Q86U10 (LPP60_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60 kDa lysophospholipase
EC=3.1.1.5

Including the following 2 domains:

1. L-asparaginase
   EC=3.5.1.1
   Alternative name(s):
   L-asparagine amidohydrolase
2. Platelet-activating factor acetylhydrolase
   Short name=PAF acetylhydrolase
   EC=3.1.1.47

Gene names

Name:	ASPG
Synonyms:	C14orf76

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.
Catalytic activity	2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. L-asparagine + H2O = L-aspartate + NH3. 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Monomer By similarity.
Sequence similarities	In the N-terminal section; belongs to the asparaginase 1 family. Contains 5 ANK repeats.
Sequence caution	The sequence CAD62331.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	asparagine metabolic process Inferred from electronic annotation. Source: Compara lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: Compara
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC asparaginase activity Inferred from electronic annotation. Source: EC lysophospholipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86U10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 3 (identifier: Q86U10-3)

The sequence of this isoform differs from the canonical sequence as follows:
     568-568: E → EPSCFQE

Note: May be due to a competing acceptor splice site.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	60 kDa lysophospholipase		PRO_0000324163
Regions
	Repeat	141 – 170	30	ANK 1
	Repeat	399 – 429	31	ANK 2
	Repeat	433 – 462	30	ANK 3
	Repeat	466 – 495	30	ANK 4
	Repeat	533 – 562	30	ANK 5
	Region	41 – 350	310	Asparaginase
	Region	84 – 86	3	Substrate binding By similarity
	Region	116 – 117	2	Substrate binding By similarity
Sites
	Active site	19	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	390	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	568	1	E → EPSCFQE in isoform 3.		VSP_040740
	Natural variant	95	1	C → R. Corresponds to variant rs1770984 [ dbSNP | Ensembl ].		VAR_059131
	Natural variant	96	1	L → V. Corresponds to variant rs1744284 [ dbSNP | Ensembl ].		VAR_059132
	Natural variant	344	1	S → R. Ref.3 Corresponds to variant rs8012505 [ dbSNP | Ensembl ].		VAR_059133

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 11, 2011. Version 3. Checksum: 62DBC583FF9C3AD7	FASTA	573	60,883
	10 20 30 40 50 60 MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT 70 80 90 100 110 120 LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA 130 140 150 160 170 180 FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 190 200 210 220 230 240 FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL 250 260 270 280 290 300 LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL 310 320 330 340 350 360 QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM 370 380 390 400 410 420 TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL 430 440 450 460 470 480 VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP 490 500 510 520 530 540 GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV 550 560 570 AEAAGNLAVV AFLQSLEGAV GAQAPCPEVL PGV
	Isoform 3 [UniParc]. Checksum: 23E583A1518006CA	FASTA	579	61,575
	10 20 30 40 50 60 MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT 70 80 90 100 110 120 LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA 130 140 150 160 170 180 FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 190 200 210 220 230 240 FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL 250 260 270 280 290 300 LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL 310 320 330 340 350 360 QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM 370 380 390 400 410 420 TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL 430 440 450 460 470 480 VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP 490 500 510 520 530 540 GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV 550 560 570 AEAAGNLAVV AFLQSLEGAV GAQAPCPEPS CFQEVLPGV

References

[1]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-344. Tissue: Ovary and Testis.
[4]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-64. Tissue: Fetal liver.
[5]	The Cancer Genome Anatomy Project (CGAP) at the National Cancer Institute Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 440-573 (ISOFORM 3). Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL136001 Genomic DNA. No translation available. CH471061 Genomic DNA. Translation: EAW81858.1. CH471061 Genomic DNA. Translation: EAW81859.1. BC035836 mRNA. Translation: AAH35836.1. BC136637 mRNA. Translation: AAI36638.1. BX247999 mRNA. Translation: CAD62331.1. Sequence problems. AI373537 mRNA. No translation available.
IPI	IPI00735641. IPI00985438.
RefSeq	NP_001073933.2. NM_001080464.2.
UniGene	Hs.146239.
3D structure databases
HSSP	HSSP built from PDB template 1ZQ1 based on UniProtKB Q9V0T9.
ProteinModelPortal	Q86U10.
SMR	Q86U10. Positions 8-553.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000389003.
PTM databases
PhosphoSite	Q86U10.
Polymorphism databases
DMDM	172046698.
Proteomic databases
PaxDb	Q86U10.
PRIDE	Q86U10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000455920; ENSP00000389003; ENSG00000166183. ENST00000551177; ENSP00000450040; ENSG00000166183.
GeneID	374569.
KEGG	hsa:374569.
UCSC	uc001yop.2. human. uc001yoq.2. human.
Organism-specific databases
CTD	374569.
GeneCards	GC14P104552.
HGNC	HGNC:20123. ASPG.
neXtProt	NX_Q86U10.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000227974.
HOVERGEN	HBG079531.
KO	K13278.
OMA	GTIGMRS.
OrthoDB	EOG4RR6H4.
Gene expression databases
ArrayExpress	Q86U10.
Bgee	Q86U10.
CleanEx	HS_ASPG.
Genevestigator	Q86U10.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR006033. AsnASEI. IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
Pfam	PF12796. Ank_2. 1 hit. PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN. PR00139. ASNGLNASE.
SMART	SM00248. ANK. 4 hits. SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF53774. Asp/Glutamnse. 1 hit.
TIGRFAMs	TIGR00519. asnASE_I. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS00144. ASN_GLN_ASE_1. False negative. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	374569.
NextBio	100216.

Entry information

Entry name

LPP60_HUMAN

Accession

Primary (citable) accession number: Q86U10
Secondary accession number(s): B9EGQ2, Q8IV80

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 11, 2011
Last modified:	May 1, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.