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Q86VF5 (MOGT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-acylglycerol O-acyltransferase 3

EC=2.3.1.20
EC=2.3.1.22
Alternative name(s):
Acyl-CoA:monoacylglycerol acyltransferase 3
Short name=MGAT3
Diacylglycerol O-acyltransferase candidate 7
Short name=hDC7
Diacylglycerol acyltransferase 2-like protein 7
Monoacylglycerol O-acyltransferase 3
Gene names
Name:MOGAT3
Synonyms:DC7, DGAT2L7
ORF Names:UNQ9383/PRO34208
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Also able to catalyze the terminal step in triacylglycerol synthesis by using diacylglycerol and fatty acyl-CoA as substrates. Has a preference toward palmitoyl-CoA and oleoyl-CoA. May be involved in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes. Ref.1

Catalytic activity

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. Ref.1

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. Ref.1

Pathway

Glycerolipid metabolism; triacylglycerol biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity Ref.1.

Tissue specificity

Selectively expressed in the digestive system. Highly expressed in the ileum, and at lower level in jejunum, duodenum, colon, cecum and the rectum. Not expressed in the stomach and the esophagus and trachea. Expressed at very low level in liver. Ref.1

Sequence similarities

Belongs to the diacylglycerol acyltransferase family.

Biophysicochemical properties

Kinetic parameters:

Vmax=9.3 nmol/min/mg enzyme with diacylglycerol as substrate Ref.1

Vmax=22.8 nmol/min/mg enzyme with 2-monoacylglycerol as substrate

Sequence caution

The sequence AAD45832.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86VF5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86VF5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     224-281: ASLVPVYSFG...LFSATSWGLL → GPPHPRPPAP...LHLGLAAAFR
     282-341: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q86VF5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     291-341: VGRPIPVPQR...VPASTCLTFI → GECPPPGGRP...RKSITITPST
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3413412-acylglycerol O-acyltransferase 3
PRO_0000249067

Regions

Transmembrane29 – 4921Helical; Potential
Transmembrane50 – 7021Helical; Potential
Transmembrane137 – 15721Helical; Potential

Amino acid modifications

Glycosylation1261N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence224 – 28158ASLVP…SWGLL → GPPHPRPPAPPPHRGGSQSL SRPLHDGPGAALRGAQGKLW GPRFHLPHLHLGLAAAFR in isoform 2.
VSP_020361
Alternative sequence282 – 34160Missing in isoform 2.
VSP_020362
Alternative sequence291 – 34151VGRPI…CLTFI → GECPPPGGRPPAAAWASGIP RPPVSLSLQWAAPSPSPSAS TPPRRKSITITPST in isoform 3.
VSP_020363

Experimental info

Sequence conflict2651C → Y in AAI00954. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 3F4981AE0D05B613

FASTA34138,730
        10         20         30         40         50         60 
MGVATTLQPP TTSKTLQKQH LEAVGAYQYV LTFLFMGPFF SLLVFVLLFT SLWPFSVFYL 

        70         80         90        100        110        120 
VWLYVDWDTP NQGGRRSEWI RNRAIWRQLR DYYPVKLVKT AELPPDRNYV LGAHPHGIMC 

       130        140        150        160        170        180 
TGFLCNFSTE SNGFSQLFPG LRPWLAVLAG LFYLPVYRDY IMSFGLCPVS RQSLDFILSQ 

       190        200        210        220        230        240 
PQLGQAVVIM VGGAHEALYS VPGEHCLTLQ KRKGFVRLAL RHGASLVPVY SFGENDIFRL 

       250        260        270        280        290        300 
KAFATGSWQH WCQLTFKKLM GFSPCIFWGR GLFSATSWGL LPFAVPITTV VGRPIPVPQR 

       310        320        330        340 
LHPTEEEVNH YHALYMTALE QLFEEHKESC GVPASTCLTF I 

« Hide

Isoform 2 [UniParc].

Checksum: D1D0C8B2EF0D3992
Show »

FASTA28131,498
Isoform 3 [UniParc].

Checksum: 3836BCF9E68C500C
Show »

FASTA34438,353

References

« Hide 'large scale' references
[1]"Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorption."
Cheng D., Nelson T.C., Chen J., Walker S.G., Wardwell-Swanson J., Meegalla R., Taub R., Billheimer J.T., Ramaker M., Feder J.N.
J. Biol. Chem. 278:13611-13614(2003) [PubMed: 12618427] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed: 12853948] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY229854 mRNA. Translation: AAO63579.1.
AY358200 mRNA. Translation: AAQ88567.1.
AC004876 Genomic DNA. Translation: AAD45832.1. Sequence problems.
BC100953 mRNA. Translation: AAI00954.1.
BC100954 mRNA. Translation: AAI00955.1.
BC100955 mRNA. Translation: AAI00956.1.
IPIIPI00328886.
IPI00645251.
IPI00784889.
RefSeqNP_835470.1. NM_178176.2.
UniGeneHs.512217.

3D structure databases

ProteinModelPortalQ86VF5.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ86VF5.

Polymorphism databases

DMDM74727570.

Proteomic databases

PRIDEQ86VF5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000223114; ENSP00000223114; ENSG00000106384.
GeneID346606.
KEGGhsa:346606.
UCSCuc003uyc.1. human.
uc010lhr.1. human.

Organism-specific databases

CTD346606.
GeneCardsGC07M100838.
H-InvDBHIX0201187.
HGNCHGNC:23249. MOGAT3.
HPAHPA011940.
MIM610184. gene.
neXtProtNX_Q86VF5.
PharmGKBPA134959238.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG12071.
GeneTreeENSGT00550000074276.
HOVERGENHBG065791.
InParanoidQ86VF5.
OMARRKSITI.
OrthoDBEOG408N8B.
PhylomeDBQ86VF5.

Enzyme and pathway databases

BRENDA2.3.1.22. 2681.

Gene expression databases

ArrayExpressQ86VF5.
BgeeQ86VF5.
CleanExHS_MOGAT3.
GenevestigatorQ86VF5.
GermOnlineENSG00000106384. Homo sapiens.

Family and domain databases

InterProIPR007130. DAGAT.
[Graphical view]
KOK14456.
PANTHERPTHR12317. DAGAT. 1 hit.
PfamPF03982. DAGAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio98991.
SOURCESearch...

Entry information

Entry nameMOGT3_HUMAN
AccessionPrimary (citable) accession number: Q86VF5
Secondary accession number(s): Q496A6 expand/collapse secondary AC list , Q496A7, Q496A8, Q9UDW7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families