Reviewed, UniProtKB/Swiss-Prot Q86TM3 (DDX53_HUMAN)

Names and origin

Protein names

Recommended name:
    Probable ATP-dependent RNA helicase DDX53
    EC=3.6.1.-
Alternative name(s):
    DEAD box protein 53
    DEAD box protein CAGE
    Cancer-associated gene protein
    Cancer/testis antigen 26
      Short name=CT26

Gene names

Name:	DDX53
Synonyms:	CAGE

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	631 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Nucleus. Ref.1
Tissue specificity	Expressed in testis. Wide expression in various cancer tissues and cancer cell lines. Ref.1
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 KH domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	Probable ATP-dependent RNA helicase DDX53		PRO_0000054973
Regions
	Domain	48 – 109	62	KH
	Domain	253 – 428	176	Helicase ATP-binding
	Domain	440 – 601	162	Helicase C-terminal
	Nucleotide binding	266 – 273	8	ATP By similarity
	Motif	222 – 250	29	Q motif
	Motif	376 – 379	4	DEAD box
Natural variations
	Natural variant	62	1	V → A: dbSNP rs4412516.		VAR_052168
	Natural variant	381	1	M → I: dbSNP rs5925720.		VAR_052169
	Natural variant	391	1	R → M: dbSNP rs5926203.		VAR_052170
Experimental info
	Sequence conflict	243	1	V → L in AAH67878. Ref.2
	Sequence conflict	557 – 569	13	YIGRT…GTSVT → SLDGQERLHISS in AAK72102. Ref.1
	Sequence conflict	619	1	K → E in AAH67878. Ref.2
	Sequence conflict	622	1	Q → R in AAH51703. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q86TM3-1 [UniParc]. Last modified April 3, 2007. Version 3. Checksum: 27E72027AADAA193	FASTA	631	71,154
	10 20 30 40 50 60 MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP LCFKIKNNMV 70 80 90 100 110 120 GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE MKAKAKAAIE TLIRKQESYN 130 140 150 160 170 180 SESSVDNAAS QTPIGRNLGR NDIVGEAEPL SNWDRIRAAV VECEKRKWAD LPPVKKNFYI 190 200 210 220 230 240 ESKATSCMSE MQVINWRKEN FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV 250 260 270 280 290 300 GIVKPTPIQS QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML 310 320 330 340 350 360 VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII ATPGRLNDLQ 370 380 390 400 410 420 MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD RQTVMTSATW PDTVRQLALS 430 440 450 460 470 480 YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE KEKRALTQEF VENMSPNDKV IMFVSQKHIA 490 500 510 520 530 540 DDLSSDFNIQ GISAESLHGN SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY 550 560 570 580 590 600 NYDFPRNIDV YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM 610 620 630 AEQYKLNQQK RHRETRSRKP GQRRKEFYFL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel cancer/testis antigen gene CAGE." Cho B., Lim Y., Lee D.Y., Park S.Y., Lee H., Kim W.H., Yang H., Bang Y.J., Jeoung D.I. Biochem. Biophys. Res. Commun. 292:715-726(2002) [PubMed: 11922625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY039237 mRNA. Translation: AAK72102.1. BC051703 mRNA. Translation: AAH51703.1. BC067878 mRNA. Translation: AAH67878.1.
IPI	IPI00328813.
PIR	JC7831.
RefSeq	NP_874358.2.
UniGene	Hs.434416
3D structure databases
HSSP	HSSP built from PDB template 1QDE based on UniProtKB P10081.
ModBase	Search...
Proteomic databases
PRIDE	Q86TM3.
Genome annotation databases
Ensembl	ENSG00000184735. Homo sapiens. [Contig view]
GeneID	168400.
KEGG	hsa:168400.
Organism-specific databases
GeneCards	GC0XP022927.
HGNC	HGNC:20083. DDX53.
HPA	HPA003186.
PharmGKB	PA134863598.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q86TM3.
HOVERGEN	Q86TM3.
OMA	Q86TM3. SREPPLC.
Gene expression databases
ArrayExpress	Q86TM3.
Bgee	Q86TM3.
CleanEx	HS_DDX53.
GermOnline	ENSG00000184735. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. PF00013. KH_1. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. SM00322. KH. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS50084. KH_TYPE_1. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	88718.

Entry information

Entry name

DDX53_HUMAN

Accession

Primary (citable) accession number: Q86TM3
Secondary accession number(s): Q0D2N2, Q6NVV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	April 3, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)