Reviewed, UniProtKB/Swiss-Prot Q86TL0 (ATG4D_HUMAN)

Names and origin

Protein names

Recommended name:
    Cysteine protease ATG4D
    EC=3.4.22.-
Alternative name(s):
    Autophagy-related protein 4 homolog D
      Short name=Autophagin-4
    Autophagy-related cysteine endopeptidase 4
    AUT-like 4 cysteine endopeptidase

Gene names

Name:	ATG4D
Synonyms:	APG4D, AUTL4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
Enzyme regulation	Inhibited by N-ethylmaleimide.
Subcellular location	Cytoplasm Probable.
Tissue specificity	Mainly expressed in skeletal muscle and, to a lower extent, in testis. Ref.1
Sequence similarities	Belongs to the peptidase C54 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological process	autophagy Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein targeting to vacuole Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB microtubule associated complex Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	cysteine-type endopeptidase activity Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Cysteine protease ATG4D		PRO_0000215853
Sites
	Active site	144	1	Nucleophile By similarity
	Active site	356	1	Potential
	Active site	358	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q86TL0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DBD91A3F1F80D2B8	FASTA	474	52,922
	10 20 30 40 50 60 MNSVSPAAAQ YRSSSPEDAR RRPEARRPRG PRGPDPNGLG PSGASGPALG SPGAGPSEPD 70 80 90 100 110 120 EVDKFKAKFL TAWNNVKYGW VVKSRTSFSK ISSIHLCGRR YRFEGEGDIQ RFQRDFVSRL 130 140 150 160 170 180 WLTYRRDFPP LPGGCLTSDC GWGCMLRSGQ MMLAQGLLLH FLPRDWTWAE GMGLGPPELS 190 200 210 220 230 240 GSASPSRYHG PARWMPPRWA QGAPELEQER RHRQIVSWFA DHPRAPFGLH RLVELGQSSG 250 260 270 280 290 300 KKAGDWYGPS LVAHILRKAV ESCSDVTRLV VYVSQDCTVY KADVARLVAR PDPTAEWKSV 310 320 330 340 350 360 VILVPVRLGG ETLNPVYVPC VKELLRCELC LGIMGGKPRH SLYFIGYQDD FLLYLDPHYC 370 380 390 400 410 420 QPTVDVSQAD FPLESFHCTS PRKMAFAKMD PSCTVGFYAG DRKEFETLCS ELTRVLSSSS 430 440 450 460 470 ATERYPMFTL AEGHAQDHSL DDLCSQLAQP TLRLPRTGRL LRAKRPSSED FVFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." Marino G., Uria J.A., Puente X.S., Quesada V., Bordallo J., Lopez-Otin C. J. Biol. Chem. 278:3671-3678(2003) [PubMed: 12446702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Fetal liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ312332 mRNA. Translation: CAC85951.1. BC068992 mRNA. Translation: AAH68992.1.
IPI	IPI00328573.
RefSeq	NP_116274.3.
UniGene	Hs.512799
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C54.005.
PTM databases
PhosphoSite	Q86TL0.
Proteomic databases
PRIDE	Q86TL0.
Genome annotation databases
Ensembl	ENSG00000130734. Homo sapiens. [Contig view]
GeneID	84971.
KEGG	hsa:84971.
Organism-specific databases
GeneCards	GC19P010518.
H-InvDB	HIX0014748.
HGNC	HGNC:20789. ATG4D.
MIM	611340. gene.
PharmGKB	PA134907475.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q86TL0.
HOVERGEN	Q86TL0.
OMA	Q86TL0. CLTSDCG.
Gene expression databases
ArrayExpress	Q86TL0.
Bgee	Q86TL0.
CleanEx	HS_ATG4D.
GermOnline	ENSG00000130734. Homo sapiens.
Family and domain databases
InterPro	IPR005078. Peptidase_C54. [Graphical view]
PANTHER	PTHR22624. Peptidase_C54. 1 hit.
Pfam	PF03416. Peptidase_C54. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	75518.
SOURCE	Search...

Entry information

Entry name

ATG4D_HUMAN

Accession

Primary (citable) accession number: Q86TL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)