAGENESIS OF PRO-296 AND PRO-312REGIONLAPTM4B is a PtdIns(4,5)P2 effector that regulates EGFR signaling, lysosomal sorting, and degradation.INTERACTION WITH PIP5K1C AND HGSSUBCELLULAR LOCATIONFUNCTIONMUTAGENESIS OF PRO-296 AND PRO-312Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.INTERACTION WITH LRRC32FUNCTIONLAPTM4B facilitates late endosomal ceramide export to control cell death pathways.FUNCTIONSUBCELLULAR LOCATIONLAPTM4b recruits the LAT1-4F2hc Leu transporter to lysosomes and promotes mTORC1 activation.INTERACTION WITH SLC3A2 AND SLC7A5FUNCTIONBeclin1 antagonizes LAPTM4B-mediated EGFR overactivation in gastric cancer cells.INTERACTION WITH BECN1 AND EGFRRequired for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).Homooligomer; upon reaching the lysosomes (PubMed:21224396). Interacts with MCOLN1 (PubMed:21224396). Interacts with NEDD4; may play a role in the lysosomal sorting of LAPTM4B; enhances HGS association with NEDD4; mediates inhibition of EGFR degradation (PubMed:22096579, PubMed:25588945). Interacts with PIP5K1C; promotes SNX5 association with LAPTM4B; kinase activity of PIP5K1C is required; interaction is regulated by phosphatidylinositol 4,5-bisphosphate generated by PIP5K1C (PubMed:25588945). Interacts with HGS; promotes HGS ubiquitination (PubMed:25588945). Interacts with SNX5 (PubMed:25588945). Interacts with SLC3A2 and SLC7A5; recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567). Interacts with LRRC32; decreases TGFB1 production in regulatory T cells (PubMed:26126825). Interacts with BECN1; competes with EGFR for LAPTM4B binding; regulates EGFR activity (PubMed:28479384). Interacts with EGFR; positively correlates with EGFR activation (PubMed:28479384).Q86VI4P08183false2Q86VI4Q99675false3Q86VI4Q4LDR2false3Q86VI4Q8NBI2false3Q86VI4P00533false10Q86VI4P54849false3Q86VI4Q96A65false2Q86VI4P48165false3Q86VI4Q8TED1false3Q86VI4P17181false3Q86VI4P24593false3Q86VI4Q6UXK2false3Q86VI4Q9H2K0false3Q86VI4Q58DX5false3Q86VI4Q02297-10false3Q86VI4Q2M2E3false3Q86VI4Q14108false3Q86VI4Q8N661false3Q86VI4A5PKU2false3Q86VI4Q96FB2false3Q86VI4-3P27986false2Endomembrane systemMulti-pass membrane proteinLate endosome membraneCell membraneCell projectionLysosome membraneEndosome membraneEndosomeMultivesicular body membraneEndosomeMultivesicular body lumenQ86VI4-31Q86VI4-22Undergoes proteolytic cleavage following delivery to the lysosomes.Ubiquitinated by NEDD4.Belongs to the LAPTM4/LAPTM5 transporter family.Probable cloning artifact.Alternative splicingCell membraneCell projectionEndosomeLysosomeMembraneReference proteomeTransmembraneTransmembrane helixTransportUbl conjugationPAPADRRSMTMTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA
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