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Protein

E3 ubiquitin-protein ligase MARCH3

Gene

MARCH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri63 – 12361RING-CH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase MARCH3 (EC:6.3.2.-)
Alternative name(s):
Membrane-associated RING finger protein 3
Membrane-associated RING-CH protein III
Short name:
MARCH-III
RING finger protein 173
Gene namesi
Name:MARCH3
Synonyms:RNF173
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:28728. MARCH3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei145 – 16521HelicalSequence analysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • early endosome membrane Source: UniProtKB-SubCell
  • endosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134901491.

Polymorphism and mutation databases

DMDMi59798460.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253E3 ubiquitin-protein ligase MARCH3PRO_0000055927Add
BLAST

Proteomic databases

PaxDbiQ86UD3.
PRIDEiQ86UD3.

PTM databases

iPTMnetiQ86UD3.
PhosphoSiteiQ86UD3.

Expressioni

Gene expression databases

BgeeiQ86UD3.
CleanExiHS_MARCH3.
GenevisibleiQ86UD3. HS.

Organism-specific databases

HPAiHPA043406.

Interactioni

Subunit structurei

Interacts with MARCH2 and STX6.By similarity

Protein-protein interaction databases

BioGridi125416. 8 interactions.
IntActiQ86UD3. 7 interactions.
STRINGi9606.ENSP00000309141.

Structurei

3D structure databases

ProteinModelPortaliQ86UD3.
SMRiQ86UD3. Positions 70-125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-CH-type zinc finger domain is required for E3 ligase activity.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 RING-CH-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri63 – 12361RING-CH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000293394.
HOVERGENiHBG052411.
InParanoidiQ86UD3.
KOiK10658.
OMAiTVEDCSS.
OrthoDBiEOG7B05F1.
PhylomeDBiQ86UD3.
TreeFamiTF319557.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UD3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTSRCSHLP EVLPDCTSSA APVVKTVEDC GSLVNGQPQY VMQVSAKDGQ
60 70 80 90 100
LLSTVVRTLA TQSPFNDRPM CRICHEGSSQ EDLLSPCECT GTLGTIHRSC
110 120 130 140 150
LEHWLSSSNT SYCELCHFRF AVERKPRPLV EWLRNPGPQH EKRTLFGDMV
160 170 180 190 200
CFLFITPLAT ISGWLCLRGA VDHLHFSSRL EAVGLIALTV ALFTIYLFWT
210 220 230 240 250
LVSFRYHCRL YNEWRRTNQR VILLIPKSVN VPSNQPSLLG LHSVKRNSKE

TVV
Length:253
Mass (Da):28,504
Last modified:June 1, 2003 - v1
Checksum:i6E090675CEBC8A88
GO
Isoform 2 (identifier: Q86UD3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-138: WLRNPGP → VSKWGTS
     139-253: Missing.

Note: No experimental confirmation available.
Show »
Length:138
Mass (Da):15,176
Checksum:iCEB724ACE5833B21
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti68 – 681R → Q.1 Publication
Corresponds to variant rs34821177 [ dbSNP | Ensembl ].
VAR_053639

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei132 – 1387WLRNPGP → VSKWGTS in isoform 2. 1 PublicationVSP_055451
Alternative sequencei139 – 253115Missing in isoform 2. 1 PublicationVSP_055452Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK290129 mRNA. Translation: BAF82818.1.
AC004507 Genomic DNA. No translation available.
AC005221 Genomic DNA. No translation available.
AC093532 Genomic DNA. No translation available.
AC137794 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62412.1.
CH471062 Genomic DNA. Translation: EAW62413.1.
BC047569 mRNA. Translation: AAH47569.1.
BC146948 mRNA. Translation: AAI46949.1.
BC146964 mRNA. Translation: AAI46965.1.
CCDSiCCDS4141.1. [Q86UD3-1]
RefSeqiNP_848545.1. NM_178450.4. [Q86UD3-1]
UniGeneiHs.132441.

Genome annotation databases

EnsembliENST00000308660; ENSP00000309141; ENSG00000173926. [Q86UD3-1]
ENST00000515241; ENSP00000421979; ENSG00000173926. [Q86UD3-2]
GeneIDi115123.
KEGGihsa:115123.
UCSCiuc003kuf.4. human. [Q86UD3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK290129 mRNA. Translation: BAF82818.1.
AC004507 Genomic DNA. No translation available.
AC005221 Genomic DNA. No translation available.
AC093532 Genomic DNA. No translation available.
AC137794 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW62412.1.
CH471062 Genomic DNA. Translation: EAW62413.1.
BC047569 mRNA. Translation: AAH47569.1.
BC146948 mRNA. Translation: AAI46949.1.
BC146964 mRNA. Translation: AAI46965.1.
CCDSiCCDS4141.1. [Q86UD3-1]
RefSeqiNP_848545.1. NM_178450.4. [Q86UD3-1]
UniGeneiHs.132441.

3D structure databases

ProteinModelPortaliQ86UD3.
SMRiQ86UD3. Positions 70-125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi125416. 8 interactions.
IntActiQ86UD3. 7 interactions.
STRINGi9606.ENSP00000309141.

PTM databases

iPTMnetiQ86UD3.
PhosphoSiteiQ86UD3.

Polymorphism and mutation databases

DMDMi59798460.

Proteomic databases

PaxDbiQ86UD3.
PRIDEiQ86UD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308660; ENSP00000309141; ENSG00000173926. [Q86UD3-1]
ENST00000515241; ENSP00000421979; ENSG00000173926. [Q86UD3-2]
GeneIDi115123.
KEGGihsa:115123.
UCSCiuc003kuf.4. human. [Q86UD3-1]

Organism-specific databases

CTDi115123.
GeneCardsiMARCH3.
HGNCiHGNC:28728. MARCH3.
HPAiHPA043406.
MIMi613333. gene.
neXtProtiNX_Q86UD3.
PharmGKBiPA134901491.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1609. Eukaryota.
COG5183. LUCA.
GeneTreeiENSGT00730000110355.
HOGENOMiHOG000293394.
HOVERGENiHBG052411.
InParanoidiQ86UD3.
KOiK10658.
OMAiTVEDCSS.
OrthoDBiEOG7B05F1.
PhylomeDBiQ86UD3.
TreeFamiTF319557.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

GenomeRNAii115123.
PROiQ86UD3.
SOURCEiSearch...

Gene expression databases

BgeeiQ86UD3.
CleanExiHS_MARCH3.
GenevisibleiQ86UD3. HS.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR033275. MARCH-like.
IPR011016. Znf_RING-CH.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23012. PTHR23012. 1 hit.
PfamiPF12906. RINGv. 1 hit.
[Graphical view]
SMARTiSM00744. RINGv. 1 hit.
[Graphical view]
PROSITEiPS51292. ZF_RING_CH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT GLN-68.
    Tissue: Thalamus.
  2. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  5. "MARCH-III is a novel component of endosomes with properties similar to those of MARCH-II."
    Fukuda H., Nakamura N., Hirose S.
    J. Biochem. 139:137-145(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMARH3_HUMAN
AccessioniPrimary (citable) accession number: Q86UD3
Secondary accession number(s): A8K264, B9EJE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.