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Reviewed, UniProtKB/Swiss-Prot Q86U44 (MTA70_HUMAN)

Last modified January 19, 2010. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    N6-adenosine-methyltransferase 70 kDa subunit
      Short name=MT-A70
    EC=2.1.1.62
Alternative name(s):
    Methyltransferase-like protein 3
Gene names
Name: METTL3
Synonyms: MTA70
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. Ref.1

Catalytic activity

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

Subcellular location

Nucleus speckle. Note: Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components. Ref.1

Tissue specificity

Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Ref.1

Post-translational modification

Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.5 Ref.6 Ref.7 Ref.9 Ref.10

Sequence similarities

Belongs to the MT-A70-like family.

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Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processRNA methylation Ref.1

Inferred from mutant phenotype. Source: UniProtKB

   Cellular componentnuclear speck

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Ref.1

Inferred from mutant phenotype. Source: UniProtKB

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86U44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q86U44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-284: Missing.
     486-505: GVKGNPQGFNQGLDCDVIVA → SSSGAQFNRWSTKKNHLISY
     506-580: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580N6-adenosine-methyltransferase 70 kDa subunit
PRO_0000207630

Amino acid modifications

Modified residue431Phosphoserine Ref.5 Ref.7 Ref.9 Ref.10
Modified residue2191Phosphoserine Ref.7
Modified residue2431Phosphoserine Ref.7
Modified residue3501Phosphoserine Ref.6
Modified residue3561Phosphothreonine Ref.6

Natural variations

Alternative sequence1 – 284284Missing in isoform 2.
VSP_007864
Alternative sequence486 – 50520GVKGN…DVIVA → SSSGAQFNRWSTKKNHLISY in isoform 2.
VSP_007865
Alternative sequence506 – 58075Missing in isoform 2.
VSP_007866

Experimental info

Sequence conflict2511N → I in AAB71850. Ref.1
Sequence conflict263 – 2642KF → I in AAB71850. Ref.1
Sequence conflict3821D → V in AAB71850. Ref.1
Sequence conflict5681R → K in AAH52244. Ref.4

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 25, 2003. Version 2.
Checksum: 63A7F10195A3C6AC

FASTA58064,474
        10         20         30         40         50         60 
MSDTWSSIQA HKKQLDSLRE RLQRRRKQDS GHLDLRNPEA ALSPTFRSDS PVPTAPTSGG 

        70         80         90        100        110        120 
PKPSTASAVP ELATDPELEK KLLHHLSDLA LTLPTDAVSI CLAISTPDAP ATQDGVESLL 

       130        140        150        160        170        180 
QKFAAQELIE VKRGLLQDDA HPTLVTYADH SKLSAMMGAV AEKKGPGEVA GTVTGQKRRA 

       190        200        210        220        230        240 
EQDSTTVAAF ASSLVSGLNS SASEPAKEPA KKSRKHAASD VDLEIESLLN QQSTKEQQSK 

       250        260        270        280        290        300 
KVSQEILELL NTTTAKEQSI VEKFRSRGRA QVQEFCDYGT KEECMKASDA DRPCRKLHFR 

       310        320        330        340        350        360 
RIINKHTDES LGDCSFLNTC FHMDTCKYVH YEIDACMDSE APGSKDHTPS QELALTQSVG 

       370        380        390        400        410        420 
GDSSADRLFP PQWICCDIRY LDVSILGKFA VVMADPPWDI HMELPYGTLT DDEMRRLNIP 

       430        440        450        460        470        480 
VLQDDGFLFL WVTGRAMELG RECLNLWGYE RVDEIIWVKT NQLQRIIRTG RTGHWLNHGK 

       490        500        510        520        530        540 
EHCLVGVKGN PQGFNQGLDC DVIVAEVRST SHKPDEIYGM IERLSPGTRK IELFGRPHNV 

       550        560        570        580 
QPNWITLGNQ LDGIHLLDPD VVARFKQRYP DGIISKPKNL

« Hide

Isoform 2.

Checksum: D95A9087F7B99439
Show »

FASTA22125,479

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References

« Hide 'large scale' references
[1]"Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase."
Bokar J.A., Shambaugh M.E., Polayes D., Matera A.G., Rottman F.M.
RNA 3:1233-1247(1997) [PubMed: 9409616] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), PROTEIN SEQUENCE OF 48-56; 134-149 AND 509-522, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta.
[3]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed: 12508121] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung and Pancreas.
[5]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY.
Tissue: Epithelium.
[6]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350 AND THR-356, MASS SPECTROMETRY.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-219 AND SER-243, MASS SPECTROMETRY.
[8]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43, MASS SPECTROMETRY.
Tissue: T-cell.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF014837 Genomic DNA. Translation: AAB71850.1.
AF283991 Genomic DNA. Translation: AAG13956.1.
AE000658 Genomic DNA. No translation available.
BX247964 mRNA. Translation: CAD62303.1.
BC003031 mRNA. Translation: AAH03031.1.
BC001650 mRNA. Translation: AAH01650.1.
BC052244 mRNA. Translation: AAH52244.1.
IPIIPI00009755.
IPI00328636.
RefSeqNP_062826.2.
UniGeneHs.168799

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ86U44.

PTM databases

PhosphoSiteQ86U44.

Proteomic databases

PeptideAtlasQ86U44.
PRIDEQ86U44.

Genome annotation databases

EnsemblENST00000298717; ENSP00000298717; ENSG00000165819; Homo sapiens. [Genome view]
GeneID56339.
KEGGhsa:56339.
UCSCuc001wbc.1. human.

Organism-specific databases

CTD56339.
GeneCardsGC14M021036.
H-InvDBHIX0011511.
HGNCHGNC:17563. METTL3.
HPAHPA001299.
MIM612472. gene.
PharmGKBPA134955499.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG14482.
HOGENOMHBG738794.
HOVERGENQ86U44.
InParanoidQ86U44.
OMATKKSRKH.
OrthoDBEOG9NS5X9.
PhylomeDBQ86U44.

Enzyme and pathway databases

BRENDA2.1.1.62. 247.
ReactomeREACT_125. Processing of Capped Intron-Containing Pre-mRNA.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpressQ86U44.
BgeeQ86U44.
CleanExHS_METTL3.
GenevestigatorQ86U44.
GermOnlineENSG00000165819. Homo sapiens.

Family and domain databases

InterProIPR007757. MT-A70.
[Graphical view]
PfamPF05063. MT-A70. 1 hit.
[Graphical view]
PROSITEPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio61981.
SOURCESearch...

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Entry information

Entry nameMTA70_HUMAN
AccessionPrimary (citable) accession number: Q86U44
Secondary accession number(s): O14736, Q86V05, Q9HB32
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: July 25, 2003
Last modified: January 19, 2010
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents