Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NEDD4-binding protein 2

Gene

N4BP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has 5'-polynucleotide kinase and nicking endonuclease activity. May play a role in DNA repair or recombination.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi447 – 4548ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: MGI
  • ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity Source: MGI
  • endonuclease activity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD4-binding protein 2 (EC:3.-.-.-)
Short name:
N4BP2
Alternative name(s):
BCL-3-binding protein
Gene namesi
Name:N4BP2
Synonyms:B3BP, KIAA1413
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:29851. N4BP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162396580.

Polymorphism and mutation databases

BioMutaiN4BP2.
DMDMi145559498.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17701770NEDD4-binding protein 2PRO_0000096681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei906 – 9061PhosphoserineCombined sources
Modified residuei1210 – 12101PhosphothreonineCombined sources

Post-translational modificationi

Ubiquitinated; this targets the protein for degradation by the proteasome.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ86UW6.
MaxQBiQ86UW6.
PaxDbiQ86UW6.
PeptideAtlasiQ86UW6.
PRIDEiQ86UW6.

PTM databases

iPTMnetiQ86UW6.
PhosphoSiteiQ86UW6.

Expressioni

Gene expression databases

BgeeiENSG00000078177.
CleanExiHS_N4BP2.
ExpressionAtlasiQ86UW6. baseline and differential.
GenevisibleiQ86UW6. HS.

Organism-specific databases

HPAiHPA036770.
HPA042607.

Interactioni

Subunit structurei

Binds NEDD4 (By similarity). Binds BCL3 and CREBBP.By similarity

Protein-protein interaction databases

BioGridi120848. 12 interactions.
IntActiQ86UW6. 6 interactions.
STRINGi9606.ENSP00000261435.

Structurei

Secondary structure

1
1770
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1669 – 168517Combined sources
Helixi1686 – 16883Combined sources
Beta strandi1690 – 16923Combined sources
Helixi1698 – 171922Combined sources
Beta strandi1724 – 17285Combined sources
Beta strandi1734 – 17363Combined sources
Helixi1742 – 175211Combined sources
Beta strandi1757 – 17615Combined sources
Beta strandi1764 – 17685Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9INMR-A1688-1770[»]
2VKCNMR-A1657-1770[»]
3BHBX-ray2.20C129-138[»]
3FAUX-ray1.90A/B/C/D1691-1770[»]
ProteinModelPortaliQ86UW6.
SMRiQ86UW6. Positions 1666-1770.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86UW6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 8944CUEPROSITE-ProRule annotationAdd
BLAST
Domaini1691 – 177080SmrPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili90 – 17788Sequence analysisAdd
BLAST
Coiled coili218 – 25942Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi328 – 36841Pro-richAdd
BLAST

Domaini

The Smr domain has nicking endonuclease activity, but no significant double strand cleavage or exonuclease activity.

Sequence similaritiesi

Contains 1 CUE domain.PROSITE-ProRule annotation
Contains 1 Smr domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2401. Eukaryota.
ENOG41101K4. LUCA.
GeneTreeiENSGT00510000047015.
HOGENOMiHOG000113717.
HOVERGENiHBG101251.
InParanoidiQ86UW6.
KOiK15720.
OMAiKDCATKL.
OrthoDBiEOG091G00G9.
PhylomeDBiQ86UW6.
TreeFamiTF327016.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003892. CUE.
IPR013899. DUF1771.
IPR027417. P-loop_NTPase.
IPR002625. Smr_dom.
IPR009060. UBA-like.
[Graphical view]
PfamiPF08590. DUF1771. 1 hit.
PF01713. Smr. 1 hit.
[Graphical view]
SMARTiSM01162. DUF1771. 1 hit.
SM00463. SMR. 1 hit.
[Graphical view]
SUPFAMiSSF160443. SSF160443. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51140. CUE. 1 hit.
PS50828. SMR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UW6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL
60 70 80 90 100
FTSISEIFSD LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ
110 120 130 140 150
SFVASENQVG AAESKIMEKR PEEESEDSKM DSFLDMQLTE DLDSLIQNAF
160 170 180 190 200
EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI NPDSSNMTPI FSTQNMNLNG
210 220 230 240 250
ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP EDSLLSSSLN
260 270 280 290 300
VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL
310 320 330 340 350
DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL
360 370 380 390 400
PLLLPPPPPP PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS
410 420 430 440 450
VISHTSPTKV WRNKDGTSAY QVQETPVSQV VRKKTSYVGL VLVLLRGLPG
460 470 480 490 500
SGKSFLARTL QEDNPSGVIL STDDYFYING QYQFDVKYLG EAHEWNQNRA
510 520 530 540 550
KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR EPDTWWKFKP
560 570 580 590 600
KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS
610 620 630 640 650
CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV
660 670 680 690 700
AYLSNADLNK RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN
710 720 730 740 750
EQIEMVAVKG YSKTDTDSSM ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS
760 770 780 790 800
SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP RHELSNFVGD WPVDKTIGQR
810 820 830 840 850
TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ RGSPHESVED
860 870 880 890 900
GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM
910 920 930 940 950
PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE
960 970 980 990 1000
MLLSEMTCES QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE
1010 1020 1030 1040 1050
QMPKRDPGKE VGMCTQTEPQ DFALLWKIEK NKISISDSIK VLTGRLDGFK
1060 1070 1080 1090 1100
PKVFNINTKS DVQEAIPYRV MYDKSTFVEE SELTSADESE NLNILCKLFG
1110 1120 1130 1140 1150
SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN FQKSCDGSQI
1160 1170 1180 1190 1200
GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN
1210 1220 1230 1240 1250
SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF
1260 1270 1280 1290 1300
PGILKATTPK DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN
1310 1320 1330 1340 1350
EEIYFTDSLE IKRNENFPKD YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA
1360 1370 1380 1390 1400
GVSGEDKTEI LNPTPAMAKS LTIDCLELAL PPELAFQLNE LFGPVGIDSG
1410 1420 1430 1440 1450
SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK FMQDPSLVGH
1460 1470 1480 1490 1500
TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE
1510 1520 1530 1540 1550
IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY
1560 1570 1580 1590 1600
SLEHTVQFLN CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK
1610 1620 1630 1640 1650
ETEETPSELS FQDFEYPDYD DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN
1660 1670 1680 1690 1700
VATFYAQQGT LHEQKMKEAN HLAAIEIFEK VNASLLPQNV LDLHGLHVDE
1710 1720 1730 1740 1750
ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA RIKPAVIKYL
1760 1770
ISHSFRFSEI KPGCLKVMLK
Length:1,770
Mass (Da):198,801
Last modified:April 17, 2007 - v2
Checksum:i9A49FAB19C1BD56D
GO
Isoform 2 (identifier: Q86UW6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1596-1612: Missing.

Note: No experimental confirmation available.
Show »
Length:1,753
Mass (Da):196,828
Checksum:iB391A9B622FFCBFF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181E → K in AAI26467 (PubMed:15489334).Curated
Sequence conflicti428 – 4281S → F in BAA91748 (PubMed:14702039).Curated
Sequence conflicti660 – 6601K → Q in AAP22172 (PubMed:12730195).Curated
Sequence conflicti778 – 7781K → E in BAA92651 (PubMed:10718198).Curated
Sequence conflicti1353 – 13531S → R in AAI26467 (PubMed:15489334).Curated
Sequence conflicti1353 – 13531S → R in BAA92651 (PubMed:10718198).Curated
Sequence conflicti1524 – 15241L → V in AAP22172 (PubMed:12730195).Curated
Sequence conflicti1591 – 15911S → F in AAP22172 (PubMed:12730195).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti101 – 1011S → I.
Corresponds to variant rs17511668 [ dbSNP | Ensembl ].
VAR_051215
Natural varianti196 – 1961M → V.
Corresponds to variant rs10014170 [ dbSNP | Ensembl ].
VAR_051216
Natural varianti283 – 2831P → A in a breast cancer sample; somatic mutation. 1 Publication
VAR_035474
Natural varianti611 – 6111D → N.3 Publications
Corresponds to variant rs794001 [ dbSNP | Ensembl ].
VAR_051217
Natural varianti1587 – 15871T → A.
Corresponds to variant rs2271395 [ dbSNP | Ensembl ].
VAR_051218

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1596 – 161217Missing in isoform 2. 1 PublicationVSP_009721Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY267013 mRNA. Translation: AAP22172.1.
BC126466 mRNA. Translation: AAI26467.1.
AK001542 mRNA. Translation: BAA91748.1.
AB037834 mRNA. Translation: BAA92651.1.
CCDSiCCDS3457.1. [Q86UW6-1]
RefSeqiNP_001305288.1. NM_001318359.1.
NP_060647.2. NM_018177.5. [Q86UW6-1]
XP_011512020.1. XM_011513718.2. [Q86UW6-1]
UniGeneiHs.391463.
Hs.736026.

Genome annotation databases

EnsembliENST00000261435; ENSP00000261435; ENSG00000078177. [Q86UW6-1]
GeneIDi55728.
KEGGihsa:55728.
UCSCiuc003guy.5. human. [Q86UW6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY267013 mRNA. Translation: AAP22172.1.
BC126466 mRNA. Translation: AAI26467.1.
AK001542 mRNA. Translation: BAA91748.1.
AB037834 mRNA. Translation: BAA92651.1.
CCDSiCCDS3457.1. [Q86UW6-1]
RefSeqiNP_001305288.1. NM_001318359.1.
NP_060647.2. NM_018177.5. [Q86UW6-1]
XP_011512020.1. XM_011513718.2. [Q86UW6-1]
UniGeneiHs.391463.
Hs.736026.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9INMR-A1688-1770[»]
2VKCNMR-A1657-1770[»]
3BHBX-ray2.20C129-138[»]
3FAUX-ray1.90A/B/C/D1691-1770[»]
ProteinModelPortaliQ86UW6.
SMRiQ86UW6. Positions 1666-1770.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120848. 12 interactions.
IntActiQ86UW6. 6 interactions.
STRINGi9606.ENSP00000261435.

PTM databases

iPTMnetiQ86UW6.
PhosphoSiteiQ86UW6.

Polymorphism and mutation databases

BioMutaiN4BP2.
DMDMi145559498.

Proteomic databases

EPDiQ86UW6.
MaxQBiQ86UW6.
PaxDbiQ86UW6.
PeptideAtlasiQ86UW6.
PRIDEiQ86UW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261435; ENSP00000261435; ENSG00000078177. [Q86UW6-1]
GeneIDi55728.
KEGGihsa:55728.
UCSCiuc003guy.5. human. [Q86UW6-1]

Organism-specific databases

CTDi55728.
GeneCardsiN4BP2.
H-InvDBHIX0004169.
HGNCiHGNC:29851. N4BP2.
HPAiHPA036770.
HPA042607.
neXtProtiNX_Q86UW6.
PharmGKBiPA162396580.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2401. Eukaryota.
ENOG41101K4. LUCA.
GeneTreeiENSGT00510000047015.
HOGENOMiHOG000113717.
HOVERGENiHBG101251.
InParanoidiQ86UW6.
KOiK15720.
OMAiKDCATKL.
OrthoDBiEOG091G00G9.
PhylomeDBiQ86UW6.
TreeFamiTF327016.

Miscellaneous databases

EvolutionaryTraceiQ86UW6.
GenomeRNAii55728.
PROiQ86UW6.

Gene expression databases

BgeeiENSG00000078177.
CleanExiHS_N4BP2.
ExpressionAtlasiQ86UW6. baseline and differential.
GenevisibleiQ86UW6. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003892. CUE.
IPR013899. DUF1771.
IPR027417. P-loop_NTPase.
IPR002625. Smr_dom.
IPR009060. UBA-like.
[Graphical view]
PfamiPF08590. DUF1771. 1 hit.
PF01713. Smr. 1 hit.
[Graphical view]
SMARTiSM01162. DUF1771. 1 hit.
SM00463. SMR. 1 hit.
[Graphical view]
SUPFAMiSSF160443. SSF160443. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51140. CUE. 1 hit.
PS50828. SMR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiN4BP2_HUMAN
AccessioniPrimary (citable) accession number: Q86UW6
Secondary accession number(s): A0AVR3, Q9NVK2, Q9P2D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.