Q86UA6 (RIP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RPA-interacting protein
Short name=hRIP

Gene names

Name:	RPAIN
Synonyms:	RIP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. Isoform 2 is sumoylated and mediates the localization of RPA complex into the PML body of the nucleus, thereby participating in RPA function in DNA metabolism. Ref.2
Subunit structure	Interacts with the RPA1 subunit of RPA complex. Ref.2
Subcellular location	Isoform 1: Cytoplasm. Nucleus Ref.1 Ref.2. Isoform 2: Nucleus › PML body Ref.1 Ref.2.
Tissue specificity	Widely expressed. Expressed in pancreas, kidney, muscle, liver, lung, placenta, brain, heart, leukocytes, colon, intestine, ovary, testis, prostate, thymus and spleen. Ref.1
Post-translational modification	Isoform 2 is sumoylated. Sumoylation is required for localization in the nuclear PML body and transport of RPA complex in PML body. Upon UV irradiation and during S phase, it is desumoylated, releasing RPA complex that is translocated to sites of DNA damage. Sumoylation takes place at different Lys residues. Ref.2
Sequence similarities	Contains 1 RIP-type zinc finger.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from physical interaction Ref.2. Source: MGI DNA repair Inferred from physical interaction Ref.2. Source: MGI DNA-dependent DNA replication Inferred from physical interaction Ref.2. Source: MGI protein import into nucleus Inferred from direct assay Ref.2. Source: MGI response to UV Inferred from direct assay Ref.2. Source: MGI
Cellular_component	PML body Inferred from direct assay Ref.2. Source: MGI cytoplasm Inferred from direct assay Ref.2. Source: MGI nucleolus Inferred from direct assay. Source: HPA
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 8 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86UA6-1)

Also known as: Alpha; hRIPalpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Major isoform with isoform 2.

Isoform 2 (identifier: Q86UA6-2)

Also known as: Beta; hRIPbeta;

The sequence of this isoform differs from the canonical sequence as follows:
     164-210: Missing.

Note: Major isoform with isoform 1.

Isoform 3 (identifier: Q86UA6-3)

Also known as: Gamma2;

The sequence of this isoform differs from the canonical sequence as follows:
     143-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. May be due to an intron retention.

Isoform 4 (identifier: Q86UA6-4)

Also known as: Gamma1;

The sequence of this isoform differs from the canonical sequence as follows:
     142-145: KYNL → NLLS
     146-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 5 (identifier: Q86UA6-5)

Also known as: Delta2;

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → VF
     107-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 6 (identifier: Q86UA6-6)

Also known as: Delta3;

The sequence of this isoform differs from the canonical sequence as follows:
     105-106: EQ → GL
     107-219: Missing.

Isoform 7 (identifier: Q86UA6-7)

Also known as: Delta1; Delta 4;

The sequence of this isoform differs from the canonical sequence as follows:
     105-107: EQS → GTT
     108-219: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Isoform 8 (identifier: Q86UA6-8)

The sequence of this isoform differs from the canonical sequence as follows:
     211-219: ACDTWAVIL → VSVSWDPLCGKRDLWLVLFPP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	RPA-interacting protein		PRO_0000076299
Regions
	Zinc finger	137 – 212	76	RIP-type
	Region	164 – 180	17	Mediates nuclear export
Amino acid modifications
	Cross-link	103		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); in isoform 2 Probable
	Cross-link	121		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); in isoform 2 Probable
Natural variations
	Alternative sequence	105 – 107	3	EQS → GTT in isoform 7.		VSP_016402
	Alternative sequence	105 – 106	2	EQ → VF in isoform 5.		VSP_016403
	Alternative sequence	105 – 106	2	EQ → GL in isoform 6.		VSP_016404
	Alternative sequence	107 – 219	113	Missing in isoform 5 and isoform 6.		VSP_016405
	Alternative sequence	108 – 219	112	Missing in isoform 7.		VSP_016406
	Alternative sequence	142 – 145	4	KYNL → NLLS in isoform 4.		VSP_016407
	Alternative sequence	143 – 219	77	Missing in isoform 3.		VSP_016408
	Alternative sequence	146 – 219	74	Missing in isoform 4.		VSP_016409
	Alternative sequence	164 – 210	47	Missing in isoform 2.		VSP_016410
	Alternative sequence	211 – 219	9	ACDTWAVIL → VSVSWDPLCGKRDLWLVLFP P in isoform 8.		VSP_045356
	Natural variant	103	1	K → N Common polymorphism; results in a decrease in sumoylation. Ref.2 Ref.3 Ref.5 Corresponds to variant rs12761 [ dbSNP | Ensembl ].		VAR_023947
Experimental info
	Mutagenesis	114	1	K → R: Abolishes sumoylation; when associated with N-103; R-121 and R-142. Ref.2
	Mutagenesis	121	1	K → R: Induces a strong decrease in sumoylation; when associated with N-103. Abolishes sumoylation; when associated with N-103; R-114 and R-142. Ref.2
	Mutagenesis	142	1	K → R: Abolishes sumoylation; when associated with N-103; R-114 and R-121. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha) (hRIPalpha) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4AF573893850704E	FASTA	219	24,784
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 110 120 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQEQSIIS EYEKSLQFDE 130 140 150 160 170 180 KCLSIMLAEW EANPLICPVC TKYNLRITSG VVVCQCGLSI PSHSSELTEQ KLRACLEGSI 190 200 210 NEHSAHCPHT PEFSVTGGTE EKSSLLMSCL ACDTWAVIL
	Isoform 2 (Beta) (hRIPbeta) [UniParc]. Checksum: 0B6AA34C1429D384	FASTA	172	19,727
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 110 120 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQEQSIIS EYEKSLQFDE 130 140 150 160 170 KCLSIMLAEW EANPLICPVC TKYNLRITSG VVVCQCGLSI PSHACDTWAV IL
	Isoform 3 (Gamma2) [UniParc]. Checksum: B08C00B3708D8E58	FASTA	142	16,525
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 110 120 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQEQSIIS EYEKSLQFDE 130 140 KCLSIMLAEW EANPLICPVC TK
	Isoform 4 (Gamma1) [UniParc]. Checksum: A7977D77FC00B370	FASTA	145	16,824
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 110 120 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQEQSIIS EYEKSLQFDE 130 140 KCLSIMLAEW EANPLICPVC TNLLS
	Isoform 5 (Delta2) [UniParc]. Checksum: 184280661713B68C	FASTA	106	12,399
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQVF
	Isoform 6 (Delta3) [UniParc]. Checksum: 16B830661713B68C	FASTA	106	12,323
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQGL
	Isoform 7 (Delta1) (Delta 4) [UniParc]. Checksum: A2803830661713B6	FASTA	107	12,412
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQGTT
	Isoform 8 [UniParc]. Checksum: 224CC8977EF906E9	FASTA	231	26,221
	10 20 30 40 50 60 MAESLRSPRR SLYKLVGSPP WKEAFRQRCL ERMRNSRDRL LNRYRQAGSS GPGNSQNSFL 70 80 90 100 110 120 VQEVMEEEWN ALQSVENCPE DLAQLEELID MAVLEEIQQE LIKQEQSIIS EYEKSLQFDE 130 140 150 160 170 180 KCLSIMLAEW EANPLICPVC TKYNLRITSG VVVCQCGLSI PSHSSELTEQ KLRACLEGSI 190 200 210 220 230 NEHSAHCPHT PEFSVTGGTE EKSSLLMSCL VSVSWDPLCG KRDLWLVLFP P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, expression pattern, and subcellular location of human RIP isoforms." Chen J.-Z., Huang S.-D., Ji C.-N., Pang R.-Y., Xie Y., Xue J.-L. DNA Cell Biol. 24:464-469(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5; 6 AND 7), SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"Sumoylation of the novel protein hRIPbeta is involved in replication protein A deposition in PML nuclear bodies." Park J., Seo T., Kim H., Choe J. Mol. Cell. Biol. 25:8202-8214(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 4; 5; 6 AND 7), FUNCTION, SUBCELLULAR LOCATION, PROBABLE SUMOYLATION AT LYS-103 AND LYS-121, INTERACTION WITH RPA1, MUTAGENESIS OF LYS-114; LYS-121 AND LYS-142, VARIANT ASN-103.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 8), VARIANT ASN-103. Tissue: Corpus callosum and Placenta.
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 4 AND 6), VARIANT ASN-103. Tissue: Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY775314 mRNA. Translation: AAX14368.1. AY775315 mRNA. Translation: AAX14369.1. AY775316 mRNA. Translation: AAX14370.1. AY775317 mRNA. Translation: AAX14371.1. AY775318 mRNA. Translation: AAX14372.1. AY775319 mRNA. Translation: AAX14373.1. AY775320 mRNA. Translation: AAX14374.1. AY775321 mRNA. Translation: AAX14375.1. AY775323 mRNA. Translation: AAX14377.1. AY680654 mRNA. Translation: AAT80872.1. AY680655 mRNA. Translation: AAT80873.1. AY680656 mRNA. Translation: AAT80874.1. AY680657 mRNA. Translation: AAT80875.1. AY680658 mRNA. Translation: AAT80876.1. AY680659 mRNA. Translation: AAT80877.1. AY680660 mRNA. Translation: AAT80878.1. AK295394 mRNA. Translation: BAG58348.1. AK300409 mRNA. Translation: BAG62139.1. AC004148 Genomic DNA. No translation available. BC004451 mRNA. Translation: AAH04451.1. BC046349 mRNA. Translation: AAH46349.1. BC051849 mRNA. Translation: AAH51849.1.
IPI	IPI00465161. IPI00651764. IPI00655684. IPI00655734. IPI00655768. IPI00655860. IPI00655914. IPI00930099.
RefSeq	NP_001028174.2. NM_001033002.3. NP_001153715.1. NM_001160243.1. NP_001153716.1. NM_001160244.1. NP_001153718.1. NM_001160246.1. NP_001153738.1. NM_001160266.1.
UniGene	Hs.462086. Hs.555866.
3D structure databases
ProteinModelPortal	Q86UA6.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86UA6. 4 interactions.
PTM databases
PhosphoSite	Q86UA6.
Polymorphism databases
DMDM	74727468.
Proteomic databases
PaxDb	Q86UA6.
PRIDE	Q86UA6.
Protocols and materials databases
DNASU	84268.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000536255; ENSP00000439939; ENSG00000129197. ENST00000539417; ENSP00000446453; ENSG00000129197.
GeneID	84268.
KEGG	hsa:84268.
Organism-specific databases
CTD	84268.
GeneCards	GC17P005263.
H-InvDB	HIX0013468.
HGNC	HGNC:28641. RPAIN.
HPA	HPA023924. HPA031526.
neXtProt	NX_Q86UA6.
PharmGKB	PA145007849.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG46030.
HOVERGEN	HBG082800.
InParanoid	Q86UA6.
OMA	VMEEEWN.
OrthoDB	EOG4FBHV1.
Gene expression databases
ArrayExpress	Q86UA6.
Bgee	Q86UA6.
CleanEx	HS_RPAIN.
Genevestigator	Q86UA6.
GermOnline	ENSG00000129197. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
ChiTaRS	RPAIN. human.
GenomeRNAi	84268.
NextBio	73820.

Entry information

Entry name

RIP_HUMAN

Accession

Primary (citable) accession number: Q86UA6
Secondary accession number(s): B4DI36 , B4DTX7, E9PES3, Q4G2Y0, Q4G2Y5, Q4G2Y8, Q6B4V9, Q6B4W0, Q6B4W1, Q6B4W4, Q86X49, Q9BT00

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.