Reviewed, UniProtKB/Swiss-Prot Q86UN6 (AKA28_HUMAN)

Names and origin

Protein names

Recommended name:
    A-kinase anchor protein 14
Alternative name(s):
    Protein kinase A-anchoring protein 14
    A-kinase anchor protein 28 kDa

Gene names

Name:	AKAP14
Synonyms:	AKAP28

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Subunit structure	Binds to type II regulatory subunits (RII). Ref.1
Subcellular location	Cytoplasm Probable.
Tissue specificity	Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia. Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86UN6-1)

Also known as: Isoform A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86UN6-2)

Also known as: Isoform B;

The sequence of this isoform differs from the canonical sequence as follows:
     88-146: Missing.

Isoform 3 (identifier: Q86UN6-3)

Also known as: Isoform C;

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: KC → VS
     90-197: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	A-kinase anchor protein 14		PRO_0000064521
Regions
	Region	35 – 52	18	RII-binding
Natural variations
	Alternative sequence	88 – 146	59	Missing in isoform 2.		VSP_009909
	Alternative sequence	88 – 89	2	KC → VS in isoform 3.		VSP_009910
	Alternative sequence	90 – 197	108	Missing in isoform 3.		VSP_009911
Experimental info
	Mutagenesis	43	1	L → P: Abolishes RII-binding; when associated with P-47. Ref.1
	Mutagenesis	47	1	V → P: Abolishes RII-binding; when associated with P-43. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Isoform A) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3A76B9C3036EAEB9	FASTA	197	22,815
	10 20 30 40 50 60 MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN 70 80 90 100 110 120 PLKNIKWMTH GEFTVEKGLK QIDEYFSKCV SKKCWAHGVE FVERKDLIHS FLYIYYVHWS 130 140 150 160 170 180 ISTADLPVAR ISAGTYFTMK VSKTKPPDAP IVVSYVGDHQ ALVHRPGMVR FRENWQKNLT 190 DAKYSFMESF PFLFNRV
	Isoform 2 (Isoform B). Checksum: ABA7B256A770F7E7	FASTA	138	15,995
	10 20 30 40 50 60 MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN 70 80 90 100 110 120 PLKNIKWMTH GEFTVEKGLK QIDEYFSPDA PIVVSYVGDH QALVHRPGMV RFRENWQKNL 130 TDAKYSFMES FPFLFNRV
	Isoform 3 (Isoform C). Checksum: 2C5E377D247E3277	FASTA	89	10,187
	10 20 30 40 50 60 MSETQNSTSQ KAMDEDNKAA SQTMPNTQDK NYEDELTQVA LALVEDVINY AVKIVEEERN 70 80 PLKNIKWMTH GEFTVEKGLK QIDEYFSVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of an A-kinase anchoring protein in human ciliary axonemes." Kultgen P.L., Byrd S.K., Ostrowski L.E., Milgram S.L. Mol. Biol. Cell 13:4156-4166(2002) [PubMed: 12475942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBUNIT, TISSUE SPECIFICITY, MUTAGENESIS OF LEU-43 AND VAL-47. Tissue: Lung epithelium.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF514780 mRNA. Translation: AAP20825.1. AF514781 mRNA. Translation: AAP20826.1. AF514782 mRNA. Translation: AAP20827.1. BC066357 mRNA. Translation: AAH66357.1.
IPI	IPI00328282. IPI00384227. IPI00410579.
RefSeq	NP_001008534.1. NP_001008535.1. NP_848928.1.
UniGene	Hs.592245
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86UN6. 1 interaction.
Proteomic databases
PRIDE	Q86UN6.
Genome annotation databases
Ensembl	ENSG00000186471. Homo sapiens. [Contig view]
GeneID	158798.
KEGG	hsa:158798.
Organism-specific databases
GeneCards	GC0XP118913.
H-InvDB	HIX0056179.
HGNC	HGNC:24061. AKAP14.
MIM	300462. gene.
PharmGKB	PA134914893.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q86UN6.
HOVERGEN	Q86UN6.
OMA	Q86UN6. VLTWEYQ.
Gene expression databases
ArrayExpress	Q86UN6.
Bgee	Q86UN6.
CleanEx	HS_AKAP14.
GermOnline	ENSG00000186471. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	87803.
SOURCE	Search...

Entry information

Entry name

AKA28_HUMAN

Accession

Primary (citable) accession number: Q86UN6
Secondary accession number(s): Q86UN4, Q86UN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)