ID   TRI74_HUMAN             Reviewed;         250 AA.
AC   Q86UV6; B7WP46;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   27-MAR-2024, entry version 155.
DE   RecName: Full=Tripartite motif-containing protein 74;
DE   AltName: Full=Tripartite motif-containing protein 50C;
GN   Name=TRIM74; Synonyms=TRIM50C;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX   PubMed=18398435; DOI=10.1038/ejhg.2008.68;
RA   Micale L., Fusco C., Augello B., Napolitano L.M.R., Dermitzakis E.T.,
RA   Meroni G., Merla G., Reymond A.;
RT   "Williams-Beuren syndrome TRIM50 encodes an E3 ubiquitin ligase.";
RL   Eur. J. Hum. Genet. 16:1038-1049(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12853948; DOI=10.1038/nature01782;
RA   Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA   Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA   Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA   Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA   Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA   Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA   Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA   Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA   Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA   Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA   Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA   Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA   Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA   Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA   Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA   Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA   Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA   McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA   Wilson R.K.;
RT   "The DNA sequence of human chromosome 7.";
RL   Nature 424:157-164(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT ARG-13.
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- INTERACTION:
CC       Q86UV6-2; Q03989: ARID5A; NbExp=3; IntAct=EBI-10259086, EBI-948603;
CC       Q86UV6-2; P54253: ATXN1; NbExp=6; IntAct=EBI-10259086, EBI-930964;
CC       Q86UV6-2; Q9BXY8: BEX2; NbExp=3; IntAct=EBI-10259086, EBI-745073;
CC       Q86UV6-2; Q96LT7: C9orf72; NbExp=3; IntAct=EBI-10259086, EBI-2961725;
CC       Q86UV6-2; Q96D03: DDIT4L; NbExp=3; IntAct=EBI-10259086, EBI-742054;
CC       Q86UV6-2; Q14192: FHL2; NbExp=3; IntAct=EBI-10259086, EBI-701903;
CC       Q86UV6-2; P63215: GNG3; NbExp=3; IntAct=EBI-10259086, EBI-1046668;
CC       Q86UV6-2; O14964: HGS; NbExp=5; IntAct=EBI-10259086, EBI-740220;
CC       Q86UV6-2; O76011: KRT34; NbExp=3; IntAct=EBI-10259086, EBI-1047093;
CC       Q86UV6-2; P51608: MECP2; NbExp=3; IntAct=EBI-10259086, EBI-1189067;
CC       Q86UV6-2; P19404: NDUFV2; NbExp=3; IntAct=EBI-10259086, EBI-713665;
CC       Q86UV6-2; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-10259086, EBI-741158;
CC       Q86UV6-2; O43482: OIP5; NbExp=3; IntAct=EBI-10259086, EBI-536879;
CC       Q86UV6-2; O14832: PHYH; NbExp=3; IntAct=EBI-10259086, EBI-721853;
CC       Q86UV6-2; Q8ND90: PNMA1; NbExp=3; IntAct=EBI-10259086, EBI-302345;
CC       Q86UV6-2; P78424: POU6F2; NbExp=3; IntAct=EBI-10259086, EBI-12029004;
CC       Q86UV6-2; P0CG20: PRR35; NbExp=3; IntAct=EBI-10259086, EBI-11986293;
CC       Q86UV6-2; Q92530: PSMF1; NbExp=3; IntAct=EBI-10259086, EBI-945916;
CC       Q86UV6-2; Q04864: REL; NbExp=3; IntAct=EBI-10259086, EBI-307352;
CC       Q86UV6-2; Q9BVN2: RUSC1; NbExp=3; IntAct=EBI-10259086, EBI-6257312;
CC       Q86UV6-2; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-10259086, EBI-5235340;
CC       Q86UV6-2; P51687: SUOX; NbExp=3; IntAct=EBI-10259086, EBI-3921347;
CC       Q86UV6-2; Q13148: TARDBP; NbExp=3; IntAct=EBI-10259086, EBI-372899;
CC       Q86UV6-2; Q5VWN6: TASOR2; NbExp=3; IntAct=EBI-10259086, EBI-745958;
CC       Q86UV6-2; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-10259086, EBI-9090990;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q86UV6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q86UV6-2; Sequence=VSP_032087;
CC   -!- SIMILARITY: Belongs to the TRIM/RBCC family. {ECO:0000305}.
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DR   EMBL; AF498999; AAP30736.1; -; mRNA.
DR   EMBL; AC005488; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC033871; AAH33871.1; -; mRNA.
DR   CCDS; CCDS5545.1; -. [Q86UV6-1]
DR   RefSeq; NP_001304744.1; NM_001317815.1. [Q86UV6-1]
DR   RefSeq; NP_942150.1; NM_198853.2. [Q86UV6-1]
DR   RefSeq; XP_011514489.1; XM_011516187.2. [Q86UV6-1]
DR   RefSeq; XP_011514491.1; XM_011516189.2. [Q86UV6-2]
DR   AlphaFoldDB; Q86UV6; -.
DR   SMR; Q86UV6; -.
DR   BioGRID; 132031; 18.
DR   IntAct; Q86UV6; 34.
DR   STRING; 9606.ENSP00000285805; -.
DR   BioMuta; TRIM74; -.
DR   DMDM; 56404879; -.
DR   MassIVE; Q86UV6; -.
DR   PaxDb; 9606-ENSP00000285805; -.
DR   PeptideAtlas; Q86UV6; -.
DR   Antibodypedia; 28389; 115 antibodies from 14 providers.
DR   DNASU; 378108; -.
DR   Ensembl; ENST00000285805.3; ENSP00000285805.3; ENSG00000155428.12. [Q86UV6-1]
DR   Ensembl; ENST00000395244.5; ENSP00000378665.1; ENSG00000155428.12. [Q86UV6-2]
DR   GeneID; 378108; -.
DR   KEGG; hsa:378108; -.
DR   MANE-Select; ENST00000285805.3; ENSP00000285805.3; NM_198853.3; NP_942150.1.
DR   UCSC; uc003tws.1; human. [Q86UV6-1]
DR   AGR; HGNC:17453; -.
DR   CTD; 378108; -.
DR   DisGeNET; 378108; -.
DR   GeneCards; TRIM74; -.
DR   HGNC; HGNC:17453; TRIM74.
DR   HPA; ENSG00000155428; Tissue enhanced (stomach, testis).
DR   MIM; 612550; gene.
DR   neXtProt; NX_Q86UV6; -.
DR   OpenTargets; ENSG00000155428; -.
DR   PharmGKB; PA38454; -.
DR   VEuPathDB; HostDB:ENSG00000155428; -.
DR   eggNOG; KOG2177; Eukaryota.
DR   GeneTree; ENSGT00940000167700; -.
DR   HOGENOM; CLU_013137_6_3_1; -.
DR   InParanoid; Q86UV6; -.
DR   OMA; HEFIRID; -.
DR   OrthoDB; 3453019at2759; -.
DR   PhylomeDB; Q86UV6; -.
DR   TreeFam; TF342569; -.
DR   PathwayCommons; Q86UV6; -.
DR   SignaLink; Q86UV6; -.
DR   SIGNOR; Q86UV6; -.
DR   BioGRID-ORCS; 378108; 44 hits in 1024 CRISPR screens.
DR   ChiTaRS; TRIM74; human.
DR   GenomeRNAi; 378108; -.
DR   Pharos; Q86UV6; Tdark.
DR   PRO; PR:Q86UV6; -.
DR   Proteomes; UP000005640; Chromosome 7.
DR   RNAct; Q86UV6; Protein.
DR   Bgee; ENSG00000155428; Expressed in body of stomach and 93 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IDA:HPA.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR   CDD; cd19787; Bbox2_TRIM50-like; 1.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR027370; Znf-RING_euk.
DR   InterPro; IPR000315; Znf_B-box.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   PANTHER; PTHR24103; E3 UBIQUITIN-PROTEIN LIGASE TRIM; 1.
DR   PANTHER; PTHR24103:SF326; E3 UBIQUITIN-PROTEIN LIGASE TRIM50-RELATED; 1.
DR   Pfam; PF00643; zf-B_box; 1.
DR   Pfam; PF13445; zf-RING_UBOX; 1.
DR   SMART; SM00336; BBOX; 1.
DR   SMART; SM00184; RING; 1.
DR   SUPFAM; SSF57845; B-box zinc-binding domain; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS50119; ZF_BBOX; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
DR   Genevisible; Q86UV6; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Coiled coil; Metal-binding; Reference proteome; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..250
FT                   /note="Tripartite motif-containing protein 74"
FT                   /id="PRO_0000056279"
FT   ZN_FING         16..57
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT   ZN_FING         84..125
FT                   /note="B box-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   COILED          125..169
FT                   /evidence="ECO:0000255"
FT   COILED          204..235
FT                   /evidence="ECO:0000255"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         111
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   BINDING         117
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT   VAR_SEQ         243
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_032087"
FT   VARIANT         13
FT                   /note="W -> R (in dbSNP:rs121966)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_039576"
FT   CONFLICT        128
FT                   /note="V -> I (in Ref. 3; AAH33871)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        159
FT                   /note="K -> N (in Ref. 3; AAH33871)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   250 AA;  28547 MW;  6B2F10940B04AC2B CRC64;
     MAWQVSLLEL EDWLQCPICL EVFKESLMLQ CGHSYCKGCL VSLSYHLDTK VRCPMCWQVV
     DGSSSLPNVS LAWVIEALRL PGDPEPKVCV HHRNPLSLFC EKDQELICGL CGLLGSHQHH
     PVTPVSTVCS RMKEELAALF SELKQEQKKV DELIAKLVKN RTRIVNESDV FSWVIRREFQ
     ELRHPVDEEK ARCLEGIGGH TRGLVASLDM QLEQAQGTRE RLAQAECVLE QFGNEDHHEF
     IWKFHSMASR
//