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Protein

RPA-interacting protein

Gene

RPAIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 212RIP-typeAdd BLAST76

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein complex binding Source: MGI

GO - Biological processi

  • DNA-dependent DNA replication Source: MGI
  • DNA recombination Source: MGI
  • DNA repair Source: MGI
  • protein import into nucleus Source: MGI
  • response to UV Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RPA-interacting protein
Short name:
hRIP
Gene namesi
Name:RPAIN
Synonyms:RIP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:28641. RPAIN.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleolus Source: HPA
  • nucleus Source: HPA
  • PML body Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114K → R: Abolishes sumoylation; when associated with N-103; R-121 and R-142. 1 Publication1
Mutagenesisi121K → R: Induces a strong decrease in sumoylation; when associated with N-103. Abolishes sumoylation; when associated with N-103; R-114 and R-142. 1 Publication1
Mutagenesisi142K → R: Abolishes sumoylation; when associated with N-103; R-114 and R-121. 1 Publication1

Organism-specific databases

DisGeNETi84268.
PharmGKBiPA145007849.

Polymorphism and mutation databases

BioMutaiRPAIN.
DMDMi74727468.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762991 – 219RPA-interacting proteinAdd BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphoserineCombined sources1
Cross-linki103Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); in isoform 21 Publication
Cross-linki121Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); in isoform 21 Publication

Post-translational modificationi

Isoform 2 is sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ86UA6.
PaxDbiQ86UA6.
PeptideAtlasiQ86UA6.
PRIDEiQ86UA6.

PTM databases

iPTMnetiQ86UA6.
PhosphoSitePlusiQ86UA6.

Expressioni

Tissue specificityi

Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis, prostate, thymus and spleen.1 Publication

Gene expression databases

BgeeiENSG00000129197.
CleanExiHS_RPAIN.
ExpressionAtlasiQ86UA6. baseline and differential.
GenevisibleiQ86UA6. HS.

Organism-specific databases

HPAiHPA023924.
HPA031526.
HPA065832.

Interactioni

Subunit structurei

Interacts with the RPA1 subunit of RPA complex.1 Publication

GO - Molecular functioni

  • protein complex binding Source: MGI

Protein-protein interaction databases

BioGridi123995. 9 interactors.
IntActiQ86UA6. 6 interactors.
STRINGi9606.ENSP00000385814.

Structurei

3D structure databases

ProteinModelPortaliQ86UA6.
SMRiQ86UA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 180Mediates nuclear exportAdd BLAST17

Sequence similaritiesi

Contains 1 RIP-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 212RIP-typeAdd BLAST76

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IX54. Eukaryota.
ENOG4111K6G. LUCA.
HOGENOMiHOG000251596.
HOVERGENiHBG082800.
InParanoidiQ86UA6.
PhylomeDBiQ86UA6.
TreeFamiTF326215.

Family and domain databases

InterProiIPR028156. RIP.
IPR028159. RPA_interact_C_dom.
IPR028155. RPA_interact_central.
IPR028158. RPA_interact_N_dom.
[Graphical view]
PANTHERiPTHR31742. PTHR31742. 1 hit.
PfamiPF14768. RPA_interact_C. 1 hit.
PF14767. RPA_interact_M. 1 hit.
PF14766. RPA_interact_N. 1 hit.
[Graphical view]

Sequences (9)i

Sequence statusi: Complete.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86UA6-1) [UniParc]FASTAAdd to basket
Also known as: Alpha, hRIPalpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS
60 70 80 90 100
GPGNSQNSFL VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE
110 120 130 140 150
LIKQEQSIIS EYEKSLQFDE KCLSIMLAEW EANPLICPVC TKYNLRITSG
160 170 180 190 200
VVVCQCGLSI PSHSSELTEQ KLRACLEGSI NEHSAHCPHT PEFSVTGGTE
210
EKSSLLMSCL ACDTWAVIL
Note: Major isoform with isoform 2.
Length:219
Mass (Da):24,784
Last modified:June 1, 2003 - v1
Checksum:i4AF573893850704E
GO
Isoform 2 (identifier: Q86UA6-2) [UniParc]FASTAAdd to basket
Also known as: Beta, hRIPbeta

The sequence of this isoform differs from the canonical sequence as follows:
     164-210: Missing.

Note: Major isoform with isoform 1.
Show »
Length:172
Mass (Da):19,727
Checksum:i0B6AA34C1429D384
GO
Isoform 3 (identifier: Q86UA6-3) [UniParc]FASTAAdd to basket
Also known as: Gamma2

The sequence of this isoform differs from the canonical sequence as follows:
     143-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. May be due to an intron retention.
Show »
Length:142
Mass (Da):16,525
Checksum:iB08C00B3708D8E58
GO
Isoform 4 (identifier: Q86UA6-4) [UniParc]FASTAAdd to basket
Also known as: Gamma1

The sequence of this isoform differs from the canonical sequence as follows:
     142-145: KYNL → NLLS
     146-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:145
Mass (Da):16,824
Checksum:iA7977D77FC00B370
GO
Isoform 5 (identifier: Q86UA6-5) [UniParc]FASTAAdd to basket
Also known as: Delta2

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → VF
     107-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:106
Mass (Da):12,399
Checksum:i184280661713B68C
GO
Isoform 6 (identifier: Q86UA6-6) [UniParc]FASTAAdd to basket
Also known as: Delta3

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → GL
     107-219: Missing.

Show »
Length:106
Mass (Da):12,323
Checksum:i16B830661713B68C
GO
Isoform 7 (identifier: Q86UA6-7) [UniParc]FASTAAdd to basket
Also known as: Delta1, Delta 4

The sequence of this isoform differs from the canonical sequence as follows:
     105-107: EQS → GTT
     108-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:107
Mass (Da):12,412
Checksum:iA2803830661713B6
GO
Isoform 8 (identifier: Q86UA6-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-219: ACDTWAVIL → VSVSWDPLCGKRDLWLVLFPP

Note: No experimental confirmation available.
Show »
Length:231
Mass (Da):26,221
Checksum:i224CC8977EF906E9
GO
Isoform 9 (identifier: Q86UA6-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-219: YNLRITSGVV...LACDTWAVIL → PVILGL

Note: No experimental confirmation available.
Show »
Length:148
Mass (Da):17,118
Checksum:i67DFE6F2628D308C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_023947103K → N Common polymorphism; results in a decrease in sumoylation. 3 PublicationsCorresponds to variant rs12761dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016402105 – 107EQS → GTT in isoform 7. 2 Publications3
Alternative sequenceiVSP_016403105 – 106EQ → VF in isoform 5. 2 Publications2
Alternative sequenceiVSP_016404105 – 106EQ → GL in isoform 6. 3 Publications2
Alternative sequenceiVSP_016405107 – 219Missing in isoform 5 and isoform 6. 3 PublicationsAdd BLAST113
Alternative sequenceiVSP_016406108 – 219Missing in isoform 7. 2 PublicationsAdd BLAST112
Alternative sequenceiVSP_016407142 – 145KYNL → NLLS in isoform 4. 3 Publications4
Alternative sequenceiVSP_016408143 – 219Missing in isoform 3. 2 PublicationsAdd BLAST77
Alternative sequenceiVSP_046795143 – 219YNLRI…WAVIL → PVILGL in isoform 9. CuratedAdd BLAST77
Alternative sequenceiVSP_016409146 – 219Missing in isoform 4. 3 PublicationsAdd BLAST74
Alternative sequenceiVSP_016410164 – 210Missing in isoform 2. 2 PublicationsAdd BLAST47
Alternative sequenceiVSP_045356211 – 219ACDTWAVIL → VSVSWDPLCGKRDLWLVLFP P in isoform 8. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775314 mRNA. Translation: AAX14368.1.
AY775315 mRNA. Translation: AAX14369.1.
AY775316 mRNA. Translation: AAX14370.1.
AY775317 mRNA. Translation: AAX14371.1.
AY775318 mRNA. Translation: AAX14372.1.
AY775319 mRNA. Translation: AAX14373.1.
AY775320 mRNA. Translation: AAX14374.1.
AY775321 mRNA. Translation: AAX14375.1.
AY775323 mRNA. Translation: AAX14377.1.
AY680654 mRNA. Translation: AAT80872.1.
AY680655 mRNA. Translation: AAT80873.1.
AY680656 mRNA. Translation: AAT80874.1.
AY680657 mRNA. Translation: AAT80875.1.
AY680658 mRNA. Translation: AAT80876.1.
AY680659 mRNA. Translation: AAT80877.1.
AY680660 mRNA. Translation: AAT80878.1.
AK295394 mRNA. Translation: BAG58348.1.
AK300409 mRNA. Translation: BAG62139.1.
AC004148 Genomic DNA. No translation available.
BC004451 mRNA. Translation: AAH04451.1.
BC046349 mRNA. Translation: AAH46349.1.
BC051849 mRNA. Translation: AAH51849.1.
CCDSiCCDS32536.1. [Q86UA6-1]
CCDS54075.1. [Q86UA6-8]
CCDS54076.1. [Q86UA6-2]
CCDS54077.1. [Q86UA6-9]
CCDS54079.1. [Q86UA6-6]
RefSeqiNP_001028174.2. NM_001033002.3.
NP_001153715.1. NM_001160243.1.
NP_001153716.1. NM_001160244.1.
NP_001153718.1. NM_001160246.1.
NP_001153738.1. NM_001160266.1.
UniGeneiHs.462086.
Hs.555866.

Genome annotation databases

EnsembliENST00000536255; ENSP00000439939; ENSG00000129197.
ENST00000539417; ENSP00000446453; ENSG00000129197.
GeneIDi84268.
KEGGihsa:84268.
UCSCiuc002gbw.3. human. [Q86UA6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775314 mRNA. Translation: AAX14368.1.
AY775315 mRNA. Translation: AAX14369.1.
AY775316 mRNA. Translation: AAX14370.1.
AY775317 mRNA. Translation: AAX14371.1.
AY775318 mRNA. Translation: AAX14372.1.
AY775319 mRNA. Translation: AAX14373.1.
AY775320 mRNA. Translation: AAX14374.1.
AY775321 mRNA. Translation: AAX14375.1.
AY775323 mRNA. Translation: AAX14377.1.
AY680654 mRNA. Translation: AAT80872.1.
AY680655 mRNA. Translation: AAT80873.1.
AY680656 mRNA. Translation: AAT80874.1.
AY680657 mRNA. Translation: AAT80875.1.
AY680658 mRNA. Translation: AAT80876.1.
AY680659 mRNA. Translation: AAT80877.1.
AY680660 mRNA. Translation: AAT80878.1.
AK295394 mRNA. Translation: BAG58348.1.
AK300409 mRNA. Translation: BAG62139.1.
AC004148 Genomic DNA. No translation available.
BC004451 mRNA. Translation: AAH04451.1.
BC046349 mRNA. Translation: AAH46349.1.
BC051849 mRNA. Translation: AAH51849.1.
CCDSiCCDS32536.1. [Q86UA6-1]
CCDS54075.1. [Q86UA6-8]
CCDS54076.1. [Q86UA6-2]
CCDS54077.1. [Q86UA6-9]
CCDS54079.1. [Q86UA6-6]
RefSeqiNP_001028174.2. NM_001033002.3.
NP_001153715.1. NM_001160243.1.
NP_001153716.1. NM_001160244.1.
NP_001153718.1. NM_001160246.1.
NP_001153738.1. NM_001160266.1.
UniGeneiHs.462086.
Hs.555866.

3D structure databases

ProteinModelPortaliQ86UA6.
SMRiQ86UA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123995. 9 interactors.
IntActiQ86UA6. 6 interactors.
STRINGi9606.ENSP00000385814.

PTM databases

iPTMnetiQ86UA6.
PhosphoSitePlusiQ86UA6.

Polymorphism and mutation databases

BioMutaiRPAIN.
DMDMi74727468.

Proteomic databases

MaxQBiQ86UA6.
PaxDbiQ86UA6.
PeptideAtlasiQ86UA6.
PRIDEiQ86UA6.

Protocols and materials databases

DNASUi84268.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000536255; ENSP00000439939; ENSG00000129197.
ENST00000539417; ENSP00000446453; ENSG00000129197.
GeneIDi84268.
KEGGihsa:84268.
UCSCiuc002gbw.3. human. [Q86UA6-1]

Organism-specific databases

CTDi84268.
DisGeNETi84268.
GeneCardsiRPAIN.
H-InvDBHIX0013468.
HGNCiHGNC:28641. RPAIN.
HPAiHPA023924.
HPA031526.
HPA065832.
neXtProtiNX_Q86UA6.
PharmGKBiPA145007849.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IX54. Eukaryota.
ENOG4111K6G. LUCA.
HOGENOMiHOG000251596.
HOVERGENiHBG082800.
InParanoidiQ86UA6.
PhylomeDBiQ86UA6.
TreeFamiTF326215.

Miscellaneous databases

ChiTaRSiRPAIN. human.
GeneWikiiRPAIN.
GenomeRNAii84268.
PROiQ86UA6.

Gene expression databases

BgeeiENSG00000129197.
CleanExiHS_RPAIN.
ExpressionAtlasiQ86UA6. baseline and differential.
GenevisibleiQ86UA6. HS.

Family and domain databases

InterProiIPR028156. RIP.
IPR028159. RPA_interact_C_dom.
IPR028155. RPA_interact_central.
IPR028158. RPA_interact_N_dom.
[Graphical view]
PANTHERiPTHR31742. PTHR31742. 1 hit.
PfamiPF14768. RPA_interact_C. 1 hit.
PF14767. RPA_interact_M. 1 hit.
PF14766. RPA_interact_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIP_HUMAN
AccessioniPrimary (citable) accession number: Q86UA6
Secondary accession number(s): B4DI36
, B4DTX7, E9PES3, J3KNH8, Q4G2Y0, Q4G2Y5, Q4G2Y8, Q6B4V9, Q6B4W0, Q6B4W1, Q6B4W4, Q86X49, Q9BT00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.