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Protein

Glycerol-3-phosphate acyltransferase 4

Gene

GPAT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.1 Publication

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Inhibited by N-ethylmaleimide (NEM).1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), Lysocardiolipin acyltransferase 1 (LCLAT1)
  3. Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.15 2681
ReactomeiR-HSA-1483166 Synthesis of PA
UniPathwayiUPA00557; UER00612

Chemistry databases

SwissLipidsiSLP:000000280

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase 4Imported (EC:2.3.1.15)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 6
Short name:
1-AGP acyltransferase 6
Short name:
1-AGPAT 6
Acyl-CoA:glycerol-3-phosphate acyltransferase 4
Lysophosphatidic acid acyltransferase zeta
Short name:
LPAAT-zeta
Gene namesi
Name:GPAT4Imported
Synonyms:AGPAT6
ORF Names:UNQ551/PRO1108
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000158669.11
HGNCiHGNC:20880 GPAT4
MIMi608143 gene
neXtProtiNX_Q86UL3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000158669
PharmGKBiPA142672637

Polymorphism and mutation databases

BioMutaiAGPAT6
DMDMi68052729

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000002470338 – 456Glycerol-3-phosphate acyltransferase 4Add BLAST419

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi247N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi327N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi328N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi362N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ86UL3
MaxQBiQ86UL3
PaxDbiQ86UL3
PeptideAtlasiQ86UL3
PRIDEiQ86UL3
TopDownProteomicsiQ86UL3

PTM databases

iPTMnetiQ86UL3
PhosphoSitePlusiQ86UL3
SwissPalmiQ86UL3

Expressioni

Tissue specificityi

Ubiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.2 Publications

Gene expression databases

BgeeiENSG00000158669
CleanExiHS_AGPAT6
ExpressionAtlasiQ86UL3 baseline and differential
GenevisibleiQ86UL3 HS

Organism-specific databases

HPAiHPA016471

Interactioni

Protein-protein interaction databases

BioGridi126493, 15 interactors
IntActiQ86UL3, 10 interactors
MINTiQ86UL3
STRINGi9606.ENSP00000380184

Structurei

3D structure databases

ProteinModelPortaliQ86UL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi248 – 253HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00390000000536
HOGENOMiHOG000265725
InParanoidiQ86UL3
KOiK13506
OMAiKMLVGTQ
OrthoDBiEOG091G04E8
PhylomeDBiQ86UL3
TreeFamiTF315039

Family and domain databases

InterProiView protein in InterPro
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86UL3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLLPFDSL IVNLLGISLT VLFTLLLVFI IVPAIFGVSF GIRKLYMKSL
60 70 80 90 100
LKIFAWATLR MERGAKEKNH QLYKPYTNGI IAKDPTSLEE EIKEIRRSGS
110 120 130 140 150
SKALDNTPEF ELSDIFYFCR KGMETIMDDE VTKRFSAEEL ESWNLLSRTN
160 170 180 190 200
YNFQYISLRL TVLWGLGVLI RYCFLLPLRI ALAFTGISLL VVGTTVVGYL
210 220 230 240 250
PNGRFKEFMS KHVHLMCYRI CVRALTAIIT YHDRENRPRN GGICVANHTS
260 270 280 290 300
PIDVIILASD GYYAMVGQVH GGLMGVIQRA MVKACPHVWF ERSEVKDRHL
310 320 330 340 350
VAKRLTEHVQ DKSKLPILIF PEGTCINNTS VMMFKKGSFE IGATVYPVAI
360 370 380 390 400
KYDPQFGDAF WNSSKYGMVT YLLRMMTSWA IVCSVWYLPP MTREADEDAV
410 420 430 440 450
QFANRVKSAI ARQGGLVDLL WDGGLKREKV KDTFKEEQQK LYSKMIVGNH

KDRSRS
Length:456
Mass (Da):52,071
Last modified:June 1, 2003 - v1
Checksum:i5B9DEB2912E989E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF406612 mRNA Translation: AAP21893.1
AY358670 mRNA Translation: AAQ89033.1
BC051377 mRNA Translation: AAH51377.2
BC061884 mRNA Translation: AAH61884.1
CCDSiCCDS6117.1
RefSeqiNP_848934.1, NM_178819.3
XP_011542694.1, XM_011544392.2
XP_011542695.1, XM_011544393.1
XP_011542696.1, XM_011544394.1
XP_016868531.1, XM_017013042.1
UniGeneiHs.355753

Genome annotation databases

EnsembliENST00000396987; ENSP00000380184; ENSG00000158669
GeneIDi137964
KEGGihsa:137964
UCSCiuc003xnz.3 human

Similar proteinsi

Entry informationi

Entry nameiGPAT4_HUMAN
AccessioniPrimary (citable) accession number: Q86UL3
Secondary accession number(s): Q86V89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

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