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Protein

60 kDa lysophospholipase

Gene

ASPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
L-asparagine + H2O = L-aspartate + NH3.
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS15413-MONOMER
ReactomeiR-HSA-70614 Amino acid synthesis and interconversion (transamination)

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa lysophospholipase (EC:3.1.1.5)
Including the following 2 domains:
L-asparaginase (EC:3.5.1.1)
Alternative name(s):
L-asparagine amidohydrolase
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Gene namesi
Name:ASPG
Synonyms:C14orf76
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000166183.15
HGNCiHGNC:20123 ASPG
neXtProtiNX_Q86U10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi374569
OpenTargetsiENSG00000166183

Polymorphism and mutation databases

BioMutaiASPG
DMDMi317373428

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241631 – 57360 kDa lysophospholipaseAdd BLAST573

Proteomic databases

PaxDbiQ86U10
PeptideAtlasiQ86U10
PRIDEiQ86U10

PTM databases

PhosphoSitePlusiQ86U10

Expressioni

Gene expression databases

BgeeiENSG00000166183
CleanExiHS_ASPG
ExpressionAtlasiQ86U10 baseline and differential
GenevisibleiQ86U10 HS

Organism-specific databases

HPAiHPA069761

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi131909, 1 interactor
STRINGi9606.ENSP00000450040

Structurei

3D structure databases

ProteinModelPortaliQ86U10
SMRiQ86U10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 355Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST347
Repeati141 – 170ANK 1Add BLAST30
Repeati399 – 429ANK 2Add BLAST31
Repeati433 – 462ANK 3Add BLAST30
Repeati466 – 495ANK 4Add BLAST30
Repeati533 – 562ANK 5Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 350AsparaginaseAdd BLAST310
Regioni84 – 86Substrate bindingBy similarity3
Regioni116 – 117Substrate bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the asparaginase 1 family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0503 Eukaryota
COG0252 LUCA
COG0666 LUCA
GeneTreeiENSGT00390000001610
HOGENOMiHOG000227974
HOVERGENiHBG079531
InParanoidiQ86U10
KOiK13278
OMAiYELENPI
OrthoDBiEOG091G0JYB
PhylomeDBiQ86U10
TreeFamiTF315247

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 2 hits
3.40.50.1170, 1 hit
3.40.50.40, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR006033 AsnASEI
IPR036152 Asp/glu_Ase-like_sf
IPR006034 Asparaginase/glutaminase-like
IPR027475 Asparaginase/glutaminase_AS2
IPR027473 L-asparaginase_C
IPR027474 L-asparaginase_N
IPR037152 L-asparaginase_N_sf
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00710 Asparaginase, 1 hit
PRINTSiPR01415 ANKYRIN
PR00139 ASNGLNASE
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SM00870 Asparaginase, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF53774 SSF53774, 1 hit
TIGRFAMsiTIGR00519 asnASE_I, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
PS00917 ASN_GLN_ASE_2, 1 hit
PS51732 ASN_GLN_ASE_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86U10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH
60 70 80 90 100
ARARGLSEDT LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI
110 120 130 140 150
KRHYEQYHGF VVIHGTDTMA FAASMLSFML ENLQKTVILT GAQVPIHALW
160 170 180 190 200
SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRATKVD ARRFAAFCSP
210 220 230 240 250
NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL LRLYPGIPAA
260 270 280 290 300
LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL
310 320 330 340 350
QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE
360 370 380 390 400
LLTKDLRGEM TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL
410 420 430 440 450
VPSLACAAAH AGDVEALQAL VELGSDLGLV DFNGQTPLHA AARGGHTEAV
460 470 480 490 500
TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP GVIGLLREAG ASLSTQELEE
510 520 530 540 550
AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV AEAAGNLAVV
560 570
AFLQSLEGAV GAQAPCPEVL PGV
Length:573
Mass (Da):60,883
Last modified:January 11, 2011 - v3
Checksum:i62DBC583FF9C3AD7
GO
Isoform 3 (identifier: Q86U10-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     568-568: E → EPSCFQE

Note: May be due to a competing acceptor splice site.
Show »
Length:579
Mass (Da):61,575
Checksum:i23E583A1518006CA
GO

Sequence cautioni

The sequence CAD62331 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05913195C → R. Corresponds to variant dbSNP:rs1770984Ensembl.1
Natural variantiVAR_05913296L → V. Corresponds to variant dbSNP:rs1744284Ensembl.1
Natural variantiVAR_059133344S → R1 PublicationCorresponds to variant dbSNP:rs8012505Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040740568E → EPSCFQE in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136001 Genomic DNA No translation available.
CH471061 Genomic DNA Translation: EAW81858.1
CH471061 Genomic DNA Translation: EAW81859.1
BC035836 mRNA Translation: AAH35836.1
BC136637 mRNA Translation: AAI36638.1
BX247999 mRNA Translation: CAD62331.1 Sequence problems.
AI373537 mRNA No translation available.
CCDSiCCDS45170.2 [Q86U10-1]
RefSeqiNP_001073933.2, NM_001080464.2 [Q86U10-1]
XP_005267647.1, XM_005267590.4 [Q86U10-3]
UniGeneiHs.146239

Genome annotation databases

EnsembliENST00000551177; ENSP00000450040; ENSG00000166183 [Q86U10-1]
GeneIDi374569
KEGGihsa:374569
UCSCiuc001yoq.3 human [Q86U10-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLPP60_HUMAN
AccessioniPrimary (citable) accession number: Q86U10
Secondary accession number(s): B9EGQ2, Q8IV80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 11, 2011
Last modified: March 28, 2018
This is version 119 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health