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Protein

A-kinase anchor protein 14

Gene

AKAP14

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

GO - Biological processi

  1. spermatogenesis Source: Ensembl
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 14
Short name:
AKAP-14
Alternative name(s):
A-kinase anchor protein 28 kDa
Short name:
AKAP 28
Protein kinase A-anchoring protein 14
Short name:
PRKA14
Gene namesi
Name:AKAP14
Synonyms:AKAP28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:24061. AKAP14.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. sperm fibrous sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi43 – 431L → P: Abolishes RII-binding; when associated with P-47. 1 Publication
Mutagenesisi47 – 471V → P: Abolishes RII-binding; when associated with P-43. 1 Publication

Organism-specific databases

PharmGKBiPA134914893.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197A-kinase anchor protein 14PRO_0000064521Add
BLAST

Proteomic databases

PaxDbiQ86UN6.
PRIDEiQ86UN6.

PTM databases

PhosphoSiteiQ86UN6.

Expressioni

Tissue specificityi

Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.1 Publication

Gene expression databases

BgeeiQ86UN6.
CleanExiHS_AKAP14.
GenevestigatoriQ86UN6.

Organism-specific databases

HPAiHPA060999.

Interactioni

Subunit structurei

Binds to type II regulatory subunits (RII).1 Publication

Protein-protein interaction databases

BioGridi127707. 11 interactions.
IntActiQ86UN6. 1 interaction.
STRINGi9606.ENSP00000360485.

Structurei

3D structure databases

ProteinModelPortaliQ86UN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 5218RII-bindingAdd
BLAST

Phylogenomic databases

eggNOGiNOG85614.
GeneTreeiENSGT00390000003444.
HOGENOMiHOG000293385.
HOVERGENiHBG050473.
InParanoidiQ86UN6.
KOiK16530.
OMAiRWSIPTR.
OrthoDBiEOG73FQNR.
PhylomeDBiQ86UN6.
TreeFamiTF329057.

Family and domain databases

InterProiIPR025663. AKAP_28.
[Graphical view]
PfamiPF14469. AKAP28. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UN6-1) [UniParc]FASTAAdd to basket

Also known as: Isoform A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY
60 70 80 90 100
AVKIVEEERN PLKNIKWMTH GEFTVEKGLK QIDEYFSKCV SKKCWAHGVE
110 120 130 140 150
FVERKDLIHS FLYIYYVHWS ISTADLPVAR ISAGTYFTMK VSKTKPPDAP
160 170 180 190
IVVSYVGDHQ ALVHRPGMVR FRENWQKNLT DAKYSFMESF PFLFNRV
Length:197
Mass (Da):22,815
Last modified:June 1, 2003 - v1
Checksum:i3A76B9C3036EAEB9
GO
Isoform 2 (identifier: Q86UN6-2) [UniParc]FASTAAdd to basket

Also known as: Isoform B

The sequence of this isoform differs from the canonical sequence as follows:
     88-147: Missing.

Show »
Length:137
Mass (Da):15,898
Checksum:i572CCE6742C45A32
GO
Isoform 3 (identifier: Q86UN6-3) [UniParc]FASTAAdd to basket

Also known as: Isoform C

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: KC → VS
     90-197: Missing.

Show »
Length:89
Mass (Da):10,187
Checksum:i2C5E377D247E3277
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q86UN6-2)
Sequence conflicti87 – 871S → SP in AAP20826 (PubMed:12475942).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei88 – 14760Missing in isoform 2. 1 PublicationVSP_009909Add
BLAST
Alternative sequencei88 – 892KC → VS in isoform 3. 1 PublicationVSP_009910
Alternative sequencei90 – 197108Missing in isoform 3. 1 PublicationVSP_009911Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514780 mRNA. Translation: AAP20825.1.
AF514781 mRNA. Translation: AAP20826.1.
AF514782 mRNA. Translation: AAP20827.1.
AC002477 Genomic DNA. No translation available.
BC066357 mRNA. Translation: AAH66357.1.
CCDSiCCDS14591.1. [Q86UN6-1]
CCDS35376.1. [Q86UN6-2]
CCDS35377.1. [Q86UN6-3]
RefSeqiNP_001008534.1. NM_001008534.1. [Q86UN6-2]
NP_001008535.1. NM_001008535.1. [Q86UN6-3]
NP_848928.1. NM_178813.5. [Q86UN6-1]
UniGeneiHs.592245.

Genome annotation databases

EnsembliENST00000334356; ENSP00000334680; ENSG00000186471. [Q86UN6-2]
ENST00000371422; ENSP00000360476; ENSG00000186471. [Q86UN6-3]
ENST00000371425; ENSP00000360479; ENSG00000186471. [Q86UN6-2]
ENST00000371431; ENSP00000360485; ENSG00000186471. [Q86UN6-1]
GeneIDi158798.
KEGGihsa:158798.
UCSCiuc004esd.3. human. [Q86UN6-3]
uc004ese.3. human. [Q86UN6-1]

Polymorphism databases

DMDMi46395790.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF514780 mRNA. Translation: AAP20825.1.
AF514781 mRNA. Translation: AAP20826.1.
AF514782 mRNA. Translation: AAP20827.1.
AC002477 Genomic DNA. No translation available.
BC066357 mRNA. Translation: AAH66357.1.
CCDSiCCDS14591.1. [Q86UN6-1]
CCDS35376.1. [Q86UN6-2]
CCDS35377.1. [Q86UN6-3]
RefSeqiNP_001008534.1. NM_001008534.1. [Q86UN6-2]
NP_001008535.1. NM_001008535.1. [Q86UN6-3]
NP_848928.1. NM_178813.5. [Q86UN6-1]
UniGeneiHs.592245.

3D structure databases

ProteinModelPortaliQ86UN6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127707. 11 interactions.
IntActiQ86UN6. 1 interaction.
STRINGi9606.ENSP00000360485.

PTM databases

PhosphoSiteiQ86UN6.

Polymorphism databases

DMDMi46395790.

Proteomic databases

PaxDbiQ86UN6.
PRIDEiQ86UN6.

Protocols and materials databases

DNASUi158798.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000334356; ENSP00000334680; ENSG00000186471. [Q86UN6-2]
ENST00000371422; ENSP00000360476; ENSG00000186471. [Q86UN6-3]
ENST00000371425; ENSP00000360479; ENSG00000186471. [Q86UN6-2]
ENST00000371431; ENSP00000360485; ENSG00000186471. [Q86UN6-1]
GeneIDi158798.
KEGGihsa:158798.
UCSCiuc004esd.3. human. [Q86UN6-3]
uc004ese.3. human. [Q86UN6-1]

Organism-specific databases

CTDi158798.
GeneCardsiGC0XP119029.
HGNCiHGNC:24061. AKAP14.
HPAiHPA060999.
MIMi300462. gene.
neXtProtiNX_Q86UN6.
PharmGKBiPA134914893.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG85614.
GeneTreeiENSGT00390000003444.
HOGENOMiHOG000293385.
HOVERGENiHBG050473.
InParanoidiQ86UN6.
KOiK16530.
OMAiRWSIPTR.
OrthoDBiEOG73FQNR.
PhylomeDBiQ86UN6.
TreeFamiTF329057.

Miscellaneous databases

GenomeRNAii158798.
NextBioi87803.
PROiQ86UN6.
SOURCEiSearch...

Gene expression databases

BgeeiQ86UN6.
CleanExiHS_AKAP14.
GenevestigatoriQ86UN6.

Family and domain databases

InterProiIPR025663. AKAP_28.
[Graphical view]
PfamiPF14469. AKAP28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of an A-kinase anchoring protein in human ciliary axonemes."
    Kultgen P.L., Byrd S.K., Ostrowski L.E., Milgram S.L.
    Mol. Biol. Cell 13:4156-4166(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBUNIT, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-43 AND VAL-47.
    Tissue: Lung epithelium.
  2. "The DNA sequence of the human X chromosome."
    Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.
    , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
    Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.

Entry informationi

Entry nameiAKA28_HUMAN
AccessioniPrimary (citable) accession number: Q86UN6
Secondary accession number(s): A6NNZ0, Q86UN4, Q86UN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: June 1, 2003
Last modified: March 4, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.