Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acyl-coenzyme A thioesterase 1

Gene

ACOT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + H2O = CoA + palmitate.

Kineticsi

  1. KM=35.8 µM for C10-acyl-CoA1 Publication
  2. KM=3.6 µM for C12-acyl-CoA1 Publication
  3. KM=2.8 µM for C14-acyl-CoA1 Publication
  4. KM=3.6 µM for C16-acyl-CoA1 Publication
  5. KM=2.4 µM for C18-acyl-CoA1 Publication
  6. KM=2 µM for C20-acyl-CoA1 Publication
  7. KM=2.4 µM for C16:1-acyl-CoA1 Publication
  8. KM=4.1 µM for C18:1-acyl-CoA1 Publication
  9. KM=2.1 µM for C18:1-trans-acyl-CoA1 Publication

Vmax=224 nmol/min/mg enzyme toward C10-acyl-CoA1 Publication

Vmax=700 nmol/min/mg enzyme toward C12-acyl-CoA1 Publication

Vmax=912 nmol/min/mg enzyme toward C14-acyl-CoA1 Publication

Vmax=691 nmol/min/mg enzyme toward C16-acyl-CoA1 Publication

Vmax=597 nmol/min/mg enzyme toward C18-acyl-CoA1 Publication

Vmax=520 nmol/min/mg enzyme toward C20-acyl-CoA1 Publication

Vmax=577 nmol/min/mg enzyme toward C16:1-acyl-CoA1 Publication

Vmax=258 nmol/min/mg enzyme toward C18:1-acyl-CoA1 Publication

Vmax=309 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei232 – 2321Charge relay systemBy similarity
Active sitei326 – 3261Charge relay systemBy similarity
Active sitei360 – 3601Charge relay systemBy similarity

GO - Molecular functioni

  1. acyl-CoA hydrolase activity Source: HGNC
  2. carboxylic ester hydrolase activity Source: UniProtKB-KW
  3. palmitoyl-CoA hydrolase activity Source: UniProtKB-EC

GO - Biological processi

  1. acyl-CoA metabolic process Source: HGNC
  2. long-chain fatty acid metabolic process Source: HGNC
  3. very long-chain fatty acid metabolic process Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14105.
BRENDAi3.1.2.2. 2681.
SABIO-RKQ86TX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A thioesterase 1 (EC:3.1.2.2)
Short name:
Acyl-CoA thioesterase 1
Alternative name(s):
CTE-I
CTE-Ib
Inducible cytosolic acyl-coenzyme A thioester hydrolase
Long chain acyl-CoA thioester hydrolase
Short name:
Long chain acyl-CoA hydrolase
Gene namesi
Name:ACOT1
Synonyms:CTE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:33128. ACOT1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162375318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 421420Acyl-coenzyme A thioesterase 1PRO_0000202155Add
BLAST

Proteomic databases

MaxQBiQ86TX2.
PaxDbiQ86TX2.
PRIDEiQ86TX2.

2D gel databases

UCD-2DPAGEQ86TX2.

PTM databases

PhosphoSiteiQ86TX2.

Expressioni

Gene expression databases

BgeeiQ86TX2.
CleanExiHS_ACOT1.
ExpressionAtlasiQ86TX2. baseline.
GenevestigatoriQ86TX2.

Organism-specific databases

HPAiHPA043705.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi534965. 6 interactions.
STRINGi9606.ENSP00000311224.

Structurei

3D structure databases

ProteinModelPortaliQ86TX2.
SMRiQ86TX2. Positions 1-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the C/M/P thioester hydrolase family.Curated

Phylogenomic databases

eggNOGiCOG1073.
GeneTreeiENSGT00390000001046.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiQ86TX2.
KOiK01068.
OMAiTRTEIEY.
OrthoDBiEOG75TMC9.
PhylomeDBiQ86TX2.
TreeFamiTF314911.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q86TX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATLILEPA GRCCWDEPVR IAVRGLAPEQ PVTLRASLRD EKGALFQAHA
60 70 80 90 100
RYRADTLGEL DLERAPALGG SFAGLEPMGL LWALEPEKPL VRLVKRDVRT
110 120 130 140 150
PLAVELEVLD GHDPDPGRLL CRVRHERYFL PPGVRREPVR AGRVRGTLFL
160 170 180 190 200
PPEPGPFPGI VDMFGTGGGL LEYRASLLAG KGFAVMALAY YNYEDLPKTM
210 220 230 240 250
ETLHLEYFEE AVNYLLSHPE VKGPGVGLLG ISKGGELCLS MASFLKGITA
260 270 280 290 300
AVVINGSVAN VGGTLRYKGE TLPPVGVNRN RIKVTKDGYA DIVDVLNSPL
310 320 330 340 350
EGPDQKSFIP VERAESTFLF LVGQDDHNWK SEFYANEACK RLQAHGRRKP
360 370 380 390 400
QIICYPETGH YIEPPYFPLC RASLHALVGS PIIWGGEPRA HAMAQVDAWK
410 420
QLQTFFHKHL GGHEGTIPSK V
Length:421
Mass (Da):46,277
Last modified:June 1, 2003 - v1
Checksum:i04675F28D9D53B97
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti266 – 2661R → H.
Corresponds to variant rs1049568 [ dbSNP | Ensembl ].
VAR_059830

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ082754 mRNA. Translation: AAZ31236.1.
BX161396 mRNA. Translation: CAD61883.1.
BC127748 mRNA. Translation: AAI27749.1.
BC132889 mRNA. Translation: AAI32890.1.
BC132891 mRNA. Translation: AAI32892.1.
BC143042 mRNA. Translation: AAI43043.1.
CCDSiCCDS32117.1.
RefSeqiNP_001032238.1. NM_001037161.1.
UniGeneiHs.446685.
Hs.568046.

Genome annotation databases

EnsembliENST00000311148; ENSP00000311224; ENSG00000184227.
GeneIDi641371.
KEGGihsa:641371.
UCSCiuc001xol.1. human.

Polymorphism databases

DMDMi50428913.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ082754 mRNA. Translation: AAZ31236.1.
BX161396 mRNA. Translation: CAD61883.1.
BC127748 mRNA. Translation: AAI27749.1.
BC132889 mRNA. Translation: AAI32890.1.
BC132891 mRNA. Translation: AAI32892.1.
BC143042 mRNA. Translation: AAI43043.1.
CCDSiCCDS32117.1.
RefSeqiNP_001032238.1. NM_001037161.1.
UniGeneiHs.446685.
Hs.568046.

3D structure databases

ProteinModelPortaliQ86TX2.
SMRiQ86TX2. Positions 1-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi534965. 6 interactions.
STRINGi9606.ENSP00000311224.

Chemistry

ChEMBLiCHEMBL2189136.

PTM databases

PhosphoSiteiQ86TX2.

Polymorphism databases

DMDMi50428913.

2D gel databases

UCD-2DPAGEQ86TX2.

Proteomic databases

MaxQBiQ86TX2.
PaxDbiQ86TX2.
PRIDEiQ86TX2.

Protocols and materials databases

DNASUi641371.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000311148; ENSP00000311224; ENSG00000184227.
GeneIDi641371.
KEGGihsa:641371.
UCSCiuc001xol.1. human.

Organism-specific databases

CTDi641371.
GeneCardsiGC14P074003.
HGNCiHGNC:33128. ACOT1.
HPAiHPA043705.
MIMi614313. gene.
neXtProtiNX_Q86TX2.
PharmGKBiPA162375318.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1073.
GeneTreeiENSGT00390000001046.
HOGENOMiHOG000116219.
HOVERGENiHBG000331.
InParanoidiQ86TX2.
KOiK01068.
OMAiTRTEIEY.
OrthoDBiEOG75TMC9.
PhylomeDBiQ86TX2.
TreeFamiTF314911.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14105.
BRENDAi3.1.2.2. 2681.
SABIO-RKQ86TX2.

Miscellaneous databases

ChiTaRSiACOT1. human.
GenomeRNAii641371.
NextBioi113125.
PROiQ86TX2.
SOURCEiSearch...

Gene expression databases

BgeeiQ86TX2.
CleanExiHS_ACOT1.
ExpressionAtlasiQ86TX2. baseline.
GenevestigatoriQ86TX2.

Family and domain databases

Gene3Di3.40.50.1820. 2 hits.
InterProiIPR029058. AB_hydrolase.
IPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamiPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFiPIRSF016521. Acyl-CoA_hydro. 1 hit.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs."
    Hunt M.C., Rautanen A., Westin M.A.K., Svensson L.T., Alexson S.E.H.
    FASEB J. 20:1855-1864(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
  2. "Full-length cDNA libraries and normalization."
    Li W.B., Gruber C., Jessee J., Polayes D.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Neuroblastoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Bienvenut W.V.
    Submitted (DEC-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12; 25-35 AND 223-233, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Ovarian carcinoma.

Entry informationi

Entry nameiACOT1_HUMAN
AccessioniPrimary (citable) accession number: Q86TX2
Secondary accession number(s): A1L173, Q3I5F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.