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Protein

Radial spoke head protein 3 homolog

Gene

RSPH3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a protein kinase A-anchoring protein that scaffolds the cAMP-dependent protein kinase holoenzyme. May serve as a point of convergence for MAPK and PKA signaling in cilia.1 Publication

Names & Taxonomyi

Protein namesi
Recommended name:
Radial spoke head protein 3 homolog
Alternative name(s):
A-kinase anchor protein RSPH3
Radial spoke head-like protein 2
Gene namesi
Name:RSPH3
Synonyms:RSHL2, RSP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21054. RSPH3.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162402248.

Polymorphism and mutation databases

BioMutaiRSPH3.
DMDMi74750415.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Radial spoke head protein 3 homologPRO_0000313741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei286 – 2861Phosphothreonine; by MAPK11 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ86UC2.
PRIDEiQ86UC2.

PTM databases

PhosphoSiteiQ86UC2.

Expressioni

Gene expression databases

BgeeiQ86UC2.
CleanExiHS_RSPH3.
GenevisibleiQ86UC2. HS.

Organism-specific databases

HPAiHPA039109.
HPA040230.

Interactioni

Subunit structurei

Interacts with phosphorylated MAPK1. Interacts with MEK1. Interact with PKA regulatory subunits PRKAR1A and PRKAR1B.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ROPN1LQ96C743EBI-6873025,EBI-9033237

Protein-protein interaction databases

BioGridi123776. 2 interactions.
IntActiQ86UC2. 3 interactions.
MINTiMINT-1209207.
STRINGi9606.ENSP00000252655.

Structurei

3D structure databases

ProteinModelPortaliQ86UC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili215 – 23925Sequence AnalysisAdd
BLAST
Coiled coili331 – 38555Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the flagellar radial spoke RSP3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG86709.
GeneTreeiENSGT00390000004172.
HOGENOMiHOG000259276.
HOVERGENiHBG067489.
InParanoidiQ86UC2.
OMAiWEIVHKH.
OrthoDBiEOG7B8S49.
PhylomeDBiQ86UC2.
TreeFamiTF324184.

Family and domain databases

InterProiIPR009290. Radial_spoke_3.
[Graphical view]
PANTHERiPTHR21648. PTHR21648. 1 hit.
PfamiPF06098. Radial_spoke_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UC2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTVKPAKAAS LARNLAKRRR TYLGGAAGRS QEPEVPCAAV LPGKPGDRNC
60 70 80 90 100
PEFPPPDRTL GCWATDAAPA AGLCGAGSEP SIAPTSCAGN LPSRPPPLLS
110 120 130 140 150
PLLASRNPCP WHYLHLSGSH NTLAPTCFKA KLHRKRGSQP PDMASALTDR
160 170 180 190 200
TSRAPSTYTY TSRPRALPCQ RSRYRDSLTQ PDEEPMHYGN IMYDRRVIRG
210 220 230 240 250
NTYALQTGPL LGRPDSLELQ RQREARKRAL ARKQAQEQLR PQTPEPVEGR
260 270 280 290 300
KHVDVQTELY LEEIADRIIE VDMECQTDAF LDRPPTPLFI PAKTGKDVAT
310 320 330 340 350
QILEGELFDF DLEVKPVLEV LVGKTIEQSL LEVMEEEELA NLRASQREYE
360 370 380 390 400
ELRNSERAEV QRLEEQERRH REEKERRKKQ QWEIMHKHNE TSQKIAARAF
410 420 430 440 450
AQRYLADLLP SVFGSLRDSG YFYDPIERDI EIGFLPWLMN EVEKTMEYSM
460 470 480 490 500
VGRTVLDMLI REVVEKRLCM YEHGEDTHQS PEPEDEPGGP GAMTESLEAS
510 520 530 540 550
EFLEQSMSQT RELLLDGGYL QRTTYDRRSS QERKFMEERE LLGQDEETAM
560
RKSLGEEELS
Length:560
Mass (Da):63,687
Last modified:June 1, 2003 - v1
Checksum:iEEAC4B64D1099019
GO
Isoform 2 (identifier: Q86UC2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-307: Missing.

Note: No experimental confirmation available.
Show »
Length:464
Mass (Da):52,686
Checksum:iEB69C3CDC21F115D
GO

Sequence cautioni

The sequence AAK26432.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB71544.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI19235.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI19236.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 991L → S in BAB71615 (PubMed:14702039).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti201 – 2011N → S.
Corresponds to variant rs16889320 [ dbSNP | Ensembl ].
VAR_037720
Natural varianti213 – 2131R → Q.
Corresponds to variant rs34582178 [ dbSNP | Ensembl ].
VAR_037721
Natural varianti398 – 3981R → Q.
Corresponds to variant rs10455840 [ dbSNP | Ensembl ].
VAR_037722
Natural varianti439 – 4391M → T.
Corresponds to variant rs768994 [ dbSNP | Ensembl ].
VAR_037723
Natural varianti484 – 4841E → K.
Corresponds to variant rs12204826 [ dbSNP | Ensembl ].
VAR_037724
Natural varianti518 – 5181G → D.
Corresponds to variant rs3756987 [ dbSNP | Ensembl ].
VAR_037725

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei212 – 30796Missing in isoform 2. 1 PublicationVSP_030128Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057655 mRNA. Translation: BAB71544.1. Different initiation.
AK057931 mRNA. Translation: BAB71615.1.
AL035530 Genomic DNA. Translation: CAI19235.1. Different initiation.
AL035530 Genomic DNA. Translation: CAI19236.1. Different initiation.
CH471051 Genomic DNA. Translation: EAW47643.1.
BC050604 mRNA. Translation: AAH50604.1.
AF353618 mRNA. Translation: AAK26432.1. Different initiation.
CCDSiCCDS5260.1. [Q86UC2-1]
RefSeqiNP_114130.3. NM_031924.4. [Q86UC2-1]
XP_005267210.1. XM_005267153.3. [Q86UC2-2]
UniGeneiHs.154628.

Genome annotation databases

EnsembliENST00000252655; ENSP00000252655; ENSG00000130363.
GeneIDi83861.
KEGGihsa:83861.
UCSCiuc003qrx.3. human. [Q86UC2-1]
uc010kju.3. human. [Q86UC2-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057655 mRNA. Translation: BAB71544.1. Different initiation.
AK057931 mRNA. Translation: BAB71615.1.
AL035530 Genomic DNA. Translation: CAI19235.1. Different initiation.
AL035530 Genomic DNA. Translation: CAI19236.1. Different initiation.
CH471051 Genomic DNA. Translation: EAW47643.1.
BC050604 mRNA. Translation: AAH50604.1.
AF353618 mRNA. Translation: AAK26432.1. Different initiation.
CCDSiCCDS5260.1. [Q86UC2-1]
RefSeqiNP_114130.3. NM_031924.4. [Q86UC2-1]
XP_005267210.1. XM_005267153.3. [Q86UC2-2]
UniGeneiHs.154628.

3D structure databases

ProteinModelPortaliQ86UC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123776. 2 interactions.
IntActiQ86UC2. 3 interactions.
MINTiMINT-1209207.
STRINGi9606.ENSP00000252655.

PTM databases

PhosphoSiteiQ86UC2.

Polymorphism and mutation databases

BioMutaiRSPH3.
DMDMi74750415.

Proteomic databases

PaxDbiQ86UC2.
PRIDEiQ86UC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252655; ENSP00000252655; ENSG00000130363.
GeneIDi83861.
KEGGihsa:83861.
UCSCiuc003qrx.3. human. [Q86UC2-1]
uc010kju.3. human. [Q86UC2-2]

Organism-specific databases

CTDi83861.
GeneCardsiGC06M159397.
HGNCiHGNC:21054. RSPH3.
HPAiHPA039109.
HPA040230.
MIMi615876. gene.
neXtProtiNX_Q86UC2.
PharmGKBiPA162402248.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG86709.
GeneTreeiENSGT00390000004172.
HOGENOMiHOG000259276.
HOVERGENiHBG067489.
InParanoidiQ86UC2.
OMAiWEIVHKH.
OrthoDBiEOG7B8S49.
PhylomeDBiQ86UC2.
TreeFamiTF324184.

Miscellaneous databases

GeneWikiiRSPH3.
GenomeRNAii83861.
NextBioi72869.
PROiQ86UC2.
SOURCEiSearch...

Gene expression databases

BgeeiQ86UC2.
CleanExiHS_RSPH3.
GenevisibleiQ86UC2. HS.

Family and domain databases

InterProiIPR009290. Radial_spoke_3.
[Graphical view]
PANTHERiPTHR21648. PTHR21648. 1 hit.
PfamiPF06098. Radial_spoke_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 66-560 (ISOFORM 2).
    Tissue: Epithelium and Trachea.
  2. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skin.
  5. "Molecular characterization of the human radial spoke protein 3 as an A-kinase anchoring protein."
    DeKorte M., Carr D.W.
    Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 52-560 (ISOFORM 1).
    Tissue: Testis.
  6. "Radial spoke protein 3 is a mammalian protein kinase A-anchoring protein that binds ERK1/2."
    Jivan A., Earnest S., Juang Y.C., Cobb M.H.
    J. Biol. Chem. 284:29437-29445(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, PHOSPHORYLATION AT THR-286.

Entry informationi

Entry nameiRSPH3_HUMAN
AccessioniPrimary (citable) accession number: Q86UC2
Secondary accession number(s): Q96LQ5, Q96LX2, Q9BX75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.