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Q86U10

- LPP60_HUMAN

UniProt

Q86U10 - LPP60_HUMAN

Protein

60 kDa lysophospholipase

Gene

ASPG

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 3 (11 Jan 2011)
      Previous versions | rss
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    Functioni

    Exhibits lysophospholipase, transacylase, PAF acetylhydrolase and asparaginase activities.

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    L-asparagine + H2O = L-aspartate + NH3.
    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: UniProtKB-EC
    2. asparaginase activity Source: UniProtKB-EC
    3. lysophospholipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. asparagine metabolic process Source: Ensembl
    2. lipid catabolic process Source: UniProtKB-KW
    3. phospholipid metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60 kDa lysophospholipase (EC:3.1.1.5)
    Including the following 2 domains:
    L-asparaginase (EC:3.5.1.1)
    Alternative name(s):
    L-asparagine amidohydrolase
    Platelet-activating factor acetylhydrolase (EC:3.1.1.47)
    Short name:
    PAF acetylhydrolase
    Gene namesi
    Name:ASPG
    Synonyms:C14orf76
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:20123. ASPG.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 57357360 kDa lysophospholipasePRO_0000324163Add
    BLAST

    Proteomic databases

    PaxDbiQ86U10.
    PRIDEiQ86U10.

    PTM databases

    PhosphoSiteiQ86U10.

    Expressioni

    Gene expression databases

    ArrayExpressiQ86U10.
    BgeeiQ86U10.
    CleanExiHS_ASPG.
    GenevestigatoriQ86U10.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9606.ENSP00000389003.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86U10.
    SMRiQ86U10. Positions 8-362, 408-554.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati141 – 17030ANK 1Add
    BLAST
    Repeati399 – 42931ANK 2Add
    BLAST
    Repeati433 – 46230ANK 3Add
    BLAST
    Repeati466 – 49530ANK 4Add
    BLAST
    Repeati533 – 56230ANK 5Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 350310AsparaginaseAdd
    BLAST
    Regioni84 – 863Substrate bindingBy similarity
    Regioni116 – 1172Substrate bindingBy similarity

    Sequence similaritiesi

    In the N-terminal section; belongs to the asparaginase 1 family.Curated
    Contains 5 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiCOG0666.
    HOGENOMiHOG000227974.
    HOVERGENiHBG079531.
    KOiK13278.
    OMAiIGMRSEL.
    OrthoDBiEOG7WDN22.
    PhylomeDBiQ86U10.
    TreeFamiTF315247.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    3.40.50.1170. 1 hit.
    3.40.50.40. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR006033. AsnASEI.
    IPR006034. Asparaginase/glutaminase.
    IPR027475. Asparaginase/glutaminase_AS2.
    IPR027473. L-asparaginase_C.
    IPR027474. L-asparaginase_N.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF00710. Asparaginase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001220. L-ASNase_gatD. 1 hit.
    PRINTSiPR01415. ANKYRIN.
    PR00139. ASNGLNASE.
    SMARTiSM00248. ANK. 4 hits.
    SM00870. Asparaginase. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF53774. SSF53774. 1 hit.
    TIGRFAMsiTIGR00519. asnASE_I. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS00917. ASN_GLN_ASE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q86U10-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARAVGPERR LLAVYTGGTI GMRSELGVLV PGTGLAAILR TLPMFHDEEH    50
    ARARGLSEDT LVLPPASRNQ RILYTVLECQ PLFDSSDMTI AEWVCLAQTI 100
    KRHYEQYHGF VVIHGTDTMA FAASMLSFML ENLQKTVILT GAQVPIHALW 150
    SDGRENLLGA LLMAGQYVIP EVCLFFQNQL FRGNRATKVD ARRFAAFCSP 200
    NLLPLATVGA DITINRELVR KVDGKAGLVV HSSMEQDVGL LRLYPGIPAA 250
    LVRAFLQPPL KGVVMETFGS GNGPTKPDLL QELRVATERG LVIVNCTHCL 300
    QGAVTTDYAA GMAMAGAGVI SGFDMTSEAA LAKLSYVLGQ PGLSLDVRKE 350
    LLTKDLRGEM TPPSVEERRP SLQGNTLGGG VSWLLSLSGS QEADALRNAL 400
    VPSLACAAAH AGDVEALQAL VELGSDLGLV DFNGQTPLHA AARGGHTEAV 450
    TMLLQRGVDV NTRDTDGFSP LLLAVRGRHP GVIGLLREAG ASLSTQELEE 500
    AGTELCRLAY RADLEGLQVW WQAGADLGQP GYDGHSALHV AEAAGNLAVV 550
    AFLQSLEGAV GAQAPCPEVL PGV 573
    Length:573
    Mass (Da):60,883
    Last modified:January 11, 2011 - v3
    Checksum:i62DBC583FF9C3AD7
    GO
    Isoform 3 (identifier: Q86U10-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         568-568: E → EPSCFQE

    Note: May be due to a competing acceptor splice site.

    Show »
    Length:579
    Mass (Da):61,575
    Checksum:i23E583A1518006CA
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti95 – 951C → R.
    Corresponds to variant rs1770984 [ dbSNP | Ensembl ].
    VAR_059131
    Natural varianti96 – 961L → V.
    Corresponds to variant rs1744284 [ dbSNP | Ensembl ].
    VAR_059132
    Natural varianti344 – 3441S → R.1 Publication
    Corresponds to variant rs8012505 [ dbSNP | Ensembl ].
    VAR_059133

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei568 – 5681E → EPSCFQE in isoform 3. 1 PublicationVSP_040740

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL136001 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW81858.1.
    CH471061 Genomic DNA. Translation: EAW81859.1.
    BC035836 mRNA. Translation: AAH35836.1.
    BC136637 mRNA. Translation: AAI36638.1.
    BX247999 mRNA. Translation: CAD62331.1. Sequence problems.
    AI373537 mRNA. No translation available.
    CCDSiCCDS45170.2. [Q86U10-1]
    RefSeqiNP_001073933.2. NM_001080464.2. [Q86U10-1]
    XP_005267647.1. XM_005267590.2. [Q86U10-3]
    UniGeneiHs.146239.

    Genome annotation databases

    EnsembliENST00000551177; ENSP00000450040; ENSG00000166183. [Q86U10-1]
    GeneIDi374569.
    KEGGihsa:374569.
    UCSCiuc001yoo.2. human. [Q86U10-1]

    Polymorphism databases

    DMDMi317373428.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL136001 Genomic DNA. No translation available.
    CH471061 Genomic DNA. Translation: EAW81858.1 .
    CH471061 Genomic DNA. Translation: EAW81859.1 .
    BC035836 mRNA. Translation: AAH35836.1 .
    BC136637 mRNA. Translation: AAI36638.1 .
    BX247999 mRNA. Translation: CAD62331.1 . Sequence problems.
    AI373537 mRNA. No translation available.
    CCDSi CCDS45170.2. [Q86U10-1 ]
    RefSeqi NP_001073933.2. NM_001080464.2. [Q86U10-1 ]
    XP_005267647.1. XM_005267590.2. [Q86U10-3 ]
    UniGenei Hs.146239.

    3D structure databases

    ProteinModelPortali Q86U10.
    SMRi Q86U10. Positions 8-362, 408-554.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000389003.

    PTM databases

    PhosphoSitei Q86U10.

    Polymorphism databases

    DMDMi 317373428.

    Proteomic databases

    PaxDbi Q86U10.
    PRIDEi Q86U10.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000551177 ; ENSP00000450040 ; ENSG00000166183 . [Q86U10-1 ]
    GeneIDi 374569.
    KEGGi hsa:374569.
    UCSCi uc001yoo.2. human. [Q86U10-1 ]

    Organism-specific databases

    CTDi 374569.
    GeneCardsi GC14P104552.
    HGNCi HGNC:20123. ASPG.
    neXtProti NX_Q86U10.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0666.
    HOGENOMi HOG000227974.
    HOVERGENi HBG079531.
    KOi K13278.
    OMAi IGMRSEL.
    OrthoDBi EOG7WDN22.
    PhylomeDBi Q86U10.
    TreeFami TF315247.

    Miscellaneous databases

    GenomeRNAii 374569.
    NextBioi 100216.
    PROi Q86U10.

    Gene expression databases

    ArrayExpressi Q86U10.
    Bgeei Q86U10.
    CleanExi HS_ASPG.
    Genevestigatori Q86U10.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    3.40.50.1170. 1 hit.
    3.40.50.40. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR006033. AsnASEI.
    IPR006034. Asparaginase/glutaminase.
    IPR027475. Asparaginase/glutaminase_AS2.
    IPR027473. L-asparaginase_C.
    IPR027474. L-asparaginase_N.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF00710. Asparaginase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001220. L-ASNase_gatD. 1 hit.
    PRINTSi PR01415. ANKYRIN.
    PR00139. ASNGLNASE.
    SMARTi SM00248. ANK. 4 hits.
    SM00870. Asparaginase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF53774. SSF53774. 1 hit.
    TIGRFAMsi TIGR00519. asnASE_I. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 2 hits.
    PS00917. ASN_GLN_ASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence and analysis of human chromosome 14."
      Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
      , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
      Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ARG-344.
      Tissue: Ovary and Testis.
    4. "Full-length cDNA libraries and normalization."
      Li W.B., Gruber C., Jessee J., Polayes D.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-64.
      Tissue: Fetal liver.
    5. The Cancer Genome Anatomy Project (CGAP) at the National Cancer Institute
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 440-573 (ISOFORM 3).
      Tissue: Kidney.

    Entry informationi

    Entry nameiLPP60_HUMAN
    AccessioniPrimary (citable) accession number: Q86U10
    Secondary accession number(s): B9EGQ2, Q8IV80
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 92 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3