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Q86UN6 (AKA28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
A-kinase anchor protein 14

Short name=AKAP-14
Alternative name(s):
A-kinase anchor protein 28 kDa
Short name=AKAP 28
Protein kinase A-anchoring protein 14
Short name=PRKA14
Gene names
Name:AKAP14
Synonyms:AKAP28
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length197 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Subunit structure

Binds to type II regulatory subunits (RII). Ref.1

Subcellular location

Cytoplasm Probable.

Tissue specificity

Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia. Ref.1

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processspermatogenesis

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86UN6-1)

Also known as: Isoform A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86UN6-2)

Also known as: Isoform B;

The sequence of this isoform differs from the canonical sequence as follows:
     88-146: Missing.
Isoform 3 (identifier: Q86UN6-3)

Also known as: Isoform C;

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: KC → VS
     90-197: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 197197A-kinase anchor protein 14
PRO_0000064521

Regions

Region35 – 5218RII-binding

Natural variations

Alternative sequence88 – 14659Missing in isoform 2.
VSP_009909
Alternative sequence88 – 892KC → VS in isoform 3.
VSP_009910
Alternative sequence90 – 197108Missing in isoform 3.
VSP_009911

Experimental info

Mutagenesis431L → P: Abolishes RII-binding; when associated with P-47. Ref.1
Mutagenesis471V → P: Abolishes RII-binding; when associated with P-43. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Isoform A) [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 3A76B9C3036EAEB9

FASTA19722,815
        10         20         30         40         50         60 
MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN 

        70         80         90        100        110        120 
PLKNIKWMTH GEFTVEKGLK QIDEYFSKCV SKKCWAHGVE FVERKDLIHS FLYIYYVHWS 

       130        140        150        160        170        180 
ISTADLPVAR ISAGTYFTMK VSKTKPPDAP IVVSYVGDHQ ALVHRPGMVR FRENWQKNLT 

       190 
DAKYSFMESF PFLFNRV 

« Hide

Isoform 2 (Isoform B) [UniParc].

Checksum: ABA7B256A770F7E7
Show »

FASTA13815,995
Isoform 3 (Isoform C) [UniParc].

Checksum: 2C5E377D247E3277
Show »

FASTA8910,187

References

« Hide 'large scale' references
[1]"Characterization of an A-kinase anchoring protein in human ciliary axonemes."
Kultgen P.L., Byrd S.K., Ostrowski L.E., Milgram S.L.
Mol. Biol. Cell 13:4156-4166(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBUNIT, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-43 AND VAL-47.
Tissue: Lung epithelium.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF514780 mRNA. Translation: AAP20825.1.
AF514781 mRNA. Translation: AAP20826.1.
AF514782 mRNA. Translation: AAP20827.1.
BC066357 mRNA. Translation: AAH66357.1.
RefSeqNP_001008534.1. NM_001008534.1.
NP_001008535.1. NM_001008535.1.
NP_848928.1. NM_178813.5.
UniGeneHs.592245.

3D structure databases

ProteinModelPortalQ86UN6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid127707. 1 interaction.
IntActQ86UN6. 1 interaction.
STRING9606.ENSP00000360485.

PTM databases

PhosphoSiteQ86UN6.

Polymorphism databases

DMDM46395790.

Proteomic databases

PaxDbQ86UN6.
PRIDEQ86UN6.

Protocols and materials databases

DNASU158798.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371422; ENSP00000360476; ENSG00000186471. [Q86UN6-3]
ENST00000371423; ENSP00000360477; ENSG00000186471. [Q86UN6-2]
ENST00000371431; ENSP00000360485; ENSG00000186471. [Q86UN6-1]
ENST00000394594; ENSP00000378094; ENSG00000186471. [Q86UN6-3]
GeneID158798.
KEGGhsa:158798.
UCSCuc004esd.3. human. [Q86UN6-3]
uc004ese.3. human. [Q86UN6-1]

Organism-specific databases

CTD158798.
GeneCardsGC0XP119029.
HGNCHGNC:24061. AKAP14.
HPAHPA060999.
MIM300462. gene.
neXtProtNX_Q86UN6.
PharmGKBPA134914893.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG85614.
HOGENOMHOG000293385.
HOVERGENHBG050473.
InParanoidQ86UN6.
KOK16530.
OMATASVYFT.
OrthoDBEOG73FQNR.
PhylomeDBQ86UN6.
TreeFamTF329057.

Gene expression databases

ArrayExpressQ86UN6.
BgeeQ86UN6.
CleanExHS_AKAP14.
GenevestigatorQ86UN6.

Family and domain databases

InterProIPR025663. AKAP_28.
[Graphical view]
PfamPF14469. AKAP28. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi158798.
NextBio87803.
PROQ86UN6.
SOURCESearch...

Entry information

Entry nameAKA28_HUMAN
AccessionPrimary (citable) accession number: Q86UN6
Secondary accession number(s): Q86UN4, Q86UN5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: March 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM