Q86UX6 (ST32C_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase 32C
EC=2.7.11.1

Alternative name(s):
PKE
Yet another novel kinase 3

Gene names

Name:	STK32C
Synonyms:	YANK3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q60592
Cofactor	Magnesium By similarity. UniProtKB Q60592
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.5 (identifier: Q86UX6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q86UX6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-117: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Serine/threonine-protein kinase 32C		PRO_0000232415
Regions
	Domain	93 – 353	261	Protein kinase
	Nucleotide binding	99 – 107	9	ATP By similarity UniProtKB Q60592
Sites
	Active site	216	1	Proton acceptor By similarity UniProtKB Q60592
	Binding site	122	1	ATP By similarity UniProtKB Q60592
Amino acid modifications
	Modified residue	15	1	Phosphoserine Ref.5
	Modified residue	18	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 117	117	Missing in isoform 2. Ref.5		VSP_051998
	Natural variant	334	1	T → A. Ref.3 Ref.4 Corresponds to variant rs17854384 [ dbSNP | Ensembl ].		VAR_025900
	Natural variant	376	1	R → H in a colorectal cancer sample; somatic mutation. Ref.6		VAR_035637
	Natural variant	454	1	A → T. Ref.7 Corresponds to variant rs56109103 [ dbSNP | Ensembl ].		VAR_041170
	Natural variant	467	1	E → K. Ref.7 Corresponds to variant rs55812591 [ dbSNP | Ensembl ].		VAR_041171

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 38FEFBB3863B21F3	FASTA	486	54,994
	10 20 30 40 50 60 MRSGAERRGS SAAASPGSPP PGRARPAGSD APSALPPPAA GQPRARDSGD VRSQPRPLFQ 70 80 90 100 110 120 WSKWKKRMGS SMSAATARRP VFDDKEDVNF DHFQILRAIG KGSFGKVCIV QKRDTEKMYA 130 140 150 160 170 180 MKYMNKQQCI ERDEVRNVFR ELEILQEIEH VFLVNLWYSF QDEEDMFMVV DLLLGGDLRY 190 200 210 220 230 240 HLQQNVQFSE DTVRLYICEM ALALDYLRGQ HIIHRDVKPD NILLDERGHA HLTDFNIATI 250 260 270 280 290 300 IKDGERATAL AGTKPYMAPE IFHSFVNGGT GYSFEVDWWS VGVMAYELLR GWRPYDIHSS 310 320 330 340 350 360 NAVESLVQLF STVSVQYVPT WSKEMVALLR KLLTVNPEHR LSSLQDVQAA PALAGVLWDH 370 380 390 400 410 420 LSEKRVEPGF VPNKGRLHCD PTFELEEMIL ESRPLHKKKK RLAKNKSRDN SRDSSQSEND 430 440 450 460 470 480 YLQDCLDAIQ QDFVIFNREK LKRSQDLPRE PLPAPESRDA AEPVEDEAER SALPMCGPIC PSAGSG
	Isoform 2 [UniParc]. Checksum: 83C54C4AF7D792E5	FASTA	369	42,425
	10 20 30 40 50 60 MYAMKYMNKQ QCIERDEVRN VFRELEILQE IEHVFLVNLW YSFQDEEDMF MVVDLLLGGD 70 80 90 100 110 120 LRYHLQQNVQ FSEDTVRLYI CEMALALDYL RGQHIIHRDV KPDNILLDER GHAHLTDFNI 130 140 150 160 170 180 ATIIKDGERA TALAGTKPYM APEIFHSFVN GGTGYSFEVD WWSVGVMAYE LLRGWRPYDI 190 200 210 220 230 240 HSSNAVESLV QLFSTVSVQY VPTWSKEMVA LLRKLLTVNP EHRLSSLQDV QAAPALAGVL 250 260 270 280 290 300 WDHLSEKRVE PGFVPNKGRL HCDPTFELEE MILESRPLHK KKKRLAKNKS RDNSRDSSQS 310 320 330 340 350 360 ENDYLQDCLD AIQQDFVIFN REKLKRSQDL PREPLPAPES RDAAEPVEDE AERSALPMCG PICPSAGSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PKE, a new human AGC group kinase, phosphorylates SET, a PP2A inhibitor." Ma A.-H., Nelson D.A., Xia L., Ravi L., Chen H.-C., Robinson D.R., Kung H.-J. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT ALA-334. Tissue: Testis.
[4]	"The protein kinase complement of the human genome." Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S. Science 298:1912-1934(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15 AND SER-18, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-376.
[7]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] THR-454 AND LYS-467.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY098866 mRNA. Translation: AAM21719.1. AL512622, AL590105 Genomic DNA. Translation: CAI12181.1. AL512622 Genomic DNA. Translation: CAI12187.1. AL590105, AL512622 Genomic DNA. Translation: CAI13011.1. BC045760 mRNA. Translation: AAH45760.1.
IPI	IPI00328835. IPI00645556.
RefSeq	NP_775846.2. NM_173575.2.
UniGene	Hs.469002.
3D structure databases
ProteinModelPortal	Q86UX6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86UX6. 2 interactions.
STRING	9606.ENSP00000298630.
PTM databases
PhosphoSite	Q86UX6.
Polymorphism databases
DMDM	74762451.
Proteomic databases
PaxDb	Q86UX6.
PRIDE	Q86UX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000298630; ENSP00000298630; ENSG00000165752. ENST00000368622; ENSP00000357611; ENSG00000165752.
GeneID	282974.
KEGG	hsa:282974.
UCSC	uc001llb.2. human. uc001lld.1. human.
Organism-specific databases
CTD	282974.
GeneCards	GC10M134020.
HGNC	HGNC:21332. STK32C.
HPA	HPA017894.
neXtProt	NX_Q86UX6.
PharmGKB	PA134888050.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000021314.
HOVERGEN	HBG106458.
InParanoid	Q86UX6.
KO	K08793.
PhylomeDB	Q86UX6.
Gene expression databases
ArrayExpress	Q86UX6.
Bgee	Q86UX6.
CleanEx	HS_STK32C.
Genevestigator	Q86UX6.
GermOnline	ENSG00000165752. Homo sapiens.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q86UX6.
ChEMBL	CHEMBL5405.
GenomeRNAi	282974.
NextBio	93549.

Entry information

Entry name

ST32C_HUMAN

Accession

Primary (citable) accession number: Q86UX6
Secondary accession number(s): Q5T0Q5, Q86UE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.