PIM3

Functionⁱ

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.5 Publications

Catalytic activityⁱ

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	69	ATPPROSITE-ProRule annotation		1
Active siteⁱ	170	Proton acceptorPROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	46 – 54	ATPPROSITE-ProRule annotation		9

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
protein serine/threonine kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 18593906

Complete GO annotation...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
cell cycle Source: UniProtKB-KW
negative regulation of apoptotic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17270021
negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
protein autophosphorylation Source: GO_Central
protein phosphorylation
Source: UniProtKB
Inferred from direct assayⁱ
- 18593906
regulation of mitotic cell cycle
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 17270021

Complete GO annotation...

Keywordsⁱ

Molecular function	Kinase, Serine/threonine-protein kinase, Transferase
Biological process	Apoptosis, Cell cycle
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkⁱ	Q86V86.
SIGNORⁱ	Q86V86.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Serine/threonine-protein kinase pim-3 (EC:2.7.11.1)
Gene namesⁱ	Name:PIM3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 22

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:19310. PIM3.

HGNCⁱ

HGNC:19310. PIM3.

Subcellular locationⁱ

Cytoplasm
1 Publication
Manual assertion based on experiment inⁱ
- Ref.10
  "Aberrant Pim-3 expression is involved in gastric adenoma-adenocarcinoma sequence and cancer progression."
  Zheng H.C., Tsuneyama K., Takahashi H., Miwa S., Sugiyama T., Popivanova B.K., Fujii C., Nomoto K., Mukaida N., Takano Y.
  J. Cancer Res. Clin. Oncol. 134:481-488(2008) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, FUNCTION.

GO - Cellular componentⁱ

cytoplasm Source: GO_Central
cytosol Source: HPA

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

Proto-oncogene

Organism-specific databases

DisGeNET More...DisGeNETⁱ	415116.
Open Targets More...OpenTargetsⁱ	ENSG00000198355.
PharmGKBⁱ	PA134980758.

Chemistry databases

ChEMBLⁱ	CHEMBL5407.
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2160.

Polymorphism and mutation databases

BioMutaⁱ	PIM3.
DMDMⁱ	215274221.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000086533	1 – 326	Serine/threonine-protein kinase pim-3Add BLAST		326

Post-translational modificationⁱ

Ubiquitinated, leading to proteasomal degradation.1 Publication

Phosphorylated. Interaction with PPP2CA promotes dephosphorylation.1 Publication

Keywords - PTMⁱ

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q86V86.
PaxDbⁱ	Q86V86.
PeptideAtlas More...PeptideAtlasⁱ	Q86V86.
PRIDEⁱ	Q86V86.

PTM databases

iPTMnetⁱ	Q86V86.
PhosphoSitePlusⁱ	Q86V86.

Expressionⁱ

Tissue specificityⁱ

Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.3 Publications

Gene expression databases

Bgeeⁱ	ENSG00000198355.
CleanExⁱ	HS_PIM3.
Genevisibleⁱ	Q86V86. HS.

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA068758.

HPAⁱ

HPA068758.

Interactionⁱ

Subunit structureⁱ

Interacts with BAD. Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation. Interacts with SOCS6 (By similarity).By similarity

Protein-protein interaction databases

BioGridⁱ	136104. 1 interactor.
IntActⁱ	Q86V86. 2 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-6823599.
STRINGⁱ	9606.ENSP00000353824.

Chemistry databases

BindingDBⁱ

Q86V86.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q86V86.
SMRⁱ	Q86V86.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	40 – 293	Protein kinasePROSITE-ProRule annotationAdd BLAST		254

Sequence similaritiesⁱ

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0583. Eukaryota. COG0515. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119045.
HOGENOMⁱ	HOG000231357.
HOVERGENⁱ	HBG106681.
InParanoidⁱ	Q86V86.
KEGG Orthology (KO) More...KOⁱ	K08807.
OMAⁱ	MDHLPVK.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0IMW.
PhylomeDBⁱ	Q86V86.
TreeFamⁱ	TF320810.

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009. Kinase-like_dom. IPR017348. PIM1/2/3. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069. Pkinase. 1 hit.
PIRSFⁱ	PIRSF037993. STPK_Pim-1. 1 hit.
SMARTⁱ	View protein in SMART SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q86V86-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG 
        60         70         80         90        100
FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA 
       110        120        130        140        150
GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF 
       160        170        180        190        200
FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV 
       210        220        230        240        250
YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE 
       260        270        280        290        300
ILRGRLLFRR RVSPECQQLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGV 
       310        320 
PESCDLRLCT LDPDDVASTT SSSESL

Length:326

Mass (Da):35,891

Last modified:November 25, 2008 - v3

Checksum:ⁱ41FDEFAC4367A162

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	37	E → K in AAI41856 (PubMed:15489334).Curated	1
Sequence conflictⁱ	300	V → A in BAD42438 (PubMed:15540201).Curated	1
Sequence conflictⁱ	300	V → A in BAF84694 (PubMed:16141072).Curated	1
Sequence conflictⁱ	300	V → A in AAI41856 (PubMed:15489334).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB114795 mRNA. Translation: BAD42438.1. AK292005 mRNA. Translation: BAF84694.1. CR536608, BX539320 Genomic DNA. Translation: CAO03523.1. BX539320, CR536608 Genomic DNA. Translation: CAO03581.1. BC052239 mRNA. No translation available. BC141855 mRNA. Translation: AAI41856.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33678.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001001852.2. NM_001001852.3.
UniGeneⁱ	Hs.530381.

Genome annotation databases

Ensemblⁱ	ENST00000360612; ENSP00000353824; ENSG00000198355.
GeneIDⁱ	415116.
KEGGⁱ	hsa:415116.
UCSC genome browser More...UCSCⁱ	uc003bjb.4. human.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB114795 mRNA. Translation: BAD42438.1. AK292005 mRNA. Translation: BAF84694.1. CR536608, BX539320 Genomic DNA. Translation: CAO03523.1. BX539320, CR536608 Genomic DNA. Translation: CAO03581.1. BC052239 mRNA. No translation available. BC141855 mRNA. Translation: AAI41856.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS33678.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_001001852.2. NM_001001852.3.
UniGeneⁱ	Hs.530381.

3D structure databases

ProteinModelPortalⁱ	Q86V86.
SMRⁱ	Q86V86.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	136104. 1 interactor.
IntActⁱ	Q86V86. 2 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-6823599.
STRINGⁱ	9606.ENSP00000353824.

Chemistry databases

BindingDBⁱ	Q86V86.
ChEMBLⁱ	CHEMBL5407.
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2160.

PTM databases

iPTMnetⁱ	Q86V86.
PhosphoSitePlusⁱ	Q86V86.

Polymorphism and mutation databases

BioMutaⁱ	PIM3.
DMDMⁱ	215274221.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q86V86.
PaxDbⁱ	Q86V86.
PeptideAtlas More...PeptideAtlasⁱ	Q86V86.
PRIDEⁱ	Q86V86.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000360612; ENSP00000353824; ENSG00000198355.
GeneIDⁱ	415116.
KEGGⁱ	hsa:415116.
UCSC genome browser More...UCSCⁱ	uc003bjb.4. human.

Organism-specific databases

CTDⁱ	415116.
DisGeNET More...DisGeNETⁱ	415116.
GeneCardsⁱ	PIM3.
H-InvDBⁱ	HIX0203204.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:19310. PIM3.
Human Protein Atlas More...HPAⁱ	HPA068758.
MIMⁱ	610580. gene.
neXtProtⁱ	NX_Q86V86.
Open Targets More...OpenTargetsⁱ	ENSG00000198355.
PharmGKBⁱ	PA134980758.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0583. Eukaryota. COG0515. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119045.
HOGENOMⁱ	HOG000231357.
HOVERGENⁱ	HBG106681.
InParanoidⁱ	Q86V86.
KEGG Orthology (KO) More...KOⁱ	K08807.
OMAⁱ	MDHLPVK.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0IMW.
PhylomeDBⁱ	Q86V86.
TreeFamⁱ	TF320810.

Enzyme and pathway databases

SignaLinkⁱ	Q86V86.
SIGNORⁱ	Q86V86.

Miscellaneous databases

ChiTaRSⁱ	PIM3. human.
GenomeRNAiⁱ	415116.
Protein Ontology More...PROⁱ	PR:Q86V86.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000198355.
CleanExⁱ	HS_PIM3.
Genevisibleⁱ	Q86V86. HS.

Family and domain databases

InterProⁱ	View protein in InterPro IPR011009. Kinase-like_dom. IPR017348. PIM1/2/3. IPR000719. Prot_kinase_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00069. Pkinase. 1 hit.
PIRSFⁱ	PIRSF037993. STPK_Pim-1. 1 hit.
SMARTⁱ	View protein in SMART SM00220. S_TKc. 1 hit.
SUPFAMⁱ	SSF56112. SSF56112. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PIM3_HUMAN
Accessionⁱ	Q86V86Primary (citable) accession number: Q86V86 Secondary accession number(s): A5D8X8 , A8K7J0, B1B0P0, Q68BM2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 21, 2003
	Last sequence update:	November 25, 2008
	Last modified:	June 7, 2017
	This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human and mouse protein kinases
Human and mouse protein kinases: classification and index
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q86V86 (PIM3_HUMAN)

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Serine/threonine-protein kinase pim-3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ