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Protein

NLR family member X1

Gene

NLRX1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 173ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-168928. RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways.
R-HSA-936440. Negative regulators of RIG-I/MDA5 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
NLR family member X1
Alternative name(s):
Caterpiller protein 11.3
Short name:
CLR11.3
Nucleotide-binding oligomerization domain protein 26
Nucleotide-binding oligomerization domain protein 5
Nucleotide-binding oligomerization domain protein 9
Gene namesi
Name:NLRX1
Synonyms:NOD26, NOD5, NOD9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:29890. NLRX1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi79671.
OpenTargetsiENSG00000160703.
PharmGKBiPA162398052.

Polymorphism and mutation databases

BioMutaiNLRX1.
DMDMi74759406.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 86MitochondrionAdd BLAST86
ChainiPRO_000029619087 – 975NLR family member X1Add BLAST889

Proteomic databases

EPDiQ86UT6.
MaxQBiQ86UT6.
PaxDbiQ86UT6.
PeptideAtlasiQ86UT6.
PRIDEiQ86UT6.

PTM databases

iPTMnetiQ86UT6.
PhosphoSitePlusiQ86UT6.

Expressioni

Tissue specificityi

Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji and Jurkat cell lines (at protein level).3 Publications

Gene expression databases

BgeeiENSG00000160703.
CleanExiHS_NLRX1.
ExpressionAtlasiQ86UT6. baseline and differential.
GenevisibleiQ86UT6. HS.

Organism-specific databases

HPAiHPA038630.
HPA061516.

Interactioni

Subunit structurei

Homohexamer. Interacts with MAVS.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Sarm1Q6PDS32EBI-3893071,EBI-6117196From a different organism.

Protein-protein interaction databases

BioGridi122796. 27 interactors.
DIPiDIP-60637N.
IntActiQ86UT6. 9 interactors.
MINTiMINT-4995266.
STRINGi9606.ENSP00000292199.

Structurei

Secondary structure

1975
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi672 – 686Combined sources15
Helixi688 – 695Combined sources8
Beta strandi700 – 702Combined sources3
Helixi710 – 720Combined sources11
Beta strandi727 – 731Combined sources5
Helixi739 – 744Combined sources6
Helixi746 – 750Combined sources5
Beta strandi752 – 756Combined sources5
Helixi764 – 775Combined sources12
Beta strandi783 – 785Combined sources3
Helixi792 – 804Combined sources13
Beta strandi811 – 813Combined sources3
Helixi820 – 830Combined sources11
Helixi831 – 833Combined sources3
Beta strandi839 – 841Combined sources3
Helixi849 – 861Combined sources13
Beta strandi867 – 869Combined sources3
Helixi877 – 885Combined sources9
Beta strandi895 – 897Combined sources3
Helixi907 – 920Combined sources14
Helixi926 – 945Combined sources20
Helixi950 – 969Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UN9X-ray2.65A/B/C629-975[»]
ProteinModelPortaliQ86UT6.
SMRiQ86UT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 483NACHTPROSITE-ProRule annotationAdd BLAST324
Domaini667 – 694LRRNTAdd BLAST28
Repeati695 – 718LRR 11 PublicationAdd BLAST24
Repeati724 – 747LRR 21 PublicationAdd BLAST24
Repeati749 – 777LRR 31 PublicationAdd BLAST29
Repeati778 – 801LRR 41 PublicationAdd BLAST24
Repeati811 – 834LRR 51 PublicationAdd BLAST24
Repeati835 – 857LRR 61 PublicationAdd BLAST23
Repeati858 – 877LRR 71 PublicationAdd BLAST20
Repeati878 – 899LRR 81 PublicationAdd BLAST22
Domaini906 – 970LRRCTAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 556Required for interaction with MAVS1 PublicationAdd BLAST482
Regioni556 – 974Required for the repression of MAVS-induced interferon signalingAdd BLAST419

Domaini

The LRRCT domain mediates homodimerization and LRRNT mediates trimerization of the dimers.

Sequence similaritiesi

Belongs to the NLRP family.Curated
Contains 8 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated
Contains 1 NACHT domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
GeneTreeiENSGT00860000133673.
HOVERGENiHBG106320.
InParanoidiQ86UT6.
KOiK12653.
OMAiLHLYFNE.
OrthoDBiEOG091G01CG.
PhylomeDBiQ86UT6.
TreeFamiTF331068.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRWGHHLPRA SWGSGFRRAL QRPDDRIPFL IHWSWPLQGE RPFGPPRAFI
60 70 80 90 100
RHHGSSVDSA PPPGRHGRLF PSASATEAIQ RHRRNLAEWF SRLPREERQF
110 120 130 140 150
GPTFALDTVH VDPVIRESTP DELLRPPAEL ALEHQPPQAG LPPLALSQLF
160 170 180 190 200
NPDACGRRVQ TVVLYGTVGT GKSTLVRKMV LDWCYGRLPA FELLIPFSCE
210 220 230 240 250
DLSSLGPAPA SLCQLVAQRY TPLKEVLPLM AAAGSHLLFV LHGLEHLNLD
260 270 280 290 300
FRLAGTGLCS DPEEPQEPAA IIVNLLRKYM LPQASILVTT RPSAIGRIPS
310 320 330 340 350
KYVGRYGEIC GFSDTNLQKL YFQLRLNQPY CGYAVGGSGV SATPAQRDHL
360 370 380 390 400
VQMLSRNLEG HHQIAAACFL PSYCWLVCAT LHFLHAPTPA GQTLTSIYTS
410 420 430 440 450
FLRLNFSGET LDSTDPSNLS LMAYAARTMG KLAYEGVSSR KTYFSEEDVC
460 470 480 490 500
GCLEAGIRTE EEFQLLHIFR RDALRFFLAP CVEPGRAGTF VFTVPAMQEY
510 520 530 540 550
LAALYIVLGL RKTTLQKVGK EVAELVGRVG EDVSLVLGIM AKLLPLRALP
560 570 580 590 600
LLFNLIKVVP RVFGRMVGKS REAVAQAMVL EMFREEDYYN DDVLDQMGAS
610 620 630 640 650
ILGVEGPRRH PDEPPEDEVF ELFPMFMGGL LSAHNRAVLA QLGCPIKNLD
660 670 680 690 700
ALENAQAIKK KLGKLGRQVL PPSELLDHLF FHYEFQNQRF SAEVLSSLRQ
710 720 730 740 750
LNLAGVRMTP VKCTVVAAVL GSGRHALDEV NLASCQLDPA GLRTLLPVFL
760 770 780 790 800
RARKLGLQLN SLGPEACKDL RDLLLHDQCQ ITTLRLSNNP LTAAGVAVLM
810 820 830 840 850
EGLAGNTSVT HLSLLHTGLG DEGLELLAAQ LDRNRQLQEL NVAYNGAGDT
860 870 880 890 900
AALALARAAR EHPSLELLHL YFNELSSEGR QVLRDLGGAA EGGARVVVSL
910 920 930 940 950
TEGTAVSEYW SVILSEVQRN LNSWDRARVQ RHLELLLRDL EDSRGATLNP
960 970
WRKAQLLRVE GEVRALLEQL GSSGS
Length:975
Mass (Da):107,616
Last modified:June 1, 2003 - v1
Checksum:i25B6624FC13217FF
GO
Isoform 2 (identifier: Q86UT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     869-921: HLYFNELSSE...VILSEVQRNL → QGVAIQMCWK...GLVAPRGRCT
     922-975: Missing.

Note: No experimental confirmation available.
Show »
Length:921
Mass (Da):101,876
Checksum:i1F67611ADB9FAA36
GO

Sequence cautioni

The sequence BAB15075 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAG51714 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG51714 differs from that shown. Reason: Erroneous termination at position 951. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti325R → C in BAG53014 (PubMed:14702039).Curated1
Sequence conflicti575A → T in BAG51714 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03461463P → S.2 PublicationsCorresponds to variant rs643423dbSNPEnsembl.1
Natural variantiVAR_034615125R → L.Corresponds to variant rs3809045dbSNPEnsembl.1
Natural variantiVAR_034616793A → E.3 PublicationsCorresponds to variant rs4245191dbSNPEnsembl.1
Natural variantiVAR_034617843A → S.Corresponds to variant rs35500631dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027158869 – 921HLYFN…VQRNL → QGVAIQMCWKLPLLPYAHLW TPRMPSHWCFLLILMPPLPQ WYDGLVAPRGRCT in isoform 2. 1 PublicationAdd BLAST53
Alternative sequenceiVSP_027159922 – 975Missing in isoform 2. 1 PublicationAdd BLAST54

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY245437 mRNA. Translation: AAP31240.1.
BK001111 mRNA. Translation: DAA01244.1.
EF452237 mRNA. Translation: ABO40480.1.
AB094095 mRNA. Translation: BAC76049.1.
AK025131 mRNA. Translation: BAB15075.1. Different initiation.
AK056454 mRNA. Translation: BAG51714.1. Sequence problems.
AK095247 mRNA. Translation: BAG53014.1.
AK291716 mRNA. Translation: BAF84405.1.
BC013199 mRNA. Translation: AAH13199.3.
BC110890 mRNA. Translation: AAI10891.1.
CCDSiCCDS8416.1. [Q86UT6-1]
RefSeqiNP_001269072.1. NM_001282143.1. [Q86UT6-1]
NP_001269073.1. NM_001282144.1. [Q86UT6-1]
NP_001269287.1. NM_001282358.1. [Q86UT6-1]
NP_078894.2. NM_024618.3. [Q86UT6-1]
XP_005271726.1. XM_005271669.1. [Q86UT6-1]
XP_006718967.1. XM_006718904.1. [Q86UT6-2]
XP_011541282.1. XM_011542980.1. [Q86UT6-1]
UniGeneiHs.524082.

Genome annotation databases

EnsembliENST00000292199; ENSP00000292199; ENSG00000160703. [Q86UT6-1]
ENST00000409109; ENSP00000387334; ENSG00000160703. [Q86UT6-1]
ENST00000409265; ENSP00000386858; ENSG00000160703. [Q86UT6-1]
ENST00000409991; ENSP00000386851; ENSG00000160703. [Q86UT6-1]
ENST00000525863; ENSP00000433442; ENSG00000160703. [Q86UT6-2]
GeneIDi79671.
KEGGihsa:79671.
UCSCiuc001pvu.5. human. [Q86UT6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY245437 mRNA. Translation: AAP31240.1.
BK001111 mRNA. Translation: DAA01244.1.
EF452237 mRNA. Translation: ABO40480.1.
AB094095 mRNA. Translation: BAC76049.1.
AK025131 mRNA. Translation: BAB15075.1. Different initiation.
AK056454 mRNA. Translation: BAG51714.1. Sequence problems.
AK095247 mRNA. Translation: BAG53014.1.
AK291716 mRNA. Translation: BAF84405.1.
BC013199 mRNA. Translation: AAH13199.3.
BC110890 mRNA. Translation: AAI10891.1.
CCDSiCCDS8416.1. [Q86UT6-1]
RefSeqiNP_001269072.1. NM_001282143.1. [Q86UT6-1]
NP_001269073.1. NM_001282144.1. [Q86UT6-1]
NP_001269287.1. NM_001282358.1. [Q86UT6-1]
NP_078894.2. NM_024618.3. [Q86UT6-1]
XP_005271726.1. XM_005271669.1. [Q86UT6-1]
XP_006718967.1. XM_006718904.1. [Q86UT6-2]
XP_011541282.1. XM_011542980.1. [Q86UT6-1]
UniGeneiHs.524082.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UN9X-ray2.65A/B/C629-975[»]
ProteinModelPortaliQ86UT6.
SMRiQ86UT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122796. 27 interactors.
DIPiDIP-60637N.
IntActiQ86UT6. 9 interactors.
MINTiMINT-4995266.
STRINGi9606.ENSP00000292199.

PTM databases

iPTMnetiQ86UT6.
PhosphoSitePlusiQ86UT6.

Polymorphism and mutation databases

BioMutaiNLRX1.
DMDMi74759406.

Proteomic databases

EPDiQ86UT6.
MaxQBiQ86UT6.
PaxDbiQ86UT6.
PeptideAtlasiQ86UT6.
PRIDEiQ86UT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292199; ENSP00000292199; ENSG00000160703. [Q86UT6-1]
ENST00000409109; ENSP00000387334; ENSG00000160703. [Q86UT6-1]
ENST00000409265; ENSP00000386858; ENSG00000160703. [Q86UT6-1]
ENST00000409991; ENSP00000386851; ENSG00000160703. [Q86UT6-1]
ENST00000525863; ENSP00000433442; ENSG00000160703. [Q86UT6-2]
GeneIDi79671.
KEGGihsa:79671.
UCSCiuc001pvu.5. human. [Q86UT6-1]

Organism-specific databases

CTDi79671.
DisGeNETi79671.
GeneCardsiNLRX1.
H-InvDBHIX0010285.
HGNCiHGNC:29890. NLRX1.
HPAiHPA038630.
HPA061516.
MIMi611947. gene.
neXtProtiNX_Q86UT6.
OpenTargetsiENSG00000160703.
PharmGKBiPA162398052.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4308. Eukaryota.
ENOG410ZBX3. LUCA.
GeneTreeiENSGT00860000133673.
HOVERGENiHBG106320.
InParanoidiQ86UT6.
KOiK12653.
OMAiLHLYFNE.
OrthoDBiEOG091G01CG.
PhylomeDBiQ86UT6.
TreeFamiTF331068.

Enzyme and pathway databases

ReactomeiR-HSA-168928. RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways.
R-HSA-936440. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

ChiTaRSiNLRX1. human.
GeneWikiiNLRX1.
GenomeRNAii79671.
PROiQ86UT6.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160703.
CleanExiHS_NLRX1.
ExpressionAtlasiQ86UT6. baseline and differential.
GenevisibleiQ86UT6. HS.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR007111. NACHT_NTPase.
[Graphical view]
PfamiPF13516. LRR_6. 1 hit.
[Graphical view]
PROSITEiPS50837. NACHT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNLRX1_HUMAN
AccessioniPrimary (citable) accession number: Q86UT6
Secondary accession number(s): A8K6Q1
, B3KPK2, B3KTA2, Q7RTR3, Q96D51, Q9H724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.