Q86V88 (MGDP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium-dependent phosphatase 1
Short name=MDP-1
EC=3.1.3.-
EC=3.1.3.48

Gene names

Name:

MDP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Magnesium-dependent phosphatase which may act as a tyrosine phosphatase By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by vanadate and zinc, and slightly by calcium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86V88-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86V88-2)

The sequence of this isoform differs from the canonical sequence as follows:
     107-127: LQQKTGIPFSQMIFFDDERRN → YAEIREEQGEKVSERPGKPRY
     128-176: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q86V88-3)

The sequence of this isoform differs from the canonical sequence as follows:
     89-122: YFVHREIYPGSKITHFERLQQKTGIPFSQMIFFD → CYLHSHPEWNESSNSKSRVRDICEGPNWAFEVQP
     123-176: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 176

176

Magnesium-dependent phosphatase 1

PRO_0000068827

Sites

Active site

11

1

Nucleophile By similarity

Active site

13

1

Proton donor By similarity

Metal binding

11

1

Magnesium By similarity

Metal binding

13

1

Magnesium By similarity

Metal binding

123

1

Magnesium By similarity

Binding site

12

1

Phosphate; via amide nitrogen By similarity

Binding site

13

1

Phosphate; via amide nitrogen By similarity

Binding site

20

1

Substrate By similarity

Binding site

69

1

Phosphate By similarity

Binding site

70

1

Phosphate By similarity

Binding site

70

1

Substrate By similarity

Binding site

100

1

Phosphate By similarity

Natural variations

Alternative sequence

89 – 122

34

YFVHR…MIFFD → CYLHSHPEWNESSNSKSRVR DICEGPNWAFEVQP in isoform 3.

VSP_015985

Alternative sequence

107 – 127

21

LQQKT…DERRN → YAEIREEQGEKVSERPGKPR Y in isoform 2.

VSP_015986

Alternative sequence

123 – 176

54

Missing in isoform 3.

VSP_015988

Alternative sequence

128 – 176

49

Missing in isoform 2.

VSP_015987

Secondary structure

1

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176

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Turn

13 – 15

3

Beta strand

16 – 19

4

Turn

21 – 23

3

Helix

51 – 61

11

Beta strand

65 – 69

5

Helix

74 – 83

10

Turn

87 – 89

3

Beta strand

90 – 98

9

Helix

100 – 111

12

Helix

115 – 117

3

Beta strand

118 – 123

6

Helix

125 – 132

8

Turn

133 – 135

3

Beta strand

137 – 140

4

Beta strand

142 – 144

3

Helix

147 – 159

13

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B0A33BD02458B2EF	FASTA	176	20,109
	10 20 30 40 50 60 MARLPKLAVF DLDYTLWPFW VDTHVDPPFH KSSDGTVRDR RGQDVRLYPE VPEVLKRLQS 70 80 90 100 110 120 LGVPGAAASR TSEIEGANQL LELFDLFRYF VHREIYPGSK ITHFERLQQK TGIPFSQMIF 130 140 150 160 170 FDDERRNIVD VSKLGVTCIH IQNGMNLQTL SQGLETFAKA QTGPLRSSLE ESPFEA
	Isoform 2 [UniParc]. Checksum: 3203C1FE34BB9CA2	FASTA	127	14,818
	10 20 30 40 50 60 MARLPKLAVF DLDYTLWPFW VDTHVDPPFH KSSDGTVRDR RGQDVRLYPE VPEVLKRLQS 70 80 90 100 110 120 LGVPGAAASR TSEIEGANQL LELFDLFRYF VHREIYPGSK ITHFERYAEI REEQGEKVSE RPGKPRY
	Isoform 3 [UniParc]. Checksum: B016158C63765443	FASTA	122	14,038
	10 20 30 40 50 60 MARLPKLAVF DLDYTLWPFW VDTHVDPPFH KSSDGTVRDR RGQDVRLYPE VPEVLKRLQS 70 80 90 100 110 120 LGVPGAAASR TSEIEGANQL LELFDLFRCY LHSHPEWNES SNSKSRVRDI CEGPNWAFEV QP

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Small intestine.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Brain and Liver.
[3]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK092821 mRNA. Translation: BAC03984.1.
BC046912 mRNA. Translation: AAH46912.1.
BC051382 mRNA. Translation: AAH51382.1.

IPI

IPI00337556.
IPI00384628.
IPI00385758.

RefSeq

NP_001186750.1. NM_001199821.1.
NP_612485.2. NM_138476.3.

UniGene

Hs.220963.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WM8	X-ray	1.75	A	1-165	[»]

ProteinModelPortal

Q86V88.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-1464579.

STRING

9606.ENSP00000288087.

PTM databases

PhosphoSite

Q86V88.

Polymorphism databases

DMDM

74727544.

Proteomic databases

PaxDb

Q86V88.

PRIDE

Q86V88.

Protocols and materials databases

DNASU

145553.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000288087; ENSP00000288087; ENSG00000213920.
ENST00000396833; ENSP00000380045; ENSG00000213920.

GeneID

145553.

KEGG

hsa:145553.

UCSC

uc001wnk.2. human.
uc001wnl.2. human.
uc001wnm.2. human.

Organism-specific databases

CTD

145553.

GeneCards

GC14M024683.

HGNC

HGNC:28781. MDP1.

HPA

HPA003064.

neXtProt

NX_Q86V88.

PharmGKB

PA165479165.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG279690.

HOGENOM

HOG000216653.

HOVERGEN

HBG081971.

InParanoid

Q86V88.

OMA

NGMSLQT.

OrthoDB

EOG432107.

Gene expression databases

ArrayExpress

Q86V88.

Bgee

Q86V88.

Genevestigator

Q86V88.

GermOnline

ENSG00000100931. Homo sapiens.

Family and domain databases

Gene3D

3.40.50.1000. 1 hit.

InterPro

IPR023214. HAD-like_dom.
IPR010033. HAD_SF_ppase_IIIC.
IPR024734. MDP_1_eu.
IPR010036. MDP_1_eu_arc.
[Graphical view]

PANTHER

PTHR17901. PTHR17901. 1 hit.

Pfam

PF12689. Acid_PPase. 1 hit.
[Graphical view]

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR01681. HAD-SF-IIIC. 1 hit.
TIGR01685. MDP-1. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q86V88.

GenomeRNAi

145553.

NextBio

85134.

Entry information

Entry name

MGDP1_HUMAN

Accession

Primary (citable) accession number: Q86V88
Secondary accession number(s): Q86Y84, Q8NAD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.