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Protein

60 kDa lysophospholipase

Gene

ASPG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
L-asparagine + H2O = L-aspartate + NH3.
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS15413-MONOMER.
ZFISH:HS15413-MONOMER.
ReactomeiR-HSA-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa lysophospholipase (EC:3.1.1.5)
Including the following 2 domains:
L-asparaginase (EC:3.5.1.1)
Alternative name(s):
L-asparagine amidohydrolase
Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
Short name:
PAF acetylhydrolase
Gene namesi
Name:ASPG
Synonyms:C14orf76
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20123. ASPG.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi374569.
OpenTargetsiENSG00000166183.

Polymorphism and mutation databases

BioMutaiASPG.
DMDMi317373428.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241631 – 57360 kDa lysophospholipaseAdd BLAST573

Proteomic databases

PaxDbiQ86U10.
PeptideAtlasiQ86U10.
PRIDEiQ86U10.

PTM databases

PhosphoSitePlusiQ86U10.

Expressioni

Gene expression databases

BgeeiENSG00000166183.
CleanExiHS_ASPG.
ExpressionAtlasiQ86U10. baseline and differential.
GenevisibleiQ86U10. HS.

Organism-specific databases

HPAiHPA069761.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9606.ENSP00000450040.

Structurei

3D structure databases

ProteinModelPortaliQ86U10.
SMRiQ86U10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 355Asparaginase/glutaminasePROSITE-ProRule annotationAdd BLAST347
Repeati141 – 170ANK 1Add BLAST30
Repeati399 – 429ANK 2Add BLAST31
Repeati433 – 462ANK 3Add BLAST30
Repeati466 – 495ANK 4Add BLAST30
Repeati533 – 562ANK 5Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 350AsparaginaseAdd BLAST310
Regioni84 – 86Substrate bindingBy similarity3
Regioni116 – 117Substrate bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the asparaginase 1 family.Curated
Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 1 asparaginase/glutaminase domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0503. Eukaryota.
COG0252. LUCA.
COG0666. LUCA.
GeneTreeiENSGT00390000001610.
HOGENOMiHOG000227974.
HOVERGENiHBG079531.
InParanoidiQ86U10.
KOiK13278.
OMAiIGMRSEL.
OrthoDBiEOG091G0JYB.
PhylomeDBiQ86U10.
TreeFamiTF315247.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR01415. ANKYRIN.
PR00139. ASNGLNASE.
SMARTiSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q86U10-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH
60 70 80 90 100
ARARGLSEDT LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI
110 120 130 140 150
KRHYEQYHGF VVIHGTDTMA FAASMLSFML ENLQKTVILT GAQVPIHALW
160 170 180 190 200
SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRATKVD ARRFAAFCSP
210 220 230 240 250
NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL LRLYPGIPAA
260 270 280 290 300
LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL
310 320 330 340 350
QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE
360 370 380 390 400
LLTKDLRGEM TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL
410 420 430 440 450
VPSLACAAAH AGDVEALQAL VELGSDLGLV DFNGQTPLHA AARGGHTEAV
460 470 480 490 500
TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP GVIGLLREAG ASLSTQELEE
510 520 530 540 550
AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV AEAAGNLAVV
560 570
AFLQSLEGAV GAQAPCPEVL PGV
Length:573
Mass (Da):60,883
Last modified:January 11, 2011 - v3
Checksum:i62DBC583FF9C3AD7
GO
Isoform 3 (identifier: Q86U10-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     568-568: E → EPSCFQE

Note: May be due to a competing acceptor splice site.
Show »
Length:579
Mass (Da):61,575
Checksum:i23E583A1518006CA
GO

Sequence cautioni

The sequence CAD62331 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05913195C → R.Corresponds to variant rs1770984dbSNPEnsembl.1
Natural variantiVAR_05913296L → V.Corresponds to variant rs1744284dbSNPEnsembl.1
Natural variantiVAR_059133344S → R.1 PublicationCorresponds to variant rs8012505dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040740568E → EPSCFQE in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136001 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81858.1.
CH471061 Genomic DNA. Translation: EAW81859.1.
BC035836 mRNA. Translation: AAH35836.1.
BC136637 mRNA. Translation: AAI36638.1.
BX247999 mRNA. Translation: CAD62331.1. Sequence problems.
AI373537 mRNA. No translation available.
CCDSiCCDS45170.2. [Q86U10-1]
RefSeqiNP_001073933.2. NM_001080464.2. [Q86U10-1]
XP_005267647.1. XM_005267590.4. [Q86U10-3]
UniGeneiHs.146239.

Genome annotation databases

EnsembliENST00000551177; ENSP00000450040; ENSG00000166183. [Q86U10-1]
GeneIDi374569.
KEGGihsa:374569.
UCSCiuc001yoq.3. human. [Q86U10-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136001 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW81858.1.
CH471061 Genomic DNA. Translation: EAW81859.1.
BC035836 mRNA. Translation: AAH35836.1.
BC136637 mRNA. Translation: AAI36638.1.
BX247999 mRNA. Translation: CAD62331.1. Sequence problems.
AI373537 mRNA. No translation available.
CCDSiCCDS45170.2. [Q86U10-1]
RefSeqiNP_001073933.2. NM_001080464.2. [Q86U10-1]
XP_005267647.1. XM_005267590.4. [Q86U10-3]
UniGeneiHs.146239.

3D structure databases

ProteinModelPortaliQ86U10.
SMRiQ86U10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000450040.

PTM databases

PhosphoSitePlusiQ86U10.

Polymorphism and mutation databases

BioMutaiASPG.
DMDMi317373428.

Proteomic databases

PaxDbiQ86U10.
PeptideAtlasiQ86U10.
PRIDEiQ86U10.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000551177; ENSP00000450040; ENSG00000166183. [Q86U10-1]
GeneIDi374569.
KEGGihsa:374569.
UCSCiuc001yoq.3. human. [Q86U10-1]

Organism-specific databases

CTDi374569.
DisGeNETi374569.
GeneCardsiASPG.
HGNCiHGNC:20123. ASPG.
HPAiHPA069761.
neXtProtiNX_Q86U10.
OpenTargetsiENSG00000166183.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0503. Eukaryota.
COG0252. LUCA.
COG0666. LUCA.
GeneTreeiENSGT00390000001610.
HOGENOMiHOG000227974.
HOVERGENiHBG079531.
InParanoidiQ86U10.
KOiK13278.
OMAiIGMRSEL.
OrthoDBiEOG091G0JYB.
PhylomeDBiQ86U10.
TreeFamiTF315247.

Enzyme and pathway databases

BioCyciMetaCyc:HS15413-MONOMER.
ZFISH:HS15413-MONOMER.
ReactomeiR-HSA-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

GenomeRNAii374569.
PROiQ86U10.

Gene expression databases

BgeeiENSG00000166183.
CleanExiHS_ASPG.
ExpressionAtlasiQ86U10. baseline and differential.
GenevisibleiQ86U10. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.40.50.1170. 1 hit.
3.40.50.40. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR006033. AsnASEI.
IPR006034. Asparaginase/glutaminase.
IPR027475. Asparaginase/glutaminase_AS2.
IPR027473. L-asparaginase_C.
IPR027474. L-asparaginase_N.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00710. Asparaginase. 1 hit.
[Graphical view]
PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
PRINTSiPR01415. ANKYRIN.
PR00139. ASNGLNASE.
SMARTiSM00248. ANK. 4 hits.
SM00870. Asparaginase. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF53774. SSF53774. 1 hit.
TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS00917. ASN_GLN_ASE_2. 1 hit.
PS51732. ASN_GLN_ASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPP60_HUMAN
AccessioniPrimary (citable) accession number: Q86U10
Secondary accession number(s): B9EGQ2, Q8IV80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: January 11, 2011
Last modified: November 2, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.