Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q86UW9 (DTX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable E3 ubiquitin-protein ligase DTX2

EC=6.3.2.-
Alternative name(s):
Protein deltex-2
Short name=Deltex2
Short name=hDTX2
RING finger protein 58
Gene names
Name:DTX2
Synonyms:KIAA1528, RNF58
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 By similarity. Ref.1

Subcellular location

Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Partially nuclear. Ref.6

Domain

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases By similarity.

Sequence similarities

Belongs to the Deltex family.

Contains 1 RING-type zinc finger.

Contains 2 WWE domains.

Sequence caution

The sequence BAA96052.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processNotch signaling pathway
Ubl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainRepeat
Zinc-finger
   LigandMetal-binding
Zinc
   Molecular functionLigase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nuclear membrane

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay. Source: HPA

   Molecular_functionligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

UBE2D4Q9Y2X83EBI-740376,EBI-745527

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86UW9-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86UW9-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     337-383: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Probable E3 ubiquitin-protein ligase DTX2
PRO_0000219083

Regions

Domain8 – 9790WWE 1
Domain98 – 17477WWE 2
Zinc finger412 – 47362RING-type

Amino acid modifications

Modified residue2491N6-acetyllysine Ref.8
Modified residue3601Phosphoserine Ref.7

Natural variations

Alternative sequence337 – 38347Missing in isoform 2.
VSP_008350
Natural variant941A → T.
Corresponds to variant rs2462312 [ dbSNP | Ensembl ].
VAR_016920
Natural variant3841G → E. Ref.1 Ref.2 Ref.5
Corresponds to variant rs1638152 [ dbSNP | Ensembl ].
VAR_016921
Natural variant4211T → A. Ref.2 Ref.3
Corresponds to variant rs6979487 [ dbSNP | Ensembl ].
VAR_016922

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (A) [UniParc].

Last modified June 21, 2005. Version 3.
Checksum: ABE1398204F2273A

FASTA62267,246
        10         20         30         40         50         60 
MAMAPSPSLV QVYTSPAAVA VWEWQDGLGT WHPYSATVCS FIEQQFVQQK GQRFGLGSLA 

        70         80         90        100        110        120 
HSIPLGQADP SLAPYIIDLP SWTQFRQDTG TMRAVRRHLF PQHSAPGRGV VWEWLSDDGS 

       130        140        150        160        170        180 
WTAYEASVCD YLEQQVARGN QLVDLAPLGY NYTVNYTTHT QTNKTSSFCR SVRRQAGPPY 

       190        200        210        220        230        240 
PVTTIIAPPG HTGVACSCHQ CLSGSRTGPV SGRYRHSMTN LPAYPVPQHP PHRTASVFGT 

       250        260        270        280        290        300 
HQAFAPYNKP SLSGARSAPR LNTTNAWGAA PPSLGSQPLY RSSLSHLGPQ HLPPGSSTSG 

       310        320        330        340        350        360 
AVSASLPSGP SSSPGSVPAT VPMQMPKPSR VQQALAGMTS VLMSAIGLPV CLSRAPQPTS 

       370        380        390        400        410        420 
PPASRLASKS HGSVKRLRKM SVKGATPKPE PEPEQVIKNY TEELKVPPDE DCIICMEKLS 

       430        440        450        460        470        480 
TASGYSDVTD SKAIGSLAVG HLTKCSHAFH LLCLLAMYCN GNKDGSLQCP SCKTIYGEKT 

       490        500        510        520        530        540 
GTQPQGKMEV LRFQMSLPGH EDCGTILIVY SIPHGIQGPE HPNPGKPFTA RGFPRQCYLP 

       550        560        570        580        590        600 
DNAQGRKVLE LLKVAWKRRL IFTVGTSSTT GETDTVVWNE IHHKTEMDRN ITGHGYPDPN 

       610        620 
YLQNVLAELA AQGVTEDCLE QQ 

« Hide

Isoform 2 (B) [UniParc].

Checksum: 449202FB53C026B3
Show »

FASTA57562,344

References

« Hide 'large scale' references
[1]"The BAL-binding protein BBAP and related Deltex family members exhibit ubiquitin-protein isopeptide ligase activity."
Takeyama K., Aguiar R.C.T., Gu L., He C., Freeman G.J., Kutok J.L., Aster J.C., Shipp M.A.
J. Biol. Chem. 278:21930-21937(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), VARIANT GLU-384, SUBUNIT, IN VITRO UBIQUITIN LIGASE ACTIVITY.
[2]"Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.
DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS GLU-384 AND ALA-421.
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-421.
Tissue: Thyroid.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLU-384.
Tissue: Brain, Lymph and Muscle.
[6]"Role of Deltex-1 as a transcriptional regulator downstream of the Notch receptor."
Yamamoto N., Yamamoto S., Inagaki F., Kawaichi M., Fukamizu A., Kishi N., Matsuno K., Nakamura K., Weinmaster G., Okano H., Nakafuku M.
J. Biol. Chem. 276:45031-45040(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-360, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY225124 mRNA. Translation: AAP57518.1.
AY225125 mRNA. Translation: AAP57519.1.
AB040961 mRNA. Translation: BAA96052.1. Different initiation.
AK023924 mRNA. Translation: BAB14727.1.
AC005522 Genomic DNA. Translation: AAP21881.1.
AC007078 Genomic DNA. No translation available.
BC008856 mRNA. Translation: AAH08856.1.
BC018555 mRNA. Translation: AAH18555.1.
BC026059 mRNA. Translation: AAH26059.1.
BC093079 mRNA. Translation: AAH93079.1.
RefSeqNP_001096064.1. NM_001102594.1.
NP_001096065.1. NM_001102595.1.
NP_001096066.1. NM_001102596.1.
NP_065943.2. NM_020892.2.
XP_005250188.1. XM_005250131.1.
XP_005250189.1. XM_005250132.1.
XP_005250191.1. XM_005250134.1.
UniGeneHs.187058.

3D structure databases

ProteinModelPortalQ86UW9.
SMRQ86UW9. Positions 18-182, 392-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125266. 15 interactions.
IntActQ86UW9. 18 interactions.
MINTMINT-1436106.
STRING9606.ENSP00000322885.

PTM databases

PhosphoSiteQ86UW9.

Polymorphism databases

DMDM68067880.

Proteomic databases

PaxDbQ86UW9.
PRIDEQ86UW9.

Protocols and materials databases

DNASU113878.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000307569; ENSP00000305242; ENSG00000091073. [Q86UW9-2]
ENST00000324432; ENSP00000322885; ENSG00000091073. [Q86UW9-1]
ENST00000413936; ENSP00000390218; ENSG00000091073. [Q86UW9-1]
ENST00000430490; ENSP00000411986; ENSG00000091073. [Q86UW9-1]
ENST00000446820; ENSP00000392545; ENSG00000091073. [Q86UW9-2]
GeneID113878.
KEGGhsa:113878.
UCSCuc003uff.4. human. [Q86UW9-1]
uc003ufj.4. human. [Q86UW9-2]

Organism-specific databases

CTD113878.
GeneCardsGC07P076090.
HGNCHGNC:15973. DTX2.
HPAHPA042931.
MIM613141. gene.
neXtProtNX_Q86UW9.
PharmGKBPA27515.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0323.
HOGENOMHOG000007352.
HOVERGENHBG007213.
InParanoidQ86UW9.
KOK06058.
OMAQPQGKME.
OrthoDBEOG7J17ZB.
PhylomeDBQ86UW9.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_116125. Disease.
UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ86UW9.
BgeeQ86UW9.
CleanExHS_DTX2.
GenevestigatorQ86UW9.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
[Graphical view]
PROSITEPS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDTX2. human.
GeneWikiDTX2.
GenomeRNAi113878.
NextBio78930.
PROQ86UW9.
SOURCESearch...

Entry information

Entry nameDTX2_HUMAN
AccessionPrimary (citable) accession number: Q86UW9
Secondary accession number(s): Q6XM87 expand/collapse secondary AC list , Q6XM88, Q96H69, Q9H890, Q9P200
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM