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Protein

Probable E3 ubiquitin-protein ligase DTX2

Gene

DTX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Functions as a ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri412 – 473RING-typePROSITE-ProRule annotationAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processNotch signaling pathway, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase DTX2 (EC:2.3.2.27By similarity)
Alternative name(s):
Protein deltex-2
Short name:
Deltex2
Short name:
hDTX2
RING finger protein 58
RING-type E3 ubiquitin transferase DTX2Curated
Gene namesi
Name:DTX2
Synonyms:KIAA1528, RNF58
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:15973. DTX2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi113878.
OpenTargetsiENSG00000091073.
PharmGKBiPA27515.

Polymorphism and mutation databases

BioMutaiDTX2.
DMDMi68067880.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190831 – 622Probable E3 ubiquitin-protein ligase DTX2Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei213Asymmetric dimethylarginineCombined sources1
Modified residuei215Asymmetric dimethylarginineCombined sources1
Modified residuei233Asymmetric dimethylarginineCombined sources1
Modified residuei249N6-acetyllysineCombined sources1
Modified residuei256Omega-N-methylarginineCombined sources1
Modified residuei360PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ86UW9.
MaxQBiQ86UW9.
PaxDbiQ86UW9.
PeptideAtlasiQ86UW9.
PRIDEiQ86UW9.

PTM databases

iPTMnetiQ86UW9.
PhosphoSitePlusiQ86UW9.

Expressioni

Gene expression databases

BgeeiENSG00000091073.
CleanExiHS_DTX2.
ExpressionAtlasiQ86UW9. baseline and differential.
GenevisibleiQ86UW9. HS.

Organism-specific databases

HPAiHPA042931.

Interactioni

Subunit structurei

Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1.By similarity

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi125266. 42 interactors.
IntActiQ86UW9. 94 interactors.
MINTiMINT-1436106.
STRINGi9606.ENSP00000322885.

Structurei

3D structure databases

ProteinModelPortaliQ86UW9.
SMRiQ86UW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 97WWE 1PROSITE-ProRule annotationAdd BLAST90
Domaini98 – 174WWE 2PROSITE-ProRule annotationAdd BLAST77

Domaini

The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases.By similarity

Sequence similaritiesi

Belongs to the Deltex family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri412 – 473RING-typePROSITE-ProRule annotationAdd BLAST62

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ86UW9.
KOiK06058.
OMAiQPQGKME.
OrthoDBiEOG091G04KU.
PhylomeDBiQ86UW9.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
PROSITEiView protein in PROSITE
PS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UW9-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMAPSPSLV QVYTSPAAVA VWEWQDGLGT WHPYSATVCS FIEQQFVQQK
60 70 80 90 100
GQRFGLGSLA HSIPLGQADP SLAPYIIDLP SWTQFRQDTG TMRAVRRHLF
110 120 130 140 150
PQHSAPGRGV VWEWLSDDGS WTAYEASVCD YLEQQVARGN QLVDLAPLGY
160 170 180 190 200
NYTVNYTTHT QTNKTSSFCR SVRRQAGPPY PVTTIIAPPG HTGVACSCHQ
210 220 230 240 250
CLSGSRTGPV SGRYRHSMTN LPAYPVPQHP PHRTASVFGT HQAFAPYNKP
260 270 280 290 300
SLSGARSAPR LNTTNAWGAA PPSLGSQPLY RSSLSHLGPQ HLPPGSSTSG
310 320 330 340 350
AVSASLPSGP SSSPGSVPAT VPMQMPKPSR VQQALAGMTS VLMSAIGLPV
360 370 380 390 400
CLSRAPQPTS PPASRLASKS HGSVKRLRKM SVKGATPKPE PEPEQVIKNY
410 420 430 440 450
TEELKVPPDE DCIICMEKLS TASGYSDVTD SKAIGSLAVG HLTKCSHAFH
460 470 480 490 500
LLCLLAMYCN GNKDGSLQCP SCKTIYGEKT GTQPQGKMEV LRFQMSLPGH
510 520 530 540 550
EDCGTILIVY SIPHGIQGPE HPNPGKPFTA RGFPRQCYLP DNAQGRKVLE
560 570 580 590 600
LLKVAWKRRL IFTVGTSSTT GETDTVVWNE IHHKTEMDRN ITGHGYPDPN
610 620
YLQNVLAELA AQGVTEDCLE QQ
Length:622
Mass (Da):67,246
Last modified:June 21, 2005 - v3
Checksum:iABE1398204F2273A
GO
Isoform 2 (identifier: Q86UW9-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     337-383: Missing.

Show »
Length:575
Mass (Da):62,344
Checksum:i449202FB53C026B3
GO

Sequence cautioni

The sequence BAA96052 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01692094A → T. Corresponds to variant dbSNP:rs2462312Ensembl.1
Natural variantiVAR_016921384G → E3 PublicationsCorresponds to variant dbSNP:rs1638152Ensembl.1
Natural variantiVAR_016922421T → A2 PublicationsCorresponds to variant dbSNP:rs6979487Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008350337 – 383Missing in isoform 2. CuratedAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY225124 mRNA. Translation: AAP57518.1.
AY225125 mRNA. Translation: AAP57519.1.
AB040961 mRNA. Translation: BAA96052.1. Different initiation.
AK023924 mRNA. Translation: BAB14727.1.
AC005522 Genomic DNA. Translation: AAP21881.1.
AC007078 Genomic DNA. No translation available.
BC008856 mRNA. Translation: AAH08856.1.
BC018555 mRNA. Translation: AAH18555.1.
BC026059 mRNA. Translation: AAH26059.1.
BC093079 mRNA. Translation: AAH93079.1.
CCDSiCCDS43605.1. [Q86UW9-2]
CCDS5587.1. [Q86UW9-1]
RefSeqiNP_001096064.1. NM_001102594.1. [Q86UW9-1]
NP_001096065.1. NM_001102595.1. [Q86UW9-1]
NP_001096066.1. NM_001102596.1. [Q86UW9-2]
NP_065943.2. NM_020892.2. [Q86UW9-1]
XP_005250188.1. XM_005250131.1. [Q86UW9-1]
XP_005250189.1. XM_005250132.1. [Q86UW9-1]
XP_011514074.1. XM_011515772.1. [Q86UW9-1]
XP_011514075.1. XM_011515773.2. [Q86UW9-1]
XP_011514076.1. XM_011515774.1. [Q86UW9-1]
XP_011514077.1. XM_011515775.1. [Q86UW9-1]
XP_016867212.1. XM_017011723.1. [Q86UW9-1]
XP_016867213.1. XM_017011724.1. [Q86UW9-1]
XP_016867214.1. XM_017011725.1. [Q86UW9-1]
XP_016867215.1. XM_017011726.1. [Q86UW9-2]
XP_016867216.1. XM_017011727.1. [Q86UW9-2]
UniGeneiHs.187058.

Genome annotation databases

EnsembliENST00000324432; ENSP00000322885; ENSG00000091073. [Q86UW9-1]
ENST00000413936; ENSP00000390218; ENSG00000091073. [Q86UW9-1]
ENST00000430490; ENSP00000411986; ENSG00000091073. [Q86UW9-1]
ENST00000446820; ENSP00000392545; ENSG00000091073. [Q86UW9-2]
GeneIDi113878.
KEGGihsa:113878.
UCSCiuc003uff.5. human. [Q86UW9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY225124 mRNA. Translation: AAP57518.1.
AY225125 mRNA. Translation: AAP57519.1.
AB040961 mRNA. Translation: BAA96052.1. Different initiation.
AK023924 mRNA. Translation: BAB14727.1.
AC005522 Genomic DNA. Translation: AAP21881.1.
AC007078 Genomic DNA. No translation available.
BC008856 mRNA. Translation: AAH08856.1.
BC018555 mRNA. Translation: AAH18555.1.
BC026059 mRNA. Translation: AAH26059.1.
BC093079 mRNA. Translation: AAH93079.1.
CCDSiCCDS43605.1. [Q86UW9-2]
CCDS5587.1. [Q86UW9-1]
RefSeqiNP_001096064.1. NM_001102594.1. [Q86UW9-1]
NP_001096065.1. NM_001102595.1. [Q86UW9-1]
NP_001096066.1. NM_001102596.1. [Q86UW9-2]
NP_065943.2. NM_020892.2. [Q86UW9-1]
XP_005250188.1. XM_005250131.1. [Q86UW9-1]
XP_005250189.1. XM_005250132.1. [Q86UW9-1]
XP_011514074.1. XM_011515772.1. [Q86UW9-1]
XP_011514075.1. XM_011515773.2. [Q86UW9-1]
XP_011514076.1. XM_011515774.1. [Q86UW9-1]
XP_011514077.1. XM_011515775.1. [Q86UW9-1]
XP_016867212.1. XM_017011723.1. [Q86UW9-1]
XP_016867213.1. XM_017011724.1. [Q86UW9-1]
XP_016867214.1. XM_017011725.1. [Q86UW9-1]
XP_016867215.1. XM_017011726.1. [Q86UW9-2]
XP_016867216.1. XM_017011727.1. [Q86UW9-2]
UniGeneiHs.187058.

3D structure databases

ProteinModelPortaliQ86UW9.
SMRiQ86UW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125266. 42 interactors.
IntActiQ86UW9. 94 interactors.
MINTiMINT-1436106.
STRINGi9606.ENSP00000322885.

PTM databases

iPTMnetiQ86UW9.
PhosphoSitePlusiQ86UW9.

Polymorphism and mutation databases

BioMutaiDTX2.
DMDMi68067880.

Proteomic databases

EPDiQ86UW9.
MaxQBiQ86UW9.
PaxDbiQ86UW9.
PeptideAtlasiQ86UW9.
PRIDEiQ86UW9.

Protocols and materials databases

DNASUi113878.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000324432; ENSP00000322885; ENSG00000091073. [Q86UW9-1]
ENST00000413936; ENSP00000390218; ENSG00000091073. [Q86UW9-1]
ENST00000430490; ENSP00000411986; ENSG00000091073. [Q86UW9-1]
ENST00000446820; ENSP00000392545; ENSG00000091073. [Q86UW9-2]
GeneIDi113878.
KEGGihsa:113878.
UCSCiuc003uff.5. human. [Q86UW9-1]

Organism-specific databases

CTDi113878.
DisGeNETi113878.
GeneCardsiDTX2.
HGNCiHGNC:15973. DTX2.
HPAiHPA042931.
MIMi613141. gene.
neXtProtiNX_Q86UW9.
OpenTargetsiENSG00000091073.
PharmGKBiPA27515.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEMS. Eukaryota.
ENOG4111EY2. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000007352.
HOVERGENiHBG007213.
InParanoidiQ86UW9.
KOiK06058.
OMAiQPQGKME.
OrthoDBiEOG091G04KU.
PhylomeDBiQ86UW9.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-HSA-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

ChiTaRSiDTX2. human.
GeneWikiiDTX2.
GenomeRNAii113878.
PROiQ86UW9.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000091073.
CleanExiHS_DTX2.
ExpressionAtlasiQ86UW9. baseline and differential.
GenevisibleiQ86UW9. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR004170. WWE-dom.
IPR018123. WWE-dom_subgr.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
PfamiView protein in Pfam
PF02825. WWE. 2 hits.
PF00097. zf-C3HC4. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00678. WWE. 2 hits.
PROSITEiView protein in PROSITE
PS50918. WWE. 2 hits.
PS50089. ZF_RING_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDTX2_HUMAN
AccessioniPrimary (citable) accession number: Q86UW9
Secondary accession number(s): Q6XM87
, Q6XM88, Q96H69, Q9H890, Q9P200
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 21, 2005
Last modified: March 15, 2017
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.