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Q86UL3

- GPAT4_HUMAN

UniProt

Q86UL3 - GPAT4_HUMAN

Protein

Glycerol-3-phosphate acyltransferase 4

Gene

AGPAT6

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.1 Publication

    Catalytic activityi

    Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Inhibited by N-ethylmaleimide (NEM).1 Publication

    Pathwayi

    GO - Molecular functioni

    1. glycerol-3-phosphate O-acyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. acyl-CoA metabolic process Source: UniProtKB
    2. CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
    3. cellular lipid metabolic process Source: Reactome
    4. diacylglycerol metabolic process Source: Ensembl
    5. fatty acid metabolic process Source: Ensembl
    6. glandular epithelial cell maturation Source: Ensembl
    7. glycerophospholipid biosynthetic process Source: Reactome
    8. lactation Source: UniProtKB
    9. lipid biosynthetic process Source: UniProtKB
    10. phosphatidic acid biosynthetic process Source: Reactome
    11. phosphatidylcholine biosynthetic process Source: UniProtKB
    12. phospholipid metabolic process Source: Reactome
    13. regulation of multicellular organism growth Source: Ensembl
    14. small molecule metabolic process Source: Reactome
    15. triglyceride biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.
    UniPathwayiUPA00557; UER00612.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate acyltransferase 4 (EC:2.3.1.15)
    Short name:
    GPAT4
    Alternative name(s):
    1-acylglycerol-3-phosphate O-acyltransferase 6
    Short name:
    1-AGP acyltransferase 6
    Short name:
    1-AGPAT 6
    Acyl-CoA:glycerol-3-phosphate acyltransferase 4
    Lysophosphatidic acid acyltransferase zeta
    Short name:
    LPAAT-zeta
    Gene namesi
    Name:AGPAT6
    Synonyms:GPAT4
    ORF Names:UNQ551/PRO1108
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:20880. AGPAT6.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: UniProtKB
    2. endoplasmic reticulum membrane Source: Reactome
    3. integral component of membrane Source: UniProtKB
    4. membrane Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142672637.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737Sequence AnalysisAdd
    BLAST
    Chaini38 – 456419Glycerol-3-phosphate acyltransferase 4PRO_0000024703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi362 – 3621N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ86UL3.
    PaxDbiQ86UL3.
    PRIDEiQ86UL3.

    PTM databases

    PhosphoSiteiQ86UL3.

    Expressioni

    Tissue specificityi

    Ubiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.2 Publications

    Gene expression databases

    ArrayExpressiQ86UL3.
    BgeeiQ86UL3.
    CleanExiHS_AGPAT6.
    GenevestigatoriQ86UL3.

    Organism-specific databases

    HPAiHPA016471.

    Interactioni

    Protein-protein interaction databases

    BioGridi126493. 3 interactions.
    IntActiQ86UL3. 2 interactions.
    MINTiMINT-2878746.
    STRINGi9606.ENSP00000380184.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86UL3.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei156 – 17621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei180 – 20021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi248 – 2536HXXXXD motif

    Domaini

    The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0204.
    HOGENOMiHOG000265725.
    InParanoidiQ86UL3.
    KOiK13506.
    OMAiITYHDRK.
    OrthoDBiEOG70GMFG.
    PhylomeDBiQ86UL3.
    TreeFamiTF315039.

    Family and domain databases

    InterProiIPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view]
    PfamiPF01553. Acyltransferase. 1 hit.
    [Graphical view]
    SMARTiSM00563. PlsC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q86UL3-1 [UniParc]FASTAAdd to Basket

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    MFLLLPFDSL IVNLLGISLT VLFTLLLVFI IVPAIFGVSF GIRKLYMKSL    50
    LKIFAWATLR MERGAKEKNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS 100
    SKALDNTPEF ELSDIFYFCR KGMETIMDDE VTKRFSAEEL ESWNLLSRTN 150
    YNFQYISLRL TVLWGLGVLI RYCFLLPLRI ALAFTGISLL VVGTTVVGYL 200
    PNGRFKEFMS KHVHLMCYRI CVRALTAIIT YHDRENRPRN GGICVANHTS 250
    PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL 300
    VAKRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI 350
    KYDPQFGDAF WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTREADEDAV 400
    QFANRVKSAI ARQGGLVDLL WDGGLKREKV KDTFKEEQQK LYSKMIVGNH 450
    KDRSRS 456
    Length:456
    Mass (Da):52,071
    Last modified:June 1, 2003 - v1
    Checksum:i5B9DEB2912E989E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF406612 mRNA. Translation: AAP21893.1.
    AY358670 mRNA. Translation: AAQ89033.1.
    BC051377 mRNA. Translation: AAH51377.2.
    BC061884 mRNA. Translation: AAH61884.1.
    CCDSiCCDS6117.1.
    RefSeqiNP_848934.1. NM_178819.3.
    UniGeneiHs.355753.

    Genome annotation databases

    EnsembliENST00000396987; ENSP00000380184; ENSG00000158669.
    GeneIDi137964.
    KEGGihsa:137964.
    UCSCiuc003xnz.2. human.

    Polymorphism databases

    DMDMi68052729.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF406612 mRNA. Translation: AAP21893.1 .
    AY358670 mRNA. Translation: AAQ89033.1 .
    BC051377 mRNA. Translation: AAH51377.2 .
    BC061884 mRNA. Translation: AAH61884.1 .
    CCDSi CCDS6117.1.
    RefSeqi NP_848934.1. NM_178819.3.
    UniGenei Hs.355753.

    3D structure databases

    ProteinModelPortali Q86UL3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126493. 3 interactions.
    IntActi Q86UL3. 2 interactions.
    MINTi MINT-2878746.
    STRINGi 9606.ENSP00000380184.

    PTM databases

    PhosphoSitei Q86UL3.

    Polymorphism databases

    DMDMi 68052729.

    Proteomic databases

    MaxQBi Q86UL3.
    PaxDbi Q86UL3.
    PRIDEi Q86UL3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000396987 ; ENSP00000380184 ; ENSG00000158669 .
    GeneIDi 137964.
    KEGGi hsa:137964.
    UCSCi uc003xnz.2. human.

    Organism-specific databases

    CTDi 137964.
    GeneCardsi GC08P041435.
    HGNCi HGNC:20880. AGPAT6.
    HPAi HPA016471.
    MIMi 608143. gene.
    neXtProti NX_Q86UL3.
    PharmGKBi PA142672637.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0204.
    HOGENOMi HOG000265725.
    InParanoidi Q86UL3.
    KOi K13506.
    OMAi ITYHDRK.
    OrthoDBi EOG70GMFG.
    PhylomeDBi Q86UL3.
    TreeFami TF315039.

    Enzyme and pathway databases

    UniPathwayi UPA00557 ; UER00612 .
    Reactomei REACT_1190. Triglyceride Biosynthesis.
    REACT_120906. Synthesis of PA.

    Miscellaneous databases

    ChiTaRSi AGPAT6. human.
    GenomeRNAii 137964.
    NextBioi 83709.
    PROi Q86UL3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q86UL3.
    Bgeei Q86UL3.
    CleanExi HS_AGPAT6.
    Genevestigatori Q86UL3.

    Family and domain databases

    InterProi IPR002123. Plipid/glycerol_acylTrfase.
    [Graphical view ]
    Pfami PF01553. Acyltransferase. 1 hit.
    [Graphical view ]
    SMARTi SM00563. PlsC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and identification of the human LPAAT-zeta gene, a novel member of the lysophosphatidic acid acyltransferase family."
      Li D., Yu L., Wu H., Shan Y., Guo J., Dang Y., Wei Y., Zhao S.
      J. Hum. Genet. 48:438-442(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, TISSUE SPECIFICITY.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiGPAT4_HUMAN
    AccessioniPrimary (citable) accession number: Q86UL3
    Secondary accession number(s): Q86V89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3