Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q86UU1 (PHLB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pleckstrin homology-like domain family B member 1
Alternative name(s):
Protein LL5-alpha
Gene names
Name:PHLDB1
Synonyms:KIAA0638, LL5A
ORF Names:DLNB07
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length1377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Domain

The PH domain mediates the binding to phosphoinositides.

Sequence similarities

Contains 1 FHA domain.

Contains 1 PH domain.

Sequence caution

The sequence BAA31613.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAB84896.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86UU1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Minor.
Isoform 2 (identifier: Q86UU1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     913-959: Missing.
     1321-1331: Missing.
Note: Major.
Isoform 3 (identifier: Q86UU1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     913-959: Missing.
     1042-1042: Q → E
     1043-1377: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13771377Pleckstrin homology-like domain family B member 1
PRO_0000053891

Regions

Domain64 – 12562FHA
Domain1256 – 1370115PH
Coiled coil683 – 809127 Potential
Coiled coil1144 – 120865 Potential

Amino acid modifications

Modified residue2201Phosphoserine Ref.13
Modified residue2231Phosphoserine Ref.13
Modified residue3241Phosphoserine Ref.11
Modified residue3811Phosphoserine By similarity
Modified residue4301Phosphoserine Ref.13
Modified residue4431Phosphoserine Ref.13
Modified residue5011Phosphoserine Ref.12 Ref.13 Ref.14
Modified residue5181Phosphoserine By similarity
Modified residue5201Phosphoserine Ref.13 Ref.15
Modified residue5221Phosphothreonine Ref.15
Modified residue5511Phosphoserine Ref.13
Modified residue5551Phosphoserine Ref.13
Modified residue5831Phosphoserine Ref.13

Natural variations

Alternative sequence913 – 95947Missing in isoform 2 and isoform 3.
VSP_016737
Alternative sequence10421Q → E in isoform 3.
VSP_016738
Alternative sequence1043 – 1377335Missing in isoform 3.
VSP_016739
Alternative sequence1321 – 133111Missing in isoform 2.
VSP_016740

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DA2829CC9C2ECD3D

FASTA1,377151,162
        10         20         30         40         50         60 
MDALNRNQIG PGCQTQTMVQ KGPLDLIETG KGLKVQTDKP HLVSLGSGRL STAITLLPLE 

        70         80         90        100        110        120 
EGRTVIGSAA RDISLQGPGL APEHCYIENL RGTLTLYPCG NACTIDGLPV RQPTRLTQGC 

       130        140        150        160        170        180 
MLCLGQSTFL RFNHPAEAKW MKSMIPAGGR APGPPYSPVP AESESLVNGN HTPQTATRGP 

       190        200        210        220        230        240 
SACASHSSLV SSIEKDLQEI MDSLVLEEPG AAGKKPAATS PLSPMANGGR YLLSPPTSPG 

       250        260        270        280        290        300 
AMSVGSSYEN TSPAFSPLSS PASSGSCASH SPSGQEPGPS VPPLVPARSS SYHLALQPPQ 

       310        320        330        340        350        360 
SRPSGARSES PRLSRKGGHE RPPSPGLRGL LTDSPAATVL AEARRATESP RLGGQLPVVA 

       370        380        390        400        410        420 
ISLSEYPASG ALSQPTSIPG SPKFQPPVPA PRNKIGTLQD RPPSPFREPP GSERVLTTSP 

       430        440        450        460        470        480 
SRQLVGRTFS DGLATRTLQP PESPRLGRRG LDSMRELPPL SPSLSRRALS PLPTRTTPDP 

       490        500        510        520        530        540 
KLNREVAESP RPRRWAAHGA SPEDFSLTLG ARGRRTRSPS PTLGESLAPH KGSFSGRLSP 

       550        560        570        580        590        600 
AYSLGSLTGA SPCQSPCVQR KLSSGDLRVP VTRERKNSIT EISDNEDDLL EYHRRQRQER 

       610        620        630        640        650        660 
LREQEMERLE RQRLETILNL CAEYSRADGG PEAGELPSIG EATAALALAG RRPSRGLAGA 

       670        680        690        700        710        720 
SGRSSEEPGV ATQRLWESME RSDEENLKEE CSSTESTQQE HEDAPSTKLQ GEVLALEEER 

       730        740        750        760        770        780 
AQVLGHVEQL KVRVKELEQQ LQESAREAEM ERALLQGERE AERALLQKEQ KAVDQLQEKL 

       790        800        810        820        830        840 
VALETGIQKE RDKEAEALET ETKLFEDLEF QQLERESRVE EERELAGQGL LRSKAELLRS 

       850        860        870        880        890        900 
IAKRKERLAI LDSQAGQIRA QAVQESERLA RDKNASLQLL QKEKEKLTVL ERRYHSLTGG 

       910        920        930        940        950        960 
RPFPKTTSTL KEMEKLLLPA VDLEQWYQEL MAGLGTGPAA ASPHSSPPPL PAKASRQLQV 

       970        980        990       1000       1010       1020 
YRSKMDGEAT SPLPRTRSGP LPSSSGSSSS SSQLSVATLG RSPSPKSALL TQNGTGSLPR 

      1030       1040       1050       1060       1070       1080 
NLAATLQDIE TKRQLALQQK GQQVIEEQRR RLAELKQKAA AEAQCQWDAL HGAAPFPAGP 

      1090       1100       1110       1120       1130       1140 
SGFPPLMHHS ILHHLPAGRE RGEEGEHAYD TLSLESSDSM ETSISTGGNS ACSPDNMSSA 

      1150       1160       1170       1180       1190       1200 
SGLDMGKIEE MEKMLKEAHA EKNRLMESRE REMELRRQAL EEERRRREQV ERRLQSESAR 

      1210       1220       1230       1240       1250       1260 
RQQLVEKEVK MREKQFSQAR PLTRYLPIRK EDFDLKTHIE SSGHGVDTCL HVVLSSKVCR 

      1270       1280       1290       1300       1310       1320 
GYLVKMGGKI KSWKKRWFVF DRLKRTLSYY VDKHETKLKG VIYFQAIEEV YYDHLRSAAK 

      1330       1340       1350       1360       1370 
KRFFRFTMVT ESPNPALTFC VKTHDRLYYM VAPSAEAMRI WMDVIVTGAE GYTQFMN 

« Hide

Isoform 2 [UniParc].

Checksum: FC6698DAB7F99188
Show »

FASTA1,319144,739
Isoform 3 [UniParc].

Checksum: D11D43C8C73BB999
Show »

FASTA995107,381

References

« Hide 'large scale' references
[1]"Identification of a 500-kb region of common allelic loss in chromosome 11q23 in non-MYCN amplified type of neuroblastoma."
Kubo T., Arai Y., Ohira M., Gamou T., Maeno G., Sakiyama T., Toyoda A., Hattori M., Sakaki Y., Nakagawara A., Ohki M.
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.
DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Spleen.
[5]NHLBI resequencing and genotyping service (RS&G)
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Placenta.
[9]"Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities."
Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R.
Biochem. J. 351:19-31(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: BINDING TO PHOSPHOINOSITIDES.
[10]"Identification and characterization of human LL5A gene and mouse Ll5a gene in silico."
Katoh M., Katoh M.
Int. J. Oncol. 23:1477-1483(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS 1 AND 2).
[11]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220; SER-223; SER-430; SER-443; SER-501; SER-520; SER-551; SER-555 AND SER-583, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520 AND THR-522, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB094090 mRNA. Translation: BAC76044.1.
AB014538 mRNA. Translation: BAA31613.2. Different initiation.
AK074070 mRNA. Translation: BAB84896.2. Different initiation.
EF445008 Genomic DNA. Translation: ACA06043.1.
EF445008 Genomic DNA. Translation: ACA06041.1.
AP000941 Genomic DNA. No translation available.
AP002954 Genomic DNA. No translation available.
CH471065 Genomic DNA. Translation: EAW67401.1.
BC098586 mRNA. Translation: AAH98586.1.
CCDSCCDS44750.1. [Q86UU1-2]
CCDS8401.1. [Q86UU1-1]
RefSeqNP_001138230.1. NM_001144758.2. [Q86UU1-1]
NP_001138231.1. NM_001144759.2. [Q86UU1-2]
NP_055972.1. NM_015157.3. [Q86UU1-1]
UniGeneHs.504062.

3D structure databases

ProteinModelPortalQ86UU1.
SMRQ86UU1. Positions 28-139, 1261-1365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid116797. 2 interactions.
STRING9606.ENSP00000354498.

PTM databases

PhosphoSiteQ86UU1.

Polymorphism databases

DMDM74723506.

Proteomic databases

MaxQBQ86UU1.
PaxDbQ86UU1.
PRIDEQ86UU1.

Protocols and materials databases

DNASU23187.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000356063; ENSP00000348359; ENSG00000019144. [Q86UU1-2]
ENST00000361417; ENSP00000354498; ENSG00000019144. [Q86UU1-1]
ENST00000528594; ENSP00000435520; ENSG00000019144. [Q86UU1-3]
ENST00000530994; ENSP00000431508; ENSG00000019144. [Q86UU1-3]
GeneID23187.
KEGGhsa:23187.
UCSCuc001ptr.2. human. [Q86UU1-1]
uc001ptt.3. human. [Q86UU1-2]

Organism-specific databases

CTD23187.
GeneCardsGC11P118478.
HGNCHGNC:23697. PHLDB1.
HPAHPA037959.
HPA038448.
MIM612834. gene.
neXtProtNX_Q86UU1.
PharmGKBPA134917952.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG86245.
HOGENOMHOG000286004.
HOVERGENHBG080571.
InParanoidQ86UU1.
OMARLWESME.
OrthoDBEOG779NX9.
PhylomeDBQ86UU1.
TreeFamTF329165.

Gene expression databases

ArrayExpressQ86UU1.
BgeeQ86UU1.
CleanExHS_PHLDB1.
GenevestigatorQ86UU1.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
2.60.200.20. 1 hit.
InterProIPR000253. FHA_dom.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamPF00169. PH. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPHLDB1. human.
GeneWikiPHLDB1.
GenomeRNAi23187.
NextBio44659.
PROQ86UU1.
SOURCESearch...

Entry information

Entry namePHLB1_HUMAN
AccessionPrimary (citable) accession number: Q86UU1
Secondary accession number(s): B0YJ63 expand/collapse secondary AC list , B0YJ64, O75133, Q4KMF8, Q8TEQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM