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Protein

Probable ATP-dependent RNA helicase DDX53

Gene

DDX53

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei244ATP; via carbonyl oxygen1
Binding sitei249ATP1
Binding sitei311ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 273ATP6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX53 (EC:3.6.4.13)
Alternative name(s):
Cancer-associated gene protein
Cancer/testis antigen 26
Short name:
CT26
DEAD box protein 53
DEAD box protein CAGE
Gene namesi
Name:DDX53
Synonyms:CAGE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:20083. DDX53.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi168400.
OpenTargetsiENSG00000184735.
PharmGKBiPA134863598.

Polymorphism and mutation databases

BioMutaiDDX53.
DMDMi143811384.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549731 – 631Probable ATP-dependent RNA helicase DDX53Add BLAST631

Proteomic databases

EPDiQ86TM3.
PaxDbiQ86TM3.
PeptideAtlasiQ86TM3.
PRIDEiQ86TM3.

PTM databases

iPTMnetiQ86TM3.
PhosphoSitePlusiQ86TM3.

Expressioni

Tissue specificityi

Expressed in testis. Wide expression in various cancer tissues and cancer cell lines.1 Publication

Gene expression databases

BgeeiENSG00000184735.
CleanExiHS_DDX53.
GenevisibleiQ86TM3. HS.

Interactioni

Protein-protein interaction databases

BioGridi127960. 4 interactors.
STRINGi9606.ENSP00000368667.

Structurei

Secondary structure

1631
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi204 – 207Combined sources4
Beta strandi210 – 212Combined sources3
Helixi223 – 227Combined sources5
Helixi231 – 240Combined sources10
Helixi247 – 257Combined sources11
Beta strandi262 – 265Combined sources4
Helixi272 – 284Combined sources13
Beta strandi298 – 302Combined sources5
Helixi306 – 319Combined sources14
Beta strandi326 – 329Combined sources4
Helixi339 – 343Combined sources5
Beta strandi347 – 351Combined sources5
Helixi353 – 361Combined sources9
Beta strandi372 – 375Combined sources4
Helixi378 – 383Combined sources6
Helixi387 – 396Combined sources10
Beta strandi402 – 408Combined sources7
Helixi412 – 419Combined sources8
Beta strandi426 – 429Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IUYX-ray2.40A/B204-430[»]
ProteinModelPortaliQ86TM3.
SMRiQ86TM3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ86TM3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 109KHPROSITE-ProRule annotationAdd BLAST62
Domaini253 – 428Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST176
Domaini440 – 601Helicase C-terminalPROSITE-ProRule annotationAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi222 – 250Q motifAdd BLAST29
Motifi376 – 379DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 KH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0336. Eukaryota.
ENOG410XR3V. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiQ86TM3.
OMAiWEKRKWA.
OrthoDBiEOG091G05VF.
PhylomeDBiQ86TM3.
TreeFamiTF312949.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54791. SSF54791. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50084. KH_TYPE_1. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q86TM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP
60 70 80 90 100
LCFKIKNNMV GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE
110 120 130 140 150
MKAKAKAAIE TLIRKQESYN SESSVDNAAS QTPIGRNLGR NDIVGEAEPL
160 170 180 190 200
SNWDRIRAAV VECEKRKWAD LPPVKKNFYI ESKATSCMSE MQVINWRKEN
210 220 230 240 250
FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV GIVKPTPIQS
260 270 280 290 300
QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML
310 320 330 340 350
VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII
360 370 380 390 400
ATPGRLNDLQ MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD
410 420 430 440 450
RQTVMTSATW PDTVRQLALS YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE
460 470 480 490 500
KEKRALTQEF VENMSPNDKV IMFVSQKHIA DDLSSDFNIQ GISAESLHGN
510 520 530 540 550
SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY NYDFPRNIDV
560 570 580 590 600
YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM
610 620 630
AEQYKLNQQK RHRETRSRKP GQRRKEFYFL S
Length:631
Mass (Da):71,154
Last modified:April 3, 2007 - v3
Checksum:i27E72027AADAA193
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti243V → L in AAH67878 (PubMed:15489334).Curated1
Sequence conflicti557 – 569YIGRT…GTSVT → SLDGQERLHISS in AAK72102 (PubMed:11922625).CuratedAdd BLAST13
Sequence conflicti619K → E in AAH67878 (PubMed:15489334).Curated1
Sequence conflicti622Q → R in AAH51703 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05216862V → A.Corresponds to variant rs4412516dbSNPEnsembl.1
Natural variantiVAR_064707141N → T Found in a renal cell carcinoma case; somatic mutation. 1 Publication1
Natural variantiVAR_052169381M → I.Corresponds to variant rs5925720dbSNPEnsembl.1
Natural variantiVAR_052170391R → M.Corresponds to variant rs5926203dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039237 mRNA. Translation: AAK72102.1.
BC051703 mRNA. Translation: AAH51703.1.
BC067878 mRNA. Translation: AAH67878.1.
CCDSiCCDS35214.1.
PIRiJC7831.
RefSeqiNP_874358.2. NM_182699.3.
UniGeneiHs.434416.

Genome annotation databases

EnsembliENST00000327968; ENSP00000368667; ENSG00000184735.
GeneIDi168400.
KEGGihsa:168400.
UCSCiuc004daj.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY039237 mRNA. Translation: AAK72102.1.
BC051703 mRNA. Translation: AAH51703.1.
BC067878 mRNA. Translation: AAH67878.1.
CCDSiCCDS35214.1.
PIRiJC7831.
RefSeqiNP_874358.2. NM_182699.3.
UniGeneiHs.434416.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IUYX-ray2.40A/B204-430[»]
ProteinModelPortaliQ86TM3.
SMRiQ86TM3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127960. 4 interactors.
STRINGi9606.ENSP00000368667.

PTM databases

iPTMnetiQ86TM3.
PhosphoSitePlusiQ86TM3.

Polymorphism and mutation databases

BioMutaiDDX53.
DMDMi143811384.

Proteomic databases

EPDiQ86TM3.
PaxDbiQ86TM3.
PeptideAtlasiQ86TM3.
PRIDEiQ86TM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327968; ENSP00000368667; ENSG00000184735.
GeneIDi168400.
KEGGihsa:168400.
UCSCiuc004daj.4. human.

Organism-specific databases

CTDi168400.
DisGeNETi168400.
GeneCardsiDDX53.
HGNCiHGNC:20083. DDX53.
neXtProtiNX_Q86TM3.
OpenTargetsiENSG00000184735.
PharmGKBiPA134863598.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0336. Eukaryota.
ENOG410XR3V. LUCA.
GeneTreeiENSGT00820000126976.
HOGENOMiHOG000268804.
HOVERGENiHBG015893.
InParanoidiQ86TM3.
OMAiWEKRKWA.
OrthoDBiEOG091G05VF.
PhylomeDBiQ86TM3.
TreeFamiTF312949.

Miscellaneous databases

EvolutionaryTraceiQ86TM3.
GenomeRNAii168400.
PROiQ86TM3.

Gene expression databases

BgeeiENSG00000184735.
CleanExiHS_DDX53.
GenevisibleiQ86TM3. HS.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00013. KH_1. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00322. KH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54791. SSF54791. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50084. KH_TYPE_1. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX53_HUMAN
AccessioniPrimary (citable) accession number: Q86TM3
Secondary accession number(s): Q0D2N2, Q6NVV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: April 3, 2007
Last modified: November 2, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.