Reviewed, UniProtKB/Swiss-Prot Q86U10 (LPP60_HUMAN)

Names and origin

Protein names

Recommended name:
    60 kDa lysophospholipase
    EC=3.1.1.5
Including the following 2 domains:
    1- Recommended name:
            L-asparaginase
              EC=3.5.1.1
        Alternative name(s):
            L-asparagine amidohydrolase
    2- Recommended name:
            Platelet-activating factor acetylhydrolase
                Short name=PAF acetylhydrolase
              EC=3.1.1.47

Gene names

Name:

ASPG

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.
Catalytic activity	2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate. L-asparagine + H2O = L-aspartate + NH3. 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Monomer By similarity.
Sequence similarities	In the N-terminal section; belongs to the asparaginase 1 family. Contains 5 ANK repeats.

Ontologies

Keywords
Biological process	Lipid degradation
Coding sequence diversity	Alternative splicing Polymorphism
Domain	ANK repeat Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC asparaginase activity Inferred from electronic annotation. Source: EC lysophospholipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86U10-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86U10-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: MARAVGPERR...GLSEDTLVLP → MAGPSPPLRA...FRLLLFLSRS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	60 kDa lysophospholipase		PRO_0000324163
Regions
	Repeat	141 – 170	30	ANK 1
	Repeat	399 – 429	31	ANK 2
	Repeat	433 – 462	30	ANK 3
	Repeat	466 – 495	30	ANK 4
	Repeat	533 – 562	30	ANK 5
	Region	41 – 350	310	Asparaginase
Sites
	Active site	19	1	By similarity
	Active site	116	1	By similarity
	Active site	117	1	By similarity
	Active site	187	1	By similarity
Amino acid modifications
	Modified residue	390	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 64	64	MARAV…TLVLP → MAGPSPPLRAGVGRRLLGVA HPDLRPCLCGELQVEGGPGP VPGMLRTCYVRSPWRRGPSS ARGEQSGSGHLEQEEVQSGS LLFRLLLFLSRS in isoform 2.		VSP_032165
	Natural variant	95	1	C → R: dbSNP rs1770984.		VAR_059131
	Natural variant	96	1	L → V: dbSNP rs1744284.		VAR_059132
	Natural variant	344	1	S → R: dbSNP rs8012505.		VAR_059133

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 6AD71C6F27B15DDE	FASTA	567	60,288
	10 20 30 40 50 60 MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH ARARGLSEDT 70 80 90 100 110 120 LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI KRHYEQYHGF VVIHGTDTMA 130 140 150 160 170 180 FAASMLSFML ENLQKTVILT GAQVPIHALW SDGRENLLGA LLMAGQYVIP EVCLFFQNQL 190 200 210 220 230 240 FRGNRATKVD ARRFAAFCSP NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL 250 260 270 280 290 300 LRLYPGIPAA LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL 310 320 330 340 350 360 QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE LLTKDLRGEM 370 380 390 400 410 420 TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL VPSLACAAAH AGDVEALQAL 430 440 450 460 470 480 VELGSDLGLV DFNGQTPLHA AARGGHTEAV TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP 490 500 510 520 530 540 GVIGLLREAG ASLSTQELEE AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV 550 560 AEAAGNLAVV AFLQSLEGAV GAQAPCP
	Isoform 2. Checksum: 938C7A4F8C6A101C	FASTA	595	63,281
	10 20 30 40 50 60 MAGPSPPLRA GVGRRLLGVA HPDLRPCLCG ELQVEGGPGP VPGMLRTCYV RSPWRRGPSS 70 80 90 100 110 120 ARGEQSGSGH LEQEEVQSGS LLFRLLLFLS RSPASRNQRI LYTVLECQPL FDSSDMTIAE 130 140 150 160 170 180 WVCLAQTIKR HYEQYHGFVV IHGTDTMAFA ASMLSFMLEN LQKTVILTGA QVPIHALWSD 190 200 210 220 230 240 GRENLLGALL MAGQYVIPEV CLFFQNQLFR GNRATKVDAR RFAAFCSPNL LPLATVGADI 250 260 270 280 290 300 TINRELVRKV DGKAGLVVHS SMEQDVGLLR LYPGIPAALV RAFLQPPLKG VVMETFGSGN 310 320 330 340 350 360 GPTKPDLLQE LRVATERGLV IVNCTHCLQG AVTTDYAAGM AMAGAGVISG FDMTSEAALA 370 380 390 400 410 420 KLSYVLGQPG LSLDVRKELL TKDLRGEMTP PSVEERRPSL QGNTLGGGVS WLLSLSGSQE 430 440 450 460 470 480 ADALRNALVP SLACAAAHAG DVEALQALVE LGSDLGLVDF NGQTPLHAAA RGGHTEAVTM 490 500 510 520 530 540 LLQRGVDVNT RDTDGFSPLL LAVRGRHPGV IGLLREAGAS LSTQELEEAG TELCRLAYRA 550 560 570 580 590 DLEGLQVWWQ AGADLGQPGY DGHSALHVAE AAGNLAVVAF LQSLEGAVGA QAPCP

References

[1]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed: 12508121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-441 (ISOFORM 2). Tissue: Fetal liver.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-441.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-439 (ISOFORM 1). Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL136001 Genomic DNA. No translation available. BX247999 mRNA. Translation: CAD62331.1. CH471061 Genomic DNA. Translation: EAW81858.1. BC035836 mRNA. Translation: AAH35836.1.
IPI	IPI00384125. IPI00735641.
UniGene	Hs.146239
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q86U10.
PTM databases
PhosphoSite	Q86U10.
Genome annotation databases
Ensembl	ENST00000299234; ENSP00000299234; ENSG00000166183; Homo sapiens. [Genome view] ENST00000455920; ENSP00000389003; ENSG00000166183; Homo sapiens. [Genome view]
UCSC	uc001yoo.1. human. uc001yop.1. human.
Organism-specific databases
GeneCards	GC14P103622.
HGNC	HGNC:20123. ASPG.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q86U10.
Enzyme and pathway databases
BRENDA	3.1.1.47. 247. 3.1.1.5. 247. 3.5.1.1. 247.
Gene expression databases
Bgee	Q86U10.
CleanEx	HS_ASPG.
Genevestigator	Q86U10.
Family and domain databases
InterPro	IPR002110. ANK. IPR006033. AsnASEI. IPR006034. Asp/Glutamnse. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00023. Ank. 4 hits. PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00248. ANK. 4 hits. [Graphical view]
TIGRFAMs	TIGR00519. asnASE_I. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS00144. ASN_GLN_ASE_1. False negative. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPP60_HUMAN

Accession

Primary (citable) accession number: Q86U10
Secondary accession number(s): Q8IV80

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	November 3, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.