Reviewed, UniProtKB/Swiss-Prot Q86UW6 (N4BP2_HUMAN)

Names and origin

Protein names

Recommended name:
    NEDD4-binding protein 2
      Short name=N4BP2
    EC=3.-.-.-
Alternative name(s):
    BCL-3-binding protein

Gene names

Name:	N4BP2
Synonyms:	B3BP, KIAA1413

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1770 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair or recombination.
Subunit structure	Binds NEDD4 By similarity. Binds BCL3 and CREBBP.
Subcellular location	CytoplasmBy similarity.
Domain	The Smr domain has nicking endonuclease activity, but no significant double strand cleavage or exonuclease activity.
Post-translational modification	Ubiquitinated; this targets the protein for degradation by the proteasome By similarity.
Sequence similarities	Contains 1 CUE domain. Contains 1 Smr domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: MGI ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Ref.1 Inferred from direct assay. Source: MGI endonuclease activity Ref.1 Inferred from direct assay. Source: MGI protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86UW6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86UW6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1596-1612: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1770

1770

NEDD4-binding protein 2

PRO_0000096681

Regions

Domain

46 – 89

44

CUE

Domain

1691 – 1770

80

Smr

Nucleotide binding

447 – 454

8

ATP Potential

Coiled coil

90 – 177

88

Potential

Coiled coil

218 – 259

42

Potential

Compositional bias

328 – 368

41

Pro-rich

Amino acid modifications

Modified residue

898

1

Phosphoserine

Natural variations

Alternative sequence

1596 – 1612

17

Missing in isoform 2.

VSP_009721

Natural variant

283

1

P → A in a breast cancer sample; somatic mutation.

VAR_035474

Experimental info

Sequence conflict

118

1

E → K in AAI26467. Ref.2

Sequence conflict

428

1

S → F in BAA91748. Ref.3

Sequence conflict

611

1

D → N in AAP22172. Ref.1

Sequence conflict

611

1

D → N in AAI26467. Ref.2

Sequence conflict

611

1

D → N in BAA92651. Ref.4

Sequence conflict

660

1

K → Q in AAP22172. Ref.1

Sequence conflict

778

1

K → E in BAA92651. Ref.4

Sequence conflict

1353

1

S → R in AAI26467. Ref.2

Sequence conflict

1353

1

S → R in BAA92651. Ref.4

Sequence conflict

1524

1

L → V in AAP22172. Ref.1

Sequence conflict

1591

1

S → F in AAP22172. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

1770

Helix Strand Turn

Details...

Beta strand

1690 – 1692

3

Helix

1698 – 1718

21

Beta strand

1723 – 1728

6

Helix

1733 – 1735

3

Helix

1742 – 1752

11

Beta strand

1765 – 1768

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: 9A49FAB19C1BD56D	FASTA	1,770	198,801
	10 20 30 40 50 60 MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL FTSISEIFSD 70 80 90 100 110 120 LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ SFVASENQVG AAESKIMEKR 130 140 150 160 170 180 PEEESEDSKM DSFLDMQLTE DLDSLIQNAF EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI 190 200 210 220 230 240 NPDSSNMTPI FSTQNMNLNG ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP 250 260 270 280 290 300 EDSLLSSSLN VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL 310 320 330 340 350 360 DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL PLLLPPPPPP 370 380 390 400 410 420 PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS VISHTSPTKV WRNKDGTSAY 430 440 450 460 470 480 QVQETPVSQV VRKKTSYVGL VLVLLRGLPG SGKSFLARTL QEDNPSGVIL STDDYFYING 490 500 510 520 530 540 QYQFDVKYLG EAHEWNQNRA KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR 550 560 570 580 590 600 EPDTWWKFKP KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS 610 620 630 640 650 660 CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV AYLSNADLNK 670 680 690 700 710 720 RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN EQIEMVAVKG YSKTDTDSSM 730 740 750 760 770 780 ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP 790 800 810 820 830 840 RHELSNFVGD WPVDKTIGQR TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ 850 860 870 880 890 900 RGSPHESVED GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM 910 920 930 940 950 960 PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE MLLSEMTCES 970 980 990 1000 1010 1020 QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE QMPKRDPGKE VGMCTQTEPQ 1030 1040 1050 1060 1070 1080 DFALLWKIEK NKISISDSIK VLTGRLDGFK PKVFNINTKS DVQEAIPYRV MYDKSTFVEE 1090 1100 1110 1120 1130 1140 SELTSADESE NLNILCKLFG SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN 1150 1160 1170 1180 1190 1200 FQKSCDGSQI GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN 1210 1220 1230 1240 1250 1260 SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF PGILKATTPK 1270 1280 1290 1300 1310 1320 DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN EEIYFTDSLE IKRNENFPKD 1330 1340 1350 1360 1370 1380 YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA GVSGEDKTEI LNPTPAMAKS LTIDCLELAL 1390 1400 1410 1420 1430 1440 PPELAFQLNE LFGPVGIDSG SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK 1450 1460 1470 1480 1490 1500 FMQDPSLVGH TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE 1510 1520 1530 1540 1550 1560 IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY SLEHTVQFLN 1570 1580 1590 1600 1610 1620 CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK ETEETPSELS FQDFEYPDYD 1630 1640 1650 1660 1670 1680 DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN VATFYAQQGT LHEQKMKEAN HLAAIEIFEK 1690 1700 1710 1720 1730 1740 VNASLLPQNV LDLHGLHVDE ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA 1750 1760 1770 RIKPAVIKYL ISHSFRFSEI KPGCLKVMLK
	Isoform 2 [UniParc]. Checksum: B391A9B622FFCBFF		1,753	196,828
	10 20 30 40 50 60 MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL FTSISEIFSD 70 80 90 100 110 120 LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ SFVASENQVG AAESKIMEKR 130 140 150 160 170 180 PEEESEDSKM DSFLDMQLTE DLDSLIQNAF EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI 190 200 210 220 230 240 NPDSSNMTPI FSTQNMNLNG ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP 250 260 270 280 290 300 EDSLLSSSLN VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL 310 320 330 340 350 360 DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL PLLLPPPPPP 370 380 390 400 410 420 PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS VISHTSPTKV WRNKDGTSAY 430 440 450 460 470 480 QVQETPVSQV VRKKTSYVGL VLVLLRGLPG SGKSFLARTL QEDNPSGVIL STDDYFYING 490 500 510 520 530 540 QYQFDVKYLG EAHEWNQNRA KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR 550 560 570 580 590 600 EPDTWWKFKP KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS 610 620 630 640 650 660 CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV AYLSNADLNK 670 680 690 700 710 720 RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN EQIEMVAVKG YSKTDTDSSM 730 740 750 760 770 780 ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP 790 800 810 820 830 840 RHELSNFVGD WPVDKTIGQR TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ 850 860 870 880 890 900 RGSPHESVED GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM 910 920 930 940 950 960 PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE MLLSEMTCES 970 980 990 1000 1010 1020 QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE QMPKRDPGKE VGMCTQTEPQ 1030 1040 1050 1060 1070 1080 DFALLWKIEK NKISISDSIK VLTGRLDGFK PKVFNINTKS DVQEAIPYRV MYDKSTFVEE 1090 1100 1110 1120 1130 1140 SELTSADESE NLNILCKLFG SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN 1150 1160 1170 1180 1190 1200 FQKSCDGSQI GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN 1210 1220 1230 1240 1250 1260 SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF PGILKATTPK 1270 1280 1290 1300 1310 1320 DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN EEIYFTDSLE IKRNENFPKD 1330 1340 1350 1360 1370 1380 YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA GVSGEDKTEI LNPTPAMAKS LTIDCLELAL 1390 1400 1410 1420 1430 1440 PPELAFQLNE LFGPVGIDSG SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK 1450 1460 1470 1480 1490 1500 FMQDPSLVGH TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE 1510 1520 1530 1540 1550 1560 IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY SLEHTVQFLN 1570 1580 1590 1600 1610 1620 CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKDFEYP DYDDYRAEAF LHQQKRMECY 1630 1640 1650 1660 1670 1680 SKAKEAYRIG KKNVATFYAQ QGTLHEQKMK EANHLAAIEI FEKVNASLLP QNVLDLHGLH 1690 1700 1710 1720 1730 1740 VDEALEHLMR VLEKKTEEFK QNGGKPYLSV ITGRGNHSQG GVARIKPAVI KYLISHSFRF 1750 SEIKPGCLKV MLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of BCL-3-binding protein: implications for transcription and DNA repair or recombination." Watanabe N., Wachi S., Fujita T. J. Biol. Chem. 278:26102-26110(2003) [PubMed: 12730195] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH BCL3 AND CREBBP. Tissue: T-cell.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-659. Tissue: Teratocarcinoma.
[4]	"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O. DNA Res. 7:65-73(2000) [PubMed: 10718198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 355-1770 (ISOFORM 2). Tissue: Brain.
[5]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-898, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Solution structure of the SMR domain of NEDD4-binding protein 2." RIKEN structural genomics initiative (RSGI) Submitted (JUN-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1688-1770.
[7]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ALA-283.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY267013 mRNA. Translation: AAP22172.1.
BC126466 mRNA. Translation: AAI26467.1.
AK001542 mRNA. Translation: BAA91748.1.
AB037834 mRNA. Translation: BAA92651.1.

RefSeq

NP_060647.2.

UniGene

Hs.396494

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2D9I	NMR	-	A	1688-1770	[»]

ModBase

Search...

PTM databases

PhosphoSite

Q86UW6.

Genome annotation databases

Ensembl

ENSG00000078177. Homo sapiens. [Contig view]

GeneID

55728.

KEGG

hsa:55728.

Organism-specific databases

H-InvDB

HIX0004169.

HGNC

HGNC:29851. N4BP2.

HUGE

Search...

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

Q86UW6.

HOVERGEN

Q86UW6.

Gene expression databases

ArrayExpress

Q86UW6.

CleanEx

HS_N4BP2.

GermOnline

ENSG00000078177. Homo sapiens.

Family and domain databases

InterPro

IPR003892. CUE.
IPR013899. DUF1771.
IPR002625. Smr/MutS2_C.
[Graphical view]

Pfam

PF08590. DUF1771. 1 hit.
PF01713. Smr. 1 hit.
[Graphical view]

PROSITE

PS51140. CUE. 1 hit.
PS50828. SMR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

Q86UW6.

NextBio

60645.

Entry information

Entry name

N4BP2_HUMAN

Accession

Primary (citable) accession number: Q86UW6
Secondary accession number(s): A0AVR3, Q9NVK2, Q9P2D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	April 17, 2007
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)