Q86VI3 (IQGA3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating-like protein IQGAP3

Gene names

Name:

IQGAP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1631 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 CH (calponin-homology) domain. Contains 4 IQ domains. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calmodulin-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara G1/S transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara Ras protein signal transduction Inferred from electronic annotation. Source: Compara activation of MAPK activity Inferred from electronic annotation. Source: Compara cellular response to organic substance Inferred from electronic annotation. Source: Compara negative regulation of gene expression Inferred from electronic annotation. Source: Compara positive regulation of Ras GTPase activity Inferred from electronic annotation. Source: GOC positive regulation of gene expression Inferred from electronic annotation. Source: Compara positive regulation of mammary gland epithelial cell proliferation Inferred from electronic annotation. Source: Compara regulation of cell size Inferred from electronic annotation. Source: Compara
Cellular_component	cell-cell junction Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara lateral plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	Ras GTPase activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1631

1631

Ras GTPase-activating-like protein IQGAP3

PRO_0000056651

Regions

Domain

34 – 149

116

CH

Domain

730 – 759

30

IQ 1

Domain

760 – 789

30

IQ 2

Domain

790 – 819

30

IQ 3

Domain

820 – 849

30

IQ 4

Domain

988 – 1221

234

Ras-GAP

Amino acid modifications

Modified residue

539

1

Phosphoserine Ref.5 Ref.7

Modified residue

1424

1

Phosphoserine Ref.6 Ref.7

Natural variations

Natural variant

391

1

R → G. Ref.1 Ref.2
Corresponds to variant rs2488269 [ dbSNP | Ensembl ].

VAR_055278

Natural variant

410

1

V → L.
Corresponds to variant rs11264498 [ dbSNP | Ensembl ].

VAR_055279

Natural variant

645

1

D → N.
Corresponds to variant rs11264496 [ dbSNP | Ensembl ].

VAR_055280

Natural variant

663

1

R → C. Ref.1 Ref.2
Corresponds to variant rs744224 [ dbSNP | Ensembl ].

VAR_055281

Natural variant

770

1

H → Y.
Corresponds to variant rs1078890 [ dbSNP | Ensembl ].

VAR_055282

Natural variant

1061

1

Q → H.
Corresponds to variant rs11804414 [ dbSNP | Ensembl ].

VAR_055283

Natural variant

1097

1

P → S.
Corresponds to variant rs12562301 [ dbSNP | Ensembl ].

VAR_055284

Experimental info

Sequence conflict

610

1

A → S in BAC85501. Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

1631

Helix Strand Turn

Details...

Beta strand

1535 – 1538

4

Helix

1539 – 1544

6

Beta strand

1547 – 1551

5

Helix

1556 – 1561

6

Beta strand

1562 – 1568

7

Beta strand

1574 – 1581

8

Beta strand

1589 – 1592

4

Helix

1593 – 1601

9

Beta strand

1606 – 1609

4

Turn

1610 – 1612

3

Beta strand

1613 – 1616

4

Helix

1617 – 1627

11

Sequences

	Sequence		Length	Mass (Da)
	Q86VI3 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 93480B30076470DA	FASTA	1,631	184,699
	10 20 30 40 50 60 MERRAAGPGW AAYERLTAEE MDEQRRQNVA YQYLCRLEEA KRWMEACLKE ELPSPVELEE 70 80 90 100 110 120 SLRNGVLLAK LGHCFAPSVV PLKKIYDVEQ LRYQATGLHF RHTDNINFWL SAIAHIGLPS 130 140 150 160 170 180 TFFPETTDIY DKKNMPRVVY CIHALSLFLF RLGLAPQIHD LYGKVKFTAE ELSNMASELA 190 200 210 220 230 240 KYGLQLPAFS KIGGILANEL SVDEAAVHAA VLAINEAVER GVVEDTLAAL QNPSALLENL 250 260 270 280 290 300 REPLAAVYQE MLAQAKMEKA ANARNHDDRE SQDIYDHYLT QAEIQGNINH VNVHGALEVV 310 320 330 340 350 360 DDALERQSPE ALLKALQDPA LALRGVRRDF ADWYLEQLNS DREQKAQELG LVELLEKEEV 370 380 390 400 410 420 QAGVAAANTK GDQEQAMLHA VQRINKAIRR RVAADTVKEL MCPEAQLPPV YPVASSMYQL 430 440 450 460 470 480 ELAVLQQQQG ELGQEELFVA VEMLSAVVLI NRALEARDAS GFWSSLVNPA TGLAEVEGEN 490 500 510 520 530 540 AQRYFDALLK LRQERGMGED FLSWNDLQAT VSQVNAQTQE ETDRVLAVSL INEALDKGSP 550 560 570 580 590 600 EKTLSALLLP AAGLDDVSLP VAPRYHLLLV AAKRQKAQVT GDPGAVLWLE EIRQGVVRAN 610 620 630 640 650 660 QDTNTAQRMA LGVAAINQAI KEGKAAQTER VLRNPAVALR GVVPDCANGY QRALESAMAK 670 680 690 700 710 720 KQRPADTAFW VQHDMKDGTA YYFHLQTFQG IWEQPPGCPL NTSHLTREEI QSAVTKVTAA 730 740 750 760 770 780 YDRQQLWKAN VGFVIQLQAR LRGFLVRQKF AEHSHFLRTW LPAVIKIQAH WRGYRQRKIY 790 800 810 820 830 840 LEWLQYFKAN LDAIIKIQAW ARMWAARRQY LRRLHYFQKN VNSIVKIQAF FRARKAQDDY 850 860 870 880 890 900 RILVHAPHPP LSVVRRFAHL LNQSQQDFLA EAELLKLQEE VVRKIRSNQQ LEQDLNIMDI 910 920 930 940 950 960 KIGLLVKNRI TLQEVVSHCK KLTKRNKEQL SDMMVLDKQK GLKSLSKEKR QKLEAYQHLF 970 980 990 1000 1010 1020 YLLQTQPIYL AKLIFQMPQN KTTKFMEAVI FSLYNYASSR REAYLLLQLF KTALQEEIKS 1030 1040 1050 1060 1070 1080 KVEQPQDVVT GNPTVVRLVV RFYRNGRGQS ALQEILGKVI QDVLEDKVLS VHTDPVHLYK 1090 1100 1110 1120 1130 1140 NWINQTEAQT GQRSHLPYDV TPEQALSHPE VQRRLDIALR NLLAMTDKFL LAITSSVDQI 1150 1160 1170 1180 1190 1200 PYGMRYVAKV LKATLAEKFP DATDSEVYKV VGNLLYYRFL NPAVVAPDAF DIVAMAAGGA 1210 1220 1230 1240 1250 1260 LAAPQRHALG AVAQLLQHAA AGKAFSGQSQ HLRVLNDYLE ETHLKFRKFI HRACQVPEPE 1270 1280 1290 1300 1310 1320 ERFAVDEYSD MVAVAKPMVY ITVGELVNTH RLLLEHQDCI APDHQDPLHE LLEDLGELPT 1330 1340 1350 1360 1370 1380 IPDLIGESIA ADGHTDLSKL EVSLTLTNKF EGLEADADDS NTRSLLLSTK QLLADIIQFH 1390 1400 1410 1420 1430 1440 PGDTLKEILS LSASREQEAA HKQLMSRRQA CTAQTPEPLR RHRSLTAHSL LPLAEKQRRV 1450 1460 1470 1480 1490 1500 LRNLRRLEAL GLVSARNGYQ GLVDELAKDI RNQHRHRHRR KAELVKLQAT LQGLSTKTTF 1510 1520 1530 1540 1550 1560 YEEQGDYYSQ YIRACLDHLA PDSKSSGKGK KQPSLHYTAA QLLEKGVLVE IEDLPASHFR 1570 1580 1590 1600 1610 1620 NVIFDITPGD EAGKFEVNAK FLGVDMERFQ LHYQDLLQLQ YEGVAVMKLF NKAKVNVNLL 1630 IFLLNKKFLR K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Fukata M., Watanabe T., Noritake J., Kaibuchi K. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-391 AND CYS-663. Tissue: Embryonic kidney.
[2]	"Isolation of a novel human gene up-regulated in diffuse-type gastric cancer." Jinawath N., Furukawa Y., Nakamura Y. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-391 AND CYS-663.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 574-1631.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-539, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1424, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[7]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-539 AND SER-1424, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Crystal structure of the RGC domain of IQGAP3." Structural genomics consortium (SGC) Submitted (SEP-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) OF 1529-1631.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY253300 mRNA. Translation: AAP06954.1.
AB105103 mRNA. Translation: BAC78211.1.
AL365181 Genomic DNA. Translation: CAI13046.1.
AK122653 mRNA. Translation: BAC85501.1.

IPI

IPI00328905.

RefSeq

NP_839943.2. NM_178229.4.

UniGene

Hs.591495.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ISU	X-ray	1.88	A	1529-1631	[»]

ProteinModelPortal

Q86VI3.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q86VI3. 3 interactions.

STRING

9606.ENSP00000354451.

PTM databases

PhosphoSite

Q86VI3.

Polymorphism databases

DMDM

229462887.

Proteomic databases

PaxDb

Q86VI3.

PRIDE

Q86VI3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000361170; ENSP00000354451; ENSG00000183856.

GeneID

128239.

KEGG

hsa:128239.

UCSC

uc001fpf.3. human.

Organism-specific databases

CTD

128239.

GeneCards

GC01M156496.

H-InvDB

HIX0023560.

HGNC

HGNC:20669. IQGAP3.

HPA

HPA030142.
HPA030143.

neXtProt

NX_Q86VI3.

PharmGKB

PA134954177.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5261.

HOGENOM

HOG000004842.

HOVERGEN

HBG052143.

InParanoid

Q86VI3.

KO

K05767.

OMA

QRRQNVA.

OrthoDB

EOG4H19TW.

Gene expression databases

ArrayExpress

Q86VI3.

Bgee

Q86VI3.

CleanEx

HS_IQGAP3.

Genevestigator

Q86VI3.

GermOnline

ENSG00000183856. Homo sapiens.

Family and domain databases

Gene3D

1.10.418.10. 1 hit.
1.10.506.10. 2 hits.

InterPro

IPR001715. CH-domain.
IPR000048. IQ_motif_EF-hand-BS.
IPR001936. RasGAP.
IPR000593. RasGAP_C.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
[Graphical view]

Pfam

PF00307. CH. 1 hit.
PF00612. IQ. 4 hits.
PF00616. RasGAP. 1 hit.
PF03836. RasGAP_C. 1 hit.
[Graphical view]

SMART

SM00033. CH. 1 hit.
SM00015. IQ. 4 hits.
SM00323. RasGAP. 1 hit.
[Graphical view]

SUPFAM

SSF47576. Calponin-homology. 1 hit.
SSF48350. Rho_GAP. 1 hit.

PROSITE

PS50021. CH. 1 hit.
PS50096. IQ. 4 hits.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q86VI3.

GenomeRNAi

128239.

NextBio

82254.

Entry information

Entry name

IQGA3_HUMAN

Accession

Primary (citable) accession number: Q86VI3
Secondary accession number(s): Q5T3H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.