Q86UW8 (HPLN4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan and proteoglycan link protein 4

Alternative name(s):
Brain link protein 2

Gene names

Name:	HAPLN4
Synonyms:	BRAL2, KIAA1926

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Binds to hyaluronic acid and may be involved in formation of the extracellular matrix By similarity. UniProtKB Q80WM4
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Tissue specificity	Expressed predominantly in brain. Ref.1
Sequence similarities	Belongs to the HAPLN family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 2 Link domains.
Sequence caution	The sequence BAB67819.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Immunoglobulin domain Repeat Signal
Ligand	Hyaluronic acid
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hyaluronic acid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 402	373	Hyaluronan and proteoglycan link protein 4		PRO_0000013192
Regions
	Domain	46 – 161	116	Ig-like C2-type
	Domain	163 – 268	106	Link 1
	Domain	273 – 365	93	Link 2
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 143		By similarity UniProtKB P03994
	Disulfide bond	185 ↔ 266		By similarity UniProtKB P03994
	Disulfide bond	209 ↔ 230		By similarity UniProtKB P03994
	Disulfide bond	293 ↔ 363		By similarity UniProtKB P03994
	Disulfide bond	318 ↔ 339		By similarity UniProtKB P03994

Sequences

	Sequence		Length	Mass (Da)
	Q86UW8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B1B6CE2098244124	FASTA	402	42,801
	10 20 30 40 50 60 MVCARAALGP GALWAAAWGV LLLTAPAGAQ RGRKKVVHVL EGESGSVVVQ TAPGQVVSHR 70 80 90 100 110 120 GGTIVLPCRY HYEAAAHGHD GVRLKWTKVV DPLAFTDVFV ALGPQHRAFG SYRGRAELQG 130 140 150 160 170 180 DGPGDASLVL RNVTLQDYGR YECEVTNELE DDAGMVKLDL EGVVFPYHPR GGRYKLTFAE 190 200 210 220 230 240 AQRACAEQDG ILASAEQLHA AWRDGLDWCN AGWLRDGSVQ YPVNRPREPC GGLGGTGSAG 250 260 270 280 290 300 GGGDANGGLR NYGYRHNAEE RYDAFCFTSN LPGRVFFLKP LRPVPFSGAA RACAARGAAV 310 320 330 340 350 360 AKVGQLFAAW KLQLLDRCTA GWLADGSARY PIVNPRARCG GRRPGVRSLG FPDATRRLFG 370 380 390 400 VYCYRAPGAP DPAPGGWGWG WAGGGGWAGG ARDPAAWTPL HV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A hyaluronan binding link protein gene family whose members are physically linked adjacent to chondroitin sulfate proteoglycan core protein genes: the missing links." Spicer A.P., Joo A., Bowling R.A. Jr. J. Biol. Chem. 278:21083-21091(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]	"Molecular cloning of Bral2, a novel brain-specific link protein, and immunohistochemical colocalization with brevican in perineuronal nets." Bekku Y., Su W.-D., Hirakawa S., Faessler R., Ohtsuka A., Kang J.S., Sanders J., Murakami T., Ninomiya Y., Oohashi T. Mol. Cell. Neurosci. 24:148-159(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins." Nagase T., Kikuno R., Ohara O. DNA Res. 8:179-187(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY262756 mRNA. Translation: AAP22048.1. AB107883 mRNA. Translation: BAC79077.1. AB067513 mRNA. Translation: BAB67819.1. Different initiation. CH471106 Genomic DNA. Translation: EAW84804.1. BC142644 mRNA. Translation: AAI42645.1. BC142698 mRNA. Translation: AAI42699.1.
IPI	IPI00044707.
RefSeq	NP_075378.1. NM_023002.2.
UniGene	Hs.367829.
3D structure databases
ProteinModelPortal	Q86UW8.
ModBase	Search...
PTM databases
PhosphoSite	Q86UW8.
Polymorphism databases
DMDM	47605684.
Proteomic databases
PaxDb	Q86UW8.
PRIDE	Q86UW8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000291481; ENSP00000291481; ENSG00000187664.
GeneID	404037.
KEGG	hsa:404037.
UCSC	uc002nmb.3. human.
Organism-specific databases
CTD	404037.
GeneCards	GC19M019366.
HGNC	HGNC:31357. HAPLN4.
HPA	HPA055856.
neXtProt	NX_Q86UW8.
PharmGKB	PA134886721.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG42970.
HOGENOM	HOG000234353.
HOVERGEN	HBG051922.
InParanoid	Q86UW8.
KO	K06853.
OMA	NGGVRNY.
OrthoDB	EOG4G7BZJ.
PhylomeDB	Q86UW8.
Gene expression databases
CleanEx	HS_HAPLN4.
Genevestigator	Q86UW8.
GermOnline	ENSG00000187664. Homo sapiens.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.10.100.10. 2 hits.
InterPro	IPR016186. C-type_lectin-like. IPR016187. C-type_lectin_fold. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003598. Ig_sub2. IPR013106. Ig_V-set. IPR000538. Link. [Graphical view]
Pfam	PF07686. V-set. 1 hit. PF00193. Xlink. 2 hits. [Graphical view]
PRINTS	PR01265. LINKMODULE.
SMART	SM00408. IGc2. 1 hit. SM00445. LINK. 2 hits. [Graphical view]
SUPFAM	SSF56436. C-type_lectin_fold. 2 hits.
PROSITE	PS50835. IG_LIKE. 1 hit. PS01241. LINK_1. 1 hit. PS50963. LINK_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	404037.
NextBio	107801.

Entry information

Entry name

HPLN4_HUMAN

Accession

Primary (citable) accession number: Q86UW8
Secondary accession number(s): A5PKW5, Q96PW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.