Reviewed, UniProtKB/Swiss-Prot Q86UX7 (URP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Fermitin family homolog 3
Alternative name(s):
    Unc-112-related protein 2
    Kindlin-3
    MIG2-like protein

Gene names

Name:	FERMT3
Synonyms:	KIND3, MIG2B, URP2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	667 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in cell adhesion By similarity.
Subcellular location	Cytoplasm Probable. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Membrane-associated in chronic lymphocytic leukemia (CLL) cells.
Tissue specificity	Highly expressed in lymph node. Expressed in thymus, spleen and leukocytes. Weakly expressed in placenta, small intestine, stomach, testis and lung. Overexpressed in B-cell malignancies. Ref.1 Ref.4
Domain	The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Sequence similarities	Belongs to the kindlin family. Contains 1 FERM domain. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EXOSC10	Q01780	1	EBI-373158,EBI-358236
LSM8	O95777	1	EBI-373158,EBI-347779

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86UX7-1)

Also known as: Long; URP2LF;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86UX7-2)

Also known as: Short; URP2SF;

The sequence of this isoform differs from the canonical sequence as follows:
     360-363: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 667	667	Fermitin family homolog 3		PRO_0000219454
Regions
	Domain	229 – 558	330	FERM
	Domain	354 – 457	104	PH
	Compositional bias	147 – 155	9	Poly-Lys
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.6
	Modified residue	11	1	Phosphotyrosine Ref.6
	Modified residue	504	1	Phosphotyrosine Ref.5
Natural variations
	Alternative sequence	360 – 363	4	Missing in isoform 2.		VSP_009226
Experimental info
	Sequence conflict	472	1	A → P in BAC04220. Ref.2
	Sequence conflict	480	1	Q → R in BAC04220. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) (URP2LF) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5ACE15EB689B91B5	FASTA	667	75,953
	10 20 30 40 50 60 MAGMKTASGD YIDSSWELRV FVGEEDPEAE SVTLRVTGES HIGGVLLKIV EQINRKQDWS 70 80 90 100 110 120 DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRAL RLRASFSQPL 130 140 150 160 170 180 FQAVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE LYDLSKVVLA GGVAPALFRG 190 200 210 220 230 240 MPAHFSDSAQ TEACYHMLSR PQPPPDPLLL QRLPRPSSLS DKTQLHSRWL DSSRCLMQQG 250 260 270 280 290 300 IKAGDALWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL LEEIDCTEEE MMVFAALQYH 310 320 330 340 350 360 INKLSQSGEV GEPAGTDPGL DDLDVALSNL EVKLEGSAPT DVLDSLTTIP ELKDHLRIFR 370 380 390 400 410 420 IPRRPRKLTL KGYRQHWVVF KETTLSYYKS QDEAPGDPIQ QLNLKGCEVV PDVNVSGQKF 430 440 450 460 470 480 CIKLLVPSPE GMSEIYLRCQ DEQQYARWMA GCRLASKGRT MADSSYTSEV QAILAFLSLQ 490 500 510 520 530 540 RTGSGGPGNH PHGPDASAEG LNPYGLVAPR FQRKFKAKQL TPRILEAHQN VAQLSLAEAQ 550 560 570 580 590 600 LRFIQAWQSL PDFGISYVMV RFKGSRKDEI LGIANNRLIR IDLAVGDVVK TWRFSNMRQW 610 620 630 640 650 660 NVNWDIRQVA IEFDEHINVA FSCVSASCRI VHEYIGGYIF LSTRERARGE ELDEDLFLQL TGGHEAF
	Isoform 2 (Short) (URP2SF). Checksum: 243CA27636C67079	FASTA	663	75,430
	10 20 30 40 50 60 MAGMKTASGD YIDSSWELRV FVGEEDPEAE SVTLRVTGES HIGGVLLKIV EQINRKQDWS 70 80 90 100 110 120 DHAIWWEQKR QWLLQTHWTL DKYGILADAR LFFGPQHRPV ILRLPNRRAL RLRASFSQPL 130 140 150 160 170 180 FQAVAAICRL LSIRHPEELS LLRAPEKKEK KKKEKEPEEE LYDLSKVVLA GGVAPALFRG 190 200 210 220 230 240 MPAHFSDSAQ TEACYHMLSR PQPPPDPLLL QRLPRPSSLS DKTQLHSRWL DSSRCLMQQG 250 260 270 280 290 300 IKAGDALWLR FKYYSFFDLD PKTDPVRLTQ LYEQARWDLL LEEIDCTEEE MMVFAALQYH 310 320 330 340 350 360 INKLSQSGEV GEPAGTDPGL DDLDVALSNL EVKLEGSAPT DVLDSLTTIP ELKDHLRIFR 370 380 390 400 410 420 PRKLTLKGYR QHWVVFKETT LSYYKSQDEA PGDPIQQLNL KGCEVVPDVN VSGQKFCIKL 430 440 450 460 470 480 LVPSPEGMSE IYLRCQDEQQ YARWMAGCRL ASKGRTMADS SYTSEVQAIL AFLSLQRTGS 490 500 510 520 530 540 GGPGNHPHGP DASAEGLNPY GLVAPRFQRK FKAKQLTPRI LEAHQNVAQL SLAEAQLRFI 550 560 570 580 590 600 QAWQSLPDFG ISYVMVRFKG SRKDEILGIA NNRLIRIDLA VGDVVKTWRF SNMRQWNVNW 610 620 630 640 650 660 DIRQVAIEFD EHINVAFSCV SASCRIVHEY IGGYIFLSTR ERARGEELDE DLFLQLTGGH EAF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"URP1: a member of a novel family of PH and FERM domain-containing membrane-associated proteins is significantly over-expressed in lung and colon carcinomas." Weinstein E.J., Bourner M., Head R., Zakeri H., Bauer C., Mazzarella R. Biochim. Biophys. Acta 1637:207-216(2003) [PubMed: 12697302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Lung and Lymph.
[4]	"Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B." Boyd R.S., Adam P.J., Patel S., Loader J.A., Berry J., Redpath N.T., Poyser H.R., Fletcher G.C., Burgess N.A., Stamps A.C., Hudson L., Smith P., Griffiths M., Willis T.G., Karran E.L., Oscier D.G., Catovsky D., Terrett J.A., Dyer M.J.S. Leukemia 17:1605-1612(2003) [PubMed: 12886250] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-35; 424-438 AND 569-577, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-504, MASS SPECTROMETRY.
[6]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8 AND TYR-11, MASS SPECTROMETRY. Tissue: Platelet.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY093951 mRNA. Translation: AAM19736.1.
AY093952 mRNA. Translation: AAM19737.1.
AK093719 mRNA. Translation: BAC04220.1.
BC004347 mRNA. Translation: AAH04347.2.
BC013366 mRNA. Translation: AAH13366.1.
BC015584 mRNA. Translation: AAH15584.1.

IPI

IPI00216699.
IPI00397834.

RefSeq

NP_113659.3.
NP_848537.1.

UniGene

Hs.180535

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YS3	NMR	-	A	349-478	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q86UX7. 2 interactions.

PTM databases

PhosphoSite

Q86UX7.

Proteomic databases

PRIDE

Q86UX7.

Genome annotation databases

Ensembl

ENSG00000149781. Homo sapiens. [Contig view]

GeneID

83706.

KEGG

hsa:83706.

UCSC

uc001nyl.1. human.
uc001nym.1. human.

Organism-specific databases

GeneCards

GC11P063731.

H-InvDB

HIX0022682.

HGNC

HGNC:23151. FERMT3.

MIM

607901. gene.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

Q86UX7.

HOVERGEN

Q86UX7.

OMA

Q86UX7. RIDLAVG.

Gene expression databases

ArrayExpress

Q86UX7.

Bgee

Q86UX7.

CleanEx

HS_FERMT3.

GermOnline

ENSG00000149781. Homo sapiens.

Family and domain databases

InterPro

IPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 2 hits.

Pfam

PF00373. FERM_M. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]

SMART

SM00295. B41. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]

PROSITE

PS00660. FERM_1. False negative.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. False negative.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

72692.

PMAP-CutDB

Q86UX7.

SOURCE

Search...

Entry information

Entry name

URP2_HUMAN

Accession

Primary (citable) accession number: Q86UX7
Secondary accession number(s): Q8IUA1, Q8N207, Q9BT48

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	July 7, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)