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Protein

2-acylglycerol O-acyltransferase 3

Gene

MOGAT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Also able to catalyze the terminal step in triacylglycerol synthesis by using diacylglycerol and fatty acyl-CoA as substrates. Has a preference toward palmitoyl-CoA and oleoyl-CoA. May be involved in absorption of dietary fat in the small intestine by catalyzing the resynthesis of triacylglycerol in enterocytes.1 Publication

Catalytic activityi

Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol.1 Publication
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.1 Publication

Kineticsi

    1. Vmax=9.3 nmol/min/mg enzyme with diacylglycerol as substrate1 Publication
    2. Vmax=22.8 nmol/min/mg enzyme with 2-monoacylglycerol as substrate1 Publication

    Pathwayi: triacylglycerol biosynthesis

    This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
    View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciZFISH:HS12629-MONOMER.
    BRENDAi2.3.1.22. 2681.
    ReactomeiR-HSA-75109. Triglyceride Biosynthesis.
    UniPathwayiUPA00282.

    Chemistry databases

    SwissLipidsiSLP:000000307.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-acylglycerol O-acyltransferase 3 (EC:2.3.1.20, EC:2.3.1.22)
    Alternative name(s):
    Acyl-CoA:monoacylglycerol acyltransferase 3
    Short name:
    MGAT3
    Diacylglycerol O-acyltransferase candidate 7
    Short name:
    hDC7
    Diacylglycerol acyltransferase 2-like protein 7
    Monoacylglycerol O-acyltransferase 3
    Gene namesi
    Name:MOGAT3
    Synonyms:DC7, DGAT2L7
    ORF Names:UNQ9383/PRO34208
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:23249. MOGAT3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
    Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
    Transmembranei137 – 157HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi346606.
    OpenTargetsiENSG00000106384.
    PharmGKBiPA134959238.

    Polymorphism and mutation databases

    DMDMi74727570.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002490671 – 3412-acylglycerol O-acyltransferase 3Add BLAST341

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi126N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ86VF5.
    PRIDEiQ86VF5.

    Expressioni

    Tissue specificityi

    Selectively expressed in the digestive system. Highly expressed in the ileum, and at lower level in jejunum, duodenum, colon, cecum and the rectum. Not expressed in the stomach and the esophagus and trachea. Expressed at very low level in liver.1 Publication

    Gene expression databases

    BgeeiENSG00000106384.
    CleanExiHS_MOGAT3.
    GenevisibleiQ86VF5. HS.

    Organism-specific databases

    HPAiHPA011940.

    Interactioni

    Protein-protein interaction databases

    BioGridi131392. 1 interactor.
    STRINGi9606.ENSP00000223114.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86VF5.
    SMRiQ86VF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG0831. Eukaryota.
    ENOG410XTG3. LUCA.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ86VF5.
    KOiK14456.
    OMAiNQAIWRQ.
    OrthoDBiEOG091G0BGQ.
    PhylomeDBiQ86VF5.
    TreeFamiTF314707.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: Q86VF5-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MGVATTLQPP TTSKTLQKQH LEAVGAYQYV LTFLFMGPFF SLLVFVLLFT
    60 70 80 90 100
    SLWPFSVFYL VWLYVDWDTP NQGGRRSEWI RNRAIWRQLR DYYPVKLVKT
    110 120 130 140 150
    AELPPDRNYV LGAHPHGIMC TGFLCNFSTE SNGFSQLFPG LRPWLAVLAG
    160 170 180 190 200
    LFYLPVYRDY IMSFGLCPVS RQSLDFILSQ PQLGQAVVIM VGGAHEALYS
    210 220 230 240 250
    VPGEHCLTLQ KRKGFVRLAL RHGASLVPVY SFGENDIFRL KAFATGSWQH
    260 270 280 290 300
    WCQLTFKKLM GFSPCIFWGR GLFSATSWGL LPFAVPITTV VGRPIPVPQR
    310 320 330 340
    LHPTEEEVNH YHALYMTALE QLFEEHKESC GVPASTCLTF I
    Length:341
    Mass (Da):38,730
    Last modified:June 1, 2003 - v1
    Checksum:i3F4981AE0D05B613
    GO
    Isoform 2 (identifier: Q86VF5-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         224-281: ASLVPVYSFG...LFSATSWGLL → GPPHPRPPAP...LHLGLAAAFR
         282-341: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:281
    Mass (Da):31,498
    Checksum:iD1D0C8B2EF0D3992
    GO
    Isoform 3 (identifier: Q86VF5-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         291-341: VGRPIPVPQR...VPASTCLTFI → GECPPPGGRP...RKSITITPST

    Note: No experimental confirmation available.
    Show »
    Length:344
    Mass (Da):38,353
    Checksum:i3836BCF9E68C500C
    GO

    Sequence cautioni

    The sequence AAD45832 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti265C → Y in AAI00954 (PubMed:15489334).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_020361224 – 281ASLVP…SWGLL → GPPHPRPPAPPPHRGGSQSL SRPLHDGPGAALRGAQGKLW GPRFHLPHLHLGLAAAFR in isoform 2. 1 PublicationAdd BLAST58
    Alternative sequenceiVSP_020362282 – 341Missing in isoform 2. 1 PublicationAdd BLAST60
    Alternative sequenceiVSP_020363291 – 341VGRPI…CLTFI → GECPPPGGRPPAAAWASGIP RPPVSLSLQWAAPSPSPSAS TPPRRKSITITPST in isoform 3. 1 PublicationAdd BLAST51

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY229854 mRNA. Translation: AAO63579.1.
    AY358200 mRNA. Translation: AAQ88567.1.
    AC004876 Genomic DNA. Translation: AAD45832.1. Sequence problems.
    BC100953 mRNA. Translation: AAI00954.1.
    BC100954 mRNA. Translation: AAI00955.1.
    BC100955 mRNA. Translation: AAI00956.1.
    CCDSiCCDS5714.1. [Q86VF5-1]
    CCDS75643.1. [Q86VF5-2]
    RefSeqiNP_001274076.1. NM_001287147.1. [Q86VF5-2]
    NP_835470.1. NM_178176.3. [Q86VF5-1]
    XP_005250366.1. XM_005250309.3. [Q86VF5-3]
    UniGeneiHs.512217.

    Genome annotation databases

    EnsembliENST00000223114; ENSP00000223114; ENSG00000106384. [Q86VF5-1]
    ENST00000379423; ENSP00000368734; ENSG00000106384. [Q86VF5-2]
    ENST00000440203; ENSP00000403756; ENSG00000106384. [Q86VF5-3]
    GeneIDi346606.
    KEGGihsa:346606.
    UCSCiuc003uyc.5. human. [Q86VF5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY229854 mRNA. Translation: AAO63579.1.
    AY358200 mRNA. Translation: AAQ88567.1.
    AC004876 Genomic DNA. Translation: AAD45832.1. Sequence problems.
    BC100953 mRNA. Translation: AAI00954.1.
    BC100954 mRNA. Translation: AAI00955.1.
    BC100955 mRNA. Translation: AAI00956.1.
    CCDSiCCDS5714.1. [Q86VF5-1]
    CCDS75643.1. [Q86VF5-2]
    RefSeqiNP_001274076.1. NM_001287147.1. [Q86VF5-2]
    NP_835470.1. NM_178176.3. [Q86VF5-1]
    XP_005250366.1. XM_005250309.3. [Q86VF5-3]
    UniGeneiHs.512217.

    3D structure databases

    ProteinModelPortaliQ86VF5.
    SMRiQ86VF5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi131392. 1 interactor.
    STRINGi9606.ENSP00000223114.

    Chemistry databases

    SwissLipidsiSLP:000000307.

    Polymorphism and mutation databases

    DMDMi74727570.

    Proteomic databases

    PaxDbiQ86VF5.
    PRIDEiQ86VF5.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000223114; ENSP00000223114; ENSG00000106384. [Q86VF5-1]
    ENST00000379423; ENSP00000368734; ENSG00000106384. [Q86VF5-2]
    ENST00000440203; ENSP00000403756; ENSG00000106384. [Q86VF5-3]
    GeneIDi346606.
    KEGGihsa:346606.
    UCSCiuc003uyc.5. human. [Q86VF5-1]

    Organism-specific databases

    CTDi346606.
    DisGeNETi346606.
    GeneCardsiMOGAT3.
    HGNCiHGNC:23249. MOGAT3.
    HPAiHPA011940.
    MIMi610184. gene.
    neXtProtiNX_Q86VF5.
    OpenTargetsiENSG00000106384.
    PharmGKBiPA134959238.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0831. Eukaryota.
    ENOG410XTG3. LUCA.
    GeneTreeiENSGT00750000117391.
    HOGENOMiHOG000179738.
    HOVERGENiHBG065791.
    InParanoidiQ86VF5.
    KOiK14456.
    OMAiNQAIWRQ.
    OrthoDBiEOG091G0BGQ.
    PhylomeDBiQ86VF5.
    TreeFamiTF314707.

    Enzyme and pathway databases

    UniPathwayiUPA00282.
    BioCyciZFISH:HS12629-MONOMER.
    BRENDAi2.3.1.22. 2681.
    ReactomeiR-HSA-75109. Triglyceride Biosynthesis.

    Miscellaneous databases

    GenomeRNAii346606.
    PROiQ86VF5.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000106384.
    CleanExiHS_MOGAT3.
    GenevisibleiQ86VF5. HS.

    Family and domain databases

    InterProiIPR007130. DAGAT.
    [Graphical view]
    PfamiPF03982. DAGAT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMOGT3_HUMAN
    AccessioniPrimary (citable) accession number: Q86VF5
    Secondary accession number(s): Q496A6
    , Q496A7, Q496A8, Q9UDW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: June 1, 2003
    Last modified: November 2, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.