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Protein

5'-nucleotidase domain-containing protein 3

Gene

NT5DC3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei102 – 1021NucleophileBy similarity
Metal bindingi102 – 1021MagnesiumBy similarity
Active sitei104 – 1041Proton donorBy similarity
Metal bindingi104 – 1041Magnesium; via carbonyl oxygenBy similarity
Metal bindingi389 – 3891MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
5'-nucleotidase domain-containing protein 3 (EC:3.1.3.-)
Alternative name(s):
GRP94-neighboring nucleotidase
Gene namesi
Name:NT5DC3
Synonyms:GNN, TU12B1-TY
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:30826. NT5DC3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • mitochondrion Source: Ensembl
  • receptor complex Source: MGI
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485562.

Polymorphism and mutation databases

BioMutaiNT5DC3.
DMDMi74727508.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5485485'-nucleotidase domain-containing protein 3PRO_0000326247Add
BLAST

Proteomic databases

EPDiQ86UY8.
MaxQBiQ86UY8.
PaxDbiQ86UY8.
PeptideAtlasiQ86UY8.
PRIDEiQ86UY8.

PTM databases

DEPODiQ86UY8.
iPTMnetiQ86UY8.
PhosphoSiteiQ86UY8.

Expressioni

Tissue specificityi

Expressed in brain, placenta, skeletal muscle, pancreas, testis, uterus, and small intestine. Reduced expression in pancreatic cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000111696.
CleanExiHS_NT5DC3.
ExpressionAtlasiQ86UY8. baseline and differential.
GenevisibleiQ86UY8. HS.

Organism-specific databases

HPAiCAB025217.

Interactioni

Protein-protein interaction databases

BioGridi119609. 6 interactions.
IntActiQ86UY8. 5 interactions.
STRINGi9606.ENSP00000376615.

Structurei

3D structure databases

ProteinModelPortaliQ86UY8.
SMRiQ86UY8. Positions 85-526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni251 – 2599Substrate bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 74Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2470. Eukaryota.
ENOG410XT8W. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000230610.
HOVERGENiHBG053334.
OMAiDFKMSAN.
OrthoDBiEOG091G048T.
PhylomeDBiQ86UY8.
TreeFamiTF323990.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UY8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMAAAAVVA RGAGARAATA AALRGGCGTA ARGRPCAGPA RPLCTAPGTA
60 70 80 90 100
PDMKRYLWER YREAKRSTEE LVPSIMSNLL NPDAIFSNNE MSLSDIEIYG
110 120 130 140 150
FDYDYTLVFY SKHLHTLIFN AARDLLINEH RYPAEIRKYE YDPNFAIRGL
160 170 180 190 200
HYDVQRAVLM KIDAFHYIQL GTVYRGLSVV PDEEVIEMYE GSHVPLEQMS
210 220 230 240 250
DFYGKSSHGN TMKQFMDIFS LPEMTLLSCV NEYFLKNNID YEPVHLYKDV
260 270 280 290 300
KDSIRDVHIK GIMYRAIEAD IEKYICYAEQ TRAVLAKLAD HGKKMFLITN
310 320 330 340 350
SPSSFVDKGM SYIVGKDWRD LFDVVIVQAE KPNFFNDKRR PFRKMNEKGV
360 370 380 390 400
LLWDKIHKLQ KGQIYKQGNL YEFLKLTGWR GSRVLYFGDH IYSDLADLTL
410 420 430 440 450
KHGWRTGAII PELRSELKIM NTEQYIQTMT WLQTLTGLLE QMQVHRDAES
460 470 480 490 500
QLVLQEWKKE RKEMREMTKS FFNAQFGSLF RTDQNPTYFL RRLSRFADIY
510 520 530 540
MASLSCLLNY DVSHTFYPRR TPLQHELPAW SERPPTFGTP LLQEAQAK
Length:548
Mass (Da):63,420
Last modified:June 1, 2003 - v1
Checksum:i93B1C8CD7C2E0434
GO
Isoform 2 (identifier: Q86UY8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     466-474: Missing.

Show »
Length:464
Mass (Da):54,665
Checksum:iEB11B4E3E4C9D69E
GO

Sequence cautioni

The sequence BAA92095 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA93053 differs from that shown. Reason: Frameshift at position 62. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti266 – 2661A → E.
Corresponds to variant rs12184494 [ dbSNP | Ensembl ].
VAR_040043

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7575Missing in isoform 2. CuratedVSP_032643Add
BLAST
Alternative sequencei466 – 4749Missing in isoform 2. CuratedVSP_032644

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032773 mRNA. Translation: BAA93053.1. Frameshift.
AB032786 Genomic DNA. Translation: BAA93086.1.
CH471054 Genomic DNA. Translation: EAW97716.1.
BC052293 mRNA. Translation: AAH52293.1.
AK002128 mRNA. Translation: BAA92095.1. Different initiation.
CCDSiCCDS41824.1. [Q86UY8-1]
RefSeqiNP_001026871.1. NM_001031701.2. [Q86UY8-1]
UniGeneiHs.48428.

Genome annotation databases

EnsembliENST00000392876; ENSP00000376615; ENSG00000111696. [Q86UY8-1]
GeneIDi51559.
KEGGihsa:51559.
UCSCiuc010swe.2. human. [Q86UY8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032773 mRNA. Translation: BAA93053.1. Frameshift.
AB032786 Genomic DNA. Translation: BAA93086.1.
CH471054 Genomic DNA. Translation: EAW97716.1.
BC052293 mRNA. Translation: AAH52293.1.
AK002128 mRNA. Translation: BAA92095.1. Different initiation.
CCDSiCCDS41824.1. [Q86UY8-1]
RefSeqiNP_001026871.1. NM_001031701.2. [Q86UY8-1]
UniGeneiHs.48428.

3D structure databases

ProteinModelPortaliQ86UY8.
SMRiQ86UY8. Positions 85-526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119609. 6 interactions.
IntActiQ86UY8. 5 interactions.
STRINGi9606.ENSP00000376615.

PTM databases

DEPODiQ86UY8.
iPTMnetiQ86UY8.
PhosphoSiteiQ86UY8.

Polymorphism and mutation databases

BioMutaiNT5DC3.
DMDMi74727508.

Proteomic databases

EPDiQ86UY8.
MaxQBiQ86UY8.
PaxDbiQ86UY8.
PeptideAtlasiQ86UY8.
PRIDEiQ86UY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392876; ENSP00000376615; ENSG00000111696. [Q86UY8-1]
GeneIDi51559.
KEGGihsa:51559.
UCSCiuc010swe.2. human. [Q86UY8-1]

Organism-specific databases

CTDi51559.
GeneCardsiNT5DC3.
HGNCiHGNC:30826. NT5DC3.
HPAiCAB025217.
MIMi611076. gene.
neXtProtiNX_Q86UY8.
PharmGKBiPA143485562.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2470. Eukaryota.
ENOG410XT8W. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000230610.
HOVERGENiHBG053334.
OMAiDFKMSAN.
OrthoDBiEOG091G048T.
PhylomeDBiQ86UY8.
TreeFamiTF323990.

Miscellaneous databases

GenomeRNAii51559.
PROiQ86UY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111696.
CleanExiHS_NT5DC3.
ExpressionAtlasiQ86UY8. baseline and differential.
GenevisibleiQ86UY8. HS.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNT5D3_HUMAN
AccessioniPrimary (citable) accession number: Q86UY8
Secondary accession number(s): Q9NUM7, Q9P2T2, Q9P2T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.