Q86TN4 (TRPT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA 2'-phosphotransferase 1
EC=2.7.1.160

Gene names

Name:

TRPT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate Probable. Ref.1
Catalytic activity	2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.
Tissue specificity	Widely expressed. Weakly or not expressed in lung, spleen, small intestine and peripheral blood leukocytes. Ref.1
Sequence similarities	Belongs to the KptA/TPT1 family.

Ontologies

Keywords
Biological process	tRNA processing
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	NAD
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: InterPro
Molecular_function	tRNA 2'-phosphotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q86TN4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q86TN4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q86TN4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     187-224: DGIPFFRSANGVILTPGNTDGFLLPKYFKEALQLRPTR → G

Note: No experimental confirmation available.

Isoform 4 (identifier: Q86TN4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     109-109: Q → QVG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	tRNA 2'-phosphotransferase 1		PRO_0000273363
Amino acid modifications
	Modified residue	240	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	1 – 49	49	Missing in isoform 2.		VSP_022524
	Alternative sequence	109	1	Q → QVG in isoform 4.		VSP_045827
	Alternative sequence	187 – 224	38	DGIPF…LRPTR → G in isoform 3.		VSP_045273
	Natural variant	3	1	F → L. Corresponds to variant rs12788168 [ dbSNP | Ensembl ].		VAR_030134
	Natural variant	172	1	H → R. Ref.1 Ref.4 Corresponds to variant rs1059440 [ dbSNP | Ensembl ].		VAR_030135
	Natural variant	221	1	R → C. Corresponds to variant rs11549690 [ dbSNP | Ensembl ].		VAR_030136

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6209F45DC3DB5CBF	FASTA	253	27,742
	10 20 30 40 50 60 MNFSGGGRQE AAGSRGRRAP RPREQDRDVQ LSKALSYALR HGALKLGLPM GADGFVPLGT 70 80 90 100 110 120 LLQLPQFRGF SAEDVQRVVD TNRKQRFALQ LGDPSTGLLI RANQGHSLQV PKLELMPLET 130 140 150 160 170 180 PQALPPMLVH GTFWKHWPSI LLKGLSCQGR THIHLAPGLP GDPGIISGMR SHCEIAVFID 190 200 210 220 230 240 GPLALADGIP FFRSANGVIL TPGNTDGFLL PKYFKEALQL RPTRKPLSLA GDEETECQSS 250 PKHSSRERRR IQQ
	Isoform 2 [UniParc]. Checksum: 5B7E72C0D6F702E4	FASTA	204	22,427
	10 20 30 40 50 60 MGADGFVPLG TLLQLPQFRG FSAEDVQRVV DTNRKQRFAL QLGDPSTGLL IRANQGHSLQ 70 80 90 100 110 120 VPKLELMPLE TPQALPPMLV HGTFWKHWPS ILLKGLSCQG RTHIHLAPGL PGDPGIISGM 130 140 150 160 170 180 RSHCEIAVFI DGPLALADGI PFFRSANGVI LTPGNTDGFL LPKYFKEALQ LRPTRKPLSL 190 200 AGDEETECQS SPKHSSRERR RIQQ
	Isoform 3 [UniParc]. Checksum: 5DA5CBDEAD3EC3EC	FASTA	216	23,566
	10 20 30 40 50 60 MNFSGGGRQE AAGSRGRRAP RPREQDRDVQ LSKALSYALR HGALKLGLPM GADGFVPLGT 70 80 90 100 110 120 LLQLPQFRGF SAEDVQRVVD TNRKQRFALQ LGDPSTGLLI RANQGHSLQV PKLELMPLET 130 140 150 160 170 180 PQALPPMLVH GTFWKHWPSI LLKGLSCQGR THIHLAPGLP GDPGIISGMR SHCEIAVFID 190 200 210 GPLALAGKPL SLAGDEETEC QSSPKHSSRE RRRIQQ
	Isoform 4 [UniParc]. Checksum: 6759213814CCB3BF	FASTA	255	27,898
	10 20 30 40 50 60 MNFSGGGRQE AAGSRGRRAP RPREQDRDVQ LSKALSYALR HGALKLGLPM GADGFVPLGT 70 80 90 100 110 120 LLQLPQFRGF SAEDVQRVVD TNRKQRFALQ LGDPSTGLLI RANQGHSLQV GVPKLELMPL 130 140 150 160 170 180 ETPQALPPML VHGTFWKHWP SILLKGLSCQ GRTHIHLAPG LPGDPGIISG MRSHCEIAVF 190 200 210 220 230 240 IDGPLALADG IPFFRSANGV ILTPGNTDGF LLPKYFKEAL QLRPTRKPLS LAGDEETECQ 250 SSPKHSSRER RRIQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis." Hu Q.-D., Lu H., Huo K., Ying K., Li J., Xie Y., Mao Y., Li Y.-Y. Cell. Mol. Life Sci. 60:1725-1732(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, VARIANT ARG-172. Tissue: Fetal brain.
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANT ARG-172. Tissue: Epidermal carcinoma and Ovary.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[6]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-240, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY211494 mRNA. Translation: AAO62941.1. AP005668 Genomic DNA. No translation available. AP006334 Genomic DNA. No translation available. CH471076 Genomic DNA. Translation: EAW74202.1. BC005133 mRNA. Translation: AAH05133.1. BQ672032 mRNA. No translation available.
IPI	IPI00328580. IPI00651758. IPI00930269. IPI00930313.
RefSeq	NP_001028850.2. NM_001033678.3. NP_001153861.1. NM_001160389.1. NP_001153862.1. NM_001160390.1. NP_001153864.1. NM_001160392.1. NP_001153865.1. NM_001160393.1. NP_113660.1. NM_031472.3.
UniGene	Hs.326586.
3D structure databases
ProteinModelPortal	Q86TN4.
SMR	Q86TN4. Positions 29-218.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q86TN4. 1 interaction.
MINT	MINT-1485014.
STRING	9606.ENSP00000314073.
PTM databases
PhosphoSite	Q86TN4.
Polymorphism databases
DMDM	124053420.
Proteomic databases
PaxDb	Q86TN4.
PRIDE	Q86TN4.
Protocols and materials databases
DNASU	83707.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000317459; ENSP00000314073; ENSG00000149743. ENST00000394546; ENSP00000378050; ENSG00000149743. ENST00000394547; ENSP00000378051; ENSG00000149743. ENST00000541278; ENSP00000438683; ENSG00000149743.
GeneID	83707.
KEGG	hsa:83707.
UCSC	uc001nyn.3. human.
Organism-specific databases
CTD	83707.
GeneCards	GC11M063991.
HGNC	HGNC:20316. TRPT1.
HPA	HPA038706.
MIM	610470. gene.
neXtProt	NX_Q86TN4.
PharmGKB	PA134964265.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1859.
HOGENOM	HOG000229875.
HOVERGEN	HBG092612.
InParanoid	Q86TN4.
KO	K10669.
OrthoDB	EOG44F6BB.
PhylomeDB	Q86TN4.
Enzyme and pathway databases
BRENDA	2.7.1.160. 2681.
Gene expression databases
ArrayExpress	Q86TN4.
Bgee	Q86TN4.
CleanEx	HS_TRPT1.
Genevestigator	Q86TN4.
Family and domain databases
InterPro	IPR002745. Ptrans_KptA/Tpt1. [Graphical view]
Pfam	PF01885. PTS_2-RNA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	83707.
NextBio	72697.
SOURCE	Search...

Entry information

Entry name

TRPT1_HUMAN

Accession

Primary (citable) accession number: Q86TN4
Secondary accession number(s): A8MU17 , A8MYC9, F5H2B2, Q9BSB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.