ID   SRCA_HUMAN              Reviewed;         473 AA.
AC   Q86TD4; B4DYT9;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-OCT-2020, sequence version 3.
DT   24-JAN-2024, entry version 141.
DE   RecName: Full=Sarcalumenin;
DE   Flags: Precursor;
GN   Name=SRL;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 6-473 (ISOFORM 1).
RC   TISSUE=Skeletal muscle;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15616553; DOI=10.1038/nature03187;
RA   Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA   Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA   Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA   Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA   Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA   Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA   Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA   Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA   Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA   Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA   Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA   Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA   Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA   Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA   Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA   Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA   Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA   Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA   Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA   DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA   Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA   Myers R.M., Rubin E.M., Pennacchio L.A.;
RT   "The sequence and analysis of duplication-rich human chromosome 16.";
RL   Nature 432:988-994(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
CC   -!- INTERACTION:
CC       Q86TD4-2; Q9Y2W7: KCNIP3; NbExp=3; IntAct=EBI-12304565, EBI-751501;
CC       Q86TD4-2; O43765: SGTA; NbExp=3; IntAct=EBI-12304565, EBI-347996;
CC       Q86TD4-2; P62760: VSNL1; NbExp=3; IntAct=EBI-12304565, EBI-740943;
CC   -!- SUBCELLULAR LOCATION: Sarcoplasmic reticulum lumen
CC       {ECO:0000250|UniProtKB:P13666}. Sarcoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:P13666}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P13666}. Note=May associate with the
CC       sarcoplasmic reticulum membrane, via a calcium-dependent mechanism.
CC       {ECO:0000250|UniProtKB:P13666}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q86TD4-2; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q86TD4-1; Sequence=VSP_060763;
CC       Name=3;
CC         IsoId=Q86TD4-3; Sequence=VSP_060762;
CC   -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:P13666}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC       superfamily. Dynamin/Fzo/YdjA family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01055}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD89901.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AL831973; CAD89901.1; ALT_FRAME; mRNA.
DR   EMBL; AL832361; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BX647476; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AC009171; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AK302601; BAG63851.1; -; mRNA.
DR   CCDS; CCDS42113.1; -. [Q86TD4-2]
DR   CCDS; CCDS81943.1; -. [Q86TD4-3]
DR   RefSeq; NP_001092284.1; NM_001098814.1. [Q86TD4-2]
DR   RefSeq; NP_001310596.1; NM_001323667.1. [Q86TD4-3]
DR   RefSeq; NP_001310597.1; NM_001323668.1. [Q86TD4-3]
DR   RefSeq; XP_016879017.1; XM_017023528.1. [Q86TD4-3]
DR   RefSeq; XP_016879018.1; XM_017023529.1. [Q86TD4-3]
DR   AlphaFoldDB; Q86TD4; -.
DR   SMR; Q86TD4; -.
DR   BioGRID; 112249; 5.
DR   IntAct; Q86TD4; 5.
DR   STRING; 9606.ENSP00000382518; -.
DR   GlyCosmos; Q86TD4; 2 sites, No reported glycans.
DR   GlyGen; Q86TD4; 2 sites.
DR   iPTMnet; Q86TD4; -.
DR   PhosphoSitePlus; Q86TD4; -.
DR   BioMuta; SRL; -.
DR   DMDM; 85540609; -.
DR   MassIVE; Q86TD4; -.
DR   PaxDb; 9606-ENSP00000382518; -.
DR   PeptideAtlas; Q86TD4; -.
DR   ProteomicsDB; 5546; -.
DR   ProteomicsDB; 69686; -. [Q86TD4-1]
DR   ProteomicsDB; 69687; -. [Q86TD4-2]
DR   Antibodypedia; 55440; 50 antibodies from 13 providers.
DR   DNASU; 6345; -.
DR   Ensembl; ENST00000399609.7; ENSP00000382518.3; ENSG00000185739.13. [Q86TD4-2]
DR   Ensembl; ENST00000537996.1; ENSP00000440350.1; ENSG00000185739.13. [Q86TD4-3]
DR   GeneID; 6345; -.
DR   KEGG; hsa:6345; -.
DR   MANE-Select; ENST00000399609.7; ENSP00000382518.3; NM_001098814.2; NP_001092284.1.
DR   UCSC; uc002cvz.4; human. [Q86TD4-2]
DR   UCSC; uc059qck.1; human.
DR   AGR; HGNC:11295; -.
DR   CTD; 6345; -.
DR   DisGeNET; 6345; -.
DR   GeneCards; SRL; -.
DR   HGNC; HGNC:11295; SRL.
DR   HPA; ENSG00000185739; Group enriched (heart muscle, skeletal muscle, tongue).
DR   MIM; 604992; gene.
DR   neXtProt; NX_Q86TD4; -.
DR   OpenTargets; ENSG00000185739; -.
DR   PharmGKB; PA36119; -.
DR   VEuPathDB; HostDB:ENSG00000185739; -.
DR   eggNOG; KOG1954; Eukaryota.
DR   GeneTree; ENSGT00940000157834; -.
DR   InParanoid; Q86TD4; -.
DR   OrthoDB; 12127at2759; -.
DR   PhylomeDB; Q86TD4; -.
DR   TreeFam; TF324008; -.
DR   PathwayCommons; Q86TD4; -.
DR   SignaLink; Q86TD4; -.
DR   BioGRID-ORCS; 6345; 11 hits in 1140 CRISPR screens.
DR   ChiTaRS; SRL; human.
DR   GeneWiki; Sarcalumenin; -.
DR   GenomeRNAi; 6345; -.
DR   Pharos; Q86TD4; Tdark.
DR   PRO; PR:Q86TD4; -.
DR   Proteomes; UP000005640; Chromosome 16.
DR   RNAct; Q86TD4; Protein.
DR   Bgee; ENSG00000185739; Expressed in hindlimb stylopod muscle and 148 other cell types or tissues.
DR   ExpressionAtlas; Q86TD4; baseline and differential.
DR   GO; GO:0033018; C:sarcoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR   CDD; cd09913; EHD; 1.
DR   Gene3D; 1.10.268.20; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR045063; Dynamin_N.
DR   InterPro; IPR031692; EHD_N.
DR   InterPro; IPR030381; G_DYNAMIN_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR43681:SF1; SARCALUMENIN; 1.
DR   PANTHER; PTHR43681; TRANSMEMBRANE GTPASE FZO; 1.
DR   Pfam; PF00350; Dynamin_N; 1.
DR   Pfam; PF16880; EHD_N; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51718; G_DYNAMIN_2; 1.
DR   Genevisible; Q86TD4; HS.
PE   1: Evidence at protein level;
KW   Alternative splicing; Glycoprotein; Membrane; Reference proteome;
KW   Sarcoplasmic reticulum; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..473
FT                   /note="Sarcalumenin"
FT                   /id="PRO_0000045426"
FT   DOMAIN          90..331
FT                   /note="Dynamin-type G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          100..107
FT                   /note="G1 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          128..129
FT                   /note="G2 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          190..193
FT                   /note="G3 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          255..258
FT                   /note="G4 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   REGION          278
FT                   /note="G5 motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT   CARBOHYD        281
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        389
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..42
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_060762"
FT   VAR_SEQ         20
FT                   /note="A -> AELQVSASGGTEDVGNLLENHFSAGDASLEEKERALYADTAPQDKKL
FT                   LLHYPDGREAESPKKTPASAASAGPDPEASLSNASATESPPPGERDDRDAAGPGEEKNG
FT                   PPVASALPPGGAKGPVEEEWPEPSSGEGQGEEETGFGLPTEGTASGEAGGQAGGHELPE
FT                   EVQEVQGDSLVQGAVAGTAEPKAEGASPHSEGDGVGPLNAEAEGSPGPGEEPAVPEGAP
FT                   DVAAVGGESEPDIDTQASEGTEDQGEPGPAAEASAEPGGAQSVKAGDTEESQAPEMTEE
FT                   DADEASSEEESGDGSGSEEEGGVPSEEESEEDSGDGASSEEAEGASEEATEPQEAGEPQ
FT                   EAREPQEGGDLQEAEESQEGGDPQEAEEPQEGGAPQEGGEPQEGGDPQEAREPQEAREP
FT                   QEGAELPEATGTTSHRDRGAQPGPEELNTESMGSETLDMKAEEPEEGHQGRESPIIVAQ
FT                   (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_060763"
FT   CONFLICT        416
FT                   /note="L -> P (in Ref. 1; CAD89901)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   473 AA;  54431 MW;  976721235C129CB7 CRC64;
     MRALVLLGCL LASLLFSGQA EETEDANEEA PLRDRSHIEK TLMLNEDKPS DDYSAVLQRL
     RKIYHSSIKP LEQSYKYNEL RQHEITDGEI TSKPMVLFLG PWSVGKSTMI NYLLGLENTR
     YQLYTGAEPT TSEFTVLMHG PKLKTIEGIV MAADSARSFS PLEKFGQNFL EKLIGIEVPH
     KLLERVTFVD TPGIIENRKQ QERGYPFNDV CQWFIDRADL IFVVFDPTKL DVGLELEMLF
     RQLKGRESQI RIILNKADNL ATQMLMRVYG ALFWSLAPLI NVTEPPRVYV SSFWPQEYKP
     DTHQELFLQE EISLLEDLNQ VIENRLENKI AFIRQHAIRV RIHALLVDRY LQTYKDKMTF
     FSDGELVFKD IVEDPDKFYI FKTILAKTNV SKFDLPNREA YKDFFGINPI SSFKLLSQQC
     SYMGGCFLEK IERAITQELP GLLGSLGLGK NPGALNCDKT GCSETPKNRY RKH
//