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Q86U10 (LPP60_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60 kDa lysophospholipase

EC=3.1.1.5

Including the following 2 domains:

  1. L-asparaginase
    EC=3.5.1.1
    Alternative name(s):
    L-asparagine amidohydrolase
  2. Platelet-activating factor acetylhydrolase
    Short name=PAF acetylhydrolase
    EC=3.1.1.47
Gene names
Name:ASPG
Synonyms:C14orf76
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

L-asparagine + H2O = L-aspartate + NH3.

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Subunit structure

Monomer By similarity.

Sequence similarities

In the N-terminal section; belongs to the asparaginase 1 family.

Contains 5 ANK repeats.

Sequence caution

The sequence CAD62331.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q86U10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 3 (identifier: Q86U10-3)

The sequence of this isoform differs from the canonical sequence as follows:
     568-568: E → EPSCFQE
Note: May be due to a competing acceptor splice site.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 57357360 kDa lysophospholipase
PRO_0000324163

Regions

Repeat141 – 17030ANK 1
Repeat399 – 42931ANK 2
Repeat433 – 46230ANK 3
Repeat466 – 49530ANK 4
Repeat533 – 56230ANK 5
Region41 – 350310Asparaginase
Region84 – 863Substrate binding By similarity
Region116 – 1172Substrate binding By similarity

Sites

Active site191Acyl-ester intermediate By similarity

Natural variations

Alternative sequence5681E → EPSCFQE in isoform 3.
VSP_040740
Natural variant951C → R.
Corresponds to variant rs1770984 [ dbSNP | Ensembl ].
VAR_059131
Natural variant961L → V.
Corresponds to variant rs1744284 [ dbSNP | Ensembl ].
VAR_059132
Natural variant3441S → R. Ref.3
Corresponds to variant rs8012505 [ dbSNP | Ensembl ].
VAR_059133

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2011. Version 3.
Checksum: 62DBC583FF9C3AD7

FASTA57360,883
        10         20         30         40         50         60 
MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT 

        70         80         90        100        110        120 
LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA 

       130        140        150        160        170        180 
FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 

       190        200        210        220        230        240 
FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL 

       250        260        270        280        290        300 
LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL 

       310        320        330        340        350        360 
QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM 

       370        380        390        400        410        420 
TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL 

       430        440        450        460        470        480 
VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP 

       490        500        510        520        530        540 
GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV 

       550        560        570 
AEAAGNLAVV AFLQSLEGAV GAQAPCPEVL PGV 

« Hide

Isoform 3 [UniParc].

Checksum: 23E583A1518006CA
Show »

FASTA57961,575

References

[1]"The DNA sequence and analysis of human chromosome 14."
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. expand/collapse author list , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-344.
Tissue: Ovary and Testis.
[4]"Full-length cDNA libraries and normalization."
Li W.B., Gruber C., Jessee J., Polayes D.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-64.
Tissue: Fetal liver.
[5]The Cancer Genome Anatomy Project (CGAP) at the National Cancer Institute
Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 440-573 (ISOFORM 3).
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL136001 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81858.1.
CH471061 Genomic DNA. Translation: EAW81859.1.
BC035836 mRNA. Translation: AAH35836.1.
BC136637 mRNA. Translation: AAI36638.1.
BX247999 mRNA. Translation: CAD62331.1. Sequence problems.
AI373537 mRNA. No translation available.
CCDSCCDS45170.2. [Q86U10-1]
RefSeqNP_001073933.2. NM_001080464.2. [Q86U10-1]
XP_005267647.1. XM_005267590.2. [Q86U10-3]
UniGeneHs.146239.

3D structure databases

ProteinModelPortalQ86U10.
SMRQ86U10. Positions 8-362, 408-554.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000389003.

PTM databases

PhosphoSiteQ86U10.

Polymorphism databases

DMDM317373428.

Proteomic databases

PaxDbQ86U10.
PRIDEQ86U10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000455920; ENSP00000389003; ENSG00000166183. [Q86U10-3]
ENST00000551177; ENSP00000450040; ENSG00000166183. [Q86U10-1]
GeneID374569.
KEGGhsa:374569.
UCSCuc001yoo.2. human. [Q86U10-1]

Organism-specific databases

CTD374569.
GeneCardsGC14P104552.
HGNCHGNC:20123. ASPG.
neXtProtNX_Q86U10.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0666.
HOGENOMHOG000227974.
HOVERGENHBG079531.
KOK13278.
OMAIGMRSEL.
OrthoDBEOG7WDN22.
PhylomeDBQ86U10.
TreeFamTF315247.

Gene expression databases

ArrayExpressQ86U10.
BgeeQ86U10.
CleanExHS_ASPG.
GenevestigatorQ86U10.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamPF12796. Ank_2. 1 hit.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSPR01415. ANKYRIN.
PR00139. ASNGLNASE.
SMARTSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsTIGR00519. asnASE_I. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi374569.
NextBio100216.
PROQ86U10.

Entry information

Entry nameLPP60_HUMAN
AccessionPrimary (citable) accession number: Q86U10
Secondary accession number(s): B9EGQ2, Q8IV80
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 11, 2011
Last modified: July 9, 2014
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM