Q86UW2 (OSTB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Organic solute transporter subunit beta
Short name=OST-beta

Alternative name(s):
Solute carrier family 51 subunit beta

Gene names

Name:	SLC51B
Synonyms:	OSTB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids. Modulates SLC51A glycosylation, membrane trafficking and stability activities. Ref.4
Subunit structure	Interacts with SLC51A. The Ost-alpha/Ost-beta complex is a heterodimer composed of alpha (SLC51A) and beta (SLC51B) subunit; induces the transport of SLC51A from the reticulum endoplasmic to the plasma membrane By similarity.
Subcellular location	Cell membrane; Single-pass membrane protein. Note: Mainly restricted to the lateral and basal membranes of ileal enterocytes. Ref.1 Ref.4
Tissue specificity	Widely expressed with a high expression in ileum. Expressed in testis, colon, liver, small intestine, kidney, ovary and adrenal gland; and at low levels in heart, lung, brain, pituitary, thyroid gland, uterus, prostate, mammary gland and fat. Ref.1 Ref.4
Induction	Positively regulated via NR1H4/FXR. Ref.5 Ref.6
Domain	The transmembrane domain (TM) is the major site of interaction with SLC51A. The extracellular-membrane interface is absolutely required for transport activity. The intracellular-membrane interface is necessary for establishing the correct membrane orientation that is essential for the heterodimer Ost-alpha/Ost-beta complex formation and transport activity at the cell membrane surface By similarity.
Sequence similarities	Belongs to the OST-beta family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bile acid and bile salt transport Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein exit from endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein glycosylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein targeting to membrane Inferred from sequence or structural similarity. Source: UniProtKB regulation of protein stability Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from direct assay Ref.1. Source: MGI protein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein heterodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Organic solute transporter subunit beta		PRO_0000331554
Regions
	Topological domain	1 – 35	35	Extracellular Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Topological domain	57 – 128	72	Cytoplasmic Potential
Experimental info
	Sequence conflict	66	1	K → T in AAP23992. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q86UW2 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: A6D6F9391CB15794	FASTA	128	14,346
	10 20 30 40 50 60 MEHSEGAPGD PAGTVVPQEL LEEMLWFFRV EDASPWNHSI LALAAVVVII SMVLLGRSIQ 70 80 90 100 110 120 ASRKEKMQPP EKETPEVLHL DEAKDHNSLN NLRETLLSEK PNLAQVELEL KERDVLSVFL PDVPETES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional complementation between a novel mammalian polygenic transport complex and an evolutionarily ancient organic solute transporter, OSTalpha-OSTbeta." Seward D.J., Koh A.S., Boyer J.L., Ballatori N. J. Biol. Chem. 278:27473-27482(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Liver.
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"OSTalpha-OSTbeta: a major basolateral bile acid and steroid transporter in human intestinal, renal, and biliary epithelia." Ballatori N., Christian W.V., Lee J.Y., Dawson P.A., Soroka C.J., Boyer J.L., Madejczyk M.S., Li N. Hepatology 42:1270-1279(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[5]	"The nuclear receptor for bile acids, FXR, transactivates human organic solute transporter-alpha and -beta genes." Landrier J.-F., Eloranta J.J., Vavricka S.R., Kullak-Ublick G.A. Am. J. Physiol. 290:G476-G485(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY NR1H4.
[6]	"FXR regulates organic solute transporters alpha and beta in the adrenal gland, kidney, and intestine." Lee H., Zhang Y., Lee F.Y., Nelson S.F., Gonzalez F.J., Edwards P.A. J. Lipid Res. 47:201-214(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY NR1H4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY194242 mRNA. Translation: AAP23992.1. CH471082 Genomic DNA. Translation: EAW77708.1. BC103842 mRNA. Translation: AAI03843.1. BC103843 mRNA. Translation: AAI03844.1. BC103844 mRNA. Translation: AAI03845.1.
IPI	IPI00328824.
RefSeq	NP_849190.2. NM_178859.3.
UniGene	Hs.27018. Hs.534533.
3D structure databases
ProteinModelPortal	Q86UW2.
ModBase	Search...
Protein family/group databases
TCDB	2.A.82.1.2. organic solute transporter (OST) family.
PTM databases
PhosphoSite	Q86UW2.
Polymorphism databases
DMDM	187471105.
Proteomic databases
PRIDE	Q86UW2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334287; ENSP00000335292; ENSG00000186198.
GeneID	123264.
KEGG	hsa:123264.
UCSC	uc002aog.3. human.
Organism-specific databases
CTD	123264.
GeneCards	GC15P065338.
HGNC	HGNC:29956. SLC51B.
HPA	HPA008533.
MIM	612085. gene.
neXtProt	NX_Q86UW2.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44194.
HOGENOM	HOG000115298.
HOVERGEN	HBG095150.
InParanoid	Q86UW2.
KO	K14361.
OMA	EDATPWN.
OrthoDB	EOG4B5P6K.
PhylomeDB	Q86UW2.
Gene expression databases
Bgee	Q86UW2.
Genevestigator	Q86UW2.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	123264.
NextBio	81097.
SOURCE	Search...

Entry information

Entry name

OSTB_HUMAN

Accession

Primary (citable) accession number: Q86UW2
Secondary accession number(s): Q3SYF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.