Q86TX2 (ACOT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acyl-coenzyme A thioesterase 1
Short name=Acyl-CoA thioesterase 1
EC=3.1.2.2

Alternative name(s):
CTE-I
CTE-Ib
Inducible cytosolic acyl-coenzyme A thioester hydrolase
Long chain acyl-CoA thioester hydrolase
Short name=Long chain acyl-CoA hydrolase

Gene names

Name:	ACOT1
Synonyms:	CTE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active towards fatty acyl-CoA with chain-lengths of C12-C16 By similarity.
Catalytic activity	Palmitoyl-CoA + H2O = CoA + palmitate.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Ref.1.
Sequence similarities	Belongs to the C/M/P thioester hydrolase family.
Biophysicochemical properties	Kinetic parameters: KM=35.8 µM for C10-acyl-CoA Ref.1 KM=3.6 µM for C12-acyl-CoA KM=2.8 µM for C14-acyl-CoA KM=3.6 µM for C16-acyl-CoA KM=2.4 µM for C18-acyl-CoA KM=2 µM for C20-acyl-CoA KM=2.4 µM for C16:1-acyl-CoA KM=4.1 µM for C18:1-acyl-CoA KM=2.1 µM for C18:1-trans-acyl-CoA Vmax=224 nmol/min/mg enzyme toward C10-acyl-CoA Vmax=700 nmol/min/mg enzyme toward C12-acyl-CoA Vmax=912 nmol/min/mg enzyme toward C14-acyl-CoA Vmax=691 nmol/min/mg enzyme toward C16-acyl-CoA Vmax=597 nmol/min/mg enzyme toward C18-acyl-CoA Vmax=520 nmol/min/mg enzyme toward C20-acyl-CoA Vmax=577 nmol/min/mg enzyme toward C16:1-acyl-CoA Vmax=258 nmol/min/mg enzyme toward C18:1-acyl-CoA Vmax=309 nmol/min/mg enzyme toward C18:1-trans-acyl-CoA

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acyl-CoA metabolic process Inferred from direct assay Ref.1. Source: HGNC long-chain fatty acid metabolic process Inferred from direct assay Ref.1. Source: HGNC very long-chain fatty acid metabolic process Inferred from direct assay Ref.1. Source: HGNC
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: HGNC mitochondrion Inferred from direct assay. Source: HPA
Molecular_function	acyl-CoA hydrolase activity Inferred from direct assay Ref.1. Source: HGNC carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW palmitoyl-CoA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 421	420	Acyl-coenzyme A thioesterase 1		PRO_0000202155
Sites
	Active site	232	1	Charge relay system By similarity
	Active site	326	1	Charge relay system By similarity
	Active site	360	1	Charge relay system By similarity
	Site	2	1	Not acetylated
Natural variations
	Natural variant	266	1	R → H. Corresponds to variant rs1049568 [ dbSNP | Ensembl ].		VAR_059830

Sequences

	Sequence		Length	Mass (Da)
	Q86TX2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 04675F28D9D53B97	FASTA	421	46,277
	10 20 30 40 50 60 MAATLILEPA GRCCWDEPVR IAVRGLAPEQ PVTLRASLRD EKGALFQAHA RYRADTLGEL 70 80 90 100 110 120 DLERAPALGG SFAGLEPMGL LWALEPEKPL VRLVKRDVRT PLAVELEVLD GHDPDPGRLL 130 140 150 160 170 180 CRVRHERYFL PPGVRREPVR AGRVRGTLFL PPEPGPFPGI VDMFGTGGGL LEYRASLLAG 190 200 210 220 230 240 KGFAVMALAY YNYEDLPKTM ETLHLEYFEE AVNYLLSHPE VKGPGVGLLG ISKGGELCLS 250 260 270 280 290 300 MASFLKGITA AVVINGSVAN VGGTLRYKGE TLPPVGVNRN RIKVTKDGYA DIVDVLNSPL 310 320 330 340 350 360 EGPDQKSFIP VERAESTFLF LVGQDDHNWK SEFYANEACK RLQAHGRRKP QIICYPETGH 370 380 390 400 410 420 YIEPPYFPLC RASLHALVGS PIIWGGEPRA HAMAQVDAWK QLQTFFHKHL GGHEGTIPSK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs." Hunt M.C., Rautanen A., Westin M.A.K., Svensson L.T., Alexson S.E.H. FASEB J. 20:1855-1864(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
[2]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neuroblastoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Bienvenut W.V. Submitted (JAN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-12; 25-35 AND 223-233, CLEAVAGE OF INITIATOR METHIONINE, LACK OF N-TERMINAL ACETYLATION, MASS SPECTROMETRY. Tissue: Ovarian carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ082754 mRNA. Translation: AAZ31236.1. BX161396 mRNA. Translation: CAD61883.1. BC127748 mRNA. Translation: AAI27749.1. BC132889 mRNA. Translation: AAI32890.1. BC132891 mRNA. Translation: AAI32892.1. BC143042 mRNA. Translation: AAI43043.1.
IPI	IPI00333838.
RefSeq	NP_001032238.1. NM_001037161.1.
UniGene	Hs.568046.
3D structure databases
ProteinModelPortal	Q86TX2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000311224.
PTM databases
PhosphoSite	Q86TX2.
Polymorphism databases
DMDM	50428913.
2D gel databases
UCD-2DPAGE	Q86TX2.
Proteomic databases
PaxDb	Q86TX2.
PRIDE	Q86TX2.
Protocols and materials databases
DNASU	641371.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000311148; ENSP00000311224; ENSG00000184227.
GeneID	641371.
KEGG	hsa:641371.
UCSC	uc001xol.1. human.
Organism-specific databases
CTD	641371.
GeneCards	GC14P074003.
HGNC	HGNC:33128. ACOT1.
HPA	HPA043705.
MIM	614313. gene.
neXtProt	NX_Q86TX2.
PharmGKB	PA162375318.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1073.
HOGENOM	HOG000116219.
HOVERGEN	HBG000331.
InParanoid	Q86TX2.
KO	K01068.
OMA	GALHYKG.
OrthoDB	EOG4QC15F.
PhylomeDB	Q86TX2.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14105.
BRENDA	3.1.2.2. 2681.
SABIO-RK	Q86TX2.
Gene expression databases
ArrayExpress	Q86TX2.
Bgee	Q86TX2.
CleanEx	HS_ACOT1.
Genevestigator	Q86TX2.
GermOnline	ENSG00000184227. Homo sapiens.
Family and domain databases
InterPro	IPR016662. Acyl-CoA_thioEstase_long-chain. IPR014940. BAAT_C. IPR006862. Thio_Ohase/aa_AcTrfase. [Graphical view]
Pfam	PF08840. BAAT_C. 1 hit. PF04775. Bile_Hydr_Trans. 1 hit. [Graphical view]
PIRSF	PIRSF016521. Acyl-CoA_hydro. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	ACOT1. human.
GenomeRNAi	641371.
NextBio	113125.
SOURCE	Search...

Entry information

Entry name

ACOT1_HUMAN

Accession

Primary (citable) accession number: Q86TX2
Secondary accession number(s): A1L173, Q3I5F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.