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Protein

Protein FAM83A

Gene

FAM83A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable proto-oncogene that functions in the epidermal growth factor receptor/EGFR signaling pathway. Activates both RAS/MAPK and PI3K/AKT/TOR signaling cascades downstream of EGFR. Required for the RAS/MAPK signaling cascade activation upon EGFR stimulation, it also activates both signaling cascades independently of EGFR activation.1 Publication

GO - Molecular functioni

  • phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • epidermal growth factor receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein FAM83ACurated
Alternative name(s):
Tumor antigen BJ-TSA-91 Publication
Tumor-specific gene expressed in prostate protein1 Publication
Gene namesi
Name:FAM83AImported
Synonyms:TSGP1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:28210. FAM83A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

Organism-specific databases

PharmGKBiPA142671851.

Polymorphism and mutation databases

BioMutaiFAM83A.
DMDMi74727505.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Protein FAM83APRO_0000286813Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei348 – 3481PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated upon EGFR activation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ86UY5.
MaxQBiQ86UY5.
PaxDbiQ86UY5.
PRIDEiQ86UY5.

PTM databases

iPTMnetiQ86UY5.
PhosphoSiteiQ86UY5.

Expressioni

Gene expression databases

BgeeiQ86UY5.
CleanExiHS_FAM83A.
GenevisibleiQ86UY5. HS.

Organism-specific databases

HPAiHPA052615.
HPA062538.

Interactioni

Subunit structurei

Interacts with the regulatory subunit p85 of PI3-kinase; increased by EGFR activation (PubMed:22886303). Interacts with RAF1; increased by EGFR activation it activates RAF1 (PubMed:22886303, PubMed:24736947).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIM23P364063EBI-1384254,EBI-740098
TRIM27P143733EBI-1384254,EBI-719493

GO - Molecular functioni

  • phosphatidylinositol 3-kinase regulatory subunit binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi124411. 8 interactions.
IntActiQ86UY5. 6 interactions.

Structurei

Secondary structure

1
434
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi128 – 1303Combined sources
Beta strandi135 – 1395Combined sources
Beta strandi142 – 1443Combined sources
Helixi146 – 15611Combined sources
Beta strandi160 – 1678Combined sources
Helixi172 – 18312Combined sources
Beta strandi188 – 1947Combined sources
Helixi195 – 1973Combined sources
Helixi198 – 20811Combined sources
Helixi213 – 2153Combined sources
Beta strandi216 – 2238Combined sources
Beta strandi227 – 2293Combined sources
Beta strandi235 – 2373Combined sources
Beta strandi244 – 2474Combined sources
Turni248 – 2503Combined sources
Beta strandi251 – 2566Combined sources
Turni261 – 2666Combined sources
Beta strandi270 – 2756Combined sources
Helixi278 – 29114Combined sources
Beta strandi294 – 2985Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4URJX-ray2.68A/B/C/D122-304[»]
ProteinModelPortaliQ86UY5.
SMRiQ86UY5. Positions 134-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi320 – 35738Ser-richAdd
BLAST
Compositional biasi355 – 39440Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the FAM83 family.Curated

Phylogenomic databases

eggNOGiENOG410IGUW. Eukaryota.
ENOG410XSTH. LUCA.
GeneTreeiENSGT00760000119168.
HOGENOMiHOG000112483.
HOVERGENiHBG107905.
InParanoidiQ86UY5.
OMAiTWRPFLQ.
OrthoDBiEOG7G7KNX.
PhylomeDBiQ86UY5.
TreeFamiTF330777.

Family and domain databases

InterProiIPR012461. DUF1669.
[Graphical view]
PANTHERiPTHR16181. PTHR16181. 1 hit.
PfamiPF07894. DUF1669. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UY5-1) [UniParc]FASTAAdd to basket

Also known as: TSGP-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRSRHLGKI RKRLEDVKSQ WVRPARADFS DNESARLATD ALLDGGSEAY
60 70 80 90 100
WRVLSQEGEV DFLSSVEAQY IQAQAREPPC PPDTLGGAEA GPKGLDSSSL
110 120 130 140 150
QSGTYFPVAS EGSEPALLHS WASAEKPYLK EKSSATVYFQ TVKHNNIRDL
160 170 180 190 200
VRRCITRTSQ VLVILMDVFT DVEIFCDILE AANKRGVFVC VLLDQGGVKL
210 220 230 240 250
FQEMCDKVQI SDSHLKNISI RSVEGEIYCA KSGRKFAGQI REKFIISDWR
260 270 280 290 300
FVLSGSYSFT WLCGHVHRNI LSKFTGQAVE LFDEEFRHLY ASSKPVMGLK
310 320 330 340 350
SPRLVAPVPP GAAPANGRLS SSSGSASDRT SSNPFSGRSA GSHPGTRSVS
360 370 380 390 400
ASSGPCSPAA PHPPPPPRFQ PHQGPWGAPS PQAHLSPRPH DGPPAAVYSN
410 420 430
LGAYRPTRLQ LEQLGLVPRL TPTWRPFLQA SPHF
Length:434
Mass (Da):47,458
Last modified:June 1, 2003 - v1
Checksum:i63D09D927D452FCC
GO
Isoform 2 (identifier: Q86UY5-2) [UniParc]FASTAAdd to basket

Also known as: TSGP-S

The sequence of this isoform differs from the canonical sequence as follows:
     161-216: Missing.
     348-350: SVS → TDG
     351-434: Missing.

Show »
Length:294
Mass (Da):32,158
Checksum:i73D90BAEC4F5B8E3
GO
Isoform 3 (identifier: Q86UY5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-367: GTRSVSASSGPCSPAAPHPPPPP → VPESKQNKTKTKKQTTLWFLMAF
     368-434: Missing.

Show »
Length:367
Mass (Da):40,576
Checksum:i849DCF2B4F8A681C
GO

Sequence cautioni

The sequence BAC05299.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti237 – 2371A → T.3 Publications
Corresponds to variant rs7813708 [ dbSNP | Ensembl ].
VAR_032178

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei161 – 21656Missing in isoform 2. 2 PublicationsVSP_025187Add
BLAST
Alternative sequencei345 – 36723GTRSV…PPPPP → VPESKQNKTKTKKQTTLWFL MAF in isoform 3. 1 PublicationVSP_025188Add
BLAST
Alternative sequencei348 – 3503SVS → TDG in isoform 2. 2 PublicationsVSP_025190
Alternative sequencei351 – 43484Missing in isoform 2. 2 PublicationsVSP_025191Add
BLAST
Alternative sequencei368 – 43467Missing in isoform 3. 1 PublicationVSP_025189Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ280322 mRNA. Translation: ABB91800.1.
DQ280323 mRNA. Translation: ABB91801.1.
AF497803 mRNA. Translation: AAQ07260.1.
BC007828 mRNA. Translation: AAH07828.1.
BC052300 mRNA. Translation: AAH52300.1.
AK098407 mRNA. Translation: BAC05299.1. Different initiation.
CCDSiCCDS6339.1. [Q86UY5-3]
CCDS6340.1. [Q86UY5-1]
CCDS75784.1. [Q86UY5-2]
RefSeqiNP_001275516.1. NM_001288587.1. [Q86UY5-2]
NP_116288.2. NM_032899.5. [Q86UY5-1]
NP_996889.1. NM_207006.2. [Q86UY5-3]
UniGeneiHs.379821.
Hs.679530.

Genome annotation databases

EnsembliENST00000276699; ENSP00000276699; ENSG00000147689. [Q86UY5-3]
ENST00000518448; ENSP00000428876; ENSG00000147689. [Q86UY5-1]
ENST00000522648; ENSP00000427979; ENSG00000147689. [Q86UY5-2]
ENST00000536633; ENSP00000445218; ENSG00000147689. [Q86UY5-3]
GeneIDi84985.
KEGGihsa:84985.
UCSCiuc003ypv.5. human. [Q86UY5-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ280322 mRNA. Translation: ABB91800.1.
DQ280323 mRNA. Translation: ABB91801.1.
AF497803 mRNA. Translation: AAQ07260.1.
BC007828 mRNA. Translation: AAH07828.1.
BC052300 mRNA. Translation: AAH52300.1.
AK098407 mRNA. Translation: BAC05299.1. Different initiation.
CCDSiCCDS6339.1. [Q86UY5-3]
CCDS6340.1. [Q86UY5-1]
CCDS75784.1. [Q86UY5-2]
RefSeqiNP_001275516.1. NM_001288587.1. [Q86UY5-2]
NP_116288.2. NM_032899.5. [Q86UY5-1]
NP_996889.1. NM_207006.2. [Q86UY5-3]
UniGeneiHs.379821.
Hs.679530.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4URJX-ray2.68A/B/C/D122-304[»]
ProteinModelPortaliQ86UY5.
SMRiQ86UY5. Positions 134-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124411. 8 interactions.
IntActiQ86UY5. 6 interactions.

PTM databases

iPTMnetiQ86UY5.
PhosphoSiteiQ86UY5.

Polymorphism and mutation databases

BioMutaiFAM83A.
DMDMi74727505.

Proteomic databases

EPDiQ86UY5.
MaxQBiQ86UY5.
PaxDbiQ86UY5.
PRIDEiQ86UY5.

Protocols and materials databases

DNASUi84985.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000276699; ENSP00000276699; ENSG00000147689. [Q86UY5-3]
ENST00000518448; ENSP00000428876; ENSG00000147689. [Q86UY5-1]
ENST00000522648; ENSP00000427979; ENSG00000147689. [Q86UY5-2]
ENST00000536633; ENSP00000445218; ENSG00000147689. [Q86UY5-3]
GeneIDi84985.
KEGGihsa:84985.
UCSCiuc003ypv.5. human. [Q86UY5-1]

Organism-specific databases

CTDi84985.
GeneCardsiFAM83A.
H-InvDBHIX0007762.
HGNCiHGNC:28210. FAM83A.
HPAiHPA052615.
HPA062538.
neXtProtiNX_Q86UY5.
PharmGKBiPA142671851.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IGUW. Eukaryota.
ENOG410XSTH. LUCA.
GeneTreeiENSGT00760000119168.
HOGENOMiHOG000112483.
HOVERGENiHBG107905.
InParanoidiQ86UY5.
OMAiTWRPFLQ.
OrthoDBiEOG7G7KNX.
PhylomeDBiQ86UY5.
TreeFamiTF330777.

Miscellaneous databases

ChiTaRSiFAM83A. human.
GeneWikiiFAM83A.
GenomeRNAii84985.
NextBioi75554.
PROiQ86UY5.

Gene expression databases

BgeeiQ86UY5.
CleanExiHS_FAM83A.
GenevisibleiQ86UY5. HS.

Family and domain databases

InterProiIPR012461. DUF1669.
[Graphical view]
PANTHERiPTHR16181. PTHR16181. 1 hit.
PfamiPF07894. DUF1669. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TSGP, a shared tumor specific gene expressed in prostate cancer."
    Yang D., Nelson C.C., Li D., Gleave M.E.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
  2. "BJ-TSA-9, a tumor specific gene which was characterized from lung carcinoma and colon carcinoma."
    Xueyuan D., Weifeng C.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT THR-237.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-237.
    Tissue: Lung and Pancreas.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 134-434 (ISOFORM 1), VARIANT THR-237.
    Tissue: Thyroid.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-348, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "FAM83A confers EGFR-TKI resistance in breast cancer cells and in mice."
    Lee S.Y., Meier R., Furuta S., Lenburg M.E., Kenny P.A., Xu R., Bissell M.J.
    J. Clin. Invest. 122:3211-3220(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH PI3-KINASE AND RAF1, SUBCELLULAR LOCATION.
  7. "Conserved oncogenic behavior of the FAM83 family regulates MAPK signaling in human cancer."
    Cipriano R., Miskimen K.L., Bryson B.L., Foy C.R., Bartel C.A., Jackson M.W.
    Mol. Cancer Res. 12:1156-1165(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAF1.
  8. "Crystal structure of human Bj-Tsa-9."
    Pinkas D.M., Sanvitale C., Wang D., Krojer T., Kopec J., Chaikuad A., Dixon Clarke S., Berridge G., Burgess-Brown N., Von Delft F., Arrowsmith C., Edwards A., Bountra C., Bullock A.
    Submitted (JUN-2014) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS) OF 122-304.

Entry informationi

Entry nameiFA83A_HUMAN
AccessioniPrimary (citable) accession number: Q86UY5
Secondary accession number(s): Q71HL2, Q8N7I1, Q96I47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.